3M Petrifilm staph express count plate method for the enumeration of Staphylococcus aureus in selected types of processed and prepared foods: collaborative study

The 3M Petrifilm Staph Express Count plate method was compared with AOAC Official Method 975.55 for the enumeration of Staphylococcus aureus in selected foods. Five foods--frozen lasagna, custard, frozen mixed vegetables, frozen hashbrowns, and frozen batter-coated mushrooms--were analyzed for S. au...

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Veröffentlicht in:Journal of AOAC International 2003-09, Vol.86 (5), p.954-962
Hauptverfasser: Silbernagel, K.M, Jechorek, R.P, Carver, C.N, Horter, B.L, Lindberg, K.G
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container_end_page 962
container_issue 5
container_start_page 954
container_title Journal of AOAC International
container_volume 86
creator Silbernagel, K.M
Jechorek, R.P
Carver, C.N
Horter, B.L
Lindberg, K.G
description The 3M Petrifilm Staph Express Count plate method was compared with AOAC Official Method 975.55 for the enumeration of Staphylococcus aureus in selected foods. Five foods--frozen lasagna, custard, frozen mixed vegetables, frozen hashbrowns, and frozen batter-coated mushrooms--were analyzed for S. aureus by 13 collaborating laboratories. For each food tested, the collaborators received 8 blind test samples consisting of a control sample, a low inoculation level, a medium inoculation level, and a medium inoculation level with background flora, each in duplicate. The mean log10 counts for the methods were comparable for all 5 foods. The repeatability and reproducibility variances of the 24 h Petrifilm Staph Express Count plate method were similar to those of the 72 h standard method.
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Five foods--frozen lasagna, custard, frozen mixed vegetables, frozen hashbrowns, and frozen batter-coated mushrooms--were analyzed for S. aureus by 13 collaborating laboratories. For each food tested, the collaborators received 8 blind test samples consisting of a control sample, a low inoculation level, a medium inoculation level, and a medium inoculation level with background flora, each in duplicate. The mean log10 counts for the methods were comparable for all 5 foods. The repeatability and reproducibility variances of the 24 h Petrifilm Staph Express Count plate method were similar to those of the 72 h standard method.</abstract><cop>Gaithersburg, MD</cop><pub>AOAC International</pub><pmid>14632397</pmid><tpages>9</tpages></addata></record>
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source Oxford University Press Journals All Titles (1996-Current); MEDLINE
subjects Agaricales
bacterial contamination
Biological and medical sciences
Colony Count, Microbial - instrumentation
Colony Count, Microbial - methods
custards
Food Handling
Food industries
Food Microbiology
Food Preservation - methods
Freezing
frozen batter coated mushrooms
frozen foods
frozen hashbrown potatoes
frozen lasagna
frozen vegetables
Fundamental and applied biological sciences. Psychology
General aspects
Methods of analysis, processing and quality control, regulation, standards
mushrooms
pasta
plate count
potato products
Reproducibility of Results
Staphylococcus aureus
Staphylococcus aureus - isolation & purification
Vegetables - microbiology
title 3M Petrifilm staph express count plate method for the enumeration of Staphylococcus aureus in selected types of processed and prepared foods: collaborative study
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