Observation of Molecular Changes of a Necrotic Tissue from a Murine Carcinoma by Fourier-Transform Infrared Microspectroscopy
Purpose: Our purpose is to develop infrared (IR) microspectroscopy as a new optical diagnostic tool to support conventional lightscopic techniques in investigating the viability of carcinoma tissues and to develop its use in the evaluation of the early effects of anticancer therapy by monitoring the...
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| Veröffentlicht in: | Clinical cancer research 2002-06, Vol.8 (6), p.2010-2014 |
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| container_issue | 6 |
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| container_title | Clinical cancer research |
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| creator | YAMADA, Tetsushi MIYOSHI, Norio OGAWA, Toru AKAO, Kenichi FUKUDA, Masaru OGASAWARA, Toshiyuki KITAGAWA, Yoshimasa SANO, Kazuo |
| description | Purpose: Our purpose is to develop infrared (IR) microspectroscopy as a new optical diagnostic tool to support conventional lightscopic
techniques in investigating the viability of carcinoma tissues and to develop its use in the evaluation of the early effects
of anticancer therapy by monitoring the IR spectra in the necrotic area.
Experimental Design: We evaluated the tissue which amassed for 4 weeks after the isotransplantation of mouse squamous cell carcinoma into the
thigh of mice. The borders of the necrotic area of frozen tissue specimens were investigated by Fourier-transform IR microspectroscopy
and conventional histological staining.
Results: A significantly higher accumulation of cholesterol was observed in the necrotic tissue of a carcinoma. The mechanism of this
phenomenon is hitherto unrecognized. We proposed that the accumulated cholesterol may lie extracellularly as a result of the
ruptured plasma and internal membranes after the swelling of the necrotic cells brought on by hypoxia. The analysis of the
secondary structure of protein revealed that the amounts of β-sheet increased significantly in striking contrast to the decreasing
amounts of α-helix in a necrotic area of a carcinoma. It is plausible that this structural conversion of protein was because
of lipid-autooxidation products, such as cholesterol oxide but not cholesterol itself, which possesses cell toxicity and could
be generated in a necrotic area.
Conclusions: We conclude that it will be possible to evaluate the efficacy of the clinical treatment of carcinoma by monitoring subtle
biological changes of cholesterol absorbance in the early stage of necrosis because of anticancer treatment. |
| format | Article |
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techniques in investigating the viability of carcinoma tissues and to develop its use in the evaluation of the early effects
of anticancer therapy by monitoring the IR spectra in the necrotic area.
Experimental Design: We evaluated the tissue which amassed for 4 weeks after the isotransplantation of mouse squamous cell carcinoma into the
thigh of mice. The borders of the necrotic area of frozen tissue specimens were investigated by Fourier-transform IR microspectroscopy
and conventional histological staining.
Results: A significantly higher accumulation of cholesterol was observed in the necrotic tissue of a carcinoma. The mechanism of this
phenomenon is hitherto unrecognized. We proposed that the accumulated cholesterol may lie extracellularly as a result of the
ruptured plasma and internal membranes after the swelling of the necrotic cells brought on by hypoxia. The analysis of the
secondary structure of protein revealed that the amounts of β-sheet increased significantly in striking contrast to the decreasing
amounts of α-helix in a necrotic area of a carcinoma. It is plausible that this structural conversion of protein was because
of lipid-autooxidation products, such as cholesterol oxide but not cholesterol itself, which possesses cell toxicity and could
be generated in a necrotic area.
Conclusions: We conclude that it will be possible to evaluate the efficacy of the clinical treatment of carcinoma by monitoring subtle
biological changes of cholesterol absorbance in the early stage of necrosis because of anticancer treatment.</description><identifier>ISSN: 1078-0432</identifier><identifier>EISSN: 1557-3265</identifier><identifier>PMID: 12060647</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Animals ; Antineoplastic agents ; Azo Compounds ; Biological and medical sciences ; Carcinoma, Squamous Cell - pathology ; Chemotherapy ; Cholesterol - metabolism ; Male ; Medical sciences ; Mice ; Mice, Inbred C3H ; Necrosis ; Pharmacology. Drug treatments ; Protein Conformation ; Soft Tissue Neoplasms - pathology ; Spectroscopy, Fourier Transform Infrared - methods</subject><ispartof>Clinical cancer research, 2002-06, Vol.8 (6), p.2010-2014</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13700508$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12060647$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YAMADA, Tetsushi</creatorcontrib><creatorcontrib>MIYOSHI, Norio</creatorcontrib><creatorcontrib>OGAWA, Toru</creatorcontrib><creatorcontrib>AKAO, Kenichi</creatorcontrib><creatorcontrib>FUKUDA, Masaru</creatorcontrib><creatorcontrib>OGASAWARA, Toshiyuki</creatorcontrib><creatorcontrib>KITAGAWA, Yoshimasa</creatorcontrib><creatorcontrib>SANO, Kazuo</creatorcontrib><title>Observation of Molecular Changes of a Necrotic Tissue from a Murine Carcinoma by Fourier-Transform Infrared Microspectroscopy</title><title>Clinical cancer research</title><addtitle>Clin Cancer Res</addtitle><description>Purpose: Our purpose is to develop infrared (IR) microspectroscopy as a new optical diagnostic tool to support conventional lightscopic
techniques in investigating the viability of carcinoma tissues and to develop its use in the evaluation of the early effects
of anticancer therapy by monitoring the IR spectra in the necrotic area.
Experimental Design: We evaluated the tissue which amassed for 4 weeks after the isotransplantation of mouse squamous cell carcinoma into the
thigh of mice. The borders of the necrotic area of frozen tissue specimens were investigated by Fourier-transform IR microspectroscopy
and conventional histological staining.
Results: A significantly higher accumulation of cholesterol was observed in the necrotic tissue of a carcinoma. The mechanism of this
phenomenon is hitherto unrecognized. We proposed that the accumulated cholesterol may lie extracellularly as a result of the
ruptured plasma and internal membranes after the swelling of the necrotic cells brought on by hypoxia. The analysis of the
secondary structure of protein revealed that the amounts of β-sheet increased significantly in striking contrast to the decreasing
amounts of α-helix in a necrotic area of a carcinoma. It is plausible that this structural conversion of protein was because
of lipid-autooxidation products, such as cholesterol oxide but not cholesterol itself, which possesses cell toxicity and could
be generated in a necrotic area.
Conclusions: We conclude that it will be possible to evaluate the efficacy of the clinical treatment of carcinoma by monitoring subtle
biological changes of cholesterol absorbance in the early stage of necrosis because of anticancer treatment.</description><subject>Animals</subject><subject>Antineoplastic agents</subject><subject>Azo Compounds</subject><subject>Biological and medical sciences</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Chemotherapy</subject><subject>Cholesterol - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Necrosis</subject><subject>Pharmacology. Drug treatments</subject><subject>Protein Conformation</subject><subject>Soft Tissue Neoplasms - pathology</subject><subject>Spectroscopy, Fourier Transform Infrared - methods</subject><issn>1078-0432</issn><issn>1557-3265</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE9Lw0AQxYMotla_guxFPAUmf3Y3PUqwWmjtpZ7DZDNpVpJs2E2UHvzurrTi6T0ePx4z7yKYR5zLMIkFv_QeZBZCmsSz4Ma5D4AojSC9DmZRDAJEKufB9650ZD9x1KZnpmZb05KaWrQsb7A_kPsNkb2RsmbUiu21cxOx2prOx9vJ6p5Yjlbp3nTIyiNbGR-SDfcWe1cb27F1X1u0VLGt9i1uIDV6UWY43gZXNbaO7s66CN5Xz_v8NdzsXtb50yZsYrEcQ1kDoqwquRScCyUpLeusWlYlj7IU06wEKRSlooplqfy_FXCUnGMiABKBUbII7k-9w1R2VBWD1R3aY_G3gwcezgA6ha2_t1fa_XOJBOCQee7xxDX60HxpS4XyJFlLjvwITZEVooghguQHfA524Q</recordid><startdate>20020601</startdate><enddate>20020601</enddate><creator>YAMADA, Tetsushi</creator><creator>MIYOSHI, Norio</creator><creator>OGAWA, Toru</creator><creator>AKAO, Kenichi</creator><creator>FUKUDA, Masaru</creator><creator>OGASAWARA, Toshiyuki</creator><creator>KITAGAWA, Yoshimasa</creator><creator>SANO, Kazuo</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20020601</creationdate><title>Observation