SK HEP-1: A Human Cell Line of Endothelial Origin
SK-HEP-1 is an immortal, human cell line derived from the ascitic fluid of a patient with adenocarcinoma of the liver. We have determined that these cells are of endothelial origin. Despite the location of the tumor from which SK HEP-1 was derived, the cell line does not have properties of hepatocyt...
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| Veröffentlicht in: | In Vitro Cellular & Developmental Biology - Animal 1992-02, Vol.28A (2), p.136-142 |
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| container_title | In Vitro Cellular & Developmental Biology - Animal |
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| creator | Sue C. Heffelfinger Hal H. Hawkins Jim Barrish Taylor, Linda Darlington, Gretchen J. |
| description | SK-HEP-1 is an immortal, human cell line derived from the ascitic fluid of a patient with adenocarcinoma of the liver. We have determined that these cells are of endothelial origin. Despite the location of the tumor from which SK HEP-1 was derived, the cell line does not have properties of hepatocytes. Northern blot analysis of total cellular RNA shows no messenger RNA for the hepatic-specific proteins albumin, alpha-fibrinogen, or gamma-fibrinogen. Endothelial characteristics are seen by transmission electron microscopy. These features include numerous pinocytotic vesicles, electron dense granules consistent with Weibel-Palade bodies, and abundant intermediate filaments, identified immunocytochemically as vimentin. Cultures grown on plastic dishes grow in bundles of polygonal to spindle-shaped cells. Proteins characteristic for endothelial cells are identified by immunocytochemistry. Addition of basement membrane material (Matrigel) or type I collagen to the cultures induces these cells to organize into a tubular network. |
| doi_str_mv | 10.1007/bf02631017 |
| format | Article |
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Heffelfinger ; Hal H. Hawkins ; Jim Barrish ; Taylor, Linda ; Darlington, Gretchen J.</creator><creatorcontrib>Sue C. Heffelfinger ; Hal H. Hawkins ; Jim Barrish ; Taylor, Linda ; Darlington, Gretchen J.</creatorcontrib><description>SK-HEP-1 is an immortal, human cell line derived from the ascitic fluid of a patient with adenocarcinoma of the liver. We have determined that these cells are of endothelial origin. Despite the location of the tumor from which SK HEP-1 was derived, the cell line does not have properties of hepatocytes. Northern blot analysis of total cellular RNA shows no messenger RNA for the hepatic-specific proteins albumin, alpha-fibrinogen, or gamma-fibrinogen. Endothelial characteristics are seen by transmission electron microscopy. These features include numerous pinocytotic vesicles, electron dense granules consistent with Weibel-Palade bodies, and abundant intermediate filaments, identified immunocytochemically as vimentin. Cultures grown on plastic dishes grow in bundles of polygonal to spindle-shaped cells. Proteins characteristic for endothelial cells are identified by immunocytochemistry. Addition of basement membrane material (Matrigel) or type I collagen to the cultures induces these cells to organize into a tubular network.</description><identifier>ISSN: 0883-8364</identifier><identifier>ISSN: 1071-2690</identifier><identifier>EISSN: 2327-431X</identifier><identifier>EISSN: 1543-706X</identifier><identifier>DOI: 10.1007/bf02631017</identifier><identifier>PMID: 1371504</identifier><identifier>CODEN: ICDBEO</identifier><language>eng</language><publisher>COLUMBIA: Tissue Culture Association, Inc</publisher><subject>Adenocarcinoma - pathology ; Adenocarcinoma - physiopathology ; alpha 1-Antitrypsin - genetics ; Animal cells ; Biological and medical sciences ; Biotechnology ; Cell Adhesion Molecules - metabolism ; Cell Biology ; Cell culture techniques ; Cell Line ; Cell lines ; Complement C3 - genetics ; Cultured cells ; Developmental Biology ; E-Selectin ; Endothelial cells ; Endothelium - pathology ; Endothelium - physiopathology ; Establishment of new cell lines, improvement of cultural methods, mass cultures ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; Gels ; Gene Expression ; Human umbilical vein endothelial cells ; Humans ; Immunohistochemistry ; Karyotyping ; Life Sciences & Biomedicine ; Liver Neoplasms - pathology ; Liver Neoplasms - physiopathology ; Membrane Glycoproteins - metabolism ; Methods. Procedures. Technologies ; Microscopy, Electron ; Monoclonal antibodies ; Mucin-1 ; RNA ; Science & Technology ; Tumor cell line ; Tumors ; Vimentin - metabolism ; von Willebrand Factor - metabolism</subject><ispartof>In Vitro Cellular & Developmental Biology - Animal, 1992-02, Vol.28A (2), p.136-142</ispartof><rights>Copyright 1992 Tissue Culture