Reactive oxygen species are involved in the activation of cellular phospholipase A2
Vanadate (V) potentiated (4‐ to 10‐fold) the activation of cellular phospholipase A2 (PLA2) induced by H2O2 (H), a phorbol ester (T), a Ca2+‐ionophore (A) and opsonized zymosan in macrophages. V+H induced in intact cells the activation and translocation of PLA2 and protein kinase C(PKC) to the plasm...
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Veröffentlicht in: | FEBS letters 1992-09, Vol.309 (2), p.190-192 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Vanadate (V) potentiated (4‐ to 10‐fold) the activation of cellular phospholipase A2 (PLA2) induced by H2O2 (H), a phorbol ester (T), a Ca2+‐ionophore (A) and opsonized zymosan in macrophages. V+H induced in intact cells the activation and translocation of PLA2 and protein kinase C(PKC) to the plasma membrane. V+H and V+T+A induced strong chemiluminescence (CL) which was abrogated by a specific NADPH oxidase inhibitor diphenylene iodonium (DPI). DPI markedly suppressed the stimulation of PLA2 by V+T+A and V+OZ. The results suggest that the formation of endogenous reactive oxygen species (ROS) is important for PLA2 activation. |
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ISSN: | 0014-5793 1873-3468 |
DOI: | 10.1016/0014-5793(92)81092-Z |