Influence of spleen on cell cycle and expression of proliferating cell nuclear antigen of the liver during diethylnitrosamine-induced rat hepatocarcinogenesis
To investigate the role of the spleen on hepatocyte proliferative activity during experimental hepatocarcinogenesis in rats. Cell-cycle and expression of proliferating cell nuclear antigen (PCNA) of hepatocytes were studied by immunohistochemical and flow cytometric technique in two groups: splenect...
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| Veröffentlicht in: | Chung-hua wai kʿo tsa chih 2002-08, Vol.40 (8), p.613 |
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| creator | Wang, Guanyu Chen, Xiaoping Qiu, Fazu Tao, Deding |
| description | To investigate the role of the spleen on hepatocyte proliferative activity during experimental hepatocarcinogenesis in rats.
Cell-cycle and expression of proliferating cell nuclear antigen (PCNA) of hepatocytes were studied by immunohistochemical and flow cytometric technique in two groups: splenectomy (45 rats) and sham-operation with laparotomy (45 rats).
(1) Hepatocirrhosis was formed in group A earlier than in group B. Marked degenerative changes of the liver parachyma showing vacuolization of hepatocytes were observed in hepatic lobules. (2) The proliferative level of hepatocytes increased with the progression of hepatocarcinogenesis, and topped at the 18 th week. (3) The proliferative level of the splenectomy group was lower than that of the sham-operation group in the mid-stage of carcinogenesis (t = 4.76, P < 0.05). After stopping feeding diethylnitrosamine (DENA), however, no difference was found in the hepatocyte proliferative activity between the two groups at the 18th week.
There is a close relati |
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Cell-cycle and expression of proliferating cell nuclear antigen (PCNA) of hepatocytes were studied by immunohistochemical and flow cytometric technique in two groups: splenectomy (45 rats) and sham-operation with laparotomy (45 rats).
(1) Hepatocirrhosis was formed in group A earlier than in group B. Marked degenerative changes of the liver parachyma showing vacuolization of hepatocytes were observed in hepatic lobules. (2) The proliferative level of hepatocytes increased with the progression of hepatocarcinogenesis, and topped at the 18 th week. (3) The proliferative level of the splenectomy group was lower than that of the sham-operation group in the mid-stage of carcinogenesis (t = 4.76, P < 0.05). After stopping feeding diethylnitrosamine (DENA), however, no difference was found in the hepatocyte proliferative activity between the two groups at the 18th week.
There is a close relati</description><identifier>ISSN: 0529-5815</identifier><identifier>PMID: 12417078</identifier><language>chi</language><publisher>China</publisher><subject>Animals ; Cell Cycle ; Diethylnitrosamine ; Liver - pathology ; Liver Neoplasms, Experimental - chemically induced ; Liver Neoplasms, Experimental - pathology ; Male ; Proliferating Cell Nuclear Antigen - analysis ; Rats ; Rats, Wistar ; Spleen - physiology ; Splenectomy</subject><ispartof>Chung-hua wai kʿo tsa chih, 2002-08, Vol.40 (8), p.613</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12417078$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Guanyu</creatorcontrib><creatorcontrib>Chen, Xiaoping</creatorcontrib><creatorcontrib>Qiu, Fazu</creatorcontrib><creatorcontrib>Tao, Deding</creatorcontrib><title>Influence of spleen on cell cycle and expression of proliferating cell nuclear antigen of the liver during diethylnitrosamine-induced rat hepatocarcinogenesis</title><title>Chung-hua wai kʿo tsa chih</title><addtitle>Zhonghua Wai Ke Za Zhi</addtitle><description>To investigate the role of the spleen on hepatocyte proliferative activity during experimental hepatocarcinogenesis in rats.
Cell-cycle and expression of proliferating cell nuclear antigen (PCNA) of hepatocytes were studied by immunohistochemical and flow cytometric technique in two groups: splenectomy (45 rats) and sham-operation with laparotomy (45 rats).
(1) Hepatocirrhosis was formed in group A earlier than in group B. Marked degenerative changes of the liver parachyma showing vacuolization of hepatocytes were observed in hepatic lobules. (2) The proliferative level of hepatocytes increased with the progression of hepatocarcinogenesis, and topped at the 18 th week. (3) The proliferative level of the splenectomy group was lower than that of the sham-operation group in the mid-stage of carcinogenesis (t = 4.76, P < 0.05). After stopping feeding diethylnitrosamine (DENA), however, no difference was found in the hepatocyte proliferative activity between the two groups at the 18th week.
