Block of Glutamate-Glutamine Cycle Between Astrocytes and Neurons Inhibits Epileptiform Activity in Hippocampus
1 Consiglio Nazionale delle Ricerche Institute of Neuroscience, Cellular and Molecular Pharmacology, Department of Medical Pharmacology, 20129 Milano; 2 Istituto Neurologico C. Besta, 20100 Milano; and 3 Università Degli Studi Dell'insubria, 21100 Varese, Italy Bacci, Alberto, Giulio Sanc...
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| Veröffentlicht in: | Journal of neurophysiology 2002-11, Vol.88 (5), p.2302-2310 |
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| container_title | Journal of neurophysiology |
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| creator | Bacci, Alberto Sancini, Giulio Verderio, Claudia Armano, Simona Pravettoni, Elena Fesce, Riccardo Franceschetti, Silvana Matteoli, Michela |
| description | 1 Consiglio Nazionale delle Ricerche
Institute of Neuroscience, Cellular and Molecular Pharmacology,
Department of Medical Pharmacology, 20129 Milano;
2 Istituto Neurologico C. Besta, 20100 Milano;
and 3 Università Degli Studi
Dell'insubria, 21100 Varese, Italy
Bacci, Alberto,
Giulio Sancini,
Claudia Verderio,
Simona Armano,
Elena Pravettoni,
Riccardo Fesce,
Silvana Franceschetti, and
Michela Matteoli.
Block of Glutamate-Glutamine Cycle Between Astrocytes and Neurons
Inhibits Epileptiform Activity in Hippocampus. J. Neurophysiol. 88: 2302-2310, 2002. Recurrent
epileptiform activity occurs spontaneously in cultured CNS neurons and
in brain slices in which GABA inhibition has been blocked. We
demonstrate here that pharmacological treatments resulting in either
the block of glutamine production by astrocytes or the inhibition of
glutamine uptake by neurons suppress or markedly decrease the frequency
of spontaneous epileptiform discharges both in primary hippocampal
cultures and in disinhibited hippocampal slices. These data point to an
important role for the neuron-astrocyte metabolic interaction in
sustaining episodes of intense rhythmic activity in the CNS, and
thereby reveal a new potential target for antiepileptic treatments.
*
A. Bacci and G. Sancini contributed equally to this work. |
| doi_str_mv | 10.1152/jn.00665.2001 |
| format | Article |
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Institute of Neuroscience, Cellular and Molecular Pharmacology,
Department of Medical Pharmacology, 20129 Milano;
2 Istituto Neurologico C. Besta, 20100 Milano;
and 3 Università Degli Studi
Dell'insubria, 21100 Varese, Italy
Bacci, Alberto,
Giulio Sancini,
Claudia Verderio,
Simona Armano,
Elena Pravettoni,
Riccardo Fesce,
Silvana Franceschetti, and
Michela Matteoli.
Block of Glutamate-Glutamine Cycle Between Astrocytes and Neurons
Inhibits Epileptiform Activity in Hippocampus. J. Neurophysiol. 88: 2302-2310, 2002. Recurrent
epileptiform activity occurs spontaneously in cultured CNS neurons and
in brain slices in which GABA inhibition has been blocked. We
demonstrate here that pharmacological treatments resulting in either
the block of glutamine production by astrocytes or the inhibition of
glutamine uptake by neurons suppress or markedly decrease the frequency
of spontaneous epileptiform discharges both in primary hippocampal
cultures and in disinhibited hippocampal slices. These data point to an
important role for the neuron-astrocyte metabolic interaction in
sustaining episodes of intense rhythmic activity in the CNS, and
thereby reveal a new potential target for antiepileptic treatments.
