Molecular analysis of a Saccharomyces cerevisiae mutant with improved ability to utilize xylose shows enhanced expression of proteins involved in transport, initial xylose metabolism, and the pentose phosphate pathway

Differences between the recombinant xylose-utilizing Saccharomyces cerevisiae strain TMB 3399 and the mutant strain TMB 3400, derived from TMB 3399 and displaying improved ability to utilize xylose, were investigated by using genome-wide expression analysis, physiological characterization, and bioch...

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Veröffentlicht in:	Applied and Environmental Microbiology 2003-02, Vol.69 (2), p.740-746
Hauptverfasser:	Wahlbom, C.F, Otero, R.R.C, Van Zyl, W.H, Hahn-Hagerdal, B, Jonsson, L.J
Format:	Artikel
Sprache:	eng
Schlagworte:	alcohol oxidoreductases Biological and medical sciences Biological Transport carbohydrate metabolism Culture Media Engineering and Technology enzyme activity Fundamental and applied biological sciences. Psychology gene expression Gene Expression Profiling Gene Expression Regulation, Fungal genetically engineered microorganisms glucose Industrial Biotechnology Industriell bioteknik kinases messenger RNA microarray technology microbial growth mutants Mutation Oligonucleotide Array Sequence Analysis oxidoreductases pentose phosphate cycle Pentose Phosphate Pathway physiological transport Physiology and Biotechnology RNA, Messenger - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Teknik transporters xylitol dehydrogenase xylose Xylose - metabolism xylose reductase xyulokinase yeasts
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