The effects of N(omega)-propyl-L-arginine on reperfusion injury of skeletal muscle
N(omega)-Propyl-L-arginine (NPA) is reported to be a highly selective inhibitor of neuronal nitric oxide synthase (nNOS). This in vivo study observed its role in ischemia/reperfusion (I/R) injury in rat skeletal muscle. Our results showed that NPA infusion significantly increased vessel diameters an...
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| Veröffentlicht in: | Nitric oxide 2004-08, Vol.11 (1), p.17 |
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| creator | Gowda, Charan Toomayan, Glen A Qi, Wen-Ning Chen, Long-En Cai, Yongting Allen, Diane M Seaber, Anthony V Urbaniak, James R |
| description | N(omega)-Propyl-L-arginine (NPA) is reported to be a highly selective inhibitor of neuronal nitric oxide synthase (nNOS). This in vivo study observed its role in ischemia/reperfusion (I/R) injury in rat skeletal muscle. Our results showed that NPA infusion significantly increased vessel diameters and blood flow in reperfused cremaster muscle, and slightly increased contractile function in reperfused extensor digitorum longus (EDL) muscle. In addition, NPA treatment slightly increased I/R-mediated downregulation of nNOS and eNOS mRNA and protein levels. Although NPA showed a beneficial role in I/R injury, our in vivo data do not support NPA as a selective nNOS inhibitor. Also, our data do not provide any insight into the mechanism of NPA. Thus, the in vivo mechanism of action of NPA needs to be further identified, and the role of nNOS in skeletal muscle I/R still remains to be determined. |
| doi_str_mv | 10.1016/j.niox.2004.07.007 |
| format | Article |
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This in vivo study observed its role in ischemia/reperfusion (I/R) injury in rat skeletal muscle. Our results showed that NPA infusion significantly increased vessel diameters and blood flow in reperfused cremaster muscle, and slightly increased contractile function in reperfused extensor digitorum longus (EDL) muscle. In addition, NPA treatment slightly increased I/R-mediated downregulation of nNOS and eNOS mRNA and protein levels. Although NPA showed a beneficial role in I/R injury, our in vivo data do not support NPA as a selective nNOS inhibitor. Also, our data do not provide any insight into the mechanism of NPA. Thus, the in vivo mechanism of action of NPA needs to be further identified, and the role of nNOS in skeletal muscle I/R still remains to be determined.</description><identifier>ISSN: 1089-8603</identifier><identifier>DOI: 10.1016/j.niox.2004.07.007</identifier><identifier>PMID: 15350553</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Arginine - administration & dosage ; Arginine - analogs & derivatives ; Arginine - pharmacology ; Arginine - therapeutic use ; Blood Pressure - drug effects ; Drug Evaluation, Preclinical ; Enzyme Induction - drug effects ; Enzyme Inhibitors - pharmacology ; Female ; Infusions, Intravenous ; Injections, Intra-Arterial ; Ischemia - drug therapy ; Ischemia - metabolism ; Ischemia - pathology ; Male ; Microcirculation - drug effects ; Muscle Contraction - drug effects ; Muscle, Skeletal - blood supply ; Muscle, Skeletal - drug effects ; Muscle, Skeletal - metabolism ; Nerve Tissue Proteins - biosynthesis ; Nerve Tissue Proteins - genetics ; Nitric Oxide Synthase - biosynthesis ; Nitric Oxide Synthase - genetics ; Nitric Oxide Synthase Type I ; Nitric Oxide Synthase Type III ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury - drug therapy ; Reperfusion Injury - prevention & control</subject><ispartof>Nitric oxide, 2004-08, Vol.11 (1), p.17</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15350553$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gowda, Charan</creatorcontrib><creatorcontrib>Toomayan, Glen A</creatorcontrib><creatorcontrib>Qi, Wen-Ning</creatorcontrib><creatorcontrib>Chen, Long-En</creatorcontrib><creatorcontrib>Cai, Yongting</creatorcontrib><creatorcontrib>Allen, Diane