S100C/A11 is a key mediator of Ca(2+)-induced growth inhibition of human epidermal keratinocytes
An increase in extracellular Ca2+ induces growth arrest and differentiation of human keratinocytes in culture. We examined possible involvement of S100C/A11 in this growth regulation. On exposure of the cells to high Ca2+, S100C/A11 was specifically phosphorylated at 10Thr and 94Ser. Phosphorylation...
Gespeichert in:
| Veröffentlicht in: | The Journal of cell biology 2003-11, Vol.163 (4), p.825 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 4 |
| container_start_page | 825 |
| container_title | The Journal of cell biology |
| container_volume | 163 |
| creator | Sakaguchi, Masakiyo Miyazaki, Masahiro Takaishi, Mikiro Sakaguchi, Yoshihiko Makino, Eiichi Kataoka, Noriyuki Yamada, Hidenori Namba, Masayoshi Huh, Nam-ho |
| description | An increase in extracellular Ca2+ induces growth arrest and differentiation of human keratinocytes in culture. We examined possible involvement of S100C/A11 in this growth regulation. On exposure of the cells to high Ca2+, S100C/A11 was specifically phosphorylated at 10Thr and 94Ser. Phosphorylation facilitated the binding of S100C/A11 to nucleolin, resulting in nuclear translocation of S100C/A11. In nuclei, S100C/A11 liberated Sp1/3 from nucleolin. The resulting free Sp1/3 transcriptionally activated p21CIP1/WAF1, a representative negative regulator of cell growth. Introduction of anti-S100C/A11 antibody into the cells largely abolished the growth inhibition induced by Ca2+ and the induction of p21CIP1/WAF1. In the human epidermis, S100C/A11 was detected in nuclei of differentiating cells in the suprabasal layers, but not in nuclei of proliferating cells in the basal layer. These results indicate that S100C/A11 is a key mediator of the Ca(2+)-induced growth inhibition of human keratinocytes in culture, and that it may be possibly involved in the growth regulation in vivo as well. |
| doi_str_mv | 10.1083/jcb.200304017 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed</sourceid><recordid>TN_cdi_pubmed_primary_14623863</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>14623863</sourcerecordid><originalsourceid>FETCH-LOGICAL-p122t-4fca21a71862c7de6e3c63426db42486f8684b0f43aee75bf9a0fb1cae30b2583</originalsourceid><addsrcrecordid>eNo1jztPwzAYAD2AaCmMrMgjCKX9_IjjjlVUHlIlBmAunx2buDQPOa5Q_j1FwHTL6aQj5IrBnIEWi501cw4gQAIrTsgUgLNsmfN8Qs6HYQcAspDijEyYVFxoJabk_YUBlIsVYzQMFOmnG2njqoCpi7TztMQbfnebhbY6WFfRj9h9pZqGtg4mpNC1P059aLClrg-Viw3uj42IKbSdHZMbLsipx_3gLv84I2_369fyMds8PzyVq03WM85TJr1FzrBgWnFbVE45YZWQXFVGcqmV10pLA14KdK7IjV8ieMMsOgGG51rMyPVvtz-Y48C2j6HBOG7_V8U3doZSwg</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>S100C/A11 is a key mediator of Ca(2+)-induced growth inhibition of human epidermal keratinocytes</title><source>MEDLINE</source><source>Free E-Journal (出版社公開部分のみ）</source><source>Alma/SFX Local Collection</source><creator>Sakaguchi, Masakiyo ; Miyazaki, Masahiro ; Takaishi, Mikiro ; Sakaguchi, Yoshihiko ; Makino, Eiichi ; Kataoka, Noriyuki ; Yamada, Hidenori ; Namba, Masayoshi ; Huh, Nam-ho</creator><creatorcontrib>Sakaguchi, Masakiyo ; Miyazaki, Masahiro ; Takaishi, Mikiro ; Sakaguchi, Yoshihiko ; Makino, Eiichi ; Kataoka, Noriyuki ; Yamada, Hidenori ; Namba, Masayoshi ; Huh, Nam-ho</creatorcontrib><description>An increase in extracellular Ca2+ induces growth arrest and differentiation of human keratinocytes in culture. We examined possible involvement of S100C/A11 in this growth regulation. On exposure of the cells to high Ca2+, S100C/A11 was specifically phosphorylated at 10Thr and 94Ser. Phosphorylation facilitated the binding of S100C/A11 to nucleolin, resulting in nuclear translocation of S100C/A11. In nuclei, S100C/A11 liberated Sp1/3 from nucleolin. The resulting free Sp1/3 transcriptionally activated p21CIP1/WAF1, a representative negative regulator of cell growth. Introduction of anti-S100C/A11 antibody into the cells largely abolished the growth inhibition induced by Ca2+ and the induction of p21CIP1/WAF1. In the human epidermis, S100C/A11 was detected in nuclei of differentiating cells in the suprabasal layers, but not in nuclei of proliferating cells in the basal layer. These results indicate that S100C/A11 is a key mediator of the Ca(2+)-induced growth inhibition of human keratinocytes in culture, and that it may be possibly involved in the growth regulation in vivo as well.