Evaluation of a Multi-parameter Biomarker Set for Oxidative Damage in Man: Increased Urinary Excretion of Lipid, Protein and DNA Oxidation Products after One Hour of Exercise
The objective of the present study was to evaluate a comprehensive set of urinary biomarkers for oxidative damage to lipids, proteins and DNA, in man. Eighteen moderately trained males (mean age 24.6±0.7) exercised 60 min at 70% of maximal O2 uptake on a cycle ergometer. Urine fractions for 12 h wer...
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| Veröffentlicht in: | Free radical research 2004-12, Vol.38 (12), p.1269-1279 |
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| creator | Orhan, Hilmi van Holland, Berry Krab, Betty Moeken, Janine Vermeulen, Nico P.E. Hollander, Peter Meerman, John H.N. |
| description | The objective of the present study was to evaluate a comprehensive set of urinary biomarkers for oxidative damage to lipids, proteins and DNA, in man. Eighteen moderately trained males (mean age 24.6±0.7) exercised 60 min at 70% of maximal O2 uptake on a cycle ergometer. Urine fractions for 12 h were collected 1 day before, and for 3 consecutive days after exercise.
As biomarkers of lipid peroxidation, 8 aldehydes (i.e. propanal, butanal, pentanal, hexanal, heptanal, octanal, nonanal and malondialdehyde-MDA)and acetone were analyzed in urines by gas chromatography with electron capture detection (GC-ECD). As a biomarker of protein oxidation, o,o′-dityrosine was analyzed in urine samples by a recently developed isotope dilution HPLC-atmospheric pressure chemical ionization (APCI)-tandem-mass spectrometry (HPLC-APCI-MS/MS) methodology. As a biomarker of oxidative DNA damage, urinary excretion of 8-hydroxy-2′-deoxyguanosine (8-OHdG) was measured by an ELISA method.
On the day of exercise, significant increases were observed in urinary excretions of acetone ( p |
| doi_str_mv | 10.1080/10715760400013763 |
| format | Article |
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As biomarkers of lipid peroxidation, 8 aldehydes (i.e. propanal, butanal, pentanal, hexanal, heptanal, octanal, nonanal and malondialdehyde-MDA)and acetone were analyzed in urines by gas chromatography with electron capture detection (GC-ECD). As a biomarker of protein oxidation, o,o′-dityrosine was analyzed in urine samples by a recently developed isotope dilution HPLC-atmospheric pressure chemical ionization (APCI)-tandem-mass spectrometry (HPLC-APCI-MS/MS) methodology. As a biomarker of oxidative DNA damage, urinary excretion of 8-hydroxy-2′-deoxyguanosine (8-OHdG) was measured by an ELISA method.
On the day of exercise, significant increases were observed in urinary excretions of acetone ( p<0.025, n=18) and butanal ( p<0.01, n=18) in the 12 h daytime fractions compared to the daytime fraction before exercise. The urinary acetone excretion was also significantly ( p<0.05) increased on the 1st day after exercise. Octanal and nonanal were increased in the daytime urine fraction on the 2nd day after exercise. However, these increases were of borderline significance ( p=0.09 and p=0.07, respectively).
Significantly elevated urinary o,o′-dityrosine amounts were observed in the daytime fraction on the day of exercise ( p<0.025) and on the 1st day after exercise ( p=0.07) compared to the before exercise daytime fraction.
Excretion of urinary 8-OHdG was statistically significantly increased in the daytime fractions on the day of exercise ( p=0.07) and on the 1st day after exercise ( p<0.025) compared to before exercise daytime fraction.
Increases in urinary excretions of acetone, propanal, pentanal, MDA and 8-OHdG significantly correlated with training status (hours of exercise/week) of the volunteers, while o,o′-dityrosine did not.
