Cloning and sequence analysis of the micronuclear and macronuclear gene encoding Rab protein of Euplotes octocarinatus
The DNA in a micronucleus undergoes remarkable rearrangements when it develops into a macronucleus after cell mating in the hypotrichous ciliate. A Rab gene was isolated from the macronuclear plasmid mini-library of Euplotes octocarinatus. A micronuclear version of the Rab gene was amplified by poly...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2005-03, Vol.69 (3), p.649-652 |
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| creator | Wang, W. (Shanxi Univ., Taiyuan (China)) Zhi, H Chai, B Liang, A |
| description | The DNA in a micronucleus undergoes remarkable rearrangements when it develops into a macronucleus after cell mating in the hypotrichous ciliate. A Rab gene was isolated from the macronuclear plasmid mini-library of Euplotes octocarinatus. A micronuclear version of the Rab gene was amplified by polymerase chain reaction (PCR). The macronuclear DNA molecule carrying the Rab gene is 767 bp long and shows characteristics typical of macronuclear chromosomes of hypotrichous ciliates. Three of the five cysteines are encoded by the opal codon UGA. The deduced protein is a 207-amino acid (aa) with a molecular mass of 23kDa. The protein shares 36% identity with Rab 1 protein of Plasmodium and yeast Analysis of the sequences indicated that the micronuclear version of the Rab gene contains two internal eliminated sequences, internal eliminated sequence (IES)1 and IES2. IES1 is flanked by a pair of hepta-nucleotide 5'-AAATTTT-3' direct repeats, and IES2 is flanked by 5'-TA-3' direct repeats. |
| doi_str_mv | 10.1271/bbb.69.649 |
| format | Article |
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(Shanxi Univ., Taiyuan (China)) ; Zhi, H ; Chai, B ; Liang, A</creator><creatorcontrib>Wang, W. (Shanxi Univ., Taiyuan (China)) ; Zhi, H ; Chai, B ; Liang, A</creatorcontrib><description>The DNA in a micronucleus undergoes remarkable rearrangements when it develops into a macronucleus after cell mating in the hypotrichous ciliate. A Rab gene was isolated from the macronuclear plasmid mini-library of Euplotes octocarinatus. A micronuclear version of the Rab gene was amplified by polymerase chain reaction (PCR). The macronuclear DNA molecule carrying the Rab gene is 767 bp long and shows characteristics typical of macronuclear chromosomes of hypotrichous ciliates. Three of the five cysteines are encoded by the opal codon UGA. The deduced protein is a 207-amino acid (aa) with a molecular mass of 23kDa. The protein shares 36% identity with Rab 1 protein of Plasmodium and yeast Analysis of the sequences indicated that the micronuclear version of the Rab gene contains two internal eliminated sequences, internal eliminated sequence (IES)1 and IES2. IES1 is flanked by a pair of hepta-nucleotide 5'-AAATTTT-3' direct repeats, and IES2 is flanked by 5'-TA-3' direct repeats.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.69.649</identifier><identifier>PMID: 15785000</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Cell Nucleus - metabolism ; ciliates ; CILIOPHORA ; CLONING ; Cloning, Molecular ; DNA ; DNA, Protozoan ; Euplotes - genetics ; Euplotes octocarinatus ; Fundamental and applied biological sciences. Psychology ; GENES ; Genes, Protozoan ; internal eliminated sequences ; Molecular Sequence Data ; NUCLEOTIDE SEQUENCE ; NUCLEUS ; PCR ; PROTEINS ; Rab gene ; rab GTP-Binding Proteins - chemistry ; rab GTP-Binding Proteins - genetics ; rab GTP-Binding Proteins - metabolism</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2005-03, Vol.69 (3), p.649-652</ispartof><rights>2005 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2005</rights><rights>2005 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c568t-5ed442b2727b90ecc49eaf94390948805351aaeff92fe012d211d275901259183</citedby><cites>FETCH-LOGICAL-c568t-5ed442b2727b90ecc49eaf94390948805351aaeff92fe012d211d275901259183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16797292$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15785000$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, W. (Shanxi Univ., Taiyuan (China))</creatorcontrib><creatorcontrib>Zhi, H</creatorcontrib><creatorcontrib>Chai, B</creatorcontrib><creatorcontrib>Liang, A</creatorcontrib><title>Cloning and sequence analysis of the micronuclear and macronuclear gene encoding Rab protein of Euplotes octocarinatus</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>The DNA in a micronucleus undergoes remarkable rearrangements when it develops into a macronucleus after cell mating in the hypotrichous ciliate. A Rab gene was isolated from the macronuclear plasmid mini-library of Euplotes octocarinatus. A micronuclear version of the Rab gene was amplified by polymerase chain reaction (PCR). The macronuclear DNA molecule carrying the Rab gene is 767 bp long and shows characteristics typical of macronuclear chromosomes of hypotrichous ciliates. Three of the five cysteines are encoded by the opal codon UGA. The deduced protein is a 207-amino acid (aa) with a molecular mass of 23kDa. The protein shares 36% identity with Rab 1 protein of Plasmodium and yeast Analysis of the sequences indicated that the micronuclear version of the Rab gene contains two internal eliminated sequences, internal eliminated sequence (IES)1 and IES2. IES1 is flanked by a pair of hepta-nucleotide 5'-AAATTTT-3' direct repeats, and IES2 is flanked by 5'-TA-3' direct repeats.