Evaluation of tissue and cellular biomarkers to assess 2,4,6-trinitrotoluene (TNT) exposure in earthworms: effects-based assessment in laboratory studies using Eisenia andrei
The lysosomal neutral red retention time (NRRT) assay, a biomarker for lysosomal membrane stability, and the total immune activity (TIA) assay, a measure of non-specific immune system activity, were used in laboratory studies to assess the toxic effects of 2,4,6-trinitrotoluene (TNT) on earthworms (...
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| Veröffentlicht in: | Biomarkers 2002-07, Vol.7 (4), p.306-321 |
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| creator | Robidoux, Pierre Yves Svendsen, Claus Sarrazin, Manon Hawari, Jalal Thiboutot, Sonia Ampleman, Guy Weeks, Jason M. Sunahara, Geoffrey I. |
| description | The lysosomal neutral red retention time (NRRT) assay, a biomarker for lysosomal membrane stability, and the total immune activity (TIA) assay, a measure of non-specific immune system activity, were used in laboratory studies to assess the toxic effects of 2,4,6-trinitrotoluene (TNT) on earthworms (Eisenia andrei) in vivo. The results were compared with the concentration of TNT and its metabolites in earthworm tissue, as well as standard sublethal toxicity endpoints including growth (i.e. weight change) and reproduction effects from previously published studies. Filter paper experiments indicated a significant decrease in NRRT at ≥1.8 μg TNT cm-2, whereas sublethal (weight loss) and lethal effects to earthworms were detected at ≥3.5 and 7.1 μg TNT cm-2, respectively. Experiments in artificial soil showed that NRRT effects could be detected at lower TNT concentrations (≥55 mg TNT kg-1 soil dry weight) compared with other sublethal endpoints (effects on growth and reproduction). The TIA biomarker did not significantly respond to TNT. Copper (as CuSO4, filter paper contact tests) and 2-chloroacetamide (soil tests), which were used as reference toxicants, also decreased the NRRT. The use of the NRRT assay linked with tissue concentrations of TNT metabolites in earthworms was identified as a potentially appropriate biomarker approach for TNT exposure assessment under laboratory conditions and a novel tool for effects-based risk assessment. |
| doi_str_mv | 10.1080/13547500210127327 |
| format | Article |
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The results were compared with the concentration of TNT and its metabolites in earthworm tissue, as well as standard sublethal toxicity endpoints including growth (i.e. weight change) and reproduction effects from previously published studies. Filter paper experiments indicated a significant decrease in NRRT at ≥1.8 μg TNT cm-2, whereas sublethal (weight loss) and lethal effects to earthworms were detected at ≥3.5 and 7.1 μg TNT cm-2, respectively. Experiments in artificial soil showed that NRRT effects could be detected at lower TNT concentrations (≥55 mg TNT kg-1 soil dry weight) compared with other sublethal endpoints (effects on growth and reproduction). The TIA biomarker did not significantly respond to TNT. Copper (as CuSO4, filter paper contact tests) and 2-chloroacetamide (soil tests), which were used as reference toxicants, also decreased the NRRT. The use of the NRRT assay linked with tissue concentrations of TNT metabolites in earthworms was identified as a potentially appropriate biomarker approach for TNT exposure assessment under laboratory conditions and a novel tool for effects-based risk assessment.</description><identifier>ISSN: 1354-750X</identifier><identifier>EISSN: 1366-5804</identifier><identifier>DOI: 10.1080/13547500210127327</identifier><identifier>PMID: 12171758</identifier><language>eng</language><publisher>London: Informa UK Ltd</publisher><subject>Animal, plant and microbial ecology ; Animals ; Applied ecology ; Biological and medical sciences ; Biomarker ; Biomarkers - analysis ; Contaminated Soil ; Ecotoxicology, biological effects of pollution ; Eisenia andrei ; Energetic Substance ; Environmental Monitoring ; Explosives ; Fundamental and applied biological sciences. Psychology ; Immune System - drug effects ; Lysosomes - metabolism ; Neutral Red ; Oligochaeta - drug effects ; Oligochaeta - immunology ; Oligochaeta - metabolism ; Polynitroaromatic Compound ; Risk Assessment ; Soil Pollutants - pharmacokinetics ; Soil Pollutants - toxicity ; Techniques ; Tnt ; Trinitrotoluene - pharmacokinetics ; Trinitrotoluene - toxicity</subject><ispartof>Biomarkers, 2002-07, Vol.7 (4), p.306-321</ispartof><rights>2002 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2002</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c527t-7e96e26b290aa2b97165241b5b372aae457e72b38c03478d0642c89416b392a93</citedby><cites>FETCH-LOGICAL-c527t-7e96e26b290aa2b97165241b5b372aae457e72b38c03478d0642c89416b392a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/13547500210127327$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/13547500210127327$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,59647,59753,60436,60542,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13840791$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12171758$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Robidoux, Pierre Yves</creatorcontrib><creatorcontrib>Svendsen, Claus</creatorcontrib><creatorcontrib>Sarrazin, Manon</creatorcontrib><creatorcontrib>Hawari, Jalal</creatorcontrib><creatorcontrib>Thiboutot, Sonia</creatorcontrib><creatorcontrib>Ampleman, Guy</creatorcontrib><creatorcontrib>Weeks, Jason M.