SK HEP-1: A Human Cell Line of Endothelial Origin

SK-HEP-1 is an immortal, human cell line derived from the ascitic fluid of a patient with adenocarcinoma of the liver. We have determined that these cells are of endothelial origin. Despite the location of the tumor from which SK HEP-1 was derived, the cell line does not have properties of hepatocyt...

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Veröffentlicht in:In Vitro Cellular & Developmental Biology - Animal 1992-02, Vol.28A (2), p.136-142
Hauptverfasser: Sue C. Heffelfinger, Hal H. Hawkins, Jim Barrish, Taylor, Linda, Darlington, Gretchen J.
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container_issue 2
container_start_page 136
container_title In Vitro Cellular & Developmental Biology - Animal
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creator Sue C. Heffelfinger
Hal H. Hawkins
Jim Barrish
Taylor, Linda
Darlington, Gretchen J.
description SK-HEP-1 is an immortal, human cell line derived from the ascitic fluid of a patient with adenocarcinoma of the liver. We have determined that these cells are of endothelial origin. Despite the location of the tumor from which SK HEP-1 was derived, the cell line does not have properties of hepatocytes. Northern blot analysis of total cellular RNA shows no messenger RNA for the hepatic-specific proteins albumin, alpha-fibrinogen, or gamma-fibrinogen. Endothelial characteristics are seen by transmission electron microscopy. These features include numerous pinocytotic vesicles, electron dense granules consistent with Weibel-Palade bodies, and abundant intermediate filaments, identified immunocytochemically as vimentin. Cultures grown on plastic dishes grow in bundles of polygonal to spindle-shaped cells. Proteins characteristic for endothelial cells are identified by immunocytochemistry. Addition of basement membrane material (Matrigel) or type I collagen to the cultures induces these cells to organize into a tubular network.
doi_str_mv 10.1007/bf02631017
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Heffelfinger</creatorcontrib><creatorcontrib>Hal H. Hawkins</creatorcontrib><creatorcontrib>Jim Barrish</creatorcontrib><creatorcontrib>Taylor, Linda</creatorcontrib><creatorcontrib>Darlington, Gretchen J.</creatorcontrib><title>SK HEP-1: A Human Cell Line of Endothelial Origin</title><title>In Vitro Cellular &amp; Developmental Biology - Animal</title><addtitle>IN VITRO CELL DEV-AN</addtitle><addtitle>In Vitro Cell Dev Biol</addtitle><description>SK-HEP-1 is an immortal, human cell line derived from the ascitic fluid of a patient with adenocarcinoma of the liver. We have determined that these cells are of endothelial origin. Despite the location of the tumor from which SK HEP-1 was derived, the cell line does not have properties of hepatocytes. Northern blot analysis of total cellular RNA shows no messenger RNA for the hepatic-specific proteins albumin, alpha-fibrinogen, or gamma-fibrinogen. Endothelial characteristics are seen by transmission electron microscopy. 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Psychology</subject><subject>Gels</subject><subject>Gene Expression</subject><subject>Human umbilical vein endothelial cells</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Karyotyping</subject><subject>Life Sciences &amp; Biomedicine</subject><subject>Liver Neoplasms - pathology</subject><subject>Liver Neoplasms - physiopathology</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Methods. Procedures. Technologies</subject><subject>Microscopy, Electron</subject><subject>Monoclonal antibodies</subject><subject>Mucin-1</subject><subject>RNA</subject><subject>Science &amp; Technology</subject><subject>Tumor cell line</subject><subject>Tumors</subject><subject>Vimentin - metabolism</subject><subject>von Willebrand Factor - metabolism</subject><issn>0883-8364</issn><issn>1071-2690</issn><issn>2327-431X</issn><issn>1543-706X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EZCTM</sourceid><sourceid>EIF</sourceid><recordid>eNqN0LFv1DAUBnALgdqjsDCDlKFioAr1e3Ych-0aXZuKk1oJKnWLHMcurnJ2iRNV_PcY5ThWJg_v9322HyHvgH4GSsvzzlIUDCiUL8gKGZY5Z3D_kqyolCyXTPBj8jrGR0oZFYhH5AhYCQXlKwLfvmbN5jaHL9k6a-ad8llthiHbOm-yYLON78P0wwxODdnN6B6cf0NeWTVE83Z_npC7y833usm3N1fX9Xqba1bJKS-0NSVXqpK95SUTFBS36dbSVh12ogNBdVcpBNACFKYPGFRohaBFz1Uv2Qn5uPQ-jeHnbOLU7lzU6W3KmzDHtkSJiKxI8NMC9RhiHI1tn0a3U-OvFmj7Zz_txeXf_ST8Yd86dzvT_6PLQtL8dD9XUavBjsprFw-sSC0cIbGzhT2bLtionfHaHNQaqgqbRkJFKQVMWv6_rt2kJhd8HWY_pej7JfoYpzAeMhwrIVPzb5hXktU</recordid><startdate>19920201</startdate><enddate>19920201</enddate><creator>Sue C. 