Effects of Elevated Pressure on Prostanoid Receptor Gene Expression Levels in Human Trabecular Meshwork
The purpose of this study was to assess the effects of increased intraocular pressure on the expression levels of the prostanoid receptor genes (DP, EP 1–4 , FP, IP, TP) in the trabecular meshwork of human donor eyes. Anterior segments of human donor eyes were perfused in an ex vivo anterior segment...
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| Veröffentlicht in: | Ophthalmic research 2002-09, Vol.34 (5), p.314-318 |
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| creator | Schneemann, Andrea Hoyng, Philip F.J. Kamphuis, Willem |
| description | The purpose of this study was to assess the effects of increased intraocular pressure on the expression levels of the prostanoid receptor genes (DP, EP 1–4 , FP, IP, TP) in the trabecular meshwork of human donor eyes. Anterior segments of human donor eyes were perfused in an ex vivo anterior segment perfusion system under different pressure regimes. The expression levels of the prostanoid receptors and of several housekeeping genes were assessed by non-competitive real-time quantitative polymerase chain reaction (Q-PCR). Perfusion of the anterior segments for 24 h at 10 mm Hg, followed by 24 h at 30 mm Hg, caused a significant decrease in the expression of the EP 2 receptor compared to a constant perfusion under 10 mm Hg. No significant changes were found for the other prostanoid receptor transcripts. When the pressure was raised to 30 mm Hg for only 1 or 3 h, no changes in the EP 2 receptor expression levels were evident. However, a transient DP and TP receptor expression increase was found after 1-hour perfusion at 30 mm Hg. Whether these changes in expression are part of a response of the trabecular meshwork cells in order to normalise intraocular pressure needs to be studied further. |
| doi_str_mv | 10.1159/000065606 |
| format | Article |
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Anterior segments of human donor eyes were perfused in an ex vivo anterior segment perfusion system under different pressure regimes. The expression levels of the prostanoid receptors and of several housekeeping genes were assessed by non-competitive real-time quantitative polymerase chain reaction (Q-PCR). Perfusion of the anterior segments for 24 h at 10 mm Hg, followed by 24 h at 30 mm Hg, caused a significant decrease in the expression of the EP 2 receptor compared to a constant perfusion under 10 mm Hg. No significant changes were found for the other prostanoid receptor transcripts. When the pressure was raised to 30 mm Hg for only 1 or 3 h, no changes in the EP 2 receptor expression levels were evident. However, a transient DP and TP receptor expression increase was found after 1-hour perfusion at 30 mm Hg. Whether these changes in expression are part of a response of the trabecular meshwork cells in order to normalise intraocular pressure needs to be studied further.</description><identifier>ISSN: 0030-3747</identifier><identifier>EISSN: 1423-0259</identifier><identifier>DOI: 10.1159/000065606</identifier><identifier>PMID: 12381894</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Anterior Eye Segment - physiology ; Computer Systems ; Gene Expression - physiology ; Humans ; Intraocular Pressure - physiology ; Original Paper ; Polymerase Chain Reaction - methods ; Receptors, Immunologic ; Receptors, Prostaglandin - genetics ; Receptors, Prostaglandin - metabolism ; Receptors, Prostaglandin E - metabolism ; Receptors, Prostaglandin E, EP2 Subtype ; Receptors, Thromboxane - metabolism ; Time Factors ; Trabecular Meshwork - physiology</subject><ispartof>Ophthalmic research, 2002-09, Vol.34 (5), p.314-318</ispartof><rights>2002 S. 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Anterior segments of human donor eyes were perfused in an ex vivo anterior segment perfusion system under different pressure regimes. The expression levels of the prostanoid receptors and of several housekeeping genes were assessed by non-competitive real-time quantitative polymerase chain reaction (Q-PCR). Perfusion of the anterior segments for 24 h at 10 mm Hg, followed by 24 h at 30 mm Hg, caused a significant decrease in the expression of the EP 2 receptor compared to a constant perfusion under 10 mm Hg. No significant changes were found for the other prostanoid receptor transcripts. When the pressure was raised to 30 mm Hg for only 1 or 3 h, no changes in the EP 2 receptor expression levels were evident. However, a transient DP and TP receptor expression increase was found after 1-hour perfusion at 30 mm Hg. Whether these changes in expression are part of a response of the trabecular meshwork cells in order to normalise intraocular pressure needs to be studied further.