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Nucleus - metabolism</subject><subject>ciliates</subject><subject>CILIOPHORA</subject><subject>CLONING</subject><subject>Cloning, Molecular</subject><subject>DNA</subject><subject>DNA, Protozoan</subject><subject>Euplotes - genetics</subject><subject>Euplotes octocarinatus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENES</subject><subject>Genes, Protozoan</subject><subject>internal eliminated sequences</subject><subject>Molecular Sequence Data</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>NUCLEUS</subject><subject>PCR</subject><subject>PROTEINS</subject><subject>Rab gene</subject><subject>rab GTP-Binding Proteins - chemistry</subject><subject>rab GTP-Binding Proteins - genetics</subject><subject>rab GTP-Binding Proteins - metabolism</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd1rFDEUxYModlt98V0ZEH0QZk0y-Zg8lqXVloIi-hzuZJJ1SiZZkxll_3sz7kqLCH1K7uV3zr3JQegFwWtCJXnfdd1aqLVg6hFakYbJWigmH6MVVkTULePkBJ3mfItxaXDyFJ0QLlteyhX6ufExDGFbQeirbH_MNhhbCvD7POQqumr6bqtxMCmG2XgL6Q85wr3G1gZbFV3sF6Mv0FW7FCc7hEV-Me98KYqVmaKBNASY5vwMPXHgs31-PM_Qt8uLr5uP9c2nD1eb85vacNFONbc9Y7SjkspOYWsMUxacYo3CirUt5g0nANY5RZ3FhPaUkJ5KrsqdK9I2Z-jtwbdsVN6WJz0O2VjvIdg4Zy0kbySn4kGQSNEophbw9T_gbZxT-a_CMKZaQqRc5r47UOWbck7W6V0aRkh7TbBeQtMlNC2ULqEV-NXRcu5G29-hx5QK8OYIQDbgXYJghnzHCakkVbRw_MANwcU0wq-YfK8n2PuY_oqa_y7w8qBzEDVsU8GuP1OMy3DZMtL8BhtWuio</recordid><startdate>20050301</startdate><enddate>20050301</enddate><creator>Wang, W. (Shanxi Univ., Taiyuan (China))</creator><creator>Zhi, H</creator><creator>Chai, B</creator><creator>Liang, A</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20050301</creationdate><title>Cloning and sequence analysis of the micronuclear and macronuclear gene encoding Rab protein of Euplotes octocarinatus</title><author>Wang, W. (Shanxi Univ., Taiyuan (China)) ; Zhi, H ; Chai, B ; Liang, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c568t-5ed442b2727b90ecc49eaf94390948805351aaeff92fe012d211d275901259183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Nucleus - metabolism</topic><topic>ciliates</topic><topic>CILIOPHORA</topic><topic>CLONING</topic><topic>Cloning, Molecular</topic><topic>DNA</topic><topic>DNA, Protozoan</topic><topic>Euplotes - genetics</topic><topic>Euplotes octocarinatus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENES</topic><topic>Genes, Protozoan</topic><topic>internal eliminated sequences</topic><topic>Molecular Sequence Data</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>NUCLEUS</topic><topic>PCR</topic><topic>PROTEINS</topic><topic>Rab gene</topic><topic>rab GTP-Binding Proteins - chemistry</topic><topic>rab GTP-Binding Proteins - genetics</topic><topic>rab GTP-Binding Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, W. (Shanxi Univ., Taiyuan (China))</creatorcontrib><creatorcontrib>Zhi, H</creatorcontrib><creatorcontrib>Chai, B</creatorcontrib><creatorcontrib>Liang, A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, W. (Shanxi Univ., Taiyuan (China))</au><au>Zhi, H</au><au>Chai, B</au><au>Liang, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and sequence analysis of the micronuclear and macronuclear gene encoding Rab protein of Euplotes octocarinatus</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2005-03-01</date><risdate>2005</risdate><volume>69</volume><issue>3</issue><spage>649</spage><epage>652</epage><pages>649-652</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>The DNA in a micronucleus undergoes remarkable rearrangements when it develops into a macronucleus after cell mating in the hypotrichous ciliate. A Rab gene was isolated from the macronuclear plasmid mini-library of Euplotes octocarinatus. A micronuclear version of the Rab gene was amplified by polymerase chain reaction (PCR). The macronuclear DNA molecule carrying the Rab gene is 767 bp long and shows characteristics typical of macronuclear chromosomes of hypotrichous ciliates. Three of the five cysteines are encoded by the opal codon UGA. The deduced protein is a 207-amino acid (aa) with a molecular mass of 23kDa. The protein shares 36% identity with Rab 1 protein of Plasmodium and yeast Analysis of the sequences indicated that the micronuclear version of the Rab gene contains two internal eliminated sequences, internal eliminated sequence (IES)1 and IES2. IES1 is flanked by a pair of hepta-nucleotide 5'-AAATTTT-3' direct repeats, and IES2 is flanked by 5'-TA-3' direct repeats.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>15785000</pmid><doi>10.1271/bbb.69.649</doi><tpages>4</tpages></addata></record> |
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| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Freely Accessible Japanese Titles; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Cell Nucleus - metabolism ciliates CILIOPHORA CLONING Cloning, Molecular DNA DNA, Protozoan Euplotes - genetics Euplotes octocarinatus Fundamental and applied biological sciences. Psychology GENES Genes, Protozoan internal eliminated sequences Molecular Sequence Data NUCLEOTIDE SEQUENCE NUCLEUS PCR PROTEINS Rab gene rab GTP-Binding Proteins - chemistry rab GTP-Binding Proteins - genetics rab GTP-Binding Proteins - metabolism |
| title | Cloning and sequence analysis of the micronuclear and macronuclear gene encoding Rab protein of Euplotes octocarinatus |
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