Electron Microscopy Analysis of Early Localization of Cisplatin in Ovarian Carcinoma Cells
Taking advantage of the electron-dense nature of platinum, in this study the authors used an electron microscopy approach to investigate the cellular localization of cisplatin in an ovarian carcinoma cell line. Platinum spots were detected in contact with the plasma membrane and the nuclear envelope...
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| Veröffentlicht in: | Ultrastructural pathology 2002-09, Vol.26 (5), p.331-334 |
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| container_issue | 5 |
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| container_title | Ultrastructural pathology |
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| creator | Beretta, Giovanni L. Righetti, Sabina C. Lombardi, Luciano Zunino, Franco Perego, Paola |
| description | Taking advantage of the electron-dense nature of platinum, in this study the authors used an electron microscopy approach to investigate the cellular localization of cisplatin in an ovarian carcinoma cell line. Platinum spots were detected in contact with the plasma membrane and the nuclear envelope as well as in the cytoplasm and nuclear matrices. Contact with the plasma membrane was through a single blunt contact or spanning through the membrane. No sequestration in intracellular vescicles was observed, thereby supporting that phagocytosis and receptor-mediated endocytosis were not occurring. A molecular analysis indicated lack of expression of aquaporin 9, thus excluding its involvement in the membrane translocation of cisplatin. The present data suggest that cisplatin rapidly accumulates in the cell through endocytosis-independent membrane translocation and are consistent with passive diffusion. |
| doi_str_mv | 10.1080/01913120290104610 |
| format | Article |
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Platinum spots were detected in contact with the plasma membrane and the nuclear envelope as well as in the cytoplasm and nuclear matrices. Contact with the plasma membrane was through a single blunt contact or spanning through the membrane. No sequestration in intracellular vescicles was observed, thereby supporting that phagocytosis and receptor-mediated endocytosis were not occurring. A molecular analysis indicated lack of expression of aquaporin 9, thus excluding its involvement in the membrane translocation of cisplatin. The present data suggest that cisplatin rapidly accumulates in the cell through endocytosis-independent membrane translocation and are consistent with passive diffusion.</description><identifier>ISSN: 0191-3123</identifier><identifier>EISSN: 1521-0758</identifier><identifier>DOI: 10.1080/01913120290104610</identifier><identifier>PMID: 12396244</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Aquaporins - metabolism ; Carcinoma - metabolism ; Carcinoma - ultrastructure ; Cell Membrane - metabolism ; Cell Membrane - ultrastructure ; Cell Nucleus - metabolism ; Cell Nucleus - ultrastructure ; Cisplatin ; Cisplatin - analysis ; Cisplatin - metabolism ; Female ; Humans ; Localization ; Microscopy, Electron ; Ovarian Carcinoma Cells ; Ovarian Neoplasms - metabolism ; Ovarian Neoplasms - ultrastructure ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Neoplasm - analysis ; Tumor Cells, Cultured</subject><ispartof>Ultrastructural pathology, 2002-09, Vol.26 (5), p.331-334</ispartof><rights>2002 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-d30d3e5de8949a35a0feae49f6890af8f32c8f3f9e8e303666eaa7d0f63feba73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/01913120290104610$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/01913120290104610$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,59647,59753,60436,60542,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12396244$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Beretta, Giovanni L.</creatorcontrib><creatorcontrib>Righetti, Sabina C.</creatorcontrib><creatorcontrib>Lombardi, Luciano</creatorcontrib><creatorcontrib>Zunino, Franco</creatorcontrib><creatorcontrib>Perego, Paola</creatorcontrib><title>Electron Microscopy Analysis of Early Localization of Cisplatin in Ovarian Carcinoma Cells</title><title>Ultrastructural pathology</title><addtitle>Ultrastruct Pathol</addtitle><description>Taking advantage of the electron-dense nature of platinum, in this study the authors used an electron microscopy approach to investigate the cellular localization of cisplatin in an ovarian carcinoma cell line. Platinum spots were detected in contact with the plasma membrane and the nuclear envelope as well as in the cytoplasm and nuclear matrices. Contact with the plasma membrane was through a single blunt contact or spanning through the membrane. No sequestration in intracellular vescicles was observed, thereby supporting that phagocytosis and receptor-mediated endocytosis were not occurring. A molecular analysis indicated lack of expression of aquaporin 9, thus excluding its involvement in the membrane translocation of cisplatin. The present data suggest that cisplatin rapidly accumulates in the cell through endocytosis-independent membrane translocation and are consistent with passive diffusion.