Gibberellin Is Essentially Required for Carrot (Daucus carota L.) Somatic Embryogenesis: Dynamic Regulation of Gibberellin 3-Oxidase Gene Expressions
A GA biosynthesis inhibitor, uniconazole, caused many shrunken embryos when it was supplied to cultured carrot (Daucus carota L.) cells at the induction of somatic embryos. The abnormality was prevented by exogenous GA 1 or GA 4 . To analyze the status of GA biosynthesis during somatic embryogenesis...
Gespeichert in:
| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003, Vol.67 (11), p.2438-2447 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2447 |
|---|---|
| container_issue | 11 |
| container_start_page | 2438 |
| container_title | Bioscience, biotechnology, and biochemistry |
| container_volume | 67 |
| creator | MITSUHASHI, Wataru TOYOMASU, Tomonobu MASUI, Hiroyuki KATHO, Toshinori NAKAMINAMI, Kentaro KASHIWAGI, Yoshiko AKUTSU, Mitsuaki KENMOKU, Hiromichi SASSA, Takeshi YAMAGUCHI, Shinjiro KAMIYA, Yuji KAMADA, Hiroshi |
| description | A GA biosynthesis inhibitor, uniconazole, caused many shrunken embryos when it was supplied to cultured carrot (Daucus carota L.) cells at the induction of somatic embryos. The abnormality was prevented by exogenous GA
1
or GA
4
. To analyze the status of GA biosynthesis during somatic embryogenesis, expression patterns of newly isolated genes encoding GA biosynthetic enzymes, two GA 20-oxidases, three GA 3-oxidases, and two GA 2-oxidases were observed by using a semi-quantitative reverse-transcription-polymerase chain reaction with gene-specific primers. Transcript levels of GA 20-oxidases and GA 2-oxidases did not change greatly during development of the somatic embryo. On the other hand, drastic changes were found in three GA 3-oxidase genes. Strikingly, expression of a GA 3-oxidase gene, DcGA3ox2, was elevated once in somatic embryogenesis, but not in the non-induced suspension cells. The enzymatic functions of these gene products were also confirmed using recombinant proteins expressed in Escherichia coli. Our results indicate that GA biosynthesis is required for carrot somatic embryogenesis. |
| doi_str_mv | 10.1271/bbb.67.2438 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_pasca</sourceid><recordid>TN_cdi_pubmed_primary_14646205</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>14646205</sourcerecordid><originalsourceid>FETCH-LOGICAL-c560t-6dbf96957c0e8e04c479e344b8e60064139121d1469d7e959e939cb3110287593</originalsourceid><addsrcrecordid>eNpt0M9rFDEUB_Agit1WT94lF8EisyaTX5PeZLuuhYVC1fOQZN6USGayJjPY-UP8f03ZlXrwFN7j816SL0JvKFnTWtGP1tq1VOuas-YZWlHGVSU1V8_Rimgqq4YLeobOc_5BSGkI-hKdUS65rIlYod87by0kCMGP-Cbjbc4wTt6EsOA7-Dn7BB3uY8Ibk1Kc8PtrM7s5Y2dKZfB-fYm_xsFM3uHtYNMS72GE7PMVvl5GM5T2HdzPoYA44tjjf69j1e2D70wGvCtDePtwSJBzgfkVetGbkOH16bxA3z9vv22-VPvb3c3m075yQpKpkp3ttdRCOQINEO640sA4tw1IQiSnTNOaduW3ulOghQbNtLOMUlI3Smh2gT4c97oUc07Qt4fkB5OWlpL2Mdy2hNtK1T6GW_Tboz7MdoDuyZ7SLODdCZjsTOiTGZ3PT05wImpKipNH58cS7WB-xRS6djJLiOnvEPvfC_4AcpuU7A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Gibberellin Is Essentially Required for Carrot (Daucus carota L.) Somatic Embryogenesis: Dynamic Regulation of Gibberellin 3-Oxidase Gene Expressions</title><source>J-STAGE Free</source><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Open Access Titles of Japan</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Free Full-Text Journals in Chemistry</source><creator>MITSUHASHI, Wataru ; TOYOMASU, Tomonobu ; MASUI, Hiroyuki ; KATHO, Toshinori ; NAKAMINAMI, Kentaro ; KASHIWAGI, Yoshiko ; AKUTSU, Mitsuaki ; KENMOKU, Hiromichi ; SASSA, Takeshi ; YAMAGUCHI, Shinjiro ; KAMIYA, Yuji ; KAMADA, Hiroshi</creator><creatorcontrib>MITSUHASHI, Wataru ; TOYOMASU, Tomonobu ; MASUI, Hiroyuki ; KATHO, Toshinori ; NAKAMINAMI, Kentaro ; KASHIWAGI, Yoshiko ; AKUTSU, Mitsuaki ; KENMOKU, Hiromichi ; SASSA, Takeshi ; YAMAGUCHI, Shinjiro ; KAMIYA, Yuji ; KAMADA, Hiroshi</creatorcontrib><description>A GA biosynthesis inhibitor, uniconazole, caused many shrunken embryos when it was supplied to cultured carrot (Daucus carota L.) cells at the induction of somatic embryos. The abnormality was prevented by exogenous GA