</creatorcontrib><creatorcontrib>Sunahara, Geoffrey I.</creatorcontrib><title>Evaluation of tissue and cellular biomarkers to assess 2,4,6-trinitrotoluene (TNT) exposure in earthworms: effects-based assessment in laboratory studies using Eisenia andrei</title><title>Biomarkers</title><addtitle>Biomarkers</addtitle><description>The lysosomal neutral red retention time (NRRT) assay, a biomarker for lysosomal membrane stability, and the total immune activity (TIA) assay, a measure of non-specific immune system activity, were used in laboratory studies to assess the toxic effects of 2,4,6-trinitrotoluene (TNT) on earthworms (Eisenia andrei) in vivo. The results were compared with the concentration of TNT and its metabolites in earthworm tissue, as well as standard sublethal toxicity endpoints including growth (i.e. weight change) and reproduction effects from previously published studies. Filter paper experiments indicated a significant decrease in NRRT at ≥1.8 μg TNT cm-2, whereas sublethal (weight loss) and lethal effects to earthworms were detected at ≥3.5 and 7.1 μg TNT cm-2, respectively. Experiments in artificial soil showed that NRRT effects could be detected at lower TNT concentrations (≥55 mg TNT kg-1 soil dry weight) compared with other sublethal endpoints (effects on growth and reproduction). The TIA biomarker did not significantly respond to TNT. Copper (as CuSO4, filter paper contact tests) and 2-chloroacetamide (soil tests), which were used as reference toxicants, also decreased the NRRT. The use of the NRRT assay linked with tissue concentrations of TNT metabolites in earthworms was identified as a potentially appropriate biomarker approach for TNT exposure assessment under laboratory conditions and a novel tool for effects-based risk assessment.</description><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Applied ecology</subject><subject>Biological and medical sciences</subject><subject>Biomarker</subject><subject>Biomarkers - analysis</subject><subject>Contaminated Soil</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Eisenia andrei</subject><subject>Energetic Substance</subject><subject>Environmental Monitoring</subject><subject>Explosives</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immune System - drug effects</subject><subject>Lysosomes - metabolism</subject><subject>Neutral Red</subject><subject>Oligochaeta - drug effects</subject><subject>Oligochaeta - immunology</subject><subject>Oligochaeta - metabolism</subject><subject>Polynitroaromatic Compound</subject><subject>Risk Assessment</subject><subject>Soil Pollutants - pharmacokinetics</subject><subject>Soil Pollutants - toxicity</subject><subject>Techniques</subject><subject>Tnt</subject><subject>Trinitrotoluene - pharmacokinetics</subject><subject>Trinitrotoluene - toxicity</subject><issn>1354-750X</issn><issn>1366-5804</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc2O1DAQhCMEYpeFB-CCfAGBtAH_JHECXNBq-JFWcBkkblEn6TBeHHtw28C8FM9IoglaIaQ9tQ9fVbersuyh4M8Fr_kLocpCl5xLwYXUSupb2alQVZWXNS9uL--yyGfgy0l2j-iKc6FkU9_NToQUWuiyPs1-b36ATRCNd8yPLBqihAzcwHq0NlkIrDN-gvANA7HoGRAhEZPnxXmVx2CcicFHbxM6ZE-3H7fPGP7ae0oBmXEMIcTdTx8meslwHLGPlHdAOKxGE7q4cBY6HyD6cGAU02CQWCLjvrKNIXQGlpMCmvvZnREs4YN1nmWf3262F-_zy0_vPly8ucz7UuqYa2wqlFUnGw4gu0aLqpSF6MpOaQmARalRy07VPVeFrgdeFbKvm0JUnWokNOose3L03Qf_PSHFdjK0JAIOfaJWaM2l1mIGxRHsgycKOLb7YOa4Dq3g7VJS-19Js-bRap66CYdrxdrKDDxeAaAe7BjA9YauOVUXXDfL8tdHzrhxjhjmnO3QRjhYH_6K1E13vPpHvkOwcddDwPbKp-DmgG_4xR9qwsD8</recordid><startdate>20020701</startdate><enddate>20020701</enddate><creator>Robidoux, Pierre Yves</creator><creator>Svendsen, Claus</creator><creator>Sarrazin, Manon</creator><creator>Hawari, Jalal</creator><creator>Thiboutot, Sonia</creator><creator>Ampleman, Guy</creator><creator>Weeks, Jason M.</creator><creator>Sunahara, Geoffrey I.