An improved fluorometric assay for dosimetry of benzo(a)pyrene diol-epoxide-DNA adducts in smokers' lung : comparisons with total bulky adducts and aryl hydrocarbon hydroxylase activity
An improved high-performance liquid chromatography/fluorometric assay has been established to quantitate the benzo(a)pyrene (BP) tetrols released after acid hydrolysis of lung DNA from lung cancer patients, so that the formation of benzo(a)pyrene diol-epoxide-DNA adducts can be measured. The r-7,c-1...
Gespeichert in:
| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 1992-11, Vol.52 (22), p.6248-6253 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 6253 |
|---|---|
| container_issue | 22 |
| container_start_page | 6248 |
| container_title | Cancer research (Chicago, Ill.) |
| container_volume | 52 |
| creator | ALEXANDROV, K ROJAS, M GENESTE, O CASTEGNARO, M CAMUS, A.-M PETRUZZELLI, S GIUNTINI, C BARTSCH, H |
| description | An improved high-performance liquid chromatography/fluorometric assay has been established to quantitate the benzo(a)pyrene (BP) tetrols released after acid hydrolysis of lung DNA from lung cancer patients, so that the formation of benzo(a)pyrene diol-epoxide-DNA adducts can be measured. The r-7,c-10,t-8,t-9-tetrahydroxy-7,8,9,10-tetrahydro-BP isolated by high-performance liquid chromatography was determined by chromatography in two different solvent systems and fluorescence spectroscopy. This assay has a detection limit of 2 pg of r-7,c-10,t-8,t-9-tetrahydroxy- 7,8,9,10-tetrahydro-BP, requires 100-500 micrograms of DNA, and can measure 1 adduct/10(8) unmodified nucleotides. As this assay does not use immunoaffinity chromatography or solvent extraction, it allows a > 90% recovery of benzo(a)pyrene diol-epoxide-DNA adducts. This procedure has been tested on 13 DNA samples prepared from nontumorous lung parenchyma taken from lung cancer patients at surgery and revealed the presence of DNA adducts of the anti-benzo(a)pyrene diol-epoxide in 9 of 11 samples from smokers and in 2 of 2 ex-smokers. In only two samples from smokers the formation of adducts derived from syn-benzo(a)pyrene diol-epoxide was detected. A 15-fold variation in DNA adduct level was found in 11 of 13 DNA samples, with a range of 0.6-9.9 adducts of benzo(a)pyrene diol-epoxide/10(8) nucleotides. In samples containing both anti- and syn-benzo(a)pyrene diolepoxide-DNA adducts, the anti/syn adduct ratio is 2:1. A highly significant correlation was found between pulmonary microsomal aryl hydrocarbon hydroxylase activity and the level of benzo(a)pyrene diolepoxide-DNA adduct (r = 0.91; P < 0.001; n = 13). A crude linear correlation between the amounts of these adducts and those of bulky DNA adducts determined by 32P-postlabeling assay was observed in the same samples (r = 0.78; P < 0.02; n = 13). Thus this highly sensitive and specific procedure is suitable for measuring benzo(a)pyrene diolepoxide-DNA adducts in human tissues from environmentally exposed subjects and could be adapted to measure polycyclic aromatic hydrocarbons other than BP. |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_pasca</sourceid><recordid>TN_cdi_pubmed_primary_1423269</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1423269</sourcerecordid><originalsourceid>FETCH-LOGICAL-h201t-f2b2c92e5bc895b6a980c0f0bff54ad2dffb6d57537c77b299a7d973e4fbb9153</originalsourceid><addsrcrecordid>eNo9UMtOwzAQjBColMInIO0BCThESpy4SbhV5SlVcIFztX5RU8eO7KQ0_Bl_R1CrnnZ2Zmc1mqNonNKsjIs8p8fROEmSMqZ5QU6jsxC-hpWmCR1FozQnGZlW4-h3ZkHXjXcbKUCZznlXy9ZrDhgC9qCcB-GC_id7cAqYtD_uBm-b3ksrQWhnYtm4rRYyvn-dAQrR8TaAthBqt5Y-XIPp7CfcAXd1g14HZwN863YFrWvRAOvMuj_40ApA3xtY9cI7jp45u8Pb3mCQgLzVG93259GJQhPkxX5Ooo_Hh_f5c7x4e3qZzxbxiiRpGyvCCK-IpIyXFWVTrMqEJyphStEcBRFKsamgBc0KXhSMVBUWoioymSvGqqHMSXS5-9t0rJZi2XhdDwGX-woH_WqvY-BolEfLdTic5XlKaEmyPynhgMQ</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>An improved fluorometric assay for dosimetry of benzo(a)pyrene diol-epoxide-DNA adducts in smokers' lung : comparisons with total bulky adducts and aryl hydrocarbon hydroxylase activity</title><source>MEDLINE</source><source>American Association for Cancer Research</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>ALEXANDROV, K ; ROJAS, M ; GENESTE, O ; CASTEGNARO, M ; CAMUS, A.