Endothelial nitric oxide production during in vitro simulation of external limb compression
1 Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge 02139; and 2 Vascular Surgery Research Laboratory, Division of Vascular Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114 External pneumatic compression (EPC) is eff...
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| Veröffentlicht in: | American journal of physiology. Heart and circulatory physiology 2002-06, Vol.282 (6), p.H2066-H2075 |
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| container_title | American journal of physiology. Heart and circulatory physiology |
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| creator | Dai, Guohao Tsukurov, Olga Chen, Michael Gertler, Jonathan P Kamm, Roger D |
| description | 1 Division of Biological Engineering, Massachusetts
Institute of Technology, Cambridge 02139; and
2 Vascular Surgery Research Laboratory, Division of
Vascular Surgery, Massachusetts General Hospital and Harvard
Medical School, Boston, Massachusetts 02114
External pneumatic
compression (EPC) is effective in preventing deep vein thrombosis (DVT)
and is thought to alter endothelial thromboresistant properties. We
investigated the effect of EPC on changes in nitric oxide (NO), a
critical mediator in the regulation of vasomotor and platelet function.
An in vitro cell culture system was developed to simulate flow and
vessel collapse conditions under EPC. Human umbilical vein endothelial
cells were cultured and subjected to tube compression (C), pulsatile
flow (F), or a combination of the two (FC). NO production and
endothelial nitric oxide synthase (eNOS) mRNA expression were measured.
The data demonstrate that in the F and FC groups, there is a rapid
release of NO followed by a sustained increase. NO production levels in the F and FC groups were almost identical, whereas the C group produced
the same low amount of NO as the control group. Conditions F and FC
also upregulate eNOS mRNA expression by a factor of 2.08 ± 0.25 and 2.11 ± 0.21, respectively, at 6 h. Experiments with different modes of EPC show that NO production and eNOS mRNA expression respond to different time cycles of compression. These results implicate enhanced NO release as a potentially important factor in the
prevention of DVT.
deep vein thrombosis; hemodynamics; nitric oxide synthase |
| doi_str_mv | 10.1152/ajpheart.00288.2001 |
| format | Article |
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Institute of Technology, Cambridge 02139; and
2 Vascular Surgery Research Laboratory, Division of
Vascular Surgery, Massachusetts General Hospital and Harvard
Medical School, Boston, Massachusetts 02114
External pneumatic
compression (EPC) is effective in preventing deep vein thrombosis (DVT)
and is thought to alter endothelial thromboresistant properties. We
investigated the effect of EPC on changes in nitric oxide (NO), a
critical mediator in the regulation of vasomotor and platelet function.
An in vitro cell culture system was developed to simulate flow and
vessel collapse conditions under EPC. Human umbilical vein endothelial
cells were cultured and subjected to tube compression (C), pulsatile
flow (F), or a combination of the two (FC). NO production and
endothelial nitric oxide synthase (eNOS) mRNA expression were measured.
The data demonstrate that in the F and FC groups, there is a rapid
release of NO followed by a sustained increase. NO production levels in the F and FC groups were almost identical, whereas the C group produced
the same low amount of NO as the control group. Conditions F and FC
also upregulate eNOS mRNA expression by a factor of 2.08 ± 0.25 and 2.11 ± 0.21, respectively, at 6 h. Experiments with different modes of EPC show that NO production and eNOS mRNA expression respond to different time cycles of compression. These results implicate enhanced NO release as a potentially important factor in the
prevention of DVT.