To our knowledge, the present study is the first to evaluate a multi-parameter non-invasive biomarker set for damage to three main cellular targets of ROS. It shows that 1 h of exercise may already induce oxidative damage in moderately trained individuals and that the chosen urinary biomarkers are sensitive enough to monitor such damage.</description><identifier>ISSN: 1071-5762</identifier><identifier>EISSN: 1029-2470</identifier><identifier>DOI: 10.1080/10715760400013763</identifier><identifier>PMID: 15763951</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>8-hydroxy-2′-deoxyguanosine ; Acetone - urine ; Adult ; Aldehydes - urine ; Biomarker ; Biomarkers - urine ; Chromatography, Gas ; Chromatography, High Pressure Liquid ; Deoxyguanosine - analogs & derivatives ; Deoxyguanosine - urine ; DNA damage ; DNA Damage - physiology ; Enzyme-Linked Immunosorbent Assay ; Exercise ; Exercise - physiology ; Humans ; Lipid peroxidation ; Lipid Peroxidation - physiology ; Male ; o,o′-dityrosine ; Oxidation-Reduction ; Oxidative Stress - physiology ; Protein oxidation ; Proteins - metabolism ; Tyrosine - analogs & derivatives ; Tyrosine - urine</subject><ispartof>Free radical research, 2004-12, Vol.38 (12), p.1269-1279</ispartof><rights>2004 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-e8652e31fb8ff3731f7a910f50b3ebc80fc127d5fff5d4c6db9a58923a1128aa3</citedby><cites>FETCH-LOGICAL-c404t-e8652e31fb8ff3731f7a910f50b3ebc80fc127d5fff5d4c6db9a58923a1128aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/10715760400013763$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/10715760400013763$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,59620,60409,61194,61375</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15763951$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Orhan, Hilmi</creatorcontrib><creatorcontrib>van Holland, Berry</creatorcontrib><creatorcontrib>Krab, Betty</creatorcontrib><creatorcontrib>Moeken, Janine</creatorcontrib><creatorcontrib>Vermeulen, Nico P.E.</creatorcontrib><creatorcontrib>Hollander, Peter</creatorcontrib><creatorcontrib>Meerman, John H.N.</creatorcontrib><title>Evaluation of a Multi-parameter Biomarker Set for Oxidative Damage in Man: Increased Urinary Excretion of Lipid, Protein and DNA Oxidation Products after One Hour of Exercise</title><title>Free radical research</title><addtitle>Free Radic Res</addtitle><description>The objective of the present study was to evaluate a comprehensive set of urinary biomarkers for oxidative damage to lipids, proteins and DNA, in man. Eighteen moderately trained males (mean age 24.6±0.7) exercised 60 min at 70% of maximal O2 uptake on a cycle ergometer. Urine fractions for 12 h were collected 1 day before, and for 3 consecutive days after exercise.
As biomarkers of lipid peroxidation, 8 aldehydes (i.e. propanal, butanal, pentanal, hexanal, heptanal, octanal, nonanal and malondialdehyde-MDA)and acetone were analyzed in urines by gas chromatography with electron capture detection (GC-ECD). As a biomarker of protein oxidation, o,o′-dityrosine was analyzed in urine samples by a recently developed isotope dilution HPLC-atmospheric pressure chemical ionization (APCI)-tandem-mass spectrometry (HPLC-APCI-MS/MS) methodology. As a biomarker of oxidative DNA damage, urinary excretion of 8-hydroxy-2′-deoxyguanosine (8-OHdG) was measured by an ELISA method.
On the day of exercise, significant increases were observed in urinary excretions of acetone ( p<0.025, n=18) and butanal ( p<0.01, n=18) in the 12 h daytime fractions compared to the daytime fraction before exercise. The urinary acetone excretion was also significantly ( p<0.05) increased on the 1st day after exercise. Octanal and nonanal were increased in the daytime urine fraction on the 2nd day after exercise. However, these increases were of borderline significance ( p=0.09 and p=0.07, respectively).
Significantly elevated urinary o,o′-dityrosine amounts were observed in the daytime fraction on the day of exercise ( p<0.025) and on the 1st day after exercise ( p=0.07) compared to the before exercise daytime fraction.
Excretion of urinary 8-OHdG was statistically significantly increased in the daytime fractions on the day of exercise ( p=0.07) and on the 1st day after exercise ( p<0.025) compared to before exercise daytime fraction.
Increases in urinary excretions of acetone, propanal, pentanal, MDA and 8-OHdG significantly correlated with training status (hours of exercise/week) of the volunteers, while o,o′-dityrosine did not.