*
A. Bacci and G. Sancini contributed equally to this work.</description><identifier>ISSN: 0022-3077</identifier><identifier>EISSN: 1522-1598</identifier><identifier>DOI: 10.1152/jn.00665.2001</identifier><identifier>PMID: 12424271</identifier><language>eng</language><publisher>United States: Am Phys Soc</publisher><subject>Animals ; Astrocytes - physiology ; Cells, Cultured ; Electrophysiology ; Epilepsy - physiopathology ; Extracellular Space - physiology ; Fluorescent Dyes ; Fura-2 ; GABA Antagonists - pharmacology ; Glutamic Acid - physiology ; Glutamine - antagonists & inhibitors ; Glutamine - biosynthesis ; Glutamine - physiology ; Hippocampus - cytology ; Hippocampus - physiopathology ; Membrane Potentials - physiology ; Microscopy, Video ; Neuroglia - physiology ; Neurons - physiology ; Patch-Clamp Techniques ; Rats</subject><ispartof>Journal of neurophysiology, 2002-11, Vol.88 (5), p.2302-2310</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-a9fd65b077b8bf8ad7dd63fe85361e804f4c754a0c3cf912e83cc76efb4aadb3</citedby><cites>FETCH-LOGICAL-c462t-a9fd65b077b8bf8ad7dd63fe85361e804f4c754a0c3cf912e83cc76efb4aadb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3026,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12424271$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bacci, Alberto</creatorcontrib><creatorcontrib>Sancini, Giulio</creatorcontrib><creatorcontrib>Verderio, Claudia</creatorcontrib><creatorcontrib>Armano, Simona</creatorcontrib><creatorcontrib>Pravettoni, Elena</creatorcontrib><creatorcontrib>Fesce, Riccardo</creatorcontrib><creatorcontrib>Franceschetti, Silvana</creatorcontrib><creatorcontrib>Matteoli, Michela</creatorcontrib><title>Block of Glutamate-Glutamine Cycle Between Astrocytes and Neurons Inhibits Epileptiform Activity in Hippocampus</title><title>Journal of neurophysiology</title><addtitle>J Neurophysiol</addtitle><description> 1 Consiglio Nazionale delle Ricerche
Institute of Neuroscience, Cellular and Molecular Pharmacology,
Department of Medical Pharmacology, 20129 Milano;
2 Istituto Neurologico C. Besta, 20100 Milano;
and 3 Università Degli Studi
Dell'insubria, 21100 Varese, Italy
Bacci, Alberto,
Giulio Sancini,
Claudia Verderio,
Simona Armano,
Elena Pravettoni,
Riccardo Fesce,
Silvana Franceschetti, and
Michela Matteoli.
Block of Glutamate-Glutamine Cycle Between Astrocytes and Neurons
Inhibits Epileptiform Activity in Hippocampus. J. Neurophysiol. 88: 2302-2310, 2002. Recurrent
epileptiform activity occurs spontaneously in cultured CNS neurons and
in brain slices in which GABA inhibition has been blocked. We
demonstrate here that pharmacological treatments resulting in either
the block of glutamine production by astrocytes or the inhibition of
glutamine uptake by neurons suppress or markedly decrease the frequency
of spontaneous epileptiform discharges both in primary hippocampal
cultures and in disinhibited hippocampal slices. These data point to an
important role for the neuron-astrocyte metabolic interaction in
sustaining episodes of intense rhythmic activity in the CNS, and
thereby reveal a new potential target for antiepileptic treatments.
*
A. Bacci and G. Sancini contributed equally to this work.</description><subject>Animals</subject><subject>Astrocytes - physiology</subject><subject>Cells, Cultured</subject><subject>Electrophysiology</subject><subject>Epilepsy - physiopathology</subject><subject>Extracellular Space - physiology</subject><subject>Fluorescent Dyes</subject><subject>Fura-2</subject><subject>GABA Antagonists - pharmacology</subject><subject>Glutamic Acid - physiology</subject><subject>Glutamine - antagonists & inhibitors</subject><subject>Glutamine - biosynthesis</subject><subject>Glutamine - physiology</subject><subject>Hippocampus - cytology</subject><subject>Hippocampus - physiopathology</subject><subject>Membrane Potentials - physiology</subject><subject>Microscopy, Video</subject><subject>Neuroglia - physiology</subject><subject>Neurons - physiology</subject><subject>Patch-Clamp Techniques</subject><subject>Rats</subject><issn>0022-3077</issn><issn>1522-1598</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2P0zAQxS0EYsvCkSvyCU4pdpzY7rFb7Ze0gkvvluOMty5OHGyHJf89XlrBCaE5zGj0m6eneQi9p2RNaVt_Po5rQjhv1zUh9AValV1d0XYjX6IVIWVmRIgL9CalIyFEtKR-jS5o3ZQSdIXClQ_mGw4W3_o560FnqE6TGwHvFuMBX0F-AhjxNuUYzJIhYT32-AvMMYwJ348H17mc8PXkPEzZ2RAHvDXZ_XB5wW7Ed26agtHDNKe36JXVPsG7c79E-5vr_e6uevh6e7_bPlSm4XWu9Mb2vO2K9U52Vupe9D1nFmTLOAVJGtsY0TaaGGbshtYgmTGCg-0arfuOXaKPJ9kphu8zpKwGlwx4r0cIc1Ki5lxwQv8LUsklZ1QUsDqBJoaUIlg1RTfouChK1HMS6jiq30mo5yQK_-EsPHcD9H_p8-sLwE7AwT0enlwENR2W5IIPj4u6mb3fw89cRKVURZKRWk29LVef_n1VHPyh2S93E6ap</recordid><startdate>20021101</startdate><enddate>20021101</enddate><creator>Bacci, Alberto</creator><creator>Sancini, Giulio</creator><creator>Verderio, Claudia</creator><creator>Armano, Simona</creator><creator>Pravettoni, Elena</creator><creator>Fesce, Riccardo</creator><creator>Franceschetti, Silvana</creator><creator>Matteoli, Michela</creator><general>Am Phys Soc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20021101</creationdate><title>Block of Glutamate-Glutamine Cycle Between Astrocytes and Neurons Inhibits Epileptiform Activity in Hippocampus</title><author>Bacci, Alberto ; Sancini, Giulio ; Verderio, Claudia ; Armano, Simona ; Pravettoni, Elena ; Fesce, Riccardo ; Franceschetti, Silvana ; Matteoli, Michela</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-a9fd65b077b8bf8ad7dd63fe85361e804f4c754a0c3cf912e83cc76efb4aadb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Astrocytes - physiology</topic><topic>Cells, Cultured</topic><topic>Electrophysiology</topic><topic>Epilepsy - physiopathology</topic><topic>Extracellular Space - physiology</topic><topic>Fluorescent Dyes</topic><topic>Fura-2</topic><topic>GABA Antagonists - pharmacology</topic><topic>Glutamic Acid - physiology</topic><topic>Glutamine - antagonists & inhibitors</topic><topic>Glutamine - biosynthesis</topic><topic>Glutamine - physiology</topic><topic>Hippocampus - cytology</topic><topic>Hippocampus - physiopathology</topic><topic>Membrane Potentials - physiology</topic><topic>Microscopy, Video</topic><topic>Neuroglia - physiology</topic><topic>Neurons - physiology</topic><topic>Patch-Clamp Techniques</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bacci, Alberto</creatorcontrib><creatorcontrib>Sancini, Giulio</creatorcontrib><creatorcontrib>Verderio, Claudia</creatorcontrib><creatorcontrib>Armano, Simona</creatorcontrib><creatorcontrib>Pravettoni, Elena</creatorcontrib><creatorcontrib>Fesce, Riccardo</creatorcontrib><creatorcontrib>Franceschetti, Silvana</creatorcontrib><creatorcontrib>Matteoli, Michela</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurophysiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bacci, Alberto</au><au>Sancini, Giulio</au><au>Verderio, Claudia</au><au>Armano, Simona</au><au>Pravettoni, Elena</au><au>Fesce, Riccardo</au><au>Franceschetti, Silvana</au><au>Matteoli, Michela</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Block of Glutamate-Glutamine Cycle Between Astrocytes and Neurons Inhibits Epileptiform Activity in Hippocampus</atitle><jtitle>Journal of neurophysiology</jtitle><addtitle>J Neurophysiol</addtitle><date>2002-11-01</date><risdate>2002</risdate><volume>88</volume><issue>5</issue><spage>2302</spage><epage>2310</epage><pages>2302-2310</pages><issn>0022-3077</issn><eissn>1522-1598</eissn><abstract> 1 Consiglio Nazionale delle Ricerche
Institute of Neuroscience, Cellular and Molecular Pharmacology,
Department of Medical Pharmacology, 20129 Milano;
2 Istituto Neurologico C. Besta, 20100 Milano;
and 3 Università Degli Studi
Dell'insubria, 21100 Varese, Italy
Bacci, Alberto,
Giulio Sancini,
Claudia Verderio,
Simona Armano,
Elena Pravettoni,
Riccardo Fesce,
Silvana Franceschetti, and
Michela Matteoli.
Block of Glutamate-Glutamine Cycle Between Astrocytes and Neurons
Inhibits Epileptiform Activity in Hippocampus. J. Neurophysiol. 88: 2302-2310, 2002. Recurrent
epileptiform activity occurs spontaneously in cultured CNS neurons and
in brain slices in which GABA inhibition has been blocked. We
demonstrate here that pharmacological treatments resulting in either
the block of glutamine production by astrocytes or the inhibition of
glutamine uptake by neurons suppress or markedly decrease the frequency
of spontaneous epileptiform discharges both in primary hippocampal
cultures and in disinhibited hippocampal slices. These data point to an
important role for the neuron-astrocyte metabolic interaction in
sustaining episodes of intense rhythmic activity in the CNS, and
thereby reveal a new potential target for antiepileptic treatments.
*
A. Bacci and G. Sancini contributed equally to this work.</abstract><cop>United States</cop><pub>Am Phys Soc</pub><pmid>12424271</pmid><doi>10.1152/jn.00665.2001</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of neurophysiology, 2002-11, Vol.88 (5), p.2302-2310 |
| issn | 0022-3077 1522-1598 |
| language | eng |
| recordid | cdi_pubmed_primary_12424271 |
| source | MEDLINE; American Physiological Society Paid; EZB Free E-Journals |
| subjects | Animals Astrocytes - physiology Cells, Cultured Electrophysiology Epilepsy - physiopathology Extracellular Space - physiology Fluorescent Dyes Fura-2 GABA Antagonists - pharmacology Glutamic Acid - physiology Glutamine - antagonists & inhibitors Glutamine - biosynthesis Glutamine - physiology Hippocampus - cytology Hippocampus - physiopathology Membrane Potentials - physiology Microscopy, Video Neuroglia - physiology Neurons - physiology Patch-Clamp Techniques Rats |
| title | Block of Glutamate-Glutamine Cycle Between Astrocytes and Neurons Inhibits Epileptiform Activity in Hippocampus |
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