-M ; PETRUZZELLI, S ; GIUNTINI, C ; BARTSCH, H</creator><creatorcontrib>ALEXANDROV, K ; ROJAS, M ; GENESTE, O ; CASTEGNARO, M ; CAMUS, A.-M ; PETRUZZELLI, S ; GIUNTINI, C ; BARTSCH, H</creatorcontrib><description>An improved high-performance liquid chromatography/fluorometric assay has been established to quantitate the benzo(a)pyrene (BP) tetrols released after acid hydrolysis of lung DNA from lung cancer patients, so that the formation of benzo(a)pyrene diol-epoxide-DNA adducts can be measured. The r-7,c-10,t-8,t-9-tetrahydroxy-7,8,9,10-tetrahydro-BP isolated by high-performance liquid chromatography was determined by chromatography in two different solvent systems and fluorescence spectroscopy. This assay has a detection limit of 2 pg of r-7,c-10,t-8,t-9-tetrahydroxy- 7,8,9,10-tetrahydro-BP, requires 100-500 micrograms of DNA, and can measure 1 adduct/10(8) unmodified nucleotides. As this assay does not use immunoaffinity chromatography or solvent extraction, it allows a > 90% recovery of benzo(a)pyrene diol-epoxide-DNA adducts. This procedure has been tested on 13 DNA samples prepared from nontumorous lung parenchyma taken from lung cancer patients at surgery and revealed the presence of DNA adducts of the anti-benzo(a)pyrene diol-epoxide in 9 of 11 samples from smokers and in 2 of 2 ex-smokers. In only two samples from smokers the formation of adducts derived from syn-benzo(a)pyrene diol-epoxide was detected. A 15-fold variation in DNA adduct level was found in 11 of 13 DNA samples, with a range of 0.6-9.9 adducts of benzo(a)pyrene diol-epoxide/10(8) nucleotides. In samples containing both anti- and syn-benzo(a)pyrene diolepoxide-DNA adducts, the anti/syn adduct ratio is 2:1. A highly significant correlation was found between pulmonary microsomal aryl hydrocarbon hydroxylase activity and the level of benzo(a)pyrene diolepoxide-DNA adduct (r = 0.91; P < 0.001; n = 13). A crude linear correlation between the amounts of these adducts and those of bulky DNA adducts determined by 32P-postlabeling assay was observed in the same samples (r = 0.78; P < 0.02; n = 13). Thus this highly sensitive and specific procedure is suitable for measuring benzo(a)pyrene diolepoxide-DNA adducts in human tissues from environmentally exposed subjects and could be adapted to measure polycyclic aromatic hydrocarbons other than BP.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>PMID: 1423269</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - analysis ; 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - isolation & purification ; 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - metabolism ; 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - pharmacology ; Adult ; Aged ; Animals ; Aryl Hydrocarbon Hydroxylases - metabolism ; Benzo(a)pyrene - analogs & derivatives ; Benzo(a)pyrene - analysis ; Biological and medical sciences ; Carcinogenesis, carcinogens and anticarcinogens ; Cattle ; Chemical agents ; Chromatography, High Pressure Liquid ; DNA - analysis ; DNA - isolation & purification ; DNA - metabolism ; DNA Adducts ; Fluorometry - methods ; Genetic Variation - physiology ; Humans ; Hydrolysis ; Isotope Labeling ; Lung - chemistry ; Lung - enzymology ; Lung Diseases - enzymology ; Lung Diseases - metabolism ; Lung Neoplasms - enzymology ; Lung Neoplasms - metabolism ; Male ; Medical sciences ; Microsomes - enzymology ; Middle Aged ; Phosphorus Radioisotopes ; Radiometry - methods ; Smoking - metabolism ; Thymus Gland - chemistry ; Tumors</subject><ispartof>Cancer research (Chicago, Ill.), 1992-11, Vol.52 (22), p.6248-6253</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4412582$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1423269$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ALEXANDROV, K</creatorcontrib><creatorcontrib>ROJAS, M</creatorcontrib><creatorcontrib>GENESTE, O</creatorcontrib><creatorcontrib>CASTEGNARO, M</creatorcontrib><creatorcontrib>CAMUS, A.