Association</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>137</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wosA1992HH81900012</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c398t-5cfe74aa98df473601a4f3717f9b2b6b160cb9a211c61a2026e2a2f6605d4ad83</citedby><cites>FETCH-LOGICAL-c398t-5cfe74aa98df473601a4f3717f9b2b6b160cb9a211c61a2026e2a2f6605d4ad83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4296812$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4296812$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>315,782,786,805,27199,27931,27932,58024,58257</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5310421$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1371504$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sue C. Heffelfinger</creatorcontrib><creatorcontrib>Hal H. Hawkins</creatorcontrib><creatorcontrib>Jim Barrish</creatorcontrib><creatorcontrib>Taylor, Linda</creatorcontrib><creatorcontrib>Darlington, Gretchen J.</creatorcontrib><title>SK HEP-1: A Human Cell Line of Endothelial Origin</title><title>In Vitro Cellular & Developmental Biology - Animal</title><addtitle>IN VITRO CELL DEV-AN</addtitle><addtitle>In Vitro Cell Dev Biol</addtitle><description>SK-HEP-1 is an immortal, human cell line derived from the ascitic fluid of a patient with adenocarcinoma of the liver. We have determined that these cells are of endothelial origin. Despite the location of the tumor from which SK HEP-1 was derived, the cell line does not have properties of hepatocytes. Northern blot analysis of total cellular RNA shows no messenger RNA for the hepatic-specific proteins albumin, alpha-fibrinogen, or gamma-fibrinogen. Endothelial characteristics are seen by transmission electron microscopy. These features include numerous pinocytotic vesicles, electron dense granules consistent with Weibel-Palade bodies, and abundant intermediate filaments, identified immunocytochemically as vimentin. Cultures grown on plastic dishes grow in bundles of polygonal to spindle-shaped cells. Proteins characteristic for endothelial cells are identified by immunocytochemistry. Addition of basement membrane material (Matrigel) or type I collagen to the cultures induces these cells to organize into a tubular network.</description><subject>Adenocarcinoma - pathology</subject><subject>Adenocarcinoma - physiopathology</subject><subject>alpha 1-Antitrypsin - genetics</subject><subject>Animal cells</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>Cell Biology</subject><subject>Cell culture techniques</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Complement C3 - genetics</subject><subject>Cultured cells</subject><subject>Developmental Biology</subject><subject>E-Selectin</subject><subject>Endothelial cells</subject><subject>Endothelium - pathology</subject><subject>Endothelium - physiopathology</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass cultures</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Gene Expression</subject><subject>Human umbilical vein endothelial cells</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Karyotyping</subject><subject>Life Sciences & Biomedicine</subject><subject>Liver Neoplasms - pathology</subject><subject>Liver Neoplasms - physiopathology</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Methods. Procedures. Technologies</subject><subject>Microscopy, Electron</subject><subject>Monoclonal antibodies</subject><subject>Mucin-1</subject><subject>RNA</subject><subject>Science & Technology</subject><subject>Tumor cell line</subject><subject>Tumors</subject><subject>Vimentin - metabolism</subject><subject>von Willebrand Factor - metabolism</subject><issn>0883-8364</issn><issn>1071-2690</issn><issn>2327-431X</issn><issn>1543-706X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EZCTM</sourceid><sourceid>EIF</sourceid><recordid>eNqN0LFv1DAUBnALgdqjsDCDlKFioAr1e3Ych-0aXZuKk1oJKnWLHMcurnJ2iRNV_PcY5ThWJg_v9322HyHvgH4GSsvzzlIUDCiUL8gKGZY5Z3D_kqyolCyXTPBj8jrGR0oZFYhH5AhYCQXlKwLfvmbN5jaHL9k6a-ad8llthiHbOm-yYLON78P0wwxODdnN6B6cf0NeWTVE83Z_npC7y833usm3N1fX9Xqba1bJKS-0NSVXqpK95SUTFBS36dbSVh12ogNBdVcpBNACFKYPGFRohaBFz1Uv2Qn5uPQ-jeHnbOLU7lzU6W3KmzDHtkSJiKxI8NMC9RhiHI1tn0a3U-OvFmj7Zz_txeXf_ST8Yd86dzvT_6PLQtL8dD9XUavBjsprFw-sSC0cIbGzhT2bLtionfHaHNQaqgqbRkJFKQVMWv6_rt2kJhd8HWY_pej7JfoYpzAeMhwrIVPzb5hXktU</recordid><startdate>19920201</startdate><enddate>19920201</enddate><creator>Sue C. Heffelfinger</creator><creator>Hal H. Hawkins</creator><creator>Jim Barrish</creator><creator>Taylor, Linda</creator><creator>Darlington, Gretchen J.