Phenotypic and Functional Immaturity of Human Umbilical Cord Blood T Lymphocytes
Successful implementation of bone marrow transplantation for hematopoietic reconstitution is limited by the lack of suitably HLA-matched donors and by the occurrence of graft-versus-host disease that frequently accompanies this procedure. Recent clinical reports have implied that the use of umbilica...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1992-11, Vol.89 (21), p.10006-10010 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Harris, David T. Schumacher, Michael J. Locascio, Jeffrey Besencon, Francoise J. Olson, George B. DeLuca, Dominick Shenker, Lewis Bard, Judith Boyse, Edward A. |
| description | Successful implementation of bone marrow transplantation for hematopoietic reconstitution is limited by the lack of suitably HLA-matched donors and by the occurrence of graft-versus-host disease that frequently accompanies this procedure. Recent clinical reports have implied that the use of umbilical cord blood as a source of transplantable stem cells may solve these problems. To date, definitive experiments have not been performed to assess the immunological potential of T cells found in umbilical cord blood, which could mediate graft-versus-host disease. In the present study we have observed that umbilical cord blood contains T lymphocytes that appear to be phenotypically immature. In addition, umbilical cord blood lymphocytes appeared to be functionally immature as shown by minimal responses to stimulation with interleukin 2, phytohemagglutinin, or alloantigens. Thus, umbilical cord blood may be more suitable for allogeneic transplantation than bone marrow in that these cord blood cells may not be as capable of mediating graft-versus-host disease. |
| doi_str_mv | 10.1073/pnas.89.21.10006 |
| format | Article |
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Recent clinical reports have implied that the use of umbilical cord blood as a source of transplantable stem cells may solve these problems. To date, definitive experiments have not been performed to assess the immunological potential of T cells found in umbilical cord blood, which could mediate graft-versus-host disease. In the present study we have observed that umbilical cord blood contains T lymphocytes that appear to be phenotypically immature. In addition, umbilical cord blood lymphocytes appeared to be functionally immature as shown by minimal responses to stimulation with interleukin 2, phytohemagglutinin, or alloantigens. Thus, umbilical cord blood may be more suitable for allogeneic transplantation than bone marrow in that these cord blood cells may not be as capable of mediating graft-versus-host disease.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.89.21.10006</identifier><identifier>PMID: 1438190</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Antigens, CD - analysis ; Biological and medical sciences ; Blood ; Blotting, Southern ; Bone marrow ; Cell Line ; Cord blood ; Cultured cells ; Cytotoxicity ; Cytotoxicity, Immunologic ; DNA - genetics ; DNA - isolation & purification ; Fetal Blood - immunology ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; HLA-D Antigens - analysis ; Humans ; Immunophenotyping ; Killer Cells, Natural - immunology ; Leukocytes ; Lymphocyte Culture Test, Mixed ; Lymphocytes ; Lymphokines - biosynthesis ; Lymphokines - genetics ; Medical research ; Messenger RNA ; Natural killer cells ; Phenotypes ; Polymerase Chain Reaction ; RNA, Messenger - analysis ; T lymphocytes ; T-Lymphocytes - immunology ; Tissue, organ and graft immunology</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1992-11, Vol.89 (21), p.10006-10010</ispartof><rights>Copyright 1992 The National Academy of Sciences of the United States of America</rights><rights>1993 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Nov 1, 1992</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4696-4211fc615f0e51b5f80d21bf2a6c7043dce036e7c71537d493f088df45687f063</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/89/21.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2360537$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2360537$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,729,782,786,805,887,27931,27932,53798,53800,58024,58257</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4472441$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1438190$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harris, David T.