To our knowledge, the present study is the first to evaluate a multi-parameter non-invasive biomarker set for damage to three main cellular targets of ROS. It shows that 1 h of exercise may already induce oxidative damage in moderately trained individuals and that the chosen urinary biomarkers are sensitive enough to monitor such damage.</description><subject>8-hydroxy-2′-deoxyguanosine</subject><subject>Acetone - urine</subject><subject>Adult</subject><subject>Aldehydes - urine</subject><subject>Biomarker</subject><subject>Biomarkers - urine</subject><subject>Chromatography, Gas</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Deoxyguanosine - analogs & derivatives</subject><subject>Deoxyguanosine - urine</subject><subject>DNA damage</subject><subject>DNA Damage - physiology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Exercise</subject><subject>Exercise - physiology</subject><subject>Humans</subject><subject>Lipid peroxidation</subject><subject>Lipid Peroxidation - physiology</subject><subject>Male</subject><subject>o,o′-dityrosine</subject><subject>Oxidation-Reduction</subject><subject>Oxidative Stress - physiology</subject><subject>Protein oxidation</subject><subject>Proteins - metabolism</subject><subject>Tyrosine - analogs & derivatives</subject><subject>Tyrosine - urine</subject><issn>1071-5762</issn><issn>1029-2470</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAURiMEoqXwAGyQV6wItfPnBNiUdmgrTRkk6Nq6sa-pS2IPtlNmXopnxNFMhRBSV75yzvkcfTfLXjL6ltGWHjPKWc0bWlFKWcmb8lF2yGjR5UXF6eN55ixPQHGQPQvhdoaqmj_NDmar7Gp2mP1e3MEwQTTOEqcJkKtpiCZfg4cRI3ry0bgR_I80fcVItPNktTEqCXdIzmCE70iMJVdg35FLKz1CQEWuvbHgt2SxSTf32UuzNuoN-eJdxKSAVeTs88l9XGLSFzXJGAjo-eWVRXLhJj-7iw16aQI-z55oGAK-2J9H2fWnxbfTi3y5Or88PVnmsqJVzLFt6gJLpvtW65KngUPHqK5pX2IvW6olK7iqtda1qmSj-g7qtitKYKxoAcqj7PUud-3dzwlDFKMJEocBLLopiIYXTcuqNoFsB0rvQvCoxdqbVNhWMCrmJYn_lpScV_vwqR9R_TX2W0nAhx1gbCp8hF_OD0pE2A7Oaw82NSHKh_Lf_6PfIAzxRoJHcZv6tKm4B_7uD8c3s54</recordid><startdate>200412</startdate><enddate>200412</enddate><creator>Orhan, Hilmi</creator><creator>van Holland, Berry</creator><creator>Krab, Betty</creator><creator>Moeken, Janine</creator><creator>Vermeulen, Nico P.E.</creator><creator>Hollander, Peter</creator><creator>Meerman, John H.N.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200412</creationdate><title>Evaluation of a Multi-parameter Biomarker Set for Oxidative Damage in Man: Increased Urinary Excretion of Lipid, Protein and DNA Oxidation Products after One Hour of Exercise</title><author>Orhan, Hilmi ; van Holland, Berry ; Krab, Betty ; Moeken, Janine ; Vermeulen, Nico P.E. ; Hollander, Peter ; Meerman, John H.N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-e8652e31fb8ff3731f7a910f50b3ebc80fc127d5fff5d4c6db9a58923a1128aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>8-hydroxy-2′-deoxyguanosine</topic><topic>Acetone - urine</topic><topic>Adult</topic><topic>Aldehydes - urine</topic><topic>Biomarker</topic><topic>Biomarkers - urine</topic><topic>Chromatography, Gas</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Deoxyguanosine - analogs & derivatives</topic><topic>Deoxyguanosine - urine</topic><topic>DNA damage</topic><topic>DNA Damage - physiology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Exercise</topic><topic>Exercise - physiology</topic><topic>Humans</topic><topic>Lipid peroxidation</topic><topic>Lipid Peroxidation - physiology</topic><topic>Male</topic><topic>o,o′-dityrosine</topic><topic>Oxidation-Reduction</topic><topic>Oxidative Stress - physiology</topic><topic>Protein oxidation</topic><topic>Proteins - metabolism</topic><topic>Tyrosine - analogs & derivatives</topic><topic>Tyrosine - urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Orhan, Hilmi</creatorcontrib><creatorcontrib>van Holland, Berry</creatorcontrib><creatorcontrib>Krab, Betty</creatorcontrib><creatorcontrib>Moeken, Janine</creatorcontrib><creatorcontrib>Vermeulen, Nico P.E.</creatorcontrib><creatorcontrib>Hollander, Peter</creatorcontrib><creatorcontrib>Meerman, John H.N.