A Rapid Aflatoxin B1 ELISA: Development and Validation with Reduced Matrix Effects for Peanuts, Corn, Pistachio, and Soybeans

Among the competitive ELISAs for aflatoxins that have been described, few have been adequately validated for reduced matrix effects. Using an aflatoxin B1 (AFB1)-specific polyclonal antibody (produced from AFB1−oxime conjugated to bovine serum albumin (BSA)) and AFB1− and AFB2−enzyme conjugates, fou...

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Veröffentlicht in:	Journal of agricultural and food chemistry 2004-05, Vol.52 (10), p.2746-2755
Hauptverfasser:	Lee, Nanju A, Wang, Shuo, Allan, Robin D, Kennedy, Ivan R
Format:	Artikel
Sprache:	eng
Schlagworte:	aflatoxin B1 Aflatoxin B1 - analysis aflatoxins Arachis - chemistry Biological and medical sciences corn cross reaction direct competitive enzyme-linked immunosorbent assay enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods food contamination Food Contamination - analysis Food industries Food toxicology Fundamental and applied biological sciences. Psychology General aspects Glycine max - chemistry Haptens Hygiene and safety microbial contamination peanuts pistachios Pistacia - chemistry rapid methods Reproducibility of Results Sensitivity and Specificity soybeans Zea mays - chemistry
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