</creatorcontrib><creatorcontrib>Schumacher, Michael J.</creatorcontrib><creatorcontrib>Locascio, Jeffrey</creatorcontrib><creatorcontrib>Besencon, Francoise J.</creatorcontrib><creatorcontrib>Olson, George B.</creatorcontrib><creatorcontrib>DeLuca, Dominick</creatorcontrib><creatorcontrib>Shenker, Lewis</creatorcontrib><creatorcontrib>Bard, Judith</creatorcontrib><creatorcontrib>Boyse, Edward A.</creatorcontrib><title>Phenotypic and Functional Immaturity of Human Umbilical Cord Blood T Lymphocytes</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Successful implementation of bone marrow transplantation for hematopoietic reconstitution is limited by the lack of suitably HLA-matched donors and by the occurrence of graft-versus-host disease that frequently accompanies this procedure. Recent clinical reports have implied that the use of umbilical cord blood as a source of transplantable stem cells may solve these problems. To date, definitive experiments have not been performed to assess the immunological potential of T cells found in umbilical cord blood, which could mediate graft-versus-host disease. In the present study we have observed that umbilical cord blood contains T lymphocytes that appear to be phenotypically immature. In addition, umbilical cord blood lymphocytes appeared to be functionally immature as shown by minimal responses to stimulation with interleukin 2, phytohemagglutinin, or alloantigens. Thus, umbilical cord blood may be more suitable for allogeneic transplantation than bone marrow in that these cord blood cells may not be as capable of mediating graft-versus-host disease.</description><subject>Animals</subject><subject>Antigens, CD - analysis</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Blotting, Southern</subject><subject>Bone marrow</subject><subject>Cell Line</subject><subject>Cord blood</subject><subject>Cultured cells</subject><subject>Cytotoxicity</subject><subject>Cytotoxicity, Immunologic</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>Fetal Blood - immunology</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>HLA-D Antigens - analysis</subject><subject>Humans</subject><subject>Immunophenotyping</subject><subject>Killer Cells, Natural - immunology</subject><subject>Leukocytes</subject><subject>Lymphocyte Culture Test, Mixed</subject><subject>Lymphocytes</subject><subject>Lymphokines - biosynthesis</subject><subject>Lymphokines - genetics</subject><subject>Medical research</subject><subject>Messenger RNA</subject><subject>Natural killer cells</subject><subject>Phenotypes</subject><subject>Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>T lymphocytes</subject><subject>T-Lymphocytes - immunology</subject><subject>Tissue, organ and graft immunology</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuP0zAURiMEGsrAngWICCHEJuX6ETuW2EDFMCNVYhYza8t1bJoqsYvtIPLvcWgpDAtYWdZ3vuvHKYqnCJYIOHm7dyouG7HEKO8B2L1igUCgilEB94sFAOZVQzF9WDyKcZcJUTdwVpwhShokYFFcX2-N82nad7pUri0vRqdT553qy6thUGkMXZpKb8vLcVCuvB02Xd_pnK58aMsPvfdteVOup2G_9XpKJj4uHljVR_PkuJ4Xtxcfb1aX1frzp6vV-3WlKROsohghqxmqLZgabWrbQIvRxmLFNAdKWm2AMMM1RzXhLRXEQtO0ltas4RYYOS_eHebux81gMu5SUL3ch25QYZJedfJu4rqt_OK_yRowm-uvj_Xgv44mJjl0UZu-V874MUpOCEWAyX9BxAgRQCGDL_8Cd34M-SOjxIAwZ1zM0-AA6eBjDMaeLoxAzkblbFQ2QmIkfxrNled_PvR34aAw56-OuYpZjA3K6S6eMEo5phRl7MURmw_4ld496M2_CWnHvk_me8roswO6i8mHE4sJg2yL_AD-mcpM</recordid><startdate>19921101</startdate><enddate>19921101</enddate><creator>Harris, David T.</creator><creator>Schumacher, Michael J.</creator><creator>Locascio, Jeffrey</creator><creator>Besencon, Francoise J.</creator><creator>Olson, George B.</creator><creator>DeLuca, Dominick</creator><creator>Shenker, Lewis</creator><creator>Bard, Judith</creator><creator>Boyse, Edward A.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19921101</creationdate><title>Phenotypic and Functional Immaturity of Human Umbilical Cord Blood T Lymphocytes</title><author>Harris, David T. ; Schumacher, Michael J. ; Locascio, Jeffrey ; Besencon, Francoise J. ; Olson, George B. ; DeLuca, Dominick ; Shenker, Lewis ; Bard, Judith ; Boyse, Edward A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4696-4211fc615f0e51b5f80d21bf2a6c7043dce036e7c71537d493f088df45687f063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Antigens, CD - analysis</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Blotting, Southern</topic><topic>Bone marrow</topic><topic>Cell Line</topic><topic>Cord blood</topic><topic>Cultured cells</topic><topic>Cytotoxicity</topic><topic>Cytotoxicity, Immunologic</topic><topic>DNA - genetics</topic><topic>DNA - isolation & purification</topic><topic>Fetal Blood - immunology</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>HLA-D Antigens - analysis</topic><topic>Humans</topic><topic>Immunophenotyping</topic><topic>Killer Cells, Natural - immunology</topic><topic>Leukocytes</topic><topic>Lymphocyte Culture Test, Mixed</topic><topic>Lymphocytes</topic><topic>Lymphokines - biosynthesis</topic><topic>Lymphokines - genetics</topic><topic>Medical research</topic><topic>Messenger RNA</topic><topic>Natural killer cells</topic><topic>Phenotypes</topic><topic>Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>T lymphocytes</topic><topic>T-Lymphocytes - immunology</topic><topic>Tissue, organ and graft immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harris, David T.</creatorcontrib><creatorcontrib>Schumacher, Michael J.</creatorcontrib><creatorcontrib>Locascio, Jeffrey</creatorcontrib><creatorcontrib>Besencon, Francoise J.</creatorcontrib><creatorcontrib>Olson, George B.</creatorcontrib><creatorcontrib>DeLuca, Dominick</creatorcontrib><creatorcontrib>Shenker, Lewis</creatorcontrib><creatorcontrib>Bard, Judith</creatorcontrib><creatorcontrib>Boyse, Edward A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harris, David T.</au><au>Schumacher, Michael J.</au><au>Locascio, Jeffrey</au><au>Besencon, Francoise J.</au><au>Olson, George B.</au><au>DeLuca, Dominick</au><au>Shenker, Lewis</au><au>Bard, Judith</au><au>Boyse, Edward A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenotypic and Functional Immaturity of Human Umbilical Cord Blood T Lymphocytes</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1992-11-01</date><risdate>1992</risdate><volume>89</volume><issue>21</issue><spage>10006</spage><epage>10010</epage><pages>10006-10010</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Successful implementation of bone marrow transplantation for hematopoietic reconstitution is limited by the lack of suitably HLA-matched donors and by the occurrence of graft-versus-host disease that frequently accompanies this procedure. Recent clinical reports have implied that the use of umbilical cord blood as a source of transplantable stem cells may solve these problems. To date, definitive experiments have not been performed to assess the immunological potential of T cells found in umbilical cord blood, which could mediate graft-versus-host disease. In the present study we have observed that umbilical cord blood contains T lymphocytes that appear to be phenotypically immature. In addition, umbilical cord blood lymphocytes appeared to be functionally immature as shown by minimal responses to stimulation with interleukin 2, phytohemagglutinin, or alloantigens. Thus, umbilical cord blood may be more suitable for allogeneic transplantation than bone marrow in that these cord blood cells may not be as capable of mediating graft-versus-host disease.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1438190</pmid><doi>10.1073/pnas.89.21.10006</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1992-11, Vol.89 (21), p.10006-10010 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Antigens, CD - analysis Biological and medical sciences Blood Blotting, Southern Bone marrow Cell Line Cord blood Cultured cells Cytotoxicity Cytotoxicity, Immunologic DNA - genetics DNA - isolation & purification Fetal Blood - immunology Flow Cytometry Fundamental and applied biological sciences. Psychology Fundamental immunology HLA-D Antigens - analysis Humans Immunophenotyping Killer Cells, Natural - immunology Leukocytes Lymphocyte Culture Test, Mixed Lymphocytes Lymphokines - biosynthesis Lymphokines - genetics Medical research Messenger RNA Natural killer cells Phenotypes Polymerase Chain Reaction RNA, Messenger - analysis T lymphocytes T-Lymphocytes - immunology Tissue, organ and graft immunology |
| title | Phenotypic and Functional Immaturity of Human Umbilical Cord Blood T Lymphocytes |
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