M</creatorcontrib><creatorcontrib>Seaber, Anthony V</creatorcontrib><creatorcontrib>Urbaniak, James R</creatorcontrib><title>The effects of N(omega)-propyl-L-arginine on reperfusion injury of skeletal muscle</title><title>Nitric oxide</title><addtitle>Nitric Oxide</addtitle><description>N(omega)-Propyl-L-arginine (NPA) is reported to be a highly selective inhibitor of neuronal nitric oxide synthase (nNOS). This in vivo study observed its role in ischemia/reperfusion (I/R) injury in rat skeletal muscle. Our results showed that NPA infusion significantly increased vessel diameters and blood flow in reperfused cremaster muscle, and slightly increased contractile function in reperfused extensor digitorum longus (EDL) muscle. In addition, NPA treatment slightly increased I/R-mediated downregulation of nNOS and eNOS mRNA and protein levels. Although NPA showed a beneficial role in I/R injury, our in vivo data do not support NPA as a selective nNOS inhibitor. Also, our data do not provide any insight into the mechanism of NPA. Thus, the in vivo mechanism of action of NPA needs to be further identified, and the role of nNOS in skeletal muscle I/R still remains to be determined.</description><subject>Animals</subject><subject>Arginine - administration & dosage</subject><subject>Arginine - analogs & derivatives</subject><subject>Arginine - pharmacology</subject><subject>Arginine - therapeutic use</subject><subject>Blood Pressure - drug effects</subject><subject>Drug Evaluation, Preclinical</subject><subject>Enzyme Induction - drug effects</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Female</subject><subject>Infusions, Intravenous</subject><subject>Injections, Intra-Arterial</subject><subject>Ischemia - drug therapy</subject><subject>Ischemia - metabolism</subject><subject>Ischemia - pathology</subject><subject>Male</subject><subject>Microcirculation - drug effects</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle, Skeletal - blood supply</subject><subject>Muscle, Skeletal - drug effects</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Nerve Tissue Proteins - biosynthesis</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nitric Oxide Synthase - biosynthesis</subject><subject>Nitric Oxide Synthase - genetics</subject><subject>Nitric Oxide Synthase Type I</subject><subject>Nitric Oxide Synthase Type III</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reperfusion Injury - drug therapy</subject><subject>Reperfusion Injury - prevention & control</subject><issn>1089-8603</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1j0tLxDAUhbNQnHH0D7iQLHWRetM0TbuUwRcUBel-SNt7x9a-SFqw_96KujoffJwDh7ErCYEEGd81QV8PX0EIEAVgAgBzwrYSklQkMagNO_e-gVWqJD5jG6mVBq3Vlr3nH8iRCMvJ84H4683Q4dHeitEN49KKTFh3rPu6Rz703OGIjmZfr1z3zeyWn47_xBYn2_Ju9mWLF-yUbOvx8i93LH98yPfPInt7etnfZ2JM5SQqHRMZkDJSqaHCSFtWK1UxGbsKshHJpDQQQoEAFIdJJAtIU1XaVKMFtWPXv7PjXHRYHUZXd9Yth_9v6ht0LVC_</recordid><startdate>200408</startdate><enddate>200408</enddate><creator>Gowda, Charan</creator><creator>Toomayan, Glen A</creator><creator>Qi, Wen-Ning</creator><creator>Chen, Long-En</creator><creator>Cai, Yongting</creator><creator>Allen, Diane M</creator><creator>Seaber, Anthony V</creator><creator>Urbaniak, James R</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>200408</creationdate><title>The effects of N(omega)-propyl-L-arginine on reperfusion injury of skeletal muscle</title><author>Gowda, Charan ; Toomayan, Glen A ; Qi, Wen-Ning ; Chen, Long-En ; Cai, Yongting ; Allen, Diane M ; Seaber, Anthony V ; Urbaniak, James R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p91t-d56ff70114397fb71acd397d6f7aff7fa4f18c7020be00f62841b0993ca95ea03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Arginine - administration & dosage</topic><topic>Arginine - analogs & derivatives</topic><topic>Arginine - pharmacology</topic><topic>Arginine - therapeutic use</topic><topic>Blood Pressure - drug effects</topic><topic>Drug Evaluation, Preclinical</topic><topic>Enzyme