-M</creatorcontrib><creatorcontrib>PETRUZZELLI, S</creatorcontrib><creatorcontrib>GIUNTINI, C</creatorcontrib><creatorcontrib>BARTSCH, H</creatorcontrib><title>An improved fluorometric assay for dosimetry of benzo(a)pyrene diol-epoxide-DNA adducts in smokers' lung : comparisons with total bulky adducts and aryl hydrocarbon hydroxylase activity</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>An improved high-performance liquid chromatography/fluorometric assay has been established to quantitate the benzo(a)pyrene (BP) tetrols released after acid hydrolysis of lung DNA from lung cancer patients, so that the formation of benzo(a)pyrene diol-epoxide-DNA adducts can be measured. The r-7,c-10,t-8,t-9-tetrahydroxy-7,8,9,10-tetrahydro-BP isolated by high-performance liquid chromatography was determined by chromatography in two different solvent systems and fluorescence spectroscopy. This assay has a detection limit of 2 pg of r-7,c-10,t-8,t-9-tetrahydroxy- 7,8,9,10-tetrahydro-BP, requires 100-500 micrograms of DNA, and can measure 1 adduct/10(8) unmodified nucleotides. As this assay does not use immunoaffinity chromatography or solvent extraction, it allows a > 90% recovery of benzo(a)pyrene diol-epoxide-DNA adducts. This procedure has been tested on 13 DNA samples prepared from nontumorous lung parenchyma taken from lung cancer patients at surgery and revealed the presence of DNA adducts of the anti-benzo(a)pyrene diol-epoxide in 9 of 11 samples from smokers and in 2 of 2 ex-smokers. In only two samples from smokers the formation of adducts derived from syn-benzo(a)pyrene diol-epoxide was detected. A 15-fold variation in DNA adduct level was found in 11 of 13 DNA samples, with a range of 0.6-9.9 adducts of benzo(a)pyrene diol-epoxide/10(8) nucleotides. In samples containing both anti- and syn-benzo(a)pyrene diolepoxide-DNA adducts, the anti/syn adduct ratio is 2:1. A highly significant correlation was found between pulmonary microsomal aryl hydrocarbon hydroxylase activity and the level of benzo(a)pyrene diolepoxide-DNA adduct (r = 0.91; P < 0.001; n = 13). A crude linear correlation between the amounts of these adducts and those of bulky DNA adducts determined by 32P-postlabeling assay was observed in the same samples (r = 0.78; P < 0.02; n = 13). Thus this highly sensitive and specific procedure is suitable for measuring benzo(a)pyrene diolepoxide-DNA adducts in human tissues from environmentally exposed subjects and could be adapted to measure polycyclic aromatic hydrocarbons other than BP.</description><subject>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - analysis</subject><subject>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - isolation & purification</subject><subject>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - metabolism</subject><subject>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - pharmacology</subject><subject>Adult</subject><subject>Aged</subject><subject>Animals</subject><subject>Aryl Hydrocarbon Hydroxylases - metabolism</subject><subject>Benzo(a)pyrene - analogs & derivatives</subject><subject>Benzo(a)pyrene - analysis</subject><subject>Biological and medical sciences</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>Cattle</subject><subject>Chemical agents</subject><subject>Chromatography, High Pressure Liquid</subject><subject>DNA - analysis</subject><subject>DNA - isolation & purification</subject><subject>DNA - metabolism</subject><subject>DNA Adducts</subject><subject>Fluorometry - methods</subject><subject>Genetic Variation - physiology</subject><subject>Humans</subject><subject>Hydrolysis</subject><subject>Isotope Labeling</subject><subject>Lung - chemistry</subject><subject>Lung - enzymology</subject><subject>Lung Diseases - enzymology</subject><subject>Lung Diseases - metabolism</subject><subject>Lung Neoplasms - enzymology</subject><subject>Lung Neoplasms - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microsomes - enzymology</subject><subject>Middle Aged</subject><subject>Phosphorus Radioisotopes</subject><subject>Radiometry - methods</subject><subject>Smoking - metabolism</subject><subject>Thymus Gland - chemistry</subject><subject>Tumors</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UMtOwzAQjBColMInIO0BCThESpy4SbhV5SlVcIFztX5RU8eO7KQ0_Bl_R1CrnnZ2Zmc1mqNonNKsjIs8p8fROEmSMqZ5QU6jsxC-hpWmCR1FozQnGZlW4-h3ZkHXjXcbKUCZznlXy9ZrDhgC9qCcB-GC_id7cAqYtD_uBm-b3ksrQWhnYtm4rRYyvn-dAQrR8TaAthBqt5Y-XIPp7CfcAXd1g14HZwN863YFrWvRAOvMuj_40ApA3xtY9cI7jp45u8Pb3mCQgLzVG93259GJQhPkxX5Ooo_Hh_f5c7x4e3qZzxbxiiRpGyvCCK-IpIyXFWVTrMqEJyphStEcBRFKsamgBc0KXhSMVBUWoioymSvGqqHMSXS5-9t0rJZi2XhdDwGX-woH_WqvY-BolEfLdTic5XlKaEmyPynhgMQ</recordid><startdate>19921115</startdate><enddate>19921115</enddate><creator>ALEXANDROV, K</creator><creator>ROJAS, M</creator><creator>GENESTE, O</creator><creator>CASTEGNARO, M</creator><creator>CAMUS, A.