</description><subject>Anterior Eye Segment - physiology</subject><subject>Computer Systems</subject><subject>Gene Expression - physiology</subject><subject>Humans</subject><subject>Intraocular Pressure - physiology</subject><subject>Original Paper</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Receptors, Immunologic</subject><subject>Receptors, Prostaglandin - genetics</subject><subject>Receptors, Prostaglandin - metabolism</subject><subject>Receptors, Prostaglandin E - metabolism</subject><subject>Receptors, Prostaglandin E, EP2 Subtype</subject><subject>Receptors, Thromboxane - metabolism</subject><subject>Time Factors</subject><subject>Trabecular Meshwork - physiology</subject><issn>0030-3747</issn><issn>1423-0259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqF0U1LHTEUBuBQKvXWduG6IMGF0MXYnGQymSxFxg-4RRG7HjKTEx2dO5kmM378-8bei0IRmk2yeHgPOS8hu8AOAaT-wdIpZMGKD2QBORcZ41J_JAvGBMuEytU2-RzjHWMJa_aJbAMXJZQ6X5Cbyjlsp0i9o1WPD2ZCSy8DxjgHpH5Ibx8nM_jO0itscZx8oKc4IK2exhfWJbPEB-wj7QZ6Nq_MQK-DabCdexPoT4y3jz7cfyFbzvQRv27uHfLrpLo-PsuWF6fnx0fLrBVlMWVKOi2UVaWy0GqZW5kXijdN6axWjVNQcpm3oIChQRQWdM4MNDrnDULhUOyQg3XuGPzvGeNUr7rYYt-bAf0ca8WhBAnqv5ADZ1wXOsH9f-Cdn8OQPlFzzkAwyURC39eoTeuKAV09hm5lwnMNrH7pqH7tKNm9TeDcrNC-yU0pbxPvTbjB8Aourqq_CfVoXULf3kXrGX8AkUCfdg</recordid><startdate>20020901</startdate><enddate>20020901</enddate><creator>Schneemann, Andrea</creator><creator>Hoyng, Philip F.J.</creator><creator>Kamphuis, Willem</creator><general>S. 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physiology</topic><topic>Computer Systems</topic><topic>Gene Expression - physiology</topic><topic>Humans</topic><topic>Intraocular Pressure - physiology</topic><topic>Original Paper</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Receptors, Immunologic</topic><topic>Receptors, Prostaglandin - genetics</topic><topic>Receptors, Prostaglandin - metabolism</topic><topic>Receptors, Prostaglandin E - metabolism</topic><topic>Receptors, Prostaglandin E, EP2 Subtype</topic><topic>Receptors, Thromboxane - metabolism</topic><topic>Time Factors</topic><topic>Trabecular Meshwork - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schneemann, Andrea</creatorcontrib><creatorcontrib>Hoyng, Philip F.J.</creatorcontrib><creatorcontrib>Kamphuis, Willem</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Ophthalmic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schneemann, Andrea</au><au>Hoyng, Philip F.J.</au><au>Kamphuis, Willem</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Elevated Pressure on Prostanoid Receptor Gene Expression Levels in Human Trabecular Meshwork</atitle><jtitle>Ophthalmic research</jtitle><addtitle>Ophthalmic Res</addtitle><date>2002-09-01</date><risdate>2002</risdate><volume>34</volume><issue>5</issue><spage>314</spage><epage>318</epage><pages>314-318</pages><issn>0030-3747</issn><eissn>1423-0259</eissn><abstract>The purpose of this study was to assess the effects of increased intraocular pressure on the expression levels of the prostanoid receptor genes (DP, EP 1–4 , FP, IP, TP) in the trabecular meshwork of human donor eyes. Anterior segments of human donor eyes were perfused in an ex vivo anterior segment perfusion system under different pressure regimes. The expression levels of the prostanoid receptors and of several housekeeping genes were assessed by non-competitive real-time quantitative polymerase chain reaction (Q-PCR). Perfusion of the anterior segments for 24 h at 10 mm Hg, followed by 24 h at 30 mm Hg, caused a significant decrease in the expression of the EP 2 receptor compared to a constant perfusion under 10 mm Hg. No significant changes were found for the other prostanoid receptor transcripts. When the pressure was raised to 30 mm Hg for only 1 or 3 h, no changes in the EP 2 receptor expression levels were evident. However, a transient DP and TP receptor expression increase was found after 1-hour perfusion at 30 mm Hg. Whether these changes in expression are part of a response of the trabecular meshwork cells in order to normalise intraocular pressure needs to be studied further.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>12381894</pmid><doi>10.1159/000065606</doi><tpages>5</tpages></addata></record> |
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| subjects | Anterior Eye Segment - physiology Computer Systems Gene Expression - physiology Humans Intraocular Pressure - physiology Original Paper Polymerase Chain Reaction - methods Receptors, Immunologic Receptors, Prostaglandin - genetics Receptors, Prostaglandin - metabolism Receptors, Prostaglandin E - metabolism Receptors, Prostaglandin E, EP2 Subtype Receptors, Thromboxane - metabolism Time Factors Trabecular Meshwork - physiology |
| title | Effects of Elevated Pressure on Prostanoid Receptor Gene Expression Levels in Human Trabecular Meshwork |
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