Cloning and characterization of extracellular metal protease gene of the algicidal marine bacterium Pseudoalteromonas sp. strain A28
The gene (empI) encoding an extracellular metal protease was isolated from a Pseudoalteromonas sp. strain A28 DNA library. The recombinant EmpI protein was expressed in E. coli and purified. Paper-disk assays showed that the purified protease had potent algicidal activity. A skim milk-polyacrylamide...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2002-06, Vol.66 (6), p.1366-1369 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Lee, S.O. (Hiroshima Univ. (Japan)) Kato, J Nakashima, K Kuroda, A Ikeda, T Takiguchi, N Ohtake, H |
| description | The gene (empI) encoding an extracellular metal protease was isolated from a Pseudoalteromonas sp. strain A28 DNA library. The recombinant EmpI protein was expressed in E. coli and purified. Paper-disk assays showed that the purified protease had potent algicidal activity. A skim milk-polyacrylamide gel electrophoresis protease assay showed that the 38-kDa band of protease activity, which co-migrated with purified EmpI and was sensitive to 1,10-phenathroline, was detected in the extracellular supernatant of A28. |
| doi_str_mv | 10.1271/bbb.66.1366 |
| format | Article |
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(Hiroshima Univ. (Japan)) ; Kato, J ; Nakashima, K ; Kuroda, A ; Ikeda, T ; Takiguchi, N ; Ohtake, H</creator><creatorcontrib>Lee, S.O. (Hiroshima Univ. (Japan)) ; Kato, J ; Nakashima, K ; Kuroda, A ; Ikeda, T ; Takiguchi, N ; Ohtake, H</creatorcontrib><description>The gene (empI) encoding an extracellular metal protease was isolated from a Pseudoalteromonas sp. strain A28 DNA library. The recombinant EmpI protein was expressed in E. coli and purified. Paper-disk assays showed that the purified protease had potent algicidal activity. A skim milk-polyacrylamide gel electrophoresis protease assay showed that the 38-kDa band of protease activity, which co-migrated with purified EmpI and was sensitive to 1,10-phenathroline, was detected in the extracellular supernatant of A28.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.66.1366</identifier><identifier>PMID: 12162559</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>1,10-phenanthroline ; algicidal marine bacterium ; ALGICIDES ; BACTERIA ; Biological and medical sciences ; Biological control and other methods ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; Cloning, Molecular ; Electrophoresis, Polyacrylamide Gel ; empI gene ; Endopeptidases - chemistry ; Endopeptidases - genetics ; Endopeptidases - isolation & purification ; Endopeptidases - metabolism ; extracellular metal protease ; Fundamental and applied biological sciences. Psychology ; Metals - metabolism ; Miscellaneous ; Mission oriented research ; MOLECULAR CLONING ; Molecular Weight ; Parasitic plants. Weeds ; Phytopathology. Animal pests. Plant and forest protection ; PROTEASES ; Pseudoalteromonas ; Pseudoalteromonas - enzymology ; Pseudoalteromonas - genetics ; red tide algae ; Restriction Mapping ; Weeds</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2002-06, Vol.66 (6), p.1366-1369</ispartof><rights>2002 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2002</rights><rights>2002 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c569t-ad7cfc1ea912fce3cb0758f626e783a93f1d4a3484df45e828197520d54bb0383</citedby><cites>FETCH-LOGICAL-c569t-ad7cfc1ea912fce3cb0758f626e783a93f1d4a3484df45e828197520d54bb0383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13807109$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12162559$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, S.O. (Hiroshima Univ. (Japan))</creatorcontrib><creatorcontrib>Kato, J</creatorcontrib><creatorcontrib>Nakashima, K</creatorcontrib><creatorcontrib>Kuroda, A</creatorcontrib><creatorcontrib>Ikeda, T</creatorcontrib><creatorcontrib>Takiguchi, N</creatorcontrib><creatorcontrib>Ohtake, H</creatorcontrib><title>Cloning and characterization of extracellular metal protease gene of the algicidal marine bacterium Pseudoalteromonas sp. strain A28</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>The gene (empI) encoding an extracellular metal protease was isolated from a Pseudoalteromonas sp. strain A28 DNA library. The recombinant EmpI protein was expressed in E. coli and purified. Paper-disk assays showed that the purified protease had potent algicidal activity. A skim milk-polyacrylamide gel electrophoresis protease assay showed that the 38-kDa band of protease activity, which co-migrated with purified EmpI and was sensitive to 1,10-phenathroline, was detected in the extracellular supernatant of A28.</description><subject>1,10-phenanthroline</subject><subject>algicidal marine bacterium</subject><subject>ALGICIDES</subject><subject>BACTERIA</subject><subject>Biological and medical sciences</subject><subject>Biological control and other methods</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>Cloning, Molecular</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>empI gene</subject><subject>Endopeptidases - chemistry</subject><subject>Endopeptidases - genetics</subject><subject>Endopeptidases - isolation & purification</subject><subject>Endopeptidases - metabolism</subject><subject>extracellular metal protease</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Metals - metabolism</subject><subject>Miscellaneous</subject><subject>Mission oriented research</subject><subject>MOLECULAR CLONING</subject><subject>Molecular Weight</subject><subject>Parasitic plants. Weeds</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>PROTEASES</subject><subject>Pseudoalteromonas</subject><subject>Pseudoalteromonas - enzymology</subject><subject>Pseudoalteromonas - genetics</subject><subject>red tide algae</subject><subject>Restriction Mapping</subject><subject>Weeds</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UuLFDEQAOAgijuunjwrAdGLzJhXJ93HZfDJgnvQc6hOJ7NZ0smYdKPr2R9umh5ZEEEIhFS-FFUphJ5SsqNM0Td93--k3FEu5T20oVyoreyEuo82pKNy24qGnqFHpdwQUgMNfYjOKKOSNU23Qb_2IUUfDxjigM01ZDCTzf4nTD5FnBy2P6YasyHMATIe7QQBH3OaLBSLDzbaBU3XFkM4eOOHej1C9jXer6nmEV8VOw8JQj2mMUUouBx3uNTEPuIL1j5GDxyEYp-c9nP09d3bL_sP28vP7z_uLy63ppHdtIVBGWeohY4yZyw3PVFN6ySTVrUcOu7oIICLVgxONLZlLe1Uw8jQiL4nvOXn6NWatzbwbbZl0qMvS28QbZqLVosXjPwX0lYSqjir8MVf8CbNOdYmNBWiE0JJIqp6vSqTUynZOn3Mvv7SraZELzPUdYZaSr3MsOrnp5xzP9rhzp6GVsHLE4BiILgM0fhy53hLFCWLk6vz0aU8wveUw6AnuA0p_3nE_13Bs_Whg6ThkKv7dMUIqatWwPlv4bzAVw</recordid><startdate>20020601</startdate><enddate>20020601</enddate><creator>Lee, S.O. 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(Hiroshima Univ. (Japan)) ; Kato, J ; Nakashima, K ; Kuroda, A ; Ikeda, T ; Takiguchi, N ; Ohtake, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c569t-ad7cfc1ea912fce3cb0758f626e783a93f1d4a3484df45e828197520d54bb0383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>1,10-phenanthroline</topic><topic>algicidal marine bacterium</topic><topic>ALGICIDES</topic><topic>BACTERIA</topic><topic>Biological and medical sciences</topic><topic>Biological control and other methods</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>Cloning, Molecular</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>empI gene</topic><topic>Endopeptidases - chemistry</topic><topic>Endopeptidases - genetics</topic><topic>Endopeptidases - isolation & purification</topic><topic>Endopeptidases - metabolism</topic><topic>extracellular metal protease</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Metals - metabolism</topic><topic>Miscellaneous</topic><topic>Mission oriented research</topic><topic>MOLECULAR CLONING</topic><topic>Molecular Weight</topic><topic>Parasitic plants. Weeds</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>PROTEASES</topic><topic>Pseudoalteromonas</topic><topic>Pseudoalteromonas - enzymology</topic><topic>Pseudoalteromonas - genetics</topic><topic>red tide algae</topic><topic>Restriction Mapping</topic><topic>Weeds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, S.O. (Hiroshima Univ. 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(Hiroshima Univ. (Japan))</au><au>Kato, J</au><au>Nakashima, K</au><au>Kuroda, A</au><au>Ikeda, T</au><au>Takiguchi, N</au><au>Ohtake, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and characterization of extracellular metal protease gene of the algicidal marine bacterium Pseudoalteromonas sp. strain A28</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2002-06-01</date><risdate>2002</risdate><volume>66</volume><issue>6</issue><spage>1366</spage><epage>1369</epage><pages>1366-1369</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>The gene (empI) encoding an extracellular metal protease was isolated from a Pseudoalteromonas sp. strain A28 DNA library. The recombinant EmpI protein was expressed in E. coli and purified. Paper-disk assays showed that the purified protease had potent algicidal activity. A skim milk-polyacrylamide gel electrophoresis protease assay showed that the 38-kDa band of protease activity, which co-migrated with purified EmpI and was sensitive to 1,10-phenathroline, was detected in the extracellular supernatant of A28.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>12162559</pmid><doi>10.1271/bbb.66.1366</doi><tpages>4</tpages></addata></record> |
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| source | J-STAGE Free; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | 1,10-phenanthroline algicidal marine bacterium ALGICIDES BACTERIA Biological and medical sciences Biological control and other methods Biology of microorganisms of confirmed or potential industrial interest Biotechnology Cloning, Molecular Electrophoresis, Polyacrylamide Gel empI gene Endopeptidases - chemistry Endopeptidases - genetics Endopeptidases - isolation & purification Endopeptidases - metabolism extracellular metal protease Fundamental and applied biological sciences. Psychology Metals - metabolism Miscellaneous Mission oriented research MOLECULAR CLONING Molecular Weight Parasitic plants. Weeds Phytopathology. Animal pests. Plant and forest protection PROTEASES Pseudoalteromonas Pseudoalteromonas - enzymology Pseudoalteromonas - genetics red tide algae Restriction Mapping Weeds |
| title | Cloning and characterization of extracellular metal protease gene of the algicidal marine bacterium Pseudoalteromonas sp. strain A28 |
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