-M</creator><creator>PETRUZZELLI, S</creator><creator>GIUNTINI, C</creator><creator>BARTSCH, H</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>19921115</creationdate><title>An improved fluorometric assay for dosimetry of benzo(a)pyrene diol-epoxide-DNA adducts in smokers' lung : comparisons with total bulky adducts and aryl hydrocarbon hydroxylase activity</title><author>ALEXANDROV, K ; ROJAS, M ; GENESTE, O ; CASTEGNARO, M ; CAMUS, A.-M ; PETRUZZELLI, S ; GIUNTINI, C ; BARTSCH, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h201t-f2b2c92e5bc895b6a980c0f0bff54ad2dffb6d57537c77b299a7d973e4fbb9153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - analysis</topic><topic>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - isolation & purification</topic><topic>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - metabolism</topic><topic>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - pharmacology</topic><topic>Adult</topic><topic>Aged</topic><topic>Animals</topic><topic>Aryl Hydrocarbon Hydroxylases - metabolism</topic><topic>Benzo(a)pyrene - analogs & derivatives</topic><topic>Benzo(a)pyrene - analysis</topic><topic>Biological and medical sciences</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>Cattle</topic><topic>Chemical agents</topic><topic>Chromatography, High Pressure Liquid</topic><topic>DNA - analysis</topic><topic>DNA - isolation & purification</topic><topic>DNA - metabolism</topic><topic>DNA Adducts</topic><topic>Fluorometry - methods</topic><topic>Genetic Variation - physiology</topic><topic>Humans</topic><topic>Hydrolysis</topic><topic>Isotope Labeling</topic><topic>Lung - chemistry</topic><topic>Lung - enzymology</topic><topic>Lung Diseases - enzymology</topic><topic>Lung Diseases - metabolism</topic><topic>Lung Neoplasms - enzymology</topic><topic>Lung Neoplasms - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microsomes - enzymology</topic><topic>Middle Aged</topic><topic>Phosphorus Radioisotopes</topic><topic>Radiometry - methods</topic><topic>Smoking - metabolism</topic><topic>Thymus Gland - chemistry</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ALEXANDROV, K</creatorcontrib><creatorcontrib>ROJAS, M</creatorcontrib><creatorcontrib>GENESTE, O</creatorcontrib><creatorcontrib>CASTEGNARO, M</creatorcontrib><creatorcontrib>CAMUS, A.-M</creatorcontrib><creatorcontrib>PETRUZZELLI, S</creatorcontrib><creatorcontrib>GIUNTINI, C</creatorcontrib><creatorcontrib>BARTSCH, H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Cancer research (Chicago, Ill.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ALEXANDROV, K</au><au>ROJAS, M</au><au>GENESTE, O</au><au>CASTEGNARO, M</au><au>CAMUS, A.-M</au><au>PETRUZZELLI, S</au><au>GIUNTINI, C</au><au>BARTSCH, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An improved fluorometric assay for dosimetry of benzo(a)pyrene diol-epoxide-DNA adducts in smokers' lung : comparisons with total bulky adducts and aryl hydrocarbon hydroxylase activity</atitle><jtitle>Cancer research (Chicago, Ill.)