</description><identifier>ISSN: 0021-9525</identifier><identifier>DOI: 10.1083/jcb.200304017</identifier><identifier>PMID: 14623863</identifier><language>eng</language><publisher>United States</publisher><subject>Active Transport, Cell Nucleus - drug effects ; Active Transport, Cell Nucleus - physiology ; Animals ; Antibodies - pharmacology ; Binding, Competitive - physiology ; Calcium - metabolism ; Calcium - pharmacology ; Calcium Signaling - physiology ; Cell Differentiation - drug effects ; Cell Differentiation - physiology ; Cell Division - drug effects ; Cell Division - physiology ; Cell Line, Transformed ; Cyclin-Dependent Kinase Inhibitor p21 ; Cyclins - metabolism ; Epidermal Cells ; Epidermis - growth & development ; Epidermis - metabolism ; Extracellular Fluid - drug effects ; Extracellular Fluid - metabolism ; Humans ; Keratinocytes - cytology ; Keratinocytes - drug effects ; Keratinocytes - metabolism ; Nucleolin ; Phosphoproteins - metabolism ; Phosphorylation - drug effects ; Protein Binding - drug effects ; Protein Binding - physiology ; RNA-Binding Proteins - metabolism ; S100 Proteins - metabolism ; Sp1 Transcription Factor - metabolism</subject><ispartof>The Journal of cell biology, 2003-11, Vol.163 (4), p.825</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14623863$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sakaguchi, Masakiyo</creatorcontrib><creatorcontrib>Miyazaki, Masahiro</creatorcontrib><creatorcontrib>Takaishi, Mikiro</creatorcontrib><creatorcontrib>Sakaguchi, Yoshihiko</creatorcontrib><creatorcontrib>Makino, Eiichi</creatorcontrib><creatorcontrib>Kataoka, Noriyuki</creatorcontrib><creatorcontrib>Yamada, Hidenori</creatorcontrib><creatorcontrib>Namba, Masayoshi</creatorcontrib><creatorcontrib>Huh, Nam-ho</creatorcontrib><title>S100C/A11 is a key mediator of Ca(2+)-induced growth inhibition of human epidermal keratinocytes</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>An increase in extracellular Ca2+ induces growth arrest and differentiation of human keratinocytes in culture. We examined possible involvement of S100C/A11 in this growth regulation. On exposure of the cells to high Ca2+, S100C/A11 was specifically phosphorylated at 10Thr and 94Ser. Phosphorylation facilitated the binding of S100C/A11 to nucleolin, resulting in nuclear translocation of S100C/A11. In nuclei, S100C/A11 liberated Sp1/3 from nucleolin. The resulting free Sp1/3 transcriptionally activated p21CIP1/WAF1, a representative negative regulator of cell growth. Introduction of anti-S100C/A11 antibody into the cells largely abolished the growth inhibition induced by Ca2+ and the induction of p21CIP1/WAF1. In the human epidermis, S100C/A11 was detected in nuclei of differentiating cells in the suprabasal layers, but not in nuclei of proliferating cells in the basal layer. These results indicate that S100C/A11 is a key mediator of the Ca(2+)-induced growth inhibition of human keratinocytes in culture, and that it may be possibly involved in the growth regulation in vivo as well.