There is a close relati</description><subject>Animals</subject><subject>Cell Cycle</subject><subject>Diethylnitrosamine</subject><subject>Liver - pathology</subject><subject>Liver Neoplasms, Experimental - chemically induced</subject><subject>Liver Neoplasms, Experimental - pathology</subject><subject>Male</subject><subject>Proliferating Cell Nuclear Antigen - analysis</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Spleen - physiology</subject><subject>Splenectomy</subject><issn>0529-5815</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo10M1OAyEUhmEWGlurt2C4gUkGhvlbmsafJk3c6Lo5wKHFUIYAY-zNeK2i1dVZfE_exbkgy7rlY9UOrF2Q65Te61rwuu2uyIJxwfq6H5bka-ONm9ErpJOhKThETydPFTpH1Uk5pOA1xc8QMSVblsJCnJw1GCFbvz9TPxcKseBs9_ir8gGpsx8YqZ7jD9QW8-HkvM1xSnC0Hivr9axQ05KiBwyQJwVRWT-VBiabbsilAZfw9u-uyNvjw-v6udq-PG3W99sqMM5zZYQW0GjsmlEJJnvGa1Cml2zQ3WigLytIwQcpeYMceSdaI5XqG8FNB6NoVuTu3A2zPKLehWiPEE-7_0c13w9UaUs</recordid><startdate>200208</startdate><enddate>200208</enddate><creator>Wang, Guanyu</creator><creator>Chen, Xiaoping</creator><creator>Qiu, Fazu</creator><creator>Tao, Deding</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>200208</creationdate><title>Influence of spleen on cell cycle and expression of proliferating cell nuclear antigen of the liver during diethylnitrosamine-induced rat hepatocarcinogenesis</title><author>Wang, Guanyu ; Chen, Xiaoping ; Qiu, Fazu ; Tao, Deding</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p122t-f4d4a3de639c41b7120acf7b18d69fa7d4aab428bb23e2e2645fbcc7342f6a943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Cell Cycle</topic><topic>Diethylnitrosamine</topic><topic>Liver - pathology</topic><topic>Liver Neoplasms, Experimental - chemically induced</topic><topic>Liver Neoplasms, Experimental - pathology</topic><topic>Male</topic><topic>Proliferating Cell Nuclear Antigen - analysis</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Spleen - physiology</topic><topic>Splenectomy</topic><toplevel>online_resources</toplevel><creatorcontrib>Wang, Guanyu</creatorcontrib><creatorcontrib>Chen, Xiaoping</creatorcontrib><creatorcontrib>Qiu, Fazu</creatorcontrib><creatorcontrib>Tao, Deding</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Chung-hua wai kʿo tsa chih</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Guanyu</au><au>Chen, Xiaoping</au><au>Qiu, Fazu</au><au>Tao, Deding</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of spleen on cell cycle and expression of proliferating cell nuclear antigen of the liver during diethylnitrosamine-induced rat hepatocarcinogenesis</atitle><jtitle>Chung-hua wai kʿo tsa chih</jtitle><addtitle>Zhonghua Wai Ke Za Zhi</addtitle><date>2002-08</date><risdate>2002</risdate><volume>40</volume><issue>8</issue><spage>613</spage><pages>613-</pages><issn>0529-5815</issn><abstract>To investigate the role of the spleen on hepatocyte proliferative activity during experimental hepatocarcinogenesis in rats.
Cell-cycle and expression of proliferating cell nuclear antigen (PCNA) of hepatocytes were studied by immunohistochemical and flow cytometric technique in two groups: splenectomy (45 rats) and sham-operation with laparotomy (45 rats).
(1) Hepatocirrhosis was formed in group A earlier than in group B. Marked degenerative changes of the liver parachyma showing vacuolization of hepatocytes were observed in hepatic lobules. (2) The proliferative level of hepatocytes increased with the progression of hepatocarcinogenesis, and topped at the 18 th week. (3) The proliferative level of the splenectomy group was lower than that of the sham-operation group in the mid-stage of carcinogenesis (t = 4.76, P < 0.05). After stopping feeding diethylnitrosamine (DENA), however, no difference was found in the hepatocyte proliferative activity between the two groups at the 18th week.
There is a close relati</abstract><cop>China</cop><pmid>12417078</pmid></addata></record> |
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| ispartof | Chung-hua wai kʿo tsa chih, 2002-08, Vol.40 (8), p.613 |
| issn | 0529-5815 |
| language | chi |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Cell Cycle Diethylnitrosamine Liver - pathology Liver Neoplasms, Experimental - chemically induced Liver Neoplasms, Experimental - pathology Male Proliferating Cell Nuclear Antigen - analysis Rats Rats, Wistar Spleen - physiology Splenectomy |
| title | Influence of spleen on cell cycle and expression of proliferating cell nuclear antigen of the liver during diethylnitrosamine-induced rat hepatocarcinogenesis |
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