</jtitle><addtitle>Cancer Res</addtitle><date>1992-11-15</date><risdate>1992</risdate><volume>52</volume><issue>22</issue><spage>6248</spage><epage>6253</epage><pages>6248-6253</pages><issn>0008-5472</issn><eissn>1538-7445</eissn><coden>CNREA8</coden><abstract>An improved high-performance liquid chromatography/fluorometric assay has been established to quantitate the benzo(a)pyrene (BP) tetrols released after acid hydrolysis of lung DNA from lung cancer patients, so that the formation of benzo(a)pyrene diol-epoxide-DNA adducts can be measured. The r-7,c-10,t-8,t-9-tetrahydroxy-7,8,9,10-tetrahydro-BP isolated by high-performance liquid chromatography was determined by chromatography in two different solvent systems and fluorescence spectroscopy. This assay has a detection limit of 2 pg of r-7,c-10,t-8,t-9-tetrahydroxy- 7,8,9,10-tetrahydro-BP, requires 100-500 micrograms of DNA, and can measure 1 adduct/10(8) unmodified nucleotides. As this assay does not use immunoaffinity chromatography or solvent extraction, it allows a > 90% recovery of benzo(a)pyrene diol-epoxide-DNA adducts. This procedure has been tested on 13 DNA samples prepared from nontumorous lung parenchyma taken from lung cancer patients at surgery and revealed the presence of DNA adducts of the anti-benzo(a)pyrene diol-epoxide in 9 of 11 samples from smokers and in 2 of 2 ex-smokers. In only two samples from smokers the formation of adducts derived from syn-benzo(a)pyrene diol-epoxide was detected. A 15-fold variation in DNA adduct level was found in 11 of 13 DNA samples, with a range of 0.6-9.9 adducts of benzo(a)pyrene diol-epoxide/10(8) nucleotides. In samples containing both anti- and syn-benzo(a)pyrene diolepoxide-DNA adducts, the anti/syn adduct ratio is 2:1. A highly significant correlation was found between pulmonary microsomal aryl hydrocarbon hydroxylase activity and the level of benzo(a)pyrene diolepoxide-DNA adduct (r = 0.91; P < 0.001; n = 13). A crude linear correlation between the amounts of these adducts and those of bulky DNA adducts determined by 32P-postlabeling assay was observed in the same samples (r = 0.78; P < 0.02; n = 13). Thus this highly sensitive and specific procedure is suitable for measuring benzo(a)pyrene diolepoxide-DNA adducts in human tissues from environmentally exposed subjects and could be adapted to measure polycyclic aromatic hydrocarbons other than BP.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>1423269</pmid><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0008-5472 |
| ispartof | Cancer research (Chicago, Ill.), 1992-11, Vol.52 (22), p.6248-6253 |
| issn | 0008-5472 1538-7445 |
| language | eng |
| recordid | cdi_pubmed_primary_1423269 |
| source | MEDLINE; American Association for Cancer Research; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - analysis 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - isolation & purification 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - metabolism 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - pharmacology Adult Aged Animals Aryl Hydrocarbon Hydroxylases - metabolism Benzo(a)pyrene - analogs & derivatives Benzo(a)pyrene - analysis Biological and medical sciences Carcinogenesis, carcinogens and anticarcinogens Cattle Chemical agents Chromatography, High Pressure Liquid DNA - analysis DNA - isolation & purification DNA - metabolism DNA Adducts Fluorometry - methods Genetic Variation - physiology Humans Hydrolysis Isotope Labeling Lung - chemistry Lung - enzymology Lung Diseases - enzymology Lung Diseases - metabolism Lung Neoplasms - enzymology Lung Neoplasms - metabolism Male Medical sciences Microsomes - enzymology Middle Aged Phosphorus Radioisotopes Radiometry - methods Smoking - metabolism Thymus Gland - chemistry Tumors |
| title | An improved fluorometric assay for dosimetry of benzo(a)pyrene diol-epoxide-DNA adducts in smokers' lung : comparisons with total bulky adducts and aryl hydrocarbon hydroxylase activity |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T05%3A48%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=An%20improved%20fluorometric%20assay%20for%20dosimetry%20of%20benzo(a)pyrene%20diol-epoxide-DNA%20adducts%20in%20smokers'%20lung%20:%20comparisons%20with%20total%20bulky%20adducts%20and%20aryl%20hydrocarbon%20hydroxylase%20activity&rft.jtitle=Cancer%20research%20(Chicago,%20Ill.)&rft.au=ALEXANDROV,%20K&rft.date=1992-11-15&rft.volume=52&rft.issue=22&rft.spage=6248&rft.epage=6253&rft.pages=6248-6253&rft.issn=0008-5472&rft.eissn=1538-7445&rft.coden=CNREA8&rft_id=info:doi/&rft_dat=%3Cpubmed_pasca%3E1423269%3C/pubmed_pasca%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/1423269&rfr_iscdi=true |