</description><subject>Active Transport, Cell Nucleus - drug effects</subject><subject>Active Transport, Cell Nucleus - physiology</subject><subject>Animals</subject><subject>Antibodies - pharmacology</subject><subject>Binding, Competitive - physiology</subject><subject>Calcium - metabolism</subject><subject>Calcium - pharmacology</subject><subject>Calcium Signaling - physiology</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Division - drug effects</subject><subject>Cell Division - physiology</subject><subject>Cell Line, Transformed</subject><subject>Cyclin-Dependent Kinase Inhibitor p21</subject><subject>Cyclins - metabolism</subject><subject>Epidermal Cells</subject><subject>Epidermis - growth & development</subject><subject>Epidermis - metabolism</subject><subject>Extracellular Fluid - drug effects</subject><subject>Extracellular Fluid - metabolism</subject><subject>Humans</subject><subject>Keratinocytes - cytology</subject><subject>Keratinocytes - drug effects</subject><subject>Keratinocytes - metabolism</subject><subject>Nucleolin</subject><subject>Phosphoproteins - metabolism</subject><subject>Phosphorylation - drug effects</subject><subject>Protein Binding - drug effects</subject><subject>Protein Binding - physiology</subject><subject>RNA-Binding Proteins - metabolism</subject><subject>S100 Proteins - metabolism</subject><subject>Sp1 Transcription Factor - metabolism</subject><issn>0021-9525</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1jztPwzAYAD2AaCmMrMgjCKX9_IjjjlVUHlIlBmAunx2buDQPOa5Q_j1FwHTL6aQj5IrBnIEWi501cw4gQAIrTsgUgLNsmfN8Qs6HYQcAspDijEyYVFxoJabk_YUBlIsVYzQMFOmnG2njqoCpi7TztMQbfnebhbY6WFfRj9h9pZqGtg4mpNC1P059aLClrg-Viw3uj42IKbSdHZMbLsipx_3gLv84I2_369fyMds8PzyVq03WM85TJr1FzrBgWnFbVE45YZWQXFVGcqmV10pLA14KdK7IjV8ieMMsOgGG51rMyPVvtz-Y48C2j6HBOG7_V8U3doZSwg</recordid><startdate>20031124</startdate><enddate>20031124</enddate><creator>Sakaguchi, Masakiyo</creator><creator>Miyazaki, Masahiro</creator><creator>Takaishi, Mikiro</creator><creator>Sakaguchi, Yoshihiko</creator><creator>Makino, Eiichi</creator><creator>Kataoka, Noriyuki</creator><creator>Yamada, Hidenori</creator><creator>Namba, Masayoshi</creator><creator>Huh, Nam-ho</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20031124</creationdate><title>S100C/A11 is a key mediator of Ca(2+)-induced growth inhibition of human epidermal keratinocytes</title><author>Sakaguchi, Masakiyo ; Miyazaki, Masahiro ; Takaishi, Mikiro ; Sakaguchi, Yoshihiko ; Makino, Eiichi ; Kataoka, Noriyuki ; Yamada, Hidenori ; Namba, Masayoshi ; Huh, Nam-ho</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p122t-4fca21a71862c7de6e3c63426db42486f8684b0f43aee75bf9a0fb1cae30b2583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Active Transport, Cell Nucleus - drug effects</topic><topic>Active Transport, Cell Nucleus - physiology</topic><topic>Animals</topic><topic>Antibodies - pharmacology</topic><topic>Binding, Competitive - physiology</topic><topic>Calcium - metabolism</topic><topic>Calcium - pharmacology</topic><topic>Calcium Signaling - physiology</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Division - drug effects</topic><topic>Cell Division - physiology</topic><topic>Cell Line, Transformed</topic><topic>Cyclin-Dependent Kinase Inhibitor p21</topic><topic>Cyclins - metabolism</topic><topic>Epidermal Cells</topic><topic>Epidermis - growth & development</topic><topic>Epidermis - metabolism</topic><topic>Extracellular Fluid - drug effects</topic><topic>Extracellular Fluid - metabolism</topic><topic>Humans</topic><topic>Keratinocytes - cytology</topic><topic>Keratinocytes - drug effects</topic><topic>Keratinocytes - metabolism</topic><topic>Nucleolin</topic><topic>Phosphoproteins - metabolism</topic><topic>Phosphorylation - drug effects</topic><topic>Protein Binding - drug effects</topic><topic>Protein Binding - physiology</topic><topic>RNA-Binding Proteins - metabolism</topic><topic>S100 Proteins - metabolism</topic><topic>Sp1 Transcription Factor - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sakaguchi, Masakiyo</creatorcontrib><creatorcontrib>Miyazaki, Masahiro</creatorcontrib><creatorcontrib>Takaishi, Mikiro</creatorcontrib><creatorcontrib>Sakaguchi, Yoshihiko</creatorcontrib><creatorcontrib>Makino, Eiichi</creatorcontrib><creatorcontrib>Kataoka, Noriyuki</creatorcontrib><creatorcontrib>Yamada, Hidenori</creatorcontrib><creatorcontrib>Namba, Masayoshi</creatorcontrib><creatorcontrib>Huh, Nam-ho</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>The