deep vein thrombosis; hemodynamics; nitric oxide synthase</description><identifier>ISSN: 0363-6135</identifier><identifier>EISSN: 1522-1539</identifier><identifier>DOI: 10.1152/ajpheart.00288.2001</identifier><identifier>PMID: 12003813</identifier><language>eng</language><publisher>United States</publisher><subject>Blotting, Northern ; Cells, Cultured ; Culture Media, Conditioned ; Endothelium, Vascular - metabolism ; Enzyme Inhibitors - pharmacology ; Gravity Suits ; Humans ; Models, Biological ; Nitric Oxide - biosynthesis ; Nitric Oxide Synthase - antagonists & inhibitors ; Nitric Oxide Synthase - genetics ; Nitric Oxide Synthase Type I ; Nitric Oxide Synthase Type II ; Nitric Oxide Synthase Type III ; Nitrites - metabolism ; Pressure ; Pulsatile Flow ; Rheology ; RNA, Messenger - analysis ; Space life sciences ; Umbilical Veins ; Venous Thrombosis - prevention & control</subject><ispartof>American journal of physiology. Heart and circulatory physiology, 2002-06, Vol.282 (6), p.H2066-H2075</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-bfc02c246ae36fe6dd0a356d398e8b34a36180f178456ce9036d40404e055ad73</citedby><cites>FETCH-LOGICAL-c459t-bfc02c246ae36fe6dd0a356d398e8b34a36180f178456ce9036d40404e055ad73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,3040,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12003813$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dai, Guohao</creatorcontrib><creatorcontrib>Tsukurov, Olga</creatorcontrib><creatorcontrib>Chen, Michael</creatorcontrib><creatorcontrib>Gertler, Jonathan P</creatorcontrib><creatorcontrib>Kamm, Roger D</creatorcontrib><title>Endothelial nitric oxide production during in vitro simulation of external limb compression</title><title>American journal of physiology. Heart and circulatory physiology</title><addtitle>Am J Physiol Heart Circ Physiol</addtitle><description>1 Division of Biological Engineering, Massachusetts
Institute of Technology, Cambridge 02139; and
2 Vascular Surgery Research Laboratory, Division of
Vascular Surgery, Massachusetts General Hospital and Harvard
Medical School, Boston, Massachusetts 02114
External pneumatic
compression (EPC) is effective in preventing deep vein thrombosis (DVT)
and is thought to alter endothelial thromboresistant properties. We
investigated the effect of EPC on changes in nitric oxide (NO), a
critical mediator in the regulation of vasomotor and platelet function.
An in vitro cell culture system was developed to simulate flow and
vessel collapse conditions under EPC. Human umbilical vein endothelial
cells were cultured and subjected to tube compression (C), pulsatile
flow (F), or a combination of the two (FC). NO production and
endothelial nitric oxide synthase (eNOS) mRNA expression were measured.
The data demonstrate that in the F and FC groups, there is a rapid
release of NO followed by a sustained increase. NO production levels in the F and FC groups were almost identical, whereas the C group produced
the same low amount of NO as the control group. Conditions F and FC
also upregulate eNOS mRNA expression by a factor of 2.08 ± 0.25 and 2.11 ± 0.21, respectively, at 6 h. Experiments with different modes of EPC show that NO production and eNOS mRNA expression respond to different time cycles of compression. These results implicate enhanced NO release as a potentially important factor in the
prevention of DVT.
deep vein thrombosis; hemodynamics; nitric oxide synthase</description><subject>Blotting, Northern</subject><subject>Cells, Cultured</subject><subject>Culture Media, Conditioned</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Gravity Suits</subject><subject>Humans</subject><subject>Models, Biological</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitric Oxide Synthase - antagonists & inhibitors</subject><subject>Nitric Oxide Synthase - genetics</subject><subject>Nitric Oxide Synthase Type I</subject><subject>Nitric Oxide Synthase Type II</subject><subject>Nitric Oxide Synthase Type III</subject><subject>Nitrites - metabolism</subject><subject>Pressure</subject><subject>Pulsatile Flow</subject><subject>Rheology</subject><subject>RNA, Messenger - analysis</subject><subject>Space life sciences</subject><subject>Umbilical Veins</subject><subject>Venous Thrombosis - prevention & control</subject><issn>0363-6135</issn><issn>1522-1539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEFvFCEYhonR2LX6C0wMJ2-z_YCBmY0n07TWpImXevJAWPhmh4YZRpix3X8v212tF8OBw_s-b-Ah5D2DNWOSX5j7qUeT5jUAb9s1B2AvyKokvGJSbF6SFQglKsWEPCNvcr4HANko8ZqcsVIWLRMr8uNqdHHuMXgT6Ojn5C2Nj94hnVJ0i519HKlbkh931I_0V2lEmv2wBPMUxY7i44xpLHjww5baOEwJcy7hW_KqMyHju9N9Tr5fX91d3lS33758vfx8W9labuZq21ngltfKoFAdKufACKmc2LTYbkVthGItdKxpa6ksbsqvXA3lIEhpXCPOycfjbnnyzwXzrAefLYZgRoxL1g1rQDTqUBTHok0x54SdnpIfTNprBvrgVP9xqp-c6oPTQn04zS_bAd0zc5JYChfHQu93_YNPqKd-XwSEuNs_L_KWa6VvOChViE__J66XEO6K1L_oP6SeXCd-A-YQnFo</recordid><startdate>20020601</startdate><enddate>20020601</enddate><creator>Dai, Guohao</creator><creator>Tsukurov, Olga</creator><creator>Chen, Michael</creator><creator>Gertler, Jonathan P</creator><creator>Kamm, Roger D</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020601</creationdate><title>Endothelial nitric oxide production during in vitro simulation of external limb compression</title><author>Dai, Guohao ; Tsukurov, Olga ; Chen, Michael ; Gertler, Jonathan P ; Kamm, Roger D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-bfc02c246ae36fe6dd0a356d398e8b34a36180f178456ce9036d40404e055ad73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Blotting, Northern</topic><topic>Cells, Cultured</topic><topic>Culture Media, Conditioned</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Gravity Suits</topic><topic>Humans</topic><topic>Models, Biological</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitric Oxide Synthase - antagonists & inhibitors</topic><topic>Nitric Oxide Synthase - genetics</topic><topic>Nitric Oxide Synthase Type I</topic><topic>Nitric Oxide Synthase Type II</topic><topic>Nitric Oxide Synthase Type III</topic><topic>Nitrites - metabolism</topic><topic>Pressure</topic><topic>Pulsatile Flow</topic><topic>Rheology</topic><topic>RNA, Messenger - analysis</topic><topic>Space life sciences</topic><topic>Umbilical Veins</topic><topic>Venous Thrombosis - prevention & control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dai, Guohao</creatorcontrib><creatorcontrib>Tsukurov, Olga</creatorcontrib><creatorcontrib>Chen, Michael</creatorcontrib><creatorcontrib>Gertler, Jonathan P</creatorcontrib><creatorcontrib>Kamm, Roger D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. Heart and circulatory physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dai, Guohao</au><au>Tsukurov, Olga</au><au>Chen, Michael</au><au>Gertler, Jonathan P</au><au>Kamm, Roger D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endothelial nitric oxide production during in vitro simulation of external limb compression</atitle><jtitle>American journal of physiology. Heart and circulatory physiology</jtitle><addtitle>Am J Physiol Heart Circ Physiol</addtitle><date>2002-06-01</date><risdate>2002</risdate><volume>282</volume><issue>6</issue><spage>H2066</spage><epage>H2075</epage><pages>H2066-H2075</pages><issn>0363-6135</issn><eissn>1522-1539</eissn><abstract>1 Division of Biological Engineering, Massachusetts
Institute of Technology, Cambridge 02139; and
2 Vascular Surgery Research Laboratory, Division of
Vascular Surgery, Massachusetts General Hospital and Harvard
Medical School, Boston, Massachusetts 02114
External pneumatic
compression (EPC) is effective in preventing deep vein thrombosis (DVT)
and is thought to alter endothelial thromboresistant properties. We
investigated the effect of EPC on changes in nitric oxide (NO), a
critical mediator in the regulation of vasomotor and platelet function.
An in vitro cell culture system was developed to simulate flow and
vessel collapse conditions under EPC. Human umbilical vein endothelial
cells were cultured and subjected to tube compression (C), pulsatile
flow (F), or a combination of the two (FC). NO production and
endothelial nitric oxide synthase (eNOS) mRNA expression were measured.
The data demonstrate that in the F and FC groups, there is a rapid
release of NO followed by a sustained increase. NO production levels in the F and FC groups were almost identical, whereas the C group produced
the same low amount of NO as the control group. Conditions F and FC
also upregulate eNOS mRNA expression by a factor of 2.08 ± 0.25 and 2.11 ± 0.21, respectively, at 6 h. Experiments with different modes of EPC show that NO production and eNOS mRNA expression respond to different time cycles of compression. These results implicate enhanced NO release as a potentially important factor in the
prevention of DVT.
deep vein thrombosis; hemodynamics; nitric oxide synthase</abstract><cop>United States</cop><pmid>12003813</pmid><doi>10.1152/ajpheart.00288.2001</doi></addata></record> |
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| issn | 0363-6135 1522-1539 |
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| recordid | cdi_pubmed_primary_12003813 |
| source | MEDLINE; American Physiological Society; EZB-FREE-00999 freely available EZB journals |
| subjects | Blotting, Northern Cells, Cultured Culture Media, Conditioned Endothelium, Vascular - metabolism Enzyme Inhibitors - pharmacology Gravity Suits Humans Models, Biological Nitric Oxide - biosynthesis Nitric Oxide Synthase - antagonists & inhibitors Nitric Oxide Synthase - genetics Nitric Oxide Synthase Type I Nitric Oxide Synthase Type II Nitric Oxide Synthase Type III Nitrites - metabolism Pressure Pulsatile Flow Rheology RNA, Messenger - analysis Space life sciences Umbilical Veins Venous Thrombosis - prevention & control |
| title | Endothelial nitric oxide production during in vitro simulation of external limb compression |
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