</creator><general>Tissue Culture Association, Inc</general><general>SOC IN VITRO BIOLOGY</general><general>Society for In Vitro Biology</general><scope>BLEPL</scope><scope>DTL</scope><scope>EZCTM</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920201</creationdate><title>SK HEP-1: A Human Cell Line of Endothelial Origin</title><author>Sue C. Heffelfinger ; Hal H. Hawkins ; Jim Barrish ; Taylor, Linda ; Darlington, Gretchen J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-5cfe74aa98df473601a4f3717f9b2b6b160cb9a211c61a2026e2a2f6605d4ad83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adenocarcinoma - pathology</topic><topic>Adenocarcinoma - physiopathology</topic><topic>alpha 1-Antitrypsin - genetics</topic><topic>Animal cells</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell Adhesion Molecules - metabolism</topic><topic>Cell Biology</topic><topic>Cell culture techniques</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Complement C3 - genetics</topic><topic>Cultured cells</topic><topic>Developmental Biology</topic><topic>E-Selectin</topic><topic>Endothelial cells</topic><topic>Endothelium - pathology</topic><topic>Endothelium - physiopathology</topic><topic>Establishment of new cell lines, improvement of cultural methods, mass cultures</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Gene Expression</topic><topic>Human umbilical vein endothelial cells</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Karyotyping</topic><topic>Life Sciences & Biomedicine</topic><topic>Liver Neoplasms - pathology</topic><topic>Liver Neoplasms - physiopathology</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Methods. Procedures. Technologies</topic><topic>Microscopy, Electron</topic><topic>Monoclonal antibodies</topic><topic>Mucin-1</topic><topic>RNA</topic><topic>Science & Technology</topic><topic>Tumor cell line</topic><topic>Tumors</topic><topic>Vimentin - metabolism</topic><topic>von Willebrand Factor - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sue C. Heffelfinger</creatorcontrib><creatorcontrib>Hal H. Hawkins</creatorcontrib><creatorcontrib>Jim Barrish</creatorcontrib><creatorcontrib>Taylor, Linda</creatorcontrib><creatorcontrib>Darlington, Gretchen J.</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 1992</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>In Vitro Cellular & Developmental Biology - Animal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sue C. Heffelfinger</au><au>Hal H. Hawkins</au><au>Jim Barrish</au><au>Taylor, Linda</au><au>Darlington, Gretchen J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SK HEP-1: A Human Cell Line of Endothelial Origin</atitle><jtitle>In Vitro Cellular & Developmental Biology - Animal</jtitle><stitle>IN VITRO CELL DEV-AN</stitle><addtitle>In Vitro Cell Dev Biol</addtitle><date>1992-02-01</date><risdate>1992</risdate><volume>28A</volume><issue>2</issue><spage>136</spage><epage>142</epage><pages>136-142</pages><issn>0883-8364</issn><issn>1071-2690</issn><eissn>2327-431X</eissn><eissn>1543-706X</eissn><coden>ICDBEO</coden><abstract>SK-HEP-1 is an immortal, human cell line derived from the ascitic fluid of a patient with adenocarcinoma of the liver. We have determined that these cells are of endothelial origin. Despite the location of the tumor from which SK HEP-1 was derived, the cell line does not have properties of hepatocytes. Northern blot analysis of total cellular RNA shows no messenger RNA for the hepatic-specific proteins albumin, alpha-fibrinogen, or gamma-fibrinogen. Endothelial characteristics are seen by transmission electron microscopy. These features include numerous pinocytotic vesicles, electron dense granules consistent with Weibel-Palade bodies, and abundant intermediate filaments, identified immunocytochemically as vimentin. Cultures grown on plastic dishes grow in bundles of polygonal to spindle-shaped cells. Proteins characteristic for endothelial cells are identified by immunocytochemistry. Addition of basement membrane material (Matrigel) or type I collagen to the cultures induces these cells to organize into a tubular network.</abstract><cop>COLUMBIA</cop><pub>Tissue Culture Association, Inc</pub><pmid>1371504</pmid><doi>10.1007/bf02631017</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0883-8364 |
| ispartof | In Vitro Cellular & Developmental Biology - Animal, 1992-02, Vol.28A (2), p.136-142 |
| issn | 0883-8364 1071-2690 2327-431X 1543-706X |
| language | eng |
| recordid | cdi_pubmed_primary_1371504 |
| source | Web of Science - Science Citation Index Expanded - 1992<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; MEDLINE; SpringerNature Journals; JSTOR Archive Collection A-Z Listing |
| subjects | Adenocarcinoma - pathology Adenocarcinoma - physiopathology alpha 1-Antitrypsin - genetics Animal cells Biological and medical sciences Biotechnology Cell Adhesion Molecules - metabolism Cell Biology Cell culture techniques Cell Line Cell lines Complement C3 - genetics Cultured cells Developmental Biology E-Selectin Endothelial cells Endothelium - pathology Endothelium - physiopathology Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Gels Gene Expression Human umbilical vein endothelial cells Humans Immunohistochemistry Karyotyping Life Sciences & Biomedicine Liver Neoplasms - pathology Liver Neoplasms - physiopathology Membrane Glycoproteins - metabolism Methods. Procedures. Technologies Microscopy, Electron Monoclonal antibodies Mucin-1 RNA Science & Technology Tumor cell line Tumors Vimentin - metabolism von Willebrand Factor - metabolism |
| title | SK HEP-1: A Human Cell Line of Endothelial Origin |
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