Induction - drug effects</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Female</topic><topic>Infusions, Intravenous</topic><topic>Injections, Intra-Arterial</topic><topic>Ischemia - drug therapy</topic><topic>Ischemia - metabolism</topic><topic>Ischemia - pathology</topic><topic>Male</topic><topic>Microcirculation - drug effects</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle, Skeletal - blood supply</topic><topic>Muscle, Skeletal - drug effects</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Nerve Tissue Proteins - biosynthesis</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nitric Oxide Synthase - biosynthesis</topic><topic>Nitric Oxide Synthase - genetics</topic><topic>Nitric Oxide Synthase Type I</topic><topic>Nitric Oxide Synthase Type III</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reperfusion Injury - drug therapy</topic><topic>Reperfusion Injury - prevention & control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gowda, Charan</creatorcontrib><creatorcontrib>Toomayan, Glen A</creatorcontrib><creatorcontrib>Qi, Wen-Ning</creatorcontrib><creatorcontrib>Chen, Long-En</creatorcontrib><creatorcontrib>Cai, Yongting</creatorcontrib><creatorcontrib>Allen, Diane M</creatorcontrib><creatorcontrib>Seaber, Anthony V</creatorcontrib><creatorcontrib>Urbaniak, James R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Nitric oxide</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gowda, Charan</au><au>Toomayan, Glen A</au><au>Qi, Wen-Ning</au><au>Chen, Long-En</au><au>Cai, Yongting</au><au>Allen, Diane M</au><au>Seaber, Anthony V</au><au>Urbaniak, James R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effects of N(omega)-propyl-L-arginine on reperfusion injury of skeletal muscle</atitle><jtitle>Nitric oxide</jtitle><addtitle>Nitric Oxide</addtitle><date>2004-08</date><risdate>2004</risdate><volume>11</volume><issue>1</issue><spage>17</spage><pages>17-</pages><issn>1089-8603</issn><abstract>N(omega)-Propyl-L-arginine (NPA) is reported to be a highly selective inhibitor of neuronal nitric oxide synthase (nNOS). This in vivo study observed its role in ischemia/reperfusion (I/R) injury in rat skeletal muscle. Our results showed that NPA infusion significantly increased vessel diameters and blood flow in reperfused cremaster muscle, and slightly increased contractile function in reperfused extensor digitorum longus (EDL) muscle. In addition, NPA treatment slightly increased I/R-mediated downregulation of nNOS and eNOS mRNA and protein levels. Although NPA showed a beneficial role in I/R injury, our in vivo data do not support NPA as a selective nNOS inhibitor. Also, our data do not provide any insight into the mechanism of NPA. Thus, the in vivo mechanism of action of NPA needs to be further identified, and the role of nNOS in skeletal muscle I/R still remains to be determined.</abstract><cop>United States</cop><pmid>15350553</pmid><doi>10.1016/j.niox.2004.07.007</doi></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Arginine - administration & dosage Arginine - analogs & derivatives Arginine - pharmacology Arginine - therapeutic use Blood Pressure - drug effects Drug Evaluation, Preclinical Enzyme Induction - drug effects Enzyme Inhibitors - pharmacology Female Infusions, Intravenous Injections, Intra-Arterial Ischemia - drug therapy Ischemia - metabolism Ischemia - pathology Male Microcirculation - drug effects Muscle Contraction - drug effects Muscle, Skeletal - blood supply Muscle, Skeletal - drug effects Muscle, Skeletal - metabolism Nerve Tissue Proteins - biosynthesis Nerve Tissue Proteins - genetics Nitric Oxide Synthase - biosynthesis Nitric Oxide Synthase - genetics Nitric Oxide Synthase Type I Nitric Oxide Synthase Type III Rats Rats, Sprague-Dawley Reperfusion Injury - drug therapy Reperfusion Injury - prevention & control |
| title | The effects of N(omega)-propyl-L-arginine on reperfusion injury of skeletal muscle |
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