1
or GA
4
. To analyze the status of GA biosynthesis during somatic embryogenesis, expression patterns of newly isolated genes encoding GA biosynthetic enzymes, two GA 20-oxidases, three GA 3-oxidases, and two GA 2-oxidases were observed by using a semi-quantitative reverse-transcription-polymerase chain reaction with gene-specific primers. Transcript levels of GA 20-oxidases and GA 2-oxidases did not change greatly during development of the somatic embryo. On the other hand, drastic changes were found in three GA 3-oxidase genes. Strikingly, expression of a GA 3-oxidase gene, DcGA3ox2, was elevated once in somatic embryogenesis, but not in the non-induced suspension cells. The enzymatic functions of these gene products were also confirmed using recombinant proteins expressed in Escherichia coli. Our results indicate that GA biosynthesis is required for carrot somatic embryogenesis.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.67.2438</identifier><identifier>PMID: 14646205</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Base Sequence ; Biological and medical sciences ; carrot ; Cells, Cultured ; Cloning, Molecular ; Daucus carota - enzymology ; Daucus carota - growth & development ; DNA Primers ; DNA, Complementary - genetics ; Fundamental and applied biological sciences. Psychology ; GA biosynthesis ; Gibberellins - metabolism ; Mixed Function Oxygenases - genetics ; Mixed Function Oxygenases - metabolism ; Open Reading Frames ; Phylogeny ; Plant Growth Regulators - physiology ; Polymerase Chain Reaction ; Seeds - enzymology ; somatic embryogenesis</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2003, Vol.67 (11), p.2438-2447</ispartof><rights>2003 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2003</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c560t-6dbf96957c0e8e04c479e344b8e60064139121d1469d7e959e939cb3110287593</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15405210$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14646205$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MITSUHASHI, Wataru</creatorcontrib><creatorcontrib>TOYOMASU, Tomonobu</creatorcontrib><creatorcontrib>MASUI, Hiroyuki</creatorcontrib><creatorcontrib>KATHO, Toshinori</creatorcontrib><creatorcontrib>NAKAMINAMI, Kentaro</creatorcontrib><creatorcontrib>KASHIWAGI, Yoshiko</creatorcontrib><creatorcontrib>AKUTSU, Mitsuaki</creatorcontrib><creatorcontrib>KENMOKU, Hiromichi</creatorcontrib><creatorcontrib>SASSA, Takeshi</creatorcontrib><creatorcontrib>YAMAGUCHI, Shinjiro</creatorcontrib><creatorcontrib>KAMIYA, Yuji</creatorcontrib><creatorcontrib>KAMADA, Hiroshi</creatorcontrib><title>Gibberellin Is Essentially Required for Carrot (Daucus carota L.) Somatic Embryogenesis: Dynamic Regulation of Gibberellin 3-Oxidase Gene Expressions</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>A GA biosynthesis inhibitor, uniconazole, caused many shrunken embryos when it was supplied to cultured carrot (Daucus carota L.) cells at the induction of somatic embryos. The abnormality was prevented by exogenous GA
1
or GA
4