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20020701</creationdate><title>Evaluation of tissue and cellular biomarkers to assess 2,4,6-trinitrotoluene (TNT) exposure in earthworms: effects-based assessment in laboratory studies using Eisenia andrei</title><author>Robidoux, Pierre Yves ; Svendsen, Claus ; Sarrazin, Manon ; Hawari, Jalal ; Thiboutot, Sonia ; Ampleman, Guy ; Weeks, Jason M. ; Sunahara, Geoffrey I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c527t-7e96e26b290aa2b97165241b5b372aae457e72b38c03478d0642c89416b392a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Applied ecology</topic><topic>Biological and medical sciences</topic><topic>Biomarker</topic><topic>Biomarkers - analysis</topic><topic>Contaminated Soil</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Eisenia andrei</topic><topic>Energetic Substance</topic><topic>Environmental Monitoring</topic><topic>Explosives</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immune System - drug effects</topic><topic>Lysosomes - metabolism</topic><topic>Neutral Red</topic><topic>Oligochaeta - drug effects</topic><topic>Oligochaeta - immunology</topic><topic>Oligochaeta - metabolism</topic><topic>Polynitroaromatic Compound</topic><topic>Risk Assessment</topic><topic>Soil Pollutants - pharmacokinetics</topic><topic>Soil Pollutants - toxicity</topic><topic>Techniques</topic><topic>Tnt</topic><topic>Trinitrotoluene - pharmacokinetics</topic><topic>Trinitrotoluene - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Robidoux, Pierre Yves</creatorcontrib><creatorcontrib>Svendsen, Claus</creatorcontrib><creatorcontrib>Sarrazin, Manon</creatorcontrib><creatorcontrib>Hawari, Jalal</creatorcontrib><creatorcontrib>Thiboutot, Sonia</creatorcontrib><creatorcontrib>Ampleman, Guy</creatorcontrib><creatorcontrib>Weeks, Jason M.</creatorcontrib><creatorcontrib>Sunahara, Geoffrey I.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Biomarkers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Robidoux, Pierre Yves</au><au>Svendsen, Claus</au><au>Sarrazin, Manon</au><au>Hawari, Jalal</au><au>Thiboutot, Sonia</au><au>Ampleman, Guy</au><au>Weeks, Jason M.</au><au>Sunahara, Geoffrey I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of tissue and cellular biomarkers to assess 2,4,6-trinitrotoluene (TNT) exposure in earthworms: effects-based assessment in laboratory studies using Eisenia andrei</atitle><jtitle>Biomarkers</jtitle><addtitle>Biomarkers</addtitle><date>2002-07-01</date><risdate>2002</risdate><volume>7</volume><issue>4</issue><spage>306</spage><epage>321</epage><pages>306-321</pages><issn>1354-750X</issn><eissn>1366-5804</eissn><abstract>The lysosomal neutral red retention time (NRRT) assay, a biomarker for lysosomal membrane stability, and the total immune activity (TIA) assay, a measure of non-specific immune system activity, were used in laboratory studies to assess the toxic effects of 2,4,6-trinitrotoluene (TNT) on earthworms (Eisenia andrei) in vivo. The results were compared with the concentration of TNT and its metabolites in earthworm tissue, as well as standard sublethal toxicity endpoints including growth (i.e. weight change) and reproduction effects from previously published studies. Filter paper experiments indicated a significant decrease in NRRT at ≥1.8 μg TNT cm-2, whereas sublethal (weight loss) and lethal effects to earthworms were detected at ≥3.5 and 7.1 μg TNT cm-2, respectively. Experiments in artificial soil showed that NRRT effects could be detected at lower TNT concentrations (≥55 mg TNT kg-1 soil dry weight) compared with other sublethal endpoints (effects on growth and reproduction). The TIA biomarker did not significantly respond to TNT. Copper (as CuSO4, filter paper contact tests) and 2-chloroacetamide (soil tests), which were used as reference toxicants, also decreased the NRRT. The use of the NRRT assay linked with tissue concentrations of TNT metabolites in earthworms was identified as a potentially appropriate biomarker approach for TNT exposure assessment under laboratory conditions and a novel tool for effects-based risk assessment.</abstract><cop>London</cop><pub>Informa UK Ltd</pub><pmid>12171758</pmid><doi>10.1080/13547500210127327</doi><tpages>16</tpages></addata></record> |
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| ispartof | Biomarkers, 2002-07, Vol.7 (4), p.306-321 |
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| subjects | Animal, plant and microbial ecology Animals Applied ecology Biological and medical sciences Biomarker Biomarkers - analysis Contaminated Soil Ecotoxicology, biological effects of pollution Eisenia andrei Energetic Substance Environmental Monitoring Explosives Fundamental and applied biological sciences. Psychology Immune System - drug effects Lysosomes - metabolism Neutral Red Oligochaeta - drug effects Oligochaeta - immunology Oligochaeta - metabolism Polynitroaromatic Compound Risk Assessment Soil Pollutants - pharmacokinetics Soil Pollutants - toxicity Techniques Tnt Trinitrotoluene - pharmacokinetics Trinitrotoluene - toxicity |
| title | Evaluation of tissue and cellular biomarkers to assess 2,4,6-trinitrotoluene (TNT) exposure in earthworms: effects-based assessment in laboratory studies using Eisenia andrei |
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