Journal of cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sakaguchi, Masakiyo</au><au>Miyazaki, Masahiro</au><au>Takaishi, Mikiro</au><au>Sakaguchi, Yoshihiko</au><au>Makino, Eiichi</au><au>Kataoka, Noriyuki</au><au>Yamada, Hidenori</au><au>Namba, Masayoshi</au><au>Huh, Nam-ho</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>S100C/A11 is a key mediator of Ca(2+)-induced growth inhibition of human epidermal keratinocytes</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>2003-11-24</date><risdate>2003</risdate><volume>163</volume><issue>4</issue><spage>825</spage><pages>825-</pages><issn>0021-9525</issn><abstract>An increase in extracellular Ca2+ induces growth arrest and differentiation of human keratinocytes in culture. We examined possible involvement of S100C/A11 in this growth regulation. On exposure of the cells to high Ca2+, S100C/A11 was specifically phosphorylated at 10Thr and 94Ser. Phosphorylation facilitated the binding of S100C/A11 to nucleolin, resulting in nuclear translocation of S100C/A11. In nuclei, S100C/A11 liberated Sp1/3 from nucleolin. The resulting free Sp1/3 transcriptionally activated p21CIP1/WAF1, a representative negative regulator of cell growth. Introduction of anti-S100C/A11 antibody into the cells largely abolished the growth inhibition induced by Ca2+ and the induction of p21CIP1/WAF1. In the human epidermis, S100C/A11 was detected in nuclei of differentiating cells in the suprabasal layers, but not in nuclei of proliferating cells in the basal layer. These results indicate that S100C/A11 is a key mediator of the Ca(2+)-induced growth inhibition of human keratinocytes in culture, and that it may be possibly involved in the growth regulation in vivo as well.</abstract><cop>United States</cop><pmid>14623863</pmid><doi>10.1083/jcb.200304017</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9525 |
| ispartof | The Journal of cell biology, 2003-11, Vol.163 (4), p.825 |
| issn | 0021-9525 |
| language | eng |
| recordid | cdi_pubmed_primary_14623863 |
| source | MEDLINE; Free E-Journal (出版社公開部分のみ）; Alma/SFX Local Collection |
| subjects | Active Transport, Cell Nucleus - drug effects Active Transport, Cell Nucleus - physiology Animals Antibodies - pharmacology Binding, Competitive - physiology Calcium - metabolism Calcium - pharmacology Calcium Signaling - physiology Cell Differentiation - drug effects Cell Differentiation - physiology Cell Division - drug effects Cell Division - physiology Cell Line, Transformed Cyclin-Dependent Kinase Inhibitor p21 Cyclins - metabolism Epidermal Cells Epidermis - growth & development Epidermis - metabolism Extracellular Fluid - drug effects Extracellular Fluid - metabolism Humans Keratinocytes - cytology Keratinocytes - drug effects Keratinocytes - metabolism Nucleolin Phosphoproteins - metabolism Phosphorylation - drug effects Protein Binding - drug effects Protein Binding - physiology RNA-Binding Proteins - metabolism S100 Proteins - metabolism Sp1 Transcription Factor - metabolism |
| title | S100C/A11 is a key mediator of Ca(2+)-induced growth inhibition of human epidermal keratinocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T06%3A22%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=S100C/A11%20is%20a%20key%20mediator%20of%20Ca(2+)-induced%20growth%20inhibition%20of%20human%20epidermal%20keratinocytes&rft.jtitle=The%20Journal%20of%20cell%20biology&rft.au=Sakaguchi,%20Masakiyo&rft.date=2003-11-24&rft.volume=163&rft.issue=4&rft.spage=825&rft.pages=825-&rft.issn=0021-9525&rft_id=info:doi/10.1083/jcb.200304017&rft_dat=%3Cpubmed%3E14623863%3C/pubmed%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/14623863&rfr_iscdi=true |