. To analyze the status of GA biosynthesis during somatic embryogenesis, expression patterns of newly isolated genes encoding GA biosynthetic enzymes, two GA 20-oxidases, three GA 3-oxidases, and two GA 2-oxidases were observed by using a semi-quantitative reverse-transcription-polymerase chain reaction with gene-specific primers. Transcript levels of GA 20-oxidases and GA 2-oxidases did not change greatly during development of the somatic embryo. On the other hand, drastic changes were found in three GA 3-oxidase genes. Strikingly, expression of a GA 3-oxidase gene, DcGA3ox2, was elevated once in somatic embryogenesis, but not in the non-induced suspension cells. The enzymatic functions of these gene products were also confirmed using recombinant proteins expressed in Escherichia coli. Our results indicate that GA biosynthesis is required for carrot somatic embryogenesis.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>carrot</subject><subject>Cells, Cultured</subject><subject>Cloning, Molecular</subject><subject>Daucus carota - enzymology</subject><subject>Daucus carota - growth & development</subject><subject>DNA Primers</subject><subject>DNA, Complementary - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GA biosynthesis</subject><subject>Gibberellins - metabolism</subject><subject>Mixed Function Oxygenases - genetics</subject><subject>Mixed Function Oxygenases - metabolism</subject><subject>Open Reading Frames</subject><subject>Phylogeny</subject><subject>Plant Growth Regulators - physiology</subject><subject>Polymerase Chain Reaction</subject><subject>Seeds - enzymology</subject><subject>somatic embryogenesis</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0M9rFDEUB_Agit1WT94lF8EisyaTX5PeZLuuhYVC1fOQZN6USGayJjPY-UP8f03ZlXrwFN7j816SL0JvKFnTWtGP1tq1VOuas-YZWlHGVSU1V8_Rimgqq4YLeobOc_5BSGkI-hKdUS65rIlYod87by0kCMGP-Cbjbc4wTt6EsOA7-Dn7BB3uY8Ibk1Kc8PtrM7s5Y2dKZfB-fYm_xsFM3uHtYNMS72GE7PMVvl5GM5T2HdzPoYA44tjjf69j1e2D70wGvCtDePtwSJBzgfkVetGbkOH16bxA3z9vv22-VPvb3c3m075yQpKpkp3ttdRCOQINEO640sA4tw1IQiSnTNOaduW3ulOghQbNtLOMUlI3Smh2gT4c97oUc07Qt4fkB5OWlpL2Mdy2hNtK1T6GW_Tboz7MdoDuyZ7SLODdCZjsTOiTGZ3PT05wImpKipNH58cS7WB-xRS6djJLiOnvEPvfC_4AcpuU7A</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>MITSUHASHI, Wataru</creator><creator>TOYOMASU, Tomonobu</creator><creator>MASUI, Hiroyuki</creator><creator>KATHO, Toshinori</creator><creator>NAKAMINAMI, Kentaro</creator><creator>KASHIWAGI, Yoshiko</creator><creator>AKUTSU, Mitsuaki</creator><creator>KENMOKU, Hiromichi</creator><creator>SASSA, Takeshi</creator><creator>YAMAGUCHI, Shinjiro</creator><creator>KAMIYA, Yuji</creator><creator>KAMADA, Hiroshi</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2003</creationdate><title>Gibberellin Is Essentially Required for Carrot (Daucus carota L.) Somatic Embryogenesis: Dynamic Regulation of Gibberellin 3-Oxidase Gene Expressions</title><author>MITSUHASHI, Wataru ; TOYOMASU, Tomonobu ; MASUI, Hiroyuki ; KATHO, Toshinori ; NAKAMINAMI, Kentaro ; KASHIWAGI, Yoshiko ; AKUTSU, Mitsuaki ; KENMOKU, Hiromichi ; SASSA, Takeshi ; YAMAGUCHI, Shinjiro ; KAMIYA, Yuji ; KAMADA, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c560t-6dbf96957c0e8e04c479e344b8e60064139121d1469d7e959e939cb3110287593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>carrot</topic><topic>Cells, Cultured</topic><topic>Cloning, Molecular</topic><topic>Daucus carota - enzymology</topic><topic>Daucus carota - growth & development</topic><topic>DNA Primers</topic><topic>DNA, Complementary - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GA biosynthesis</topic><topic>Gibberellins - metabolism</topic><topic>Mixed Function Oxygenases - genetics</topic><topic>Mixed Function Oxygenases - metabolism</topic><topic>Open Reading Frames</topic><topic>Phylogeny</topic><topic>Plant Growth Regulators - physiology</topic><topic>Polymerase Chain Reaction</topic><topic>Seeds - enzymology</topic><topic>somatic embryogenesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MITSUHASHI, Wataru</creatorcontrib><creatorcontrib>TOYOMASU, Tomonobu</creatorcontrib><creatorcontrib>MASUI, Hiroyuki</creatorcontrib><creatorcontrib>KATHO, Toshinori</creatorcontrib><creatorcontrib>NAKAMINAMI, Kentaro</creatorcontrib><creatorcontrib>KASHIWAGI, Yoshiko</creatorcontrib><creatorcontrib>AKUTSU, Mitsuaki</creatorcontrib><creatorcontrib>KENMOKU, Hiromichi</creatorcontrib><creatorcontrib>SASSA, Takeshi</creatorcontrib><creatorcontrib>YAMAGUCHI, Shinjiro</creatorcontrib><creatorcontrib>KAMIYA, Yuji</creatorcontrib><creatorcontrib>KAMADA, Hiroshi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MITSUHASHI, Wataru</au><au>TOYOMASU, Tomonobu</au><au>MASUI, Hiroyuki</au><au>KATHO, Toshinori</au><au>NAKAMINAMI, Kentaro</au><au>KASHIWAGI, Yoshiko</au><au>AKUTSU, Mitsuaki</au><au>KENMOKU, Hiromichi</au><au>SASSA, Takeshi</au><au>YAMAGUCHI, Shinjiro</au><au>KAMIYA, Yuji</au><au>KAMADA, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gibberellin Is Essentially Required for Carrot (Daucus carota L.) Somatic Embryogenesis: Dynamic Regulation of Gibberellin 3-Oxidase Gene Expressions</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2003</date><risdate>2003</risdate><volume>67</volume><issue>11</issue><spage>2438</spage><epage>2447</epage><pages>2438-2447</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>A GA biosynthesis inhibitor, uniconazole, caused many shrunken embryos when it was supplied to cultured carrot (Daucus carota L.) cells at the induction of somatic embryos. The abnormality was prevented by exogenous GA
1
or GA
4
. To analyze the status of GA biosynthesis during somatic embryogenesis, expression patterns of newly isolated genes encoding GA biosynthetic enzymes, two GA 20-oxidases, three GA 3-oxidases, and two GA 2-oxidases were observed by using a semi-quantitative reverse-transcription-polymerase chain reaction with gene-specific primers. Transcript levels of GA 20-oxidases and GA 2-oxidases did not change greatly during development of the somatic embryo. On the other hand, drastic changes were found in three GA 3-oxidase genes. Strikingly, expression of a GA 3-oxidase gene, DcGA3ox2, was elevated once in somatic embryogenesis, but not in the non-induced suspension cells. The enzymatic functions of these gene products were also confirmed using recombinant proteins expressed in Escherichia coli. Our results indicate that GA biosynthesis is required for carrot somatic embryogenesis.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>14646205</pmid><doi>10.1271/bbb.67.2438</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 2003, Vol.67 (11), p.2438-2447 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_pubmed_primary_14646205 |
| source | J-STAGE Free; MEDLINE; Oxford University Press Journals All Titles (1996-Current); Open Access Titles of Japan; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Base Sequence Biological and medical sciences carrot Cells, Cultured Cloning, Molecular Daucus carota - enzymology Daucus carota - growth & development DNA Primers DNA, Complementary - genetics Fundamental and applied biological sciences. Psychology GA biosynthesis Gibberellins - metabolism Mixed Function Oxygenases - genetics Mixed Function Oxygenases - metabolism Open Reading Frames Phylogeny Plant Growth Regulators - physiology Polymerase Chain Reaction Seeds - enzymology somatic embryogenesis |
| title | Gibberellin Is Essentially Required for Carrot (Daucus carota L.) Somatic Embryogenesis: Dynamic Regulation of Gibberellin 3-Oxidase Gene Expressions |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T13%3A21%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Gibberellin%20Is%20Essentially%20Required%20for%20Carrot%20(Daucus%20carota%20L.)%20Somatic%20Embryogenesis:%20Dynamic%20Regulation%20of%20Gibberellin%203-Oxidase%20Gene%20Expressions&rft.jtitle=Bioscience,%20biotechnology,%20and%20biochemistry&rft.au=MITSUHASHI,%20Wataru&rft.date=2003&rft.volume=67&rft.issue=11&rft.spage=2438&rft.epage=2447&rft.pages=2438-2447&rft.issn=0916-8451&rft.eissn=1347-6947&rft_id=info:doi/10.1271/bbb.67.2438&rft_dat=%3Cpubmed_pasca%3E14646205%3C/pubmed_pasca%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/14646205&rfr_iscdi=true |