Immunosuppression in the Definitive and Intermediate Hosts of the Human Parasite Schistosoma mansoni by Release of Immunoactive Neuropeptides

Evidence supporting the concept that the parasitic trematode Schistosoma mansoni may escape immune reactions from its vertebrate (man) or invertebrate (the fresh-water snail Biomphalaria glabrata) hosts by using signal molecules it has in common with these hosts was obtained by the following experim...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1992-01, Vol.89 (2), p.778-781
Hauptverfasser: Duvaux-Miret, Odile, Stefano, George B., Smith, Eric M., Dissous, Colette, Capron, Andre
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container_title Proceedings of the National Academy of Sciences - PNAS
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creator Duvaux-Miret, Odile
Stefano, George B.
Smith, Eric M.
Dissous, Colette
Capron, Andre
description Evidence supporting the concept that the parasitic trematode Schistosoma mansoni may escape immune reactions from its vertebrate (man) or invertebrate (the fresh-water snail Biomphalaria glabrata) hosts by using signal molecules it has in common with these hosts was obtained by the following experiments. The presence of immunoactive proopiomelanocortin (POMC)-derived peptides [corticotropin (ACTH), β-endorphin] in, and their release from, S. mansoni was demonstrated. Coincubation of adult worms with human polymorphonuclear leukocytes or B. glabrata immunocytes led to the appearance of α-melanotropin (MSH) in the medium. The conclusion that this α-MSH resulted from conversion of the parasite ACTH by neutral endopeptidase 24.11 (NEP) present on these cells was supported by the fact that the α-MSH level in the medium was markedly reduced by addition of the specific NEP inhibitor phosphoramidon. This interpretation is substantiated by the fact that no conversion was observed in comparable tests with human monocytes, which exhibit no NEP activity. α-MSH has the capacity to inactivate formerly active immunocytes not only from the definitive host (man, hamster) but also from the intermediate host (B. glabrata), as determined by microscopic computer-assisted examination of conformational changes. POMC-derived peptides have been detected in B. glabrata hemolymph 2, 10, and 24 days after infection by S. mansoni miracidia. Immunocytes from infected snails were found to be inactivated, and this inactivation was prevented by antibodies directed against ACTH and α-MSH. The immunoactive β-endorphin released from S. mansoni does not appear to be subject to enzymatic conversion. Since it is active at lower concentrations, it may be used for distant signaling.
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The presence of immunoactive proopiomelanocortin (POMC)-derived peptides [corticotropin (ACTH), β-endorphin] in, and their release from, S. mansoni was demonstrated. Coincubation of adult worms with human polymorphonuclear leukocytes or B. glabrata immunocytes led to the appearance of α-melanotropin (MSH) in the medium. The conclusion that this α-MSH resulted from conversion of the parasite ACTH by neutral endopeptidase 24.11 (NEP) present on these cells was supported by the fact that the α-MSH level in the medium was markedly reduced by addition of the specific NEP inhibitor phosphoramidon. This interpretation is substantiated by the fact that no conversion was observed in comparable tests with human monocytes, which exhibit no NEP activity. α-MSH has the capacity to inactivate formerly active immunocytes not only from the definitive host (man, hamster) but also from the intermediate host (B. glabrata), as determined by microscopic computer-assisted examination of conformational changes. POMC-derived peptides have been detected in B. glabrata hemolymph 2, 10, and 24 days after infection by S. mansoni miracidia. Immunocytes from infected snails were found to be inactivated, and this inactivation was prevented by antibodies directed against ACTH and α-MSH. The immunoactive β-endorphin released from S. mansoni does not appear to be subject to enzymatic conversion. 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The presence of immunoactive proopiomelanocortin (POMC)-derived peptides [corticotropin (ACTH), β-endorphin] in, and their release from, S. mansoni was demonstrated. Coincubation of adult worms with human polymorphonuclear leukocytes or B. glabrata immunocytes led to the appearance of α-melanotropin (MSH) in the medium. The conclusion that this α-MSH resulted from conversion of the parasite ACTH by neutral endopeptidase 24.11 (NEP) present on these cells was supported by the fact that the α-MSH level in the medium was markedly reduced by addition of the specific NEP inhibitor phosphoramidon. This interpretation is substantiated by the fact that no conversion was observed in comparable tests with human monocytes, which exhibit no NEP activity. α-MSH has the capacity to inactivate formerly active immunocytes not only from the definitive host (man, hamster) but also from the intermediate host (B. glabrata), as determined by microscopic computer-assisted examination of conformational changes. POMC-derived peptides have been detected in B. glabrata hemolymph 2, 10, and 24 days after infection by S. mansoni miracidia. Immunocytes from infected snails were found to be inactivated, and this inactivation was prevented by antibodies directed against ACTH and α-MSH. The immunoactive β-endorphin released from S. mansoni does not appear to be subject to enzymatic conversion. 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The presence of immunoactive proopiomelanocortin (POMC)-derived peptides [corticotropin (ACTH), β-endorphin] in, and their release from, S. mansoni was demonstrated. Coincubation of adult worms with human polymorphonuclear leukocytes or B. glabrata immunocytes led to the appearance of α-melanotropin (MSH) in the medium. The conclusion that this α-MSH resulted from conversion of the parasite ACTH by neutral endopeptidase 24.11 (NEP) present on these cells was supported by the fact that the α-MSH level in the medium was markedly reduced by addition of the specific NEP inhibitor phosphoramidon. This interpretation is substantiated by the fact that no conversion was observed in comparable tests with human monocytes, which exhibit no NEP activity. α-MSH has the capacity to inactivate formerly active immunocytes not only from the definitive host (man, hamster) but also from the intermediate host (B. glabrata), as determined by microscopic computer-assisted examination of conformational changes. POMC-derived peptides have been detected in B. glabrata hemolymph 2, 10, and 24 days after infection by S. mansoni miracidia. Immunocytes from infected snails were found to be inactivated, and this inactivation was prevented by antibodies directed against ACTH and α-MSH. The immunoactive β-endorphin released from S. mansoni does not appear to be subject to enzymatic conversion. Since it is active at lower concentrations, it may be used for distant signaling.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1309957</pmid><doi>10.1073/pnas.89.2.778</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record>
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subjects Adrenocorticotropic Hormone - immunology
Adrenocorticotropic Hormone - metabolism
alpha-MSH - immunology
alpha-MSH - metabolism
Animals
Antibodies
beta-Endorphin - immunology
beta-Endorphin - metabolism
Biomphalaria - immunology
Biomphalaria glabrata
Cells, Cultured
Cricetinae
Hemocytes
Hemolymph
Hemolymph - metabolism
Immunity (Disease)
Immunocytes
In Vitro Techniques
Intermediate hosts
Neuropeptides - immunology
Neutrophils - immunology
Parasite hosts
Parasites
Parasitism
Schistosoma mansoni - immunology
Schistosomiasis mansoni - immunology
Snails
Suppressor Factors, Immunologic
Worms
title Immunosuppression in the Definitive and Intermediate Hosts of the Human Parasite Schistosoma mansoni by Release of Immunoactive Neuropeptides
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