of Molecular Changes of a Necrotic Tissue from a Murine Carcinoma by Fourier-Transform Infrared Microspectroscopy</title><author>YAMADA, Tetsushi ; MIYOSHI, Norio ; OGAWA, Toru ; AKAO, Kenichi ; FUKUDA, Masaru ; OGASAWARA, Toshiyuki ; KITAGAWA, Yoshimasa ; SANO, Kazuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h269t-7f0aa7dd796556c7e4bf8d9db5184a48b076ce46d27bc043d05a755a360036a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Antineoplastic agents</topic><topic>Azo Compounds</topic><topic>Biological and medical sciences</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Chemotherapy</topic><topic>Cholesterol - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Necrosis</topic><topic>Pharmacology. Drug treatments</topic><topic>Protein Conformation</topic><topic>Soft Tissue Neoplasms - pathology</topic><topic>Spectroscopy, Fourier Transform Infrared - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YAMADA, Tetsushi</creatorcontrib><creatorcontrib>MIYOSHI, Norio</creatorcontrib><creatorcontrib>OGAWA, Toru</creatorcontrib><creatorcontrib>AKAO, Kenichi</creatorcontrib><creatorcontrib>FUKUDA, Masaru</creatorcontrib><creatorcontrib>OGASAWARA, Toshiyuki</creatorcontrib><creatorcontrib>KITAGAWA, Yoshimasa</creatorcontrib><creatorcontrib>SANO, Kazuo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Clinical cancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YAMADA, Tetsushi</au><au>MIYOSHI, Norio</au><au>OGAWA, Toru</au><au>AKAO, Kenichi</au><au>FUKUDA, Masaru</au><au>OGASAWARA, Toshiyuki</au><au>KITAGAWA, Yoshimasa</au><au>SANO, Kazuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Observation of Molecular Changes of a Necrotic Tissue from a Murine Carcinoma by Fourier-Transform Infrared Microspectroscopy</atitle><jtitle>Clinical cancer research</jtitle><addtitle>Clin Cancer Res</addtitle><date>2002-06-01</date><risdate>2002</risdate><volume>8</volume><issue>6</issue><spage>2010</spage><epage>2014</epage><pages>2010-2014</pages><issn>1078-0432</issn><eissn>1557-3265</eissn><abstract>Purpose: Our purpose is to develop infrared (IR) microspectroscopy as a new optical diagnostic tool to support conventional lightscopic
techniques in investigating the viability of carcinoma tissues and to develop its use in the evaluation of the early effects
of anticancer therapy by monitoring the IR spectra in the necrotic area.
Experimental Design: We evaluated the tissue which amassed for 4 weeks after the isotransplantation of mouse squamous cell carcinoma into the
thigh of mice. The borders of the necrotic area of frozen tissue specimens were investigated by Fourier-transform IR microspectroscopy
and conventional histological staining.
Results: A significantly higher accumulation of cholesterol was observed in the necrotic tissue of a carcinoma. The mechanism of this
phenomenon is hitherto unrecognized. We proposed that the accumulated cholesterol may lie extracellularly as a result of the
ruptured plasma and internal membranes after the swelling of the necrotic cells brought on by hypoxia. The analysis of the
secondary structure of protein revealed that the amounts of β-sheet increased significantly in striking contrast to the decreasing
amounts of α-helix in a necrotic area of a carcinoma. It is plausible that this structural conversion of protein was because
of lipid-autooxidation products, such as cholesterol oxide but not cholesterol itself, which possesses cell toxicity and could
be generated in a necrotic area.
Conclusions: We conclude that it will be possible to evaluate the efficacy of the clinical treatment of carcinoma by monitoring subtle
biological changes of cholesterol absorbance in the early stage of necrosis because of anticancer treatment.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>12060647</pmid><tpages>5</tpages></addata></record> |
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| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Antineoplastic agents Azo Compounds Biological and medical sciences Carcinoma, Squamous Cell - pathology Chemotherapy Cholesterol - metabolism Male Medical sciences Mice Mice, Inbred C3H Necrosis Pharmacology. Drug treatments Protein Conformation Soft Tissue Neoplasms - pathology Spectroscopy, Fourier Transform Infrared - methods |
| title | Observation of Molecular Changes of a Necrotic Tissue from a Murine Carcinoma by Fourier-Transform Infrared Microspectroscopy |
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