</description><subject>Aquaporins - metabolism</subject><subject>Carcinoma - metabolism</subject><subject>Carcinoma - ultrastructure</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Cisplatin</subject><subject>Cisplatin - analysis</subject><subject>Cisplatin - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>Localization</subject><subject>Microscopy, Electron</subject><subject>Ovarian Carcinoma Cells</subject><subject>Ovarian Neoplasms - metabolism</subject><subject>Ovarian Neoplasms - ultrastructure</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Neoplasm - analysis</subject><subject>Tumor Cells, Cultured</subject><issn>0191-3123</issn><issn>1521-0758</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9r3DAQxUVpaDZpP0AuwafenIwsWyuTXoLZ_IENubSXXsREHhEF2dpK3hbn01fLLpRQCAiJGf3eY_TE2BmHCw4KLoG3XPAKqhY41JLDB7bgTcVLWDbqI1vs7ssMiGN2ktILADQC1Cd2nFutrOp6wX6uPJkphrF4cCaGZMJmLq5H9HNyqQi2WGH0c7EOBr17xcllMnc7lzY-V2OR1-NvjA7HosNo3BgGLDryPn1mRxZ9oi-H85T9uFl97-7K9ePtfXe9Lk0t1VT2AnpBTU-qrVsUDYIlpLq1UrWAVllRmbzZlhQJEFJKQlz2YKWw9IRLccq-7n03MfzaUpr04JLJE-BIYZv0spJcVgoyyPfg7qEpktWb6AaMs-agd4Hq_wLNmvOD-fZpoP6f4pBgBr7tATfaEAf8E6Lv9YSzD9FGHI1LWrznf_VG_kzop2eDkfRL2Mb8Eemd6f4CLUGWdw</recordid><startdate>20020901</startdate><enddate>20020901</enddate><creator>Beretta, Giovanni L.</creator><creator>Righetti, Sabina C.</creator><creator>Lombardi, Luciano</creator><creator>Zunino, Franco</creator><creator>Perego, Paola</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020901</creationdate><title>Electron Microscopy Analysis of Early Localization of Cisplatin in Ovarian Carcinoma Cells</title><author>Beretta, Giovanni L. ; Righetti, Sabina C. ; Lombardi, Luciano ; Zunino, Franco ; Perego, Paola</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-d30d3e5de8949a35a0feae49f6890af8f32c8f3f9e8e303666eaa7d0f63feba73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Aquaporins - metabolism</topic><topic>Carcinoma - metabolism</topic><topic>Carcinoma - ultrastructure</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Membrane - ultrastructure</topic><topic>Cell Nucleus - metabolism</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Cisplatin</topic><topic>Cisplatin - analysis</topic><topic>Cisplatin - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>Localization</topic><topic>Microscopy, Electron</topic><topic>Ovarian Carcinoma Cells</topic><topic>Ovarian Neoplasms - metabolism</topic><topic>Ovarian Neoplasms - ultrastructure</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Neoplasm - analysis</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Beretta, Giovanni L.</creatorcontrib><creatorcontrib>Righetti, Sabina C.</creatorcontrib><creatorcontrib>Lombardi, Luciano</creatorcontrib><creatorcontrib>Zunino, Franco</creatorcontrib><creatorcontrib>Perego, Paola</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Ultrastructural pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Beretta, Giovanni L.</au><au>Righetti, Sabina C.</au><au>Lombardi, Luciano</au><au>Zunino, Franco</au><au>Perego, Paola</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electron Microscopy Analysis of Early Localization of Cisplatin in Ovarian Carcinoma Cells</atitle><jtitle>Ultrastructural pathology</jtitle><addtitle>Ultrastruct Pathol</addtitle><date>2002-09-01</date><risdate>2002</risdate><volume>26</volume><issue>5</issue><spage>331</spage><epage>334</epage><pages>331-334</pages><issn>0191-3123</issn><eissn>1521-0758</eissn><abstract>Taking advantage of the electron-dense nature of platinum, in this study the authors used an electron microscopy approach to investigate the cellular localization of cisplatin in an ovarian carcinoma cell line. Platinum spots were detected in contact with the plasma membrane and the nuclear envelope as well as in the cytoplasm and nuclear matrices. Contact with the plasma membrane was through a single blunt contact or spanning through the membrane. No sequestration in intracellular vescicles was observed, thereby supporting that phagocytosis and receptor-mediated endocytosis were not occurring. A molecular analysis indicated lack of expression of aquaporin 9, thus excluding its involvement in the membrane translocation of cisplatin. The present data suggest that cisplatin rapidly accumulates in the cell through endocytosis-independent membrane translocation and are consistent with passive diffusion.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>12396244</pmid><doi>10.1080/01913120290104610</doi><tpages>4</tpages></addata></record> |
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| ispartof | Ultrastructural pathology, 2002-09, Vol.26 (5), p.331-334 |
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| language | eng |
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| subjects | Aquaporins - metabolism Carcinoma - metabolism Carcinoma - ultrastructure Cell Membrane - metabolism Cell Membrane - ultrastructure Cell Nucleus - metabolism Cell Nucleus - ultrastructure Cisplatin Cisplatin - analysis Cisplatin - metabolism Female Humans Localization Microscopy, Electron Ovarian Carcinoma Cells Ovarian Neoplasms - metabolism Ovarian Neoplasms - ultrastructure Reverse Transcriptase Polymerase Chain Reaction RNA, Neoplasm - analysis Tumor Cells, Cultured |
| title | Electron Microscopy Analysis of Early Localization of Cisplatin in Ovarian Carcinoma Cells |
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