Irradiation Dose-response Effects on Angiogenesis and Involvement of Nitric Oxide
Background: Recent results implicate nitric oxide (NO) in the irradiation- induced perturbation of angiogenic mechanisms. We have previously shown, using the chick embryo chorioallantoic membrane (CAM) and a dose of 10 Gy, that the NO pathway is involved in X-ray-induced anti-angiogenicity. In the c...
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| Veröffentlicht in: | Anticancer research 2005-03, Vol.25 (2A), p.1059-1065 |
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| creator | HADJIMICHAEL, Christos KARDAMAKIS, Dimitrios PAPAIOANNOU, Stamatis |
| description | Background: Recent results implicate nitric oxide (NO) in the irradiation- induced perturbation of angiogenic mechanisms.
We have previously shown, using the chick embryo chorioallantoic membrane (CAM) and a dose of 10 Gy, that the NO pathway is
involved in X-ray-induced anti-angiogenicity. In the current study, we investigated the implication of NO in the effects of
various doses (2-15 Gy) of irradiation on angiogenesis. Materials and Methods: X-irradiation, NO synthase inhibitors, NO releasing
agent and the in vivo CAM angiogenesis model (disc and ring method) were used. The CAM areas were irradiated on the 9th or
the 14th day of embryo development and the vascular density was determined morphologically. Results: Doses up to 5 Gy resulted
in dose-dependent reduction of vascular density, whereas the NO synthase inhibitors, added immediately post-irradiation, protected
the 9-day CAM from the above anti-angiogenic effects of irradiation. NO donor, under certain circumstances potentiated the
effects of X-rays when applied to the 9-day CAM using the ring method. Conclusion: These results confirm the implication of
NO in the anti-angiogenic mechanism of X-rays and describe the dose-response pattern of NO involvement in this action. |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_pasca</sourceid><recordid>TN_cdi_pubmed_primary_15868946</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>15868946</sourcerecordid><originalsourceid>FETCH-LOGICAL-h270t-dc5ef3573244479cb51a9d0d5ad29bc726b2f63aa746f3e1e220a9a1375d50023</originalsourceid><addsrcrecordid>eNpFz8tKAzEYBeAgiq3VV5BsdDeQyySZLEutWigWQdfDP7m0kZlMScaqb-9AK67O4nwcOGdoSpWmhRKcnKMpYYIUihAxQVc5fxAipa74JZpQUclKl3KKXlcpgQ0whD7ihz67Irm872N2eOm9M0PGYzGP29BvXXQ5ZAzR4lU89O3BdS4OuPf4JQwpGLz5DtZdowsPbXY3p5yh98fl2-K5WG-eVov5utgxRYbCGuE8F4qzsiyVNo2goC2xAizTjVFMNsxLDqBK6bmjjjECGihXwgpCGJ-h2-Pu_rPpnK33KXSQfuq_byO4OwHIBlqfIJqQ_51UlFRajO7-6HZhu_sKydW5g7YdZ3kNiYmazWtKhOa_Hh9lrw</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Irradiation Dose-response Effects on Angiogenesis and Involvement of Nitric Oxide</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>HADJIMICHAEL, Christos ; KARDAMAKIS, Dimitrios ; PAPAIOANNOU, Stamatis</creator><creatorcontrib>HADJIMICHAEL, Christos ; KARDAMAKIS, Dimitrios ; PAPAIOANNOU, Stamatis</creatorcontrib><description>Background: Recent results implicate nitric oxide (NO) in the irradiation- induced perturbation of angiogenic mechanisms.
We have previously shown, using the chick embryo chorioallantoic membrane (CAM) and a dose of 10 Gy, that the NO pathway is
involved in X-ray-induced anti-angiogenicity. In the current study, we investigated the implication of NO in the effects of
various doses (2-15 Gy) of irradiation on angiogenesis. Materials and Methods: X-irradiation, NO synthase inhibitors, NO releasing
agent and the in vivo CAM angiogenesis model (disc and ring method) were used. The CAM areas were irradiated on the 9th or
the 14th day of embryo development and the vascular density was determined morphologically. Results: Doses up to 5 Gy resulted
in dose-dependent reduction of vascular density, whereas the NO synthase inhibitors, added immediately post-irradiation, protected
the 9-day CAM from the above anti-angiogenic effects of irradiation. NO donor, under certain circumstances potentiated the
effects of X-rays when applied to the 9-day CAM using the ring method. Conclusion: These results confirm the implication of
NO in the anti-angiogenic mechanism of X-rays and describe the dose-response pattern of NO involvement in this action.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 15868946</identifier><language>eng</language><publisher>Attiki: International Institute of Anticancer Research</publisher><subject>Animals ; Arginine - pharmacology ; Biological and medical sciences ; Chick Embryo ; Chorioallantoic Membrane - blood supply ; Chorioallantoic Membrane - drug effects ; Dose-Response Relationship, Radiation ; Enzyme Inhibitors - pharmacology ; Medical sciences ; Neovascularization, Physiologic - drug effects ; Neovascularization, Physiologic - physiology ; Neovascularization, Physiologic - radiation effects ; NG-Nitroarginine Methyl Ester - pharmacology ; Nitric Oxide - antagonists & inhibitors ; Nitric Oxide - metabolism ; Nitric Oxide - physiology ; Nitric Oxide Donors - pharmacology ; Nitric Oxide Synthase - antagonists & inhibitors ; Nitroarginine - pharmacology ; Nitroprusside - pharmacology ; Tumors ; X-Rays</subject><ispartof>Anticancer research, 2005-03, Vol.25 (2A), p.1059-1065</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16710895$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15868946$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HADJIMICHAEL, Christos</creatorcontrib><creatorcontrib>KARDAMAKIS, Dimitrios</creatorcontrib><creatorcontrib>PAPAIOANNOU, Stamatis</creatorcontrib><title>Irradiation Dose-response Effects on Angiogenesis and Involvement of Nitric Oxide</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Background: Recent results implicate nitric oxide (NO) in the irradiation- induced perturbation of angiogenic mechanisms.
We have previously shown, using the chick embryo chorioallantoic membrane (CAM) and a dose of 10 Gy, that the NO pathway is
involved in X-ray-induced anti-angiogenicity. In the current study, we investigated the implication of NO in the effects of
various doses (2-15 Gy) of irradiation on angiogenesis. Materials and Methods: X-irradiation, NO synthase inhibitors, NO releasing
agent and the in vivo CAM angiogenesis model (disc and ring method) were used. The CAM areas were irradiated on the 9th or
the 14th day of embryo development and the vascular density was determined morphologically. Results: Doses up to 5 Gy resulted
in dose-dependent reduction of vascular density, whereas the NO synthase inhibitors, added immediately post-irradiation, protected
the 9-day CAM from the above anti-angiogenic effects of irradiation. NO donor, under certain circumstances potentiated the
effects of X-rays when applied to the 9-day CAM using the ring method. Conclusion: These results confirm the implication of
NO in the anti-angiogenic mechanism of X-rays and describe the dose-response pattern of NO involvement in this action.</description><subject>Animals</subject><subject>Arginine - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Chick Embryo</subject><subject>Chorioallantoic Membrane - blood supply</subject><subject>Chorioallantoic Membrane - drug effects</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Medical sciences</subject><subject>Neovascularization, Physiologic - drug effects</subject><subject>Neovascularization, Physiologic - physiology</subject><subject>Neovascularization, Physiologic - radiation effects</subject><subject>NG-Nitroarginine Methyl Ester - pharmacology</subject><subject>Nitric Oxide - antagonists & inhibitors</subject><subject>Nitric Oxide - metabolism</subject><subject>Nitric Oxide - physiology</subject><subject>Nitric Oxide Donors - pharmacology</subject><subject>Nitric Oxide Synthase - antagonists & inhibitors</subject><subject>Nitroarginine - pharmacology</subject><subject>Nitroprusside - pharmacology</subject><subject>Tumors</subject><subject>X-Rays</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFz8tKAzEYBeAgiq3VV5BsdDeQyySZLEutWigWQdfDP7m0kZlMScaqb-9AK67O4nwcOGdoSpWmhRKcnKMpYYIUihAxQVc5fxAipa74JZpQUclKl3KKXlcpgQ0whD7ihz67Irm872N2eOm9M0PGYzGP29BvXXQ5ZAzR4lU89O3BdS4OuPf4JQwpGLz5DtZdowsPbXY3p5yh98fl2-K5WG-eVov5utgxRYbCGuE8F4qzsiyVNo2goC2xAizTjVFMNsxLDqBK6bmjjjECGihXwgpCGJ-h2-Pu_rPpnK33KXSQfuq_byO4OwHIBlqfIJqQ_51UlFRajO7-6HZhu_sKydW5g7YdZ3kNiYmazWtKhOa_Hh9lrw</recordid><startdate>20050301</startdate><enddate>20050301</enddate><creator>HADJIMICHAEL, Christos</creator><creator>KARDAMAKIS, Dimitrios</creator><creator>PAPAIOANNOU, Stamatis</creator><general>International Institute of Anticancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20050301</creationdate><title>Irradiation Dose-response Effects on Angiogenesis and Involvement of Nitric Oxide</title><author>HADJIMICHAEL, Christos ; KARDAMAKIS, Dimitrios ; PAPAIOANNOU, Stamatis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h270t-dc5ef3573244479cb51a9d0d5ad29bc726b2f63aa746f3e1e220a9a1375d50023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Arginine - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Chick Embryo</topic><topic>Chorioallantoic Membrane - blood supply</topic><topic>Chorioallantoic Membrane - drug effects</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Medical sciences</topic><topic>Neovascularization, Physiologic - drug effects</topic><topic>Neovascularization, Physiologic - physiology</topic><topic>Neovascularization, Physiologic - radiation effects</topic><topic>NG-Nitroarginine Methyl Ester - pharmacology</topic><topic>Nitric Oxide - antagonists & inhibitors</topic><topic>Nitric Oxide - metabolism</topic><topic>Nitric Oxide - physiology</topic><topic>Nitric Oxide Donors - pharmacology</topic><topic>Nitric Oxide Synthase - antagonists & inhibitors</topic><topic>Nitroarginine - pharmacology</topic><topic>Nitroprusside - pharmacology</topic><topic>Tumors</topic><topic>X-Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HADJIMICHAEL, Christos</creatorcontrib><creatorcontrib>KARDAMAKIS, Dimitrios</creatorcontrib><creatorcontrib>PAPAIOANNOU, Stamatis</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HADJIMICHAEL, Christos</au><au>KARDAMAKIS, Dimitrios</au><au>PAPAIOANNOU, Stamatis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Irradiation Dose-response Effects on Angiogenesis and Involvement of Nitric Oxide</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2005-03-01</date><risdate>2005</risdate><volume>25</volume><issue>2A</issue><spage>1059</spage><epage>1065</epage><pages>1059-1065</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Background: Recent results implicate nitric oxide (NO) in the irradiation- induced perturbation of angiogenic mechanisms.
We have previously shown, using the chick embryo chorioallantoic membrane (CAM) and a dose of 10 Gy, that the NO pathway is
involved in X-ray-induced anti-angiogenicity. In the current study, we investigated the implication of NO in the effects of
various doses (2-15 Gy) of irradiation on angiogenesis. Materials and Methods: X-irradiation, NO synthase inhibitors, NO releasing
agent and the in vivo CAM angiogenesis model (disc and ring method) were used. The CAM areas were irradiated on the 9th or
the 14th day of embryo development and the vascular density was determined morphologically. Results: Doses up to 5 Gy resulted
in dose-dependent reduction of vascular density, whereas the NO synthase inhibitors, added immediately post-irradiation, protected
the 9-day CAM from the above anti-angiogenic effects of irradiation. NO donor, under certain circumstances potentiated the
effects of X-rays when applied to the 9-day CAM using the ring method. Conclusion: These results confirm the implication of
NO in the anti-angiogenic mechanism of X-rays and describe the dose-response pattern of NO involvement in this action.</abstract><cop>Attiki</cop><pub>International Institute of Anticancer Research</pub><pmid>15868946</pmid><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Arginine - pharmacology Biological and medical sciences Chick Embryo Chorioallantoic Membrane - blood supply Chorioallantoic Membrane - drug effects Dose-Response Relationship, Radiation Enzyme Inhibitors - pharmacology Medical sciences Neovascularization, Physiologic - drug effects Neovascularization, Physiologic - physiology Neovascularization, Physiologic - radiation effects NG-Nitroarginine Methyl Ester - pharmacology Nitric Oxide - antagonists & inhibitors Nitric Oxide - metabolism Nitric Oxide - physiology Nitric Oxide Donors - pharmacology Nitric Oxide Synthase - antagonists & inhibitors Nitroarginine - pharmacology Nitroprusside - pharmacology Tumors X-Rays |
| title | Irradiation Dose-response Effects on Angiogenesis and Involvement of Nitric Oxide |
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