Constitutive versus thermoinducible expression of heterologous proteins in Escherichia coli based on strong PR,PL promoters from phage lambda

Constitutive and thermoinducible expression plasmids based on strong PR,PL promoters from phage lambda were compared for production of TNF‐alpha and its analogs under various conditions. Much higher accumulation of TNF was obtained in a constitutive system, so the wider applicability of such systems...

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Veröffentlicht in:	Biotechnology and bioengineering 2003-07, Vol.83 (2), p.181-190
Hauptverfasser:	Menart, Viktor, Jevševar, Simona, Vilar, Mateja, Trobiš, Andreja, Pavko, Aleksander
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriophage lambda - genetics bacteriophage lambda repressor cI857 Biological and medical sciences Biotechnology Cloning, Molecular - methods constitutive expression E. coli Escherichia coli - genetics Escherichia coli - growth & development Escherichia coli - virology Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetic engineering Genetic technics Methods. Procedures. Technologies Modification of gene expression level Mutagenesis Plasmids - genetics Promoter Regions, Genetic - genetics Protein Biosynthesis Recombinant Proteins - biosynthesis Repressor Proteins - metabolism Restriction Mapping RNA, Messenger - genetics tandem promoter PR tandem promoter PR,PL thermoinducible expression TNF-alpha analogs Transcription, Genetic Tumor Necrosis Factor-alpha - genetics
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