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Free radical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Orhan, Hilmi</au><au>van Holland, Berry</au><au>Krab, Betty</au><au>Moeken, Janine</au><au>Vermeulen, Nico P.E.</au><au>Hollander, Peter</au><au>Meerman, John H.N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of a Multi-parameter Biomarker Set for Oxidative Damage in Man: Increased Urinary Excretion of Lipid, Protein and DNA Oxidation Products after One Hour of Exercise</atitle><jtitle>Free radical research</jtitle><addtitle>Free Radic Res</addtitle><date>2004-12</date><risdate>2004</risdate><volume>38</volume><issue>12</issue><spage>1269</spage><epage>1279</epage><pages>1269-1279</pages><issn>1071-5762</issn><eissn>1029-2470</eissn><abstract>The objective of the present study was to evaluate a comprehensive set of urinary biomarkers for oxidative damage to lipids, proteins and DNA, in man. Eighteen moderately trained males (mean age 24.6±0.7) exercised 60 min at 70% of maximal O2 uptake on a cycle ergometer. Urine fractions for 12 h were collected 1 day before, and for 3 consecutive days after exercise.
As biomarkers of lipid peroxidation, 8 aldehydes (i.e. propanal, butanal, pentanal, hexanal, heptanal, octanal, nonanal and malondialdehyde-MDA)and acetone were analyzed in urines by gas chromatography with electron capture detection (GC-ECD). As a biomarker of protein oxidation, o,o′-dityrosine was analyzed in urine samples by a recently developed isotope dilution HPLC-atmospheric pressure chemical ionization (APCI)-tandem-mass spectrometry (HPLC-APCI-MS/MS) methodology. As a biomarker of oxidative DNA damage, urinary excretion of 8-hydroxy-2′-deoxyguanosine (8-OHdG) was measured by an ELISA method.
On the day of exercise, significant increases were observed in urinary excretions of acetone ( p<0.025, n=18) and butanal ( p<0.01, n=18) in the 12 h daytime fractions compared to the daytime fraction before exercise. The urinary acetone excretion was also significantly ( p<0.05) increased on the 1st day after exercise. Octanal and nonanal were increased in the daytime urine fraction on the 2nd day after exercise. However, these increases were of borderline significance ( p=0.09 and p=0.07, respectively).
Significantly elevated urinary o,o′-dityrosine amounts were observed in the daytime fraction on the day of exercise ( p<0.025) and on the 1st day after exercise ( p=0.07) compared to the before exercise daytime fraction.
Excretion of urinary 8-OHdG was statistically significantly increased in the daytime fractions on the day of exercise ( p=0.07) and on the 1st day after exercise ( p<0.025) compared to before exercise daytime fraction.
Increases in urinary excretions of acetone, propanal, pentanal, MDA and 8-OHdG significantly correlated with training status (hours of exercise/week) of the volunteers, while o,o′-dityrosine did not.
To our knowledge, the present study is the first to evaluate a multi-parameter non-invasive biomarker set for damage to three main cellular targets of ROS. It shows that 1 h of exercise may already induce oxidative damage in moderately trained individuals and that the chosen urinary biomarkers are sensitive enough to monitor such damage.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>15763951</pmid><doi>10.1080/10715760400013763</doi><tpages>11</tpages></addata></record> |
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| subjects | 8-hydroxy-2′-deoxyguanosine Acetone - urine Adult Aldehydes - urine Biomarker Biomarkers - urine Chromatography, Gas Chromatography, High Pressure Liquid Deoxyguanosine - analogs & derivatives Deoxyguanosine - urine DNA damage DNA Damage - physiology Enzyme-Linked Immunosorbent Assay Exercise Exercise - physiology Humans Lipid peroxidation Lipid Peroxidation - physiology Male o,o′-dityrosine Oxidation-Reduction Oxidative Stress - physiology Protein oxidation Proteins - metabolism Tyrosine - analogs & derivatives Tyrosine - urine |
| title | Evaluation of a Multi-parameter Biomarker Set for Oxidative Damage in Man: Increased Urinary Excretion of Lipid, Protein and DNA Oxidation Products after One Hour of Exercise |
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