Structural characteristics of hen egg ovalbumin expressed in yeast Pichia pastoris

The recombinant ovalbumin (OVA) produced in yeast Pichia pastoris was purified from the culture medium by anion exchange chromatography, and its structural characteristics were compared with those of hen egg OVA, mainly from the point of view of post-translational modification. The expressed OVA con...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2005-04, Vol.69 (4), p.755-761
Hauptverfasser:	Ito, K. (Yamaguchi Univ. (Japan). Faculty of Agriculture), Matsudomi, N
Format:	Artikel
Sprache:	eng
Schlagworte:	ACETYLATION ALBUMINS Amino Acid Sequence AMINO ACID SEQUENCES Animals Biological and medical sciences BIOTECHNOLOGY Chickens Circular Dichroism EGG WHITE Fundamental and applied biological sciences. Psychology GENE EXPRESSION Genetic Vectors - genetics Glycosylation Mass Spectrometry N-linked glycosylation ovalbumin Ovalbumin - biosynthesis Ovalbumin - chemistry Ovalbumin - genetics Ovalbumin - isolation & purification Ovum PHOSPHORYLATION Pichia - genetics Pichia - metabolism PICHIA PASTORIS Pichia pastoris expression Protein Denaturation Protein Folding RECOMBINANT PROTEINS Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Spectrometry, Fluorescence Temperature TRYPTOPHAN
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