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Psychology</topic><topic>Gels</topic><topic>Gene Expression</topic><topic>Human umbilical vein endothelial cells</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Karyotyping</topic><topic>Life Sciences &amp; Biomedicine</topic><topic>Liver Neoplasms - pathology</topic><topic>Liver Neoplasms - physiopathology</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Methods. Procedures. Technologies</topic><topic>Microscopy, Electron</topic><topic>Monoclonal antibodies</topic><topic>Mucin-1</topic><topic>RNA</topic><topic>Science &amp; Technology</topic><topic>Tumor cell line</topic><topic>Tumors</topic><topic>Vimentin - metabolism</topic><topic>von Willebrand Factor - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sue C. Heffelfinger</creatorcontrib><creatorcontrib>Hal H. Hawkins</creatorcontrib><creatorcontrib>Jim Barrish</creatorcontrib><creatorcontrib>Taylor, Linda</creatorcontrib><creatorcontrib>Darlington, Gretchen J.</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 1992</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>In Vitro Cellular &amp; Developmental Biology - Animal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sue C. Heffelfinger</au><au>Hal H. Hawkins</au><au>Jim Barrish</au><au>Taylor, Linda</au><au>Darlington, Gretchen J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SK HEP-1: A Human Cell Line of Endothelial Origin</atitle><jtitle>In Vitro Cellular &amp; Developmental Biology - Animal</jtitle><stitle>IN VITRO CELL DEV-AN</stitle><addtitle>In Vitro Cell Dev Biol</addtitle><date>1992-02-01</date><risdate>1992</risdate><volume>28A</volume><issue>2</issue><spage>136</spage><epage>142</epage><pages>136-142</pages><issn>0883-8364</issn><issn>1071-2690</issn><eissn>2327-431X</eissn><eissn>1543-706X</eissn><coden>ICDBEO</coden><abstract>SK-HEP-1 is an immortal, human cell line derived from the ascitic fluid of a patient with adenocarcinoma of the liver. We have determined that these cells are of endothelial origin. Despite the location of the tumor from which SK HEP-1 was derived, the cell line does not have properties of hepatocytes. Northern blot analysis of total cellular RNA shows no messenger RNA for the hepatic-specific proteins albumin, alpha-fibrinogen, or gamma-fibrinogen. Endothelial characteristics are seen by transmission electron microscopy. These features include numerous pinocytotic vesicles, electron dense granules consistent with Weibel-Palade bodies, and abundant intermediate filaments, identified immunocytochemically as vimentin. Cultures grown on plastic dishes grow in bundles of polygonal to spindle-shaped cells. Proteins characteristic for endothelial cells are identified by immunocytochemistry. Addition of basement membrane material (Matrigel) or type I collagen to the cultures induces these cells to organize into a tubular network.</abstract><cop>COLUMBIA</cop><pub>Tissue Culture Association, Inc</pub><pmid>1371504</pmid><doi>10.1007/bf02631017</doi><tpages>7</tpages></addata></record>
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2327-431X
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language eng
recordid cdi_pubmed_primary_1371504
source Web of Science - Science Citation Index Expanded - 1992<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; MEDLINE; SpringerNature Journals; JSTOR Archive Collection A-Z Listing
subjects Adenocarcinoma - pathology
Adenocarcinoma - physiopathology
alpha 1-Antitrypsin - genetics
Animal cells
Biological and medical sciences
Biotechnology
Cell Adhesion Molecules - metabolism
Cell Biology
Cell culture techniques
Cell Line
Cell lines
Complement C3 - genetics
Cultured cells
Developmental Biology
E-Selectin
Endothelial cells
Endothelium - pathology
Endothelium - physiopathology
Establishment of new cell lines, improvement of cultural methods, mass cultures
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Gels
Gene Expression
Human umbilical vein endothelial cells
Humans
Immunohistochemistry
Karyotyping
Life Sciences & Biomedicine
Liver Neoplasms - pathology
Liver Neoplasms - physiopathology
Membrane Glycoproteins - metabolism
Methods. Procedures. Technologies
Microscopy, Electron
Monoclonal antibodies
Mucin-1
RNA
Science & Technology
Tumor cell line
Tumors
Vimentin - metabolism
von Willebrand Factor - metabolism
title SK HEP-1: A Human Cell Line of Endothelial Origin
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