CHARACTERIZATION AND NUCLEOTIDE-SEQUENCES OF THE VARIABLE REGIONS OF A MONOCLONAL-ANTIBODY AGAINST ALPHA-FETOPROTEIN
Alpha-Fetoprotein (AFP) is a well-known tumor marker of hepatocellular carcinoma (HCC). Monoclonal antibodies against AFP possessing specific binding ability to HCC are potential candidates for immunoscintigraphy and immunotherapy. A new monoclonal antibody against AFP (0325-6-9) was isolated. Its s...
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| Veröffentlicht in: | Hybridoma 1992-06, Vol.11 (3), p.339-350 |
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| creator | HONG, TH HSIAO, SC SHEU, WJ CHANG, TH |
| description | Alpha-Fetoprotein (AFP) is a well-known tumor marker of hepatocellular carcinoma (HCC). Monoclonal antibodies against AFP possessing specific binding ability to HCC are potential candidates for immunoscintigraphy and immunotherapy. A new monoclonal antibody against AFP (0325-6-9) was isolated. Its specificity and targeting tumor ability were characterized by enzyme-linked immunosorbent assay (ELISA), cell immunostain and complement killing. These results suggest that 0325-6-9 is specific to hepatoma cells. The nucleotide sequences of variable regions of 0325-6-9 were determined by M13 dideoxynucleotide sequencing method. With the information of nucleotide sequence, this antibody then could be modified by recombinant technology for its usage in in vivo diagnosis and immunotherapy. |
| doi_str_mv | 10.1089/hyb.1992.11.339 |
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Monoclonal antibodies against AFP possessing specific binding ability to HCC are potential candidates for immunoscintigraphy and immunotherapy. A new monoclonal antibody against AFP (0325-6-9) was isolated. Its specificity and targeting tumor ability were characterized by enzyme-linked immunosorbent assay (ELISA), cell immunostain and complement killing. These results suggest that 0325-6-9 is specific to hepatoma cells. The nucleotide sequences of variable regions of 0325-6-9 were determined by M13 dideoxynucleotide sequencing method. With the information of nucleotide sequence, this antibody then could be modified by recombinant technology for its usage in in vivo diagnosis and immunotherapy.</description><identifier>ISSN: 0272-457X</identifier><identifier>EISSN: 2168-7897</identifier><identifier>EISSN: 0272-457X</identifier><identifier>DOI: 10.1089/hyb.1992.11.339</identifier><identifier>PMID: 1379974</identifier><identifier>CODEN: HYBRDY</identifier><language>eng</language><publisher>LARCHMONT: MARY ANN LIEBERT INC PUBL</publisher><subject>alpha-Fetoproteins - immunology ; Amino Acid Sequence ; Antibodies, Monoclonal - genetics ; Antibody Specificity ; Base Sequence ; Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biological and medical sciences ; Biomarkers, Tumor ; Biotechnology & Applied Microbiology ; Carcinoma, Hepatocellular - diagnosis ; DNA - genetics ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; Humans ; Immunoglobulin Variable Region - genetics ; Immunology ; Life Sciences & Biomedicine ; Liver Neoplasms - diagnosis ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Science & Technology ; Tumor Cells, Cultured - immunology</subject><ispartof>Hybridoma, 1992-06, Vol.11 (3), p.339-350</ispartof><rights>1993 INIST-CNRS</rights><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>1</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wosA1992JK41900008</woscitedreferencesoriginalsourcerecordid><cites>FETCH-LOGICAL-c275t-55e8bf9f14e3fe6ef46587a2e18bcb4a5d03f3a0e0f2b11d437e66884e1536163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,3043,27197,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4462739$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1379974$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HONG, TH</creatorcontrib><creatorcontrib>HSIAO, SC</creatorcontrib><creatorcontrib>SHEU, WJ</creatorcontrib><creatorcontrib>CHANG, TH</creatorcontrib><title>CHARACTERIZATION AND NUCLEOTIDE-SEQUENCES OF THE VARIABLE REGIONS OF A MONOCLONAL-ANTIBODY AGAINST ALPHA-FETOPROTEIN</title><title>Hybridoma</title><addtitle>HYBRIDOMA</addtitle><addtitle>Hybridoma</addtitle><description>Alpha-Fetoprotein (AFP) is a well-known tumor marker of hepatocellular carcinoma (HCC). Monoclonal antibodies against AFP possessing specific binding ability to HCC are potential candidates for immunoscintigraphy and immunotherapy. A new monoclonal antibody against AFP (0325-6-9) was isolated. Its specificity and targeting tumor ability were characterized by enzyme-linked immunosorbent assay (ELISA), cell immunostain and complement killing. These results suggest that 0325-6-9 is specific to hepatoma cells. The nucleotide sequences of variable regions of 0325-6-9 were determined by M13 dideoxynucleotide sequencing method. With the information of nucleotide sequence, this antibody then could be modified by recombinant technology for its usage in in vivo diagnosis and immunotherapy.</description><subject>alpha-Fetoproteins - immunology</subject><subject>Amino Acid Sequence</subject><subject>Antibodies, Monoclonal - genetics</subject><subject>Antibody Specificity</subject><subject>Base Sequence</subject><subject>Biochemical Research Methods</subject><subject>Biochemistry & Molecular Biology</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor</subject><subject>Biotechnology & Applied Microbiology</subject><subject>Carcinoma, Hepatocellular - diagnosis</subject><subject>DNA - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>Humans</subject><subject>Immunoglobulin Variable Region - genetics</subject><subject>Immunology</subject><subject>Life Sciences & Biomedicine</subject><subject>Liver Neoplasms - diagnosis</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Science & Technology</subject><subject>Tumor Cells, Cultured - immunology</subject><issn>0272-457X</issn><issn>2168-7897</issn><issn>0272-457X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EZCTM</sourceid><sourceid>EIF</sourceid><recordid>eNqNkc9v0zAYhiMEGmVw5oSUA-KC0vlXYvvopV4bCPFIUwRcIie1RaY2GXEqtP8ed63GldMn-X38ftLzBcFbCOYQMH7166GZQ87RHMI5xvxZMEMwYRFlnD4PZgBRFJGYfn8ZvHLuDgAQI0IvgguIKeeUzIIpXYlSpJUss5-iylQRimIRFps0l6rKFjJay68bWaRyHaqbsFrJ8JsoM3Gdy7CUS88_vovwiypUmqtC5JEoquxaLX6EYimyYl2FIr9diehGVuq2VJXMitfBC6t3zrw5z8tg49N0FeVqmaW-okU0nqI4Nqyx3EJisDWJsSSJGdXIQNa0DdHxFmCLNTDAogbCLcHUJAljxMAYJzDBl8GHU-_9OPw-GDfV-861ZrfTvRkOrqYYopgn0INXJ7AdB-dGY-v7sdvr8aGGoD56rr3n-ui5hrD2nv2Pd-fqQ7M323_8SazP359z7Vq9s6Pu2849YYQkiD7WfDxhf0wzWNd2pm_NEyWOGz99JpD7ywHmafb_dNpNeuqGPh0O_YT_Aj6UnWU</recordid><startdate>19920601</startdate><enddate>19920601</enddate><creator>HONG, TH</creator><creator>HSIAO, SC</creator><creator>SHEU, WJ</creator><creator>CHANG, TH</creator><general>MARY ANN LIEBERT INC PUBL</general><general>Liebert</general><scope>BLEPL</scope><scope>DTL</scope><scope>EZCTM</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920601</creationdate><title>CHARACTERIZATION AND NUCLEOTIDE-SEQUENCES OF THE VARIABLE REGIONS OF A MONOCLONAL-ANTIBODY AGAINST ALPHA-FETOPROTEIN</title><author>HONG, TH ; HSIAO, SC ; SHEU, WJ ; CHANG, TH</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c275t-55e8bf9f14e3fe6ef46587a2e18bcb4a5d03f3a0e0f2b11d437e66884e1536163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>alpha-Fetoproteins - immunology</topic><topic>Amino Acid Sequence</topic><topic>Antibodies, Monoclonal - genetics</topic><topic>Antibody Specificity</topic><topic>Base Sequence</topic><topic>Biochemical Research Methods</topic><topic>Biochemistry & Molecular Biology</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor</topic><topic>Biotechnology & Applied Microbiology</topic><topic>Carcinoma, Hepatocellular - diagnosis</topic><topic>DNA - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Humans</topic><topic>Immunoglobulin Variable Region - genetics</topic><topic>Immunology</topic><topic>Life Sciences & Biomedicine</topic><topic>Liver Neoplasms - diagnosis</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Science & Technology</topic><topic>Tumor Cells, Cultured - immunology</topic><toplevel>online_resources</toplevel><creatorcontrib>HONG, TH</creatorcontrib><creatorcontrib>HSIAO, SC</creatorcontrib><creatorcontrib>SHEU, WJ</creatorcontrib><creatorcontrib>CHANG, TH</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 1992</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Hybridoma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HONG, TH</au><au>HSIAO, SC</au><au>SHEU, WJ</au><au>CHANG, TH</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CHARACTERIZATION AND NUCLEOTIDE-SEQUENCES OF THE VARIABLE REGIONS OF A MONOCLONAL-ANTIBODY AGAINST ALPHA-FETOPROTEIN</atitle><jtitle>Hybridoma</jtitle><stitle>HYBRIDOMA</stitle><addtitle>Hybridoma</addtitle><date>1992-06-01</date><risdate>1992</risdate><volume>11</volume><issue>3</issue><spage>339</spage><epage>350</epage><pages>339-350</pages><issn>0272-457X</issn><eissn>2168-7897</eissn><eissn>0272-457X</eissn><coden>HYBRDY</coden><abstract>Alpha-Fetoprotein (AFP) is a well-known tumor marker of hepatocellular carcinoma (HCC). Monoclonal antibodies against AFP possessing specific binding ability to HCC are potential candidates for immunoscintigraphy and immunotherapy. A new monoclonal antibody against AFP (0325-6-9) was isolated. Its specificity and targeting tumor ability were characterized by enzyme-linked immunosorbent assay (ELISA), cell immunostain and complement killing. These results suggest that 0325-6-9 is specific to hepatoma cells. The nucleotide sequences of variable regions of 0325-6-9 were determined by M13 dideoxynucleotide sequencing method. With the information of nucleotide sequence, this antibody then could be modified by recombinant technology for its usage in in vivo diagnosis and immunotherapy.</abstract><cop>LARCHMONT</cop><pub>MARY ANN LIEBERT INC PUBL</pub><pmid>1379974</pmid><doi>10.1089/hyb.1992.11.339</doi><tpages>12</tpages></addata></record> |
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| source | Web of Science - Science Citation Index Expanded - 1992<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | alpha-Fetoproteins - immunology Amino Acid Sequence Antibodies, Monoclonal - genetics Antibody Specificity Base Sequence Biochemical Research Methods Biochemistry & Molecular Biology Biological and medical sciences Biomarkers, Tumor Biotechnology & Applied Microbiology Carcinoma, Hepatocellular - diagnosis DNA - genetics Fundamental and applied biological sciences. Psychology Genes. Genome Humans Immunoglobulin Variable Region - genetics Immunology Life Sciences & Biomedicine Liver Neoplasms - diagnosis Molecular and cellular biology Molecular genetics Molecular Sequence Data Science & Technology Tumor Cells, Cultured - immunology |
| title | CHARACTERIZATION AND NUCLEOTIDE-SEQUENCES OF THE VARIABLE REGIONS OF A MONOCLONAL-ANTIBODY AGAINST ALPHA-FETOPROTEIN |
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