Immunohistochemical Artifact for Nitrotyrosine in Eosinophils or Eosinophil Containing Tissue

Immunohistochemical artifacts for nitrotyrosine were investigated in eosinophils with regard to fixatives. Immunoreactivity for nitrotyrosine was revealed in separated eosinophils and in gastric mucosa fixed with periodate, lysine-paraformaldehyde (PLP). The increase in immunoreactivity by PLP was d...

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Veröffentlicht in:	Free radical research 2002-11, Vol.36 (11), p.1163-1170
Hauptverfasser:	Ogino, Keiki, Nakajima, Madoka, Kodama, Norio, Kubo, Masayuki, Kimura, Shingo, Nagase, Hirohumi, Nakamura, Hiroyuki
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, High Pressure Liquid Dithionite - pharmacology Eosinophils Eosinophils - metabolism Gastric Mucosa - cytology Humans Hydrogen Peroxide - pharmacology Immunoenzyme Techniques Immunohistochemistry Nitrogen Compounds - metabolism Nitrotyrosine Ovalbumin - metabolism Oxidants - pharmacology Oxidation-Reduction Periodic Acid - metabolism Peroxidase - metabolism Plp Fixative Tyrosine - analogs & derivatives Tyrosine - immunology Tyrosine - metabolism
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