Pollen-mediated introgression and hybrid speciation in Louisiana irises
Populations of the "Louisiana iris" species Iris fulva, I. hexagona, and I. nelsonii were examined genetically to test for interspecific gene flow between I. fulva and I. hexagona, for pollen- versus seed-mediated introgression between these species, and for the presumed hybrid origin of I...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1991-02, Vol.88 (4), p.1398-1402 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Arnold, M.L. (University of Georgia, Athens, GA) Buckner, C.M Robinson, J.J |
| description | Populations of the "Louisiana iris" species Iris fulva, I. hexagona, and I. nelsonii were examined genetically to test for interspecific gene flow between I. fulva and I. hexagona, for pollen- versus seed-mediated introgression between these species, and for the presumed hybrid origin of I. nelsonii. Genetic markers were identified by using both a polymerase chain reaction-like method that allows the identification of random, nuclear markers and standard polymerase chain reaction experiments involving specific chloroplast DNA (cpDNA) oligonucleotides. Restriction endonuclease digestions of the cpDNA amplification products resolved diagnostic restriction site differences for I. fulva and I. hexagona. The distribution of the species-specific nuclear markers supports a hypothesis of bidirectional introgression between I. fulva and I. hexagona. Thus, individuals analyzed from a contemporary hybrid population demonstrate multilocus genotypes that are indicative of advanced-generation hybrid individuals. Furthermore, several markers from the alternate species were present in low frequency in one allopatric population each of I. fulva and I. hexagona. Data from the nuclear analysis also support the hypothesized hybrid origin of I. nelsonii from the interaction of I. fulva and I. hexagona. Finally, cpDNA data support the hypothesis that the localized and the dispersed introgression are largely due to pollen transfer. In addition to the biological implications, this study demonstrates the power of the polymerase chain reaction methodology for the rapid identification of random and specific genetic markers for testing evolutionary genetic hypotheses. |
| doi_str_mv | 10.1073/pnas.88.4.1398 |
| format | Article |
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(University of Georgia, Athens, GA) ; Buckner, C.M ; Robinson, J.J</creator><creatorcontrib>Arnold, M.L. (University of Georgia, Athens, GA) ; Buckner, C.M ; Robinson, J.J</creatorcontrib><description>Populations of the "Louisiana iris" species Iris fulva, I. hexagona, and I. nelsonii were examined genetically to test for interspecific gene flow between I. fulva and I. hexagona, for pollen- versus seed-mediated introgression between these species, and for the presumed hybrid origin of I. nelsonii. Genetic markers were identified by using both a polymerase chain reaction-like method that allows the identification of random, nuclear markers and standard polymerase chain reaction experiments involving specific chloroplast DNA (cpDNA) oligonucleotides. Restriction endonuclease digestions of the cpDNA amplification products resolved diagnostic restriction site differences for I. fulva and I. hexagona. The distribution of the species-specific nuclear markers supports a hypothesis of bidirectional introgression between I. fulva and I. hexagona. Thus, individuals analyzed from a contemporary hybrid population demonstrate multilocus genotypes that are indicative of advanced-generation hybrid individuals. Furthermore, several markers from the alternate species were present in low frequency in one allopatric population each of I. fulva and I. hexagona. Data from the nuclear analysis also support the hypothesized hybrid origin of I. nelsonii from the interaction of I. fulva and I. hexagona. Finally, cpDNA data support the hypothesis that the localized and the dispersed introgression are largely due to pollen transfer. In addition to the biological implications, this study demonstrates the power of the polymerase chain reaction methodology for the rapid identification of random and specific genetic markers for testing evolutionary genetic hypotheses.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.88.4.1398</identifier><identifier>PMID: 11607153</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Bayous ; BIOCHEMISTRY ; BIOCHIMIE ; Biological and medical sciences ; BIOQUIMICA ; Classical genetics, quantitative genetics, hybrids ; DNA ; Evolution ; Fundamental and applied biological sciences. Psychology ; GENE ; GENE FLOW ; GENES ; Genetic hybridization ; GENETIC MARKERS ; Genetics of eukaryotes. Biological and molecular evolution ; Genomics ; Haplotypes ; HIBRIDACION INTERESPECIFICA ; HYBRIDATION INTERSPECIFIQUE ; Hybridity ; INTERSPECIFIC HYBRIDIZATION ; INTROGRESION ; INTROGRESSION ; IRIS ; LOUISIANA ; LOUISIANE ; LUISIANA ; MARCADORES GENETICOS ; MARQUEUR GENETIQUE ; POLIMORFISMO ; Pollen ; POLYMERASE CHAIN REACTION ; POLYMORPHISM ; POLYMORPHISME ; Pteridophyta, spermatophyta ; RESTRICTION FRAGMENT LENGTH POLYMORPHISM ; Vegetals</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1991-02, Vol.88 (4), p.1398-1402</ispartof><rights>Copyright 1991 The National Academy of Sciences of the United States of America</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c573t-60248ad6862246a5de340e4460640d099d5e93d5a35673796c7f76b3383b6b993</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/88/4.