Role of cyclooxygenase in ventricular effects of adrenomedullin: is adrenomedullin a double-edged sword in sepsis?
Department of Physiology, Institute of Cardiovascular Sciences and Medicine, University of Hong Kong, Hong Kong SAR, China Submitted 10 April 2003 ; accepted in final form 22 October 2003 Adrenomedullin (ADM) is upregulated in cardiac tissue under various pathophysiological conditions. However, the...
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| Veröffentlicht in: | American journal of physiology. Heart and circulatory physiology 2004-03, Vol.286 (3), p.H1034-H1042 |
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| creator | Mittra, Shivani Hyvelin, Jean-Marc Shan, Qixian Tang, Fai Bourreau, Jean-Pierre |
| description | Department of Physiology, Institute of Cardiovascular Sciences and Medicine, University of Hong Kong, Hong Kong SAR, China
Submitted 10 April 2003
; accepted in final form 22 October 2003
Adrenomedullin (ADM) is upregulated in cardiac tissue under various pathophysiological conditions. However, the direct inotropic effect of ADM on normal and compromised cardiomyocytes is not clear. In rat ventricular myocytes, ADM produced an initial (1 h), a marked decrease in cell shortening and Ca 2+ transient. Both effects were sensitive to inhibition by the ADM antagonist ADM-(2252). The increase and decrease in cell shortening and Ca 2+ transient were attenuated by pretreatment with indomethacin [a nonspecific cyclooxygenase (COX) inhibitor], nimesulide and SC-236 (specific COX-2 inhibitors), and tranylcypromine (a prostacyclin synthase inhibitor); SQ-29548 (a thromboxane receptor antagonist) was without effect. Cells isolated from LPS-treated rats that were in the late, hypodynamic phase of septic shock also showed a marked decrease in cell shortening and Ca 2+ transient. Because ADM is overexpressed in sepsis, we repeated the above protocol in cells isolated from LPS-treated rats. At 4 h after LPS injection, ADM levels markedly increased in plasma, ventricles, and freshly isolated ventricular myocytes. Decreases in cell shortening and Ca 2+ transient in LPS-treated cells were reversed by pretreatment with ADM-(2252). Anti-ADM (rat) IgG also reversed the decrease in cell shortening and other parameters of cell kinetics. Indomethacin, SC-236, and tranylcypromine restored cell contractility and the decrease in Ca 2+ transient, whereas SQ-29548 had no effect, implying that prostacyclin played a role in both effects. However, with regard to cell-shortening kinetics, indomethacin and SQ-29548 decreased the amount of time taken by the cells to return to baseline, whereas SC-236 and tranylcypromine did not, implying that not only prostacyclin, but also thromboxane, is involved. The results indicate that ADM interacts with COX to yield prostanoids, which mediate its negative inotropic effect in LPS-treated rat ventricular myocytes.
cell shortening; calcium transient; cyclooxygenase-2; prostacyclin
Address for reprint requests and other correspondence: J.-P. Bourreau, Dept. of Physiology, 4/F, Laboratory Block, Faculty of Medicine Bldg., Univ. of Hong Kong, 21 Sassoon Rd., Hong Kong, S |
| doi_str_mv | 10.1152/ajpheart.00337.2003 |
| format | Article |
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Submitted 10 April 2003
; accepted in final form 22 October 2003
Adrenomedullin (ADM) is upregulated in cardiac tissue under various pathophysiological conditions. However, the direct inotropic effect of ADM on normal and compromised cardiomyocytes is not clear. In rat ventricular myocytes, ADM produced an initial (<30 min) increase in cell shortening and Ca 2+ transient and, on prolonged incubation (>1 h), a marked decrease in cell shortening and Ca 2+ transient. Both effects were sensitive to inhibition by the ADM antagonist ADM-(2252). The increase and decrease in cell shortening and Ca 2+ transient were attenuated by pretreatment with indomethacin [a nonspecific cyclooxygenase (COX) inhibitor], nimesulide and SC-236 (specific COX-2 inhibitors), and tranylcypromine (a prostacyclin synthase inhibitor); SQ-29548 (a thromboxane receptor antagonist) was without effect. Cells isolated from LPS-treated rats that were in the late, hypodynamic phase of septic shock also showed a marked decrease in cell shortening and Ca 2+ transient. Because ADM is overexpressed in sepsis, we repeated the above protocol in cells isolated from LPS-treated rats. At 4 h after LPS injection, ADM levels markedly increased in plasma, ventricles, and freshly isolated ventricular myocytes. Decreases in cell shortening and Ca 2+ transient in LPS-treated cells were reversed by pretreatment with ADM-(2252). Anti-ADM (rat) IgG also reversed the decrease in cell shortening and other parameters of cell kinetics. Indomethacin, SC-236, and tranylcypromine restored cell contractility and the decrease in Ca 2+ transient, whereas SQ-29548 had no effect, implying that prostacyclin played a role in both effects. However, with regard to cell-shortening kinetics, indomethacin and SQ-29548 decreased the amount of time taken by the cells to return to baseline, whereas SC-236 and tranylcypromine did not, implying that not only prostacyclin, but also thromboxane, is involved. The results indicate that ADM interacts with COX to yield prostanoids, which mediate its negative inotropic effect in LPS-treated rat ventricular myocytes.
cell shortening; calcium transient; cyclooxygenase-2; prostacyclin
Address for reprint requests and other correspondence: J.-P. Bourreau, Dept. of Physiology, 4/F, Laboratory Block, Faculty of Medicine Bldg., Univ. of Hong Kong, 21 Sassoon Rd., Hong Kong, SAR, China (E-mail: bourreau{at}hkucc.hku.hk ).</description><identifier>ISSN: 0363-6135</identifier><identifier>EISSN: 1522-1539</identifier><identifier>DOI: 10.1152/ajpheart.00337.2003</identifier><identifier>PMID: 14766677</identifier><language>eng</language><publisher>United States</publisher><subject>Adrenomedullin ; Anesthesia ; Animals ; Blood Pressure ; Calcium Signaling - drug effects ; Calcium Signaling - physiology ; Cyclooxygenase 1 ; Cyclooxygenase 2 ; Isoenzymes - metabolism ; Lipopolysaccharides - pharmacology ; Male ; Membrane Proteins ; Myocardial Contraction - drug effects ; Myocytes, Cardiac - metabolism ; Peptide Fragments - pharmacology ; Peptides - metabolism ; Prostaglandin-Endoperoxide Synthases - metabolism ; Radioimmunoassay ; Rats ; Rats, Sprague-Dawley ; Sepsis - metabolism</subject><ispartof>American journal of physiology. Heart and circulatory physiology, 2004-03, Vol.286 (3), p.H1034-H1042</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-d1baa7ae4edbed96a2e7e5a5c09e7a77708e187089b0bd72d41beac4a19e5d8b3</citedby><cites>FETCH-LOGICAL-c393t-d1baa7ae4edbed96a2e7e5a5c09e7a77708e187089b0bd72d41beac4a19e5d8b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3039,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14766677$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mittra, Shivani</creatorcontrib><creatorcontrib>Hyvelin, Jean-Marc</creatorcontrib><creatorcontrib>Shan, Qixian</creatorcontrib><creatorcontrib>Tang, Fai</creatorcontrib><creatorcontrib>Bourreau, Jean-Pierre</creatorcontrib><title>Role of cyclooxygenase in ventricular effects of adrenomedullin: is adrenomedullin a double-edged sword in sepsis?</title><title>American journal of physiology. Heart and circulatory physiology</title><addtitle>Am J Physiol Heart Circ Physiol</addtitle><description>Department of Physiology, Institute of Cardiovascular Sciences and Medicine, University of Hong Kong, Hong Kong SAR, China
Submitted 10 April 2003
; accepted in final form 22 October 2003
Adrenomedullin (ADM) is upregulated in cardiac tissue under various pathophysiological conditions. However, the direct inotropic effect of ADM on normal and compromised cardiomyocytes is not clear. In rat ventricular myocytes, ADM produced an initial (<30 min) increase in cell shortening and Ca 2+ transient and, on prolonged incubation (>1 h), a marked decrease in cell shortening and Ca 2+ transient. Both effects were sensitive to inhibition by the ADM antagonist ADM-(2252). The increase and decrease in cell shortening and Ca 2+ transient were attenuated by pretreatment with indomethacin [a nonspecific cyclooxygenase (COX) inhibitor], nimesulide and SC-236 (specific COX-2 inhibitors), and tranylcypromine (a prostacyclin synthase inhibitor); SQ-29548 (a thromboxane receptor antagonist) was without effect. Cells isolated from LPS-treated rats that were in the late, hypodynamic phase of septic shock also showed a marked decrease in cell shortening and Ca 2+ transient. Because ADM is overexpressed in sepsis, we repeated the above protocol in cells isolated from LPS-treated rats. At 4 h after LPS injection, ADM levels markedly increased in plasma, ventricles, and freshly isolated ventricular myocytes. Decreases in cell shortening and Ca 2+ transient in LPS-treated cells were reversed by pretreatment with ADM-(2252). Anti-ADM (rat) IgG also reversed the decrease in cell shortening and other parameters of cell kinetics. Indomethacin, SC-236, and tranylcypromine restored cell contractility and the decrease in Ca 2+ transient, whereas SQ-29548 had no effect, implying that prostacyclin played a role in both effects. However, with regard to cell-shortening kinetics, indomethacin and SQ-29548 decreased the amount of time taken by the cells to return to baseline, whereas SC-236 and tranylcypromine did not, implying that not only prostacyclin, but also thromboxane, is involved. The results indicate that ADM interacts with COX to yield prostanoids, which mediate its negative inotropic effect in LPS-treated rat ventricular myocytes.
cell shortening; calcium transient; cyclooxygenase-2; prostacyclin
Address for reprint requests and other correspondence: J.-P. Bourreau, Dept. of Physiology, 4/F, Laboratory Block, Faculty of Medicine Bldg., Univ. of Hong Kong, 21 Sassoon Rd., Hong Kong, SAR, China (E-mail: bourreau{at}hkucc.hku.hk ).</description><subject>Adrenomedullin</subject><subject>Anesthesia</subject><subject>Animals</subject><subject>Blood Pressure</subject><subject>Calcium Signaling - drug effects</subject><subject>Calcium Signaling - physiology</subject><subject>Cyclooxygenase 1</subject><subject>Cyclooxygenase 2</subject><subject>Isoenzymes - metabolism</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Male</subject><subject>Membrane Proteins</subject><subject>Myocardial Contraction - drug effects</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>Peptide Fragments - pharmacology</subject><subject>Peptides - metabolism</subject><subject>Prostaglandin-Endoperoxide Synthases - metabolism</subject><subject>Radioimmunoassay</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Sepsis - metabolism</subject><issn>0363-6135</issn><issn>1522-1539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kNFqFDEUhoModq0-gSC58m62yWQmmdELkWJboSBIvQ5nkjO7KdnJmMy0nbc3626rCN7kQPL9PycfIW85W3Nel2dwO24R4rRmTAi1LvN4Rlb5pSx4LdrnZMWEFIXkoj4hr1K6ZYzVSoqX5IRXSkqp1IrE78EjDT01i_EhPCwbHCAhdQO9w2GKzsweIsW-RzOlPQg24hB2aGfv3fCBuvTPFQVqw9x5LNBu0NJ0H6LdFyYck0ufXpMXPfiEb47zlPy4-HJzflVcf7v8ev75ujCiFVNheQegACu0HdpWQokKa6gNa1GBUoo1yJt8th3rrCptxTsEUwFvsbZNJ07J-0PvGMPPGdOkdy4Z9B4GDHPSDeNV03KZQXEATQwpRez1GN0O4qI503vV-lG1_q1a71Xn1Ltj_dzlr__JHN1m4OMB2LrN9t5F1ON2SS74sFn0RVZ1gw_TU3XZSC30FWei0qPtc_rs_-mnff5KiV-FLKYX</recordid><startdate>20040301</startdate><enddate>20040301</enddate><creator>Mittra, Shivani</creator><creator>Hyvelin, Jean-Marc</creator><creator>Shan, Qixian</creator><creator>Tang, Fai</creator><creator>Bourreau, Jean-Pierre</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040301</creationdate><title>Role of cyclooxygenase in ventricular effects of adrenomedullin: is adrenomedullin a double-edged sword in sepsis?</title><author>Mittra, Shivani ; Hyvelin, Jean-Marc ; Shan, Qixian ; Tang, Fai ; Bourreau, Jean-Pierre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-d1baa7ae4edbed96a2e7e5a5c09e7a77708e187089b0bd72d41beac4a19e5d8b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adrenomedullin</topic><topic>Anesthesia</topic><topic>Animals</topic><topic>Blood Pressure</topic><topic>Calcium Signaling - drug effects</topic><topic>Calcium Signaling - physiology</topic><topic>Cyclooxygenase 1</topic><topic>Cyclooxygenase 2</topic><topic>Isoenzymes - metabolism</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Male</topic><topic>Membrane Proteins</topic><topic>Myocardial Contraction - drug effects</topic><topic>Myocytes, Cardiac - metabolism</topic><topic>Peptide Fragments - pharmacology</topic><topic>Peptides - metabolism</topic><topic>Prostaglandin-Endoperoxide Synthases - metabolism</topic><topic>Radioimmunoassay</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Sepsis - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mittra, Shivani</creatorcontrib><creatorcontrib>Hyvelin, Jean-Marc</creatorcontrib><creatorcontrib>Shan, Qixian</creatorcontrib><creatorcontrib>Tang, Fai</creatorcontrib><creatorcontrib>Bourreau, Jean-Pierre</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. Heart and circulatory physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mittra, Shivani</au><au>Hyvelin, Jean-Marc</au><au>Shan, Qixian</au><au>Tang, Fai</au><au>Bourreau, Jean-Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of cyclooxygenase in ventricular effects of adrenomedullin: is adrenomedullin a double-edged sword in sepsis?</atitle><jtitle>American journal of physiology. Heart and circulatory physiology</jtitle><addtitle>Am J Physiol Heart Circ Physiol</addtitle><date>2004-03-01</date><risdate>2004</risdate><volume>286</volume><issue>3</issue><spage>H1034</spage><epage>H1042</epage><pages>H1034-H1042</pages><issn>0363-6135</issn><eissn>1522-1539</eissn><abstract>Department of Physiology, Institute of Cardiovascular Sciences and Medicine, University of Hong Kong, Hong Kong SAR, China
Submitted 10 April 2003
; accepted in final form 22 October 2003
Adrenomedullin (ADM) is upregulated in cardiac tissue under various pathophysiological conditions. However, the direct inotropic effect of ADM on normal and compromised cardiomyocytes is not clear. In rat ventricular myocytes, ADM produced an initial (<30 min) increase in cell shortening and Ca 2+ transient and, on prolonged incubation (>1 h), a marked decrease in cell shortening and Ca 2+ transient. Both effects were sensitive to inhibition by the ADM antagonist ADM-(2252). The increase and decrease in cell shortening and Ca 2+ transient were attenuated by pretreatment with indomethacin [a nonspecific cyclooxygenase (COX) inhibitor], nimesulide and SC-236 (specific COX-2 inhibitors), and tranylcypromine (a prostacyclin synthase inhibitor); SQ-29548 (a thromboxane receptor antagonist) was without effect. Cells isolated from LPS-treated rats that were in the late, hypodynamic phase of septic shock also showed a marked decrease in cell shortening and Ca 2+ transient. Because ADM is overexpressed in sepsis, we repeated the above protocol in cells isolated from LPS-treated rats. At 4 h after LPS injection, ADM levels markedly increased in plasma, ventricles, and freshly isolated ventricular myocytes. Decreases in cell shortening and Ca 2+ transient in LPS-treated cells were reversed by pretreatment with ADM-(2252). Anti-ADM (rat) IgG also reversed the decrease in cell shortening and other parameters of cell kinetics. Indomethacin, SC-236, and tranylcypromine restored cell contractility and the decrease in Ca 2+ transient, whereas SQ-29548 had no effect, implying that prostacyclin played a role in both effects. However, with regard to cell-shortening kinetics, indomethacin and SQ-29548 decreased the amount of time taken by the cells to return to baseline, whereas SC-236 and tranylcypromine did not, implying that not only prostacyclin, but also thromboxane, is involved. The results indicate that ADM interacts with COX to yield prostanoids, which mediate its negative inotropic effect in LPS-treated rat ventricular myocytes.
cell shortening; calcium transient; cyclooxygenase-2; prostacyclin
Address for reprint requests and other correspondence: J.-P. Bourreau, Dept. of Physiology, 4/F, Laboratory Block, Faculty of Medicine Bldg., Univ. of Hong Kong, 21 Sassoon Rd., Hong Kong, SAR, China (E-mail: bourreau{at}hkucc.hku.hk ).</abstract><cop>United States</cop><pmid>14766677</pmid><doi>10.1152/ajpheart.00337.2003</doi></addata></record> |
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| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adrenomedullin Anesthesia Animals Blood Pressure Calcium Signaling - drug effects Calcium Signaling - physiology Cyclooxygenase 1 Cyclooxygenase 2 Isoenzymes - metabolism Lipopolysaccharides - pharmacology Male Membrane Proteins Myocardial Contraction - drug effects Myocytes, Cardiac - metabolism Peptide Fragments - pharmacology Peptides - metabolism Prostaglandin-Endoperoxide Synthases - metabolism Radioimmunoassay Rats Rats, Sprague-Dawley Sepsis - metabolism |
| title | Role of cyclooxygenase in ventricular effects of adrenomedullin: is adrenomedullin a double-edged sword in sepsis? |
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