A Crucial Role for the Putative Arabidopsis Topoisomerase VI in Plant Growth and Development
Plant steroid hormones, brassinosteroids (BRs), play important roles throughout plant growth and development. Plants defective in BR biosynthesis or perception display cell elongation defects and severe dwarfism. Two dwarf mutants named bin3 and bin5 with identical phenotypes to each other display s...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2002-07, Vol.99 (15), p.10191-10196 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Yin, Yanhai Cheong, Hyeonsook Friedrichsen, Danielle Zhao, Yunde Hu, Jianping Mora-Garcia, Santiago Chory, Joanne |
| description | Plant steroid hormones, brassinosteroids (BRs), play important roles throughout plant growth and development. Plants defective in BR biosynthesis or perception display cell elongation defects and severe dwarfism. Two dwarf mutants named bin3 and bin5 with identical phenotypes to each other display some characteristics of BR mutants and are partially insensitive to exogenously applied BRs. In the dark, bin3 or bin5 seedlings are de-etiolated with short hypocotyls and open cotyledons. Light-grown mutant plants are dwarfs with short petioles, epinastic leaves, short inflorescence stems, and reduced apical dominance. We cloned BIN3 and BIN5 and show that BIN5 is one of three putative Arabidopsis SPO11 homologs (AtSPO11-3) that also shares significant homology to archaebacterial topoisomerase VI (TOP6) subunit A, whereas BIN3 represents a putative eukaryotic homolog of TOP6B. The pleiotropic dwarf phenotypes of bin5 establish that, unlike all of the other SPO11 homologs that are involved in meiosis, BIN5/AtSPO11-3 plays a major role during somatic development. Furthermore, microarray analysis of the expression of about 5500 genes in bin3 or bin5 mutants indicates that about 321 genes are down-regulated in both of the mutants, including 18 of 30 BR-induced genes. These results suggest that BIN3 and BIN5 may constitute an Arabidopsis topoisomerase VI that modulates expression of many genes, including those regulated by BRs. |
| doi_str_mv | 10.1073/pnas.152337599 |
| format | Article |
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Plants defective in BR biosynthesis or perception display cell elongation defects and severe dwarfism. Two dwarf mutants named bin3 and bin5 with identical phenotypes to each other display some characteristics of BR mutants and are partially insensitive to exogenously applied BRs. In the dark, bin3 or bin5 seedlings are de-etiolated with short hypocotyls and open cotyledons. Light-grown mutant plants are dwarfs with short petioles, epinastic leaves, short inflorescence stems, and reduced apical dominance. We cloned BIN3 and BIN5 and show that BIN5 is one of three putative Arabidopsis SPO11 homologs (AtSPO11-3) that also shares significant homology to archaebacterial topoisomerase VI (TOP6) subunit A, whereas BIN3 represents a putative eukaryotic homolog of TOP6B. The pleiotropic dwarf phenotypes of bin5 establish that, unlike all of the other SPO11 homologs that are involved in meiosis, BIN5/AtSPO11-3 plays a major role during somatic development. Furthermore, microarray analysis of the expression of about 5500 genes in bin3 or bin5 mutants indicates that about 321 genes are down-regulated in both of the mutants, including 18 of 30 BR-induced genes. These results suggest that BIN3 and BIN5 may constitute an Arabidopsis topoisomerase VI that modulates expression of many genes, including those regulated by BRs.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.152337599</identifier><identifier>PMID: 12119417</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Amino Acid Sequence ; Arabidopsis - enzymology ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Archaeal Proteins ; Biological Sciences ; Carrier Proteins - chemistry ; Carrier Proteins - genetics ; Cell growth ; Developmental biology ; DNA Topoisomerases, Type II - genetics ; DNA Topoisomerases, Type II - metabolism ; Drosophila Proteins ; Flowers & plants ; Gene expression regulation ; Genes ; Genetic mutation ; Hormones ; Hypocotyls ; Insect Proteins - chemistry ; Insect Proteins - genetics ; Molecular Sequence Data ; Mutation ; Oligonucleotide Array Sequence Analysis ; Phenotypes ; Phylogeny ; Phytosterols - metabolism ; Plant development ; Plant growth ; Plants ; Seedlings ; Transcription Factors</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2002-07, Vol.99 (15), p.10191-10196</ispartof><rights>Copyright 1993-2002 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Jul 23, 2002</rights><rights>Copyright © 2002, The National Academy of Sciences 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-c8d9a1db1824278a8bbf874f586bd3d45b8688518385f4baeda0b8a991a1ee833</citedby><cites>FETCH-LOGICAL-c521t-c8d9a1db1824278a8bbf874f586bd3d45b8688518385f4baeda0b8a991a1ee833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/99/15.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3059356$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3059356$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12119417$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yin, Yanhai</creatorcontrib><creatorcontrib>Cheong, Hyeonsook</creatorcontrib><creatorcontrib>Friedrichsen, Danielle</creatorcontrib><creatorcontrib>Zhao, Yunde</creatorcontrib><creatorcontrib>Hu, Jianping</creatorcontrib><creatorcontrib>Mora-Garcia, Santiago</creatorcontrib><creatorcontrib>Chory, Joanne</creatorcontrib><title>A Crucial Role for the Putative Arabidopsis Topoisomerase VI in Plant Growth and Development</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Plant steroid hormones, brassinosteroids (BRs), play important roles throughout plant growth and development. Plants defective in BR biosynthesis or perception display cell elongation defects and severe dwarfism. Two dwarf mutants named bin3 and bin5 with identical phenotypes to each other display some characteristics of BR mutants and are partially insensitive to exogenously applied BRs. In the dark, bin3 or bin5 seedlings are de-etiolated with short hypocotyls and open cotyledons. Light-grown mutant plants are dwarfs with short petioles, epinastic leaves, short inflorescence stems, and reduced apical dominance. We cloned BIN3 and BIN5 and show that BIN5 is one of three putative Arabidopsis SPO11 homologs (AtSPO11-3) that also shares significant homology to archaebacterial topoisomerase VI (TOP6) subunit A, whereas BIN3 represents a putative eukaryotic homolog of TOP6B. The pleiotropic dwarf phenotypes of bin5 establish that, unlike all of the other SPO11 homologs that are involved in meiosis, BIN5/AtSPO11-3 plays a major role during somatic development. Furthermore, microarray analysis of the expression of about 5500 genes in bin3 or bin5 mutants indicates that about 321 genes are down-regulated in both of the mutants, including 18 of 30 BR-induced genes. These results suggest that BIN3 and BIN5 may constitute an Arabidopsis topoisomerase VI that modulates expression of many genes, including those regulated by BRs.</description><subject>Amino Acid Sequence</subject><subject>Arabidopsis - enzymology</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - growth & development</subject><subject>Archaeal Proteins</subject><subject>Biological Sciences</subject><subject>Carrier Proteins - chemistry</subject><subject>Carrier Proteins - genetics</subject><subject>Cell growth</subject><subject>Developmental biology</subject><subject>DNA Topoisomerases, Type II - genetics</subject><subject>DNA Topoisomerases, Type II - metabolism</subject><subject>Drosophila Proteins</subject><subject>Flowers & plants</subject><subject>Gene expression regulation</subject><subject>Genes</subject><subject>Genetic mutation</subject><subject>Hormones</subject><subject>Hypocotyls</subject><subject>Insect Proteins - chemistry</subject><subject>Insect Proteins - genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Phenotypes</subject><subject>Phylogeny</subject><subject>Phytosterols - metabolism</subject><subject>Plant development</subject><subject>Plant growth</subject><subject>Plants</subject><subject>Seedlings</subject><subject>Transcription Factors</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuP0zAUhSMEYsrAlhUCiwVik-LrR2wvWFQdGEYaiREaWCFZTuJQV2mcsZ0y_HtcWspjAau7uN8593GK4jHgOWBBX42DiXPghFLBlbpTzAArKCum8N1ihjERpWSEnRQPYlxjjBWX-H5xAgRAMRCz4vMCLcPUONOjD763qPMBpZVFV1MyyW0tWgRTu9aP0UV07Ufvot_YYKJFny6QG9BVb4aEzoP_mlbIDC06s1vb-3Fjh_SwuNeZPtpHh3pafHz75nr5rrx8f36xXFyWDSeQyka2ykBbgySMCGlkXXdSsI7Lqm5py3gtKyk5SCp5x2pjW4NraZQCA9ZKSk-L13vfcao3tm3y6GB6PQa3MeGb9sbpPzuDW-kvfquBVBWrsv7FQR_8zWRj0hsXG9vn06yfohb5WYIJ_F8QJKNSVCyDz_8C134KQ36CJhgYJeKH23wPNcHHGGx33Biw3qWrd-nqY7pZ8PT3O3_hhzgz8OwA7IQ_20plj2wICjLx8t-E7qa-T_Y2ZfTJHl3H5MORpZgryiv6HXG8wpQ</recordid><startdate>20020723</startdate><enddate>20020723</enddate><creator>Yin, Yanhai</creator><creator>Cheong, Hyeonsook</creator><creator>Friedrichsen, Danielle</creator><creator>Zhao, Yunde</creator><creator>Hu, Jianping</creator><creator>Mora-Garcia, Santiago</creator><creator>Chory, Joanne</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><general>The National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20020723</creationdate><title>A Crucial Role for the Putative Arabidopsis Topoisomerase VI in Plant Growth and Development</title><author>Yin, Yanhai ; 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Plants defective in BR biosynthesis or perception display cell elongation defects and severe dwarfism. Two dwarf mutants named bin3 and bin5 with identical phenotypes to each other display some characteristics of BR mutants and are partially insensitive to exogenously applied BRs. In the dark, bin3 or bin5 seedlings are de-etiolated with short hypocotyls and open cotyledons. Light-grown mutant plants are dwarfs with short petioles, epinastic leaves, short inflorescence stems, and reduced apical dominance. We cloned BIN3 and BIN5 and show that BIN5 is one of three putative Arabidopsis SPO11 homologs (AtSPO11-3) that also shares significant homology to archaebacterial topoisomerase VI (TOP6) subunit A, whereas BIN3 represents a putative eukaryotic homolog of TOP6B. The pleiotropic dwarf phenotypes of bin5 establish that, unlike all of the other SPO11 homologs that are involved in meiosis, BIN5/AtSPO11-3 plays a major role during somatic development. Furthermore, microarray analysis of the expression of about 5500 genes in bin3 or bin5 mutants indicates that about 321 genes are down-regulated in both of the mutants, including 18 of 30 BR-induced genes. These results suggest that BIN3 and BIN5 may constitute an Arabidopsis topoisomerase VI that modulates expression of many genes, including those regulated by BRs.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>12119417</pmid><doi>10.1073/pnas.152337599</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis - growth & development Archaeal Proteins Biological Sciences Carrier Proteins - chemistry Carrier Proteins - genetics Cell growth Developmental biology DNA Topoisomerases, Type II - genetics DNA Topoisomerases, Type II - metabolism Drosophila Proteins Flowers & plants Gene expression regulation Genes Genetic mutation Hormones Hypocotyls Insect Proteins - chemistry Insect Proteins - genetics Molecular Sequence Data Mutation Oligonucleotide Array Sequence Analysis Phenotypes Phylogeny Phytosterols - metabolism Plant development Plant growth Plants Seedlings Transcription Factors |
| title | A Crucial Role for the Putative Arabidopsis Topoisomerase VI in Plant Growth and Development |
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