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2356002$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2356002$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19828878$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11607153$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arnold, M.L. (University of Georgia, Athens, GA)</creatorcontrib><creatorcontrib>Buckner, C.M</creatorcontrib><creatorcontrib>Robinson, J.J</creatorcontrib><title>Pollen-mediated introgression and hybrid speciation in Louisiana irises</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Populations of the "Louisiana iris" species Iris fulva, I. hexagona, and I. nelsonii were examined genetically to test for interspecific gene flow between I. fulva and I. hexagona, for pollen- versus seed-mediated introgression between these species, and for the presumed hybrid origin of I. nelsonii. Genetic markers were identified by using both a polymerase chain reaction-like method that allows the identification of random, nuclear markers and standard polymerase chain reaction experiments involving specific chloroplast DNA (cpDNA) oligonucleotides. Restriction endonuclease digestions of the cpDNA amplification products resolved diagnostic restriction site differences for I. fulva and I. hexagona. The distribution of the species-specific nuclear markers supports a hypothesis of bidirectional introgression between I. fulva and I. hexagona. Thus, individuals analyzed from a contemporary hybrid population demonstrate multilocus genotypes that are indicative of advanced-generation hybrid individuals. Furthermore, several markers from the alternate species were present in low frequency in one allopatric population each of I. fulva and I. hexagona. Data from the nuclear analysis also support the hypothesized hybrid origin of I. nelsonii from the interaction of I. fulva and I. hexagona. Finally, cpDNA data support the hypothesis that the localized and the dispersed introgression are largely due to pollen transfer. In addition to the biological implications, this study demonstrates the power of the polymerase chain reaction methodology for the rapid identification of random and specific genetic markers for testing evolutionary genetic hypotheses.</description><subject>Bayous</subject><subject>BIOCHEMISTRY</subject><subject>BIOCHIMIE</subject><subject>Biological and medical sciences</subject><subject>BIOQUIMICA</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>DNA</subject><subject>Evolution</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENE FLOW</subject><subject>GENES</subject><subject>Genetic hybridization</subject><subject>GENETIC MARKERS</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Genomics</subject><subject>Haplotypes</subject><subject>HIBRIDACION INTERESPECIFICA</subject><subject>HYBRIDATION INTERSPECIFIQUE</subject><subject>Hybridity</subject><subject>INTERSPECIFIC HYBRIDIZATION</subject><subject>INTROGRESION</subject><subject>INTROGRESSION</subject><subject>IRIS</subject><subject>LOUISIANA</subject><subject>LOUISIANE</subject><subject>LUISIANA</subject><subject>MARCADORES GENETICOS</subject><subject>MARQUEUR GENETIQUE</subject><subject>POLIMORFISMO</subject><subject>Pollen</subject><subject>POLYMERASE CHAIN REACTION</subject><subject>POLYMORPHISM</subject><subject>POLYMORPHISME</subject><subject>Pteridophyta, spermatophyta</subject><subject>RESTRICTION FRAGMENT LENGTH POLYMORPHISM</subject><subject>Vegetals</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNp9ks1v1DAQxS0EokvhygEByoXeEsafsaVeUAUFaSWQoGfLiZ2tq6y92Ami_z2ONrTlwsmS32_mzTwboZcYGgwtfX8IJjdSNqzBVMlHaINB4VowBY_RBoC0tWSEnaBnOd8AgOISnqITjAW0mNMNuvwWx9GFeu-sN5OzlQ9TirvkcvYxVCbY6vq2S95W-eD6giy3PlTbOPvsTTCVTz67_Bw9GcyY3Yv1PEVXnz7-uPhcb79efrn4sK173tKpFkCYNFZIQQgThltHGTjGBAgGFpSy3ClquaFctLRVom-HVnSUStqJTil6is6PfQ9zV2buXRnXjPqQ_N6kWx2N1_8qwV_rXfylOQbCS_nZWp7iz9nlSe997t04muDinLUEyinDi09zBPsUc05uuLPAoJfk9ZK8llIzvSRfCt4-HOweX6MuwLsVMLk345BM6H2-55QkUrZLozcrtxj8lR8anf1P18M8jpP7PRXw9RG8yVNMdyQp0ZaPUeRXR3kwUZtdeUV99V1hQsr-9A83qrXO</recordid><startdate>19910215</startdate><enddate>19910215</enddate><creator>Arnold, M.L. 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(University of Georgia, Athens, GA) ; Buckner, C.M ; Robinson, J.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c573t-60248ad6862246a5de340e4460640d099d5e93d5a35673796c7f76b3383b6b993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Bayous</topic><topic>BIOCHEMISTRY</topic><topic>BIOCHIMIE</topic><topic>Biological and medical sciences</topic><topic>BIOQUIMICA</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>DNA</topic><topic>Evolution</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENE FLOW</topic><topic>GENES</topic><topic>Genetic hybridization</topic><topic>GENETIC MARKERS</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Genomics</topic><topic>Haplotypes</topic><topic>HIBRIDACION INTERESPECIFICA</topic><topic>HYBRIDATION INTERSPECIFIQUE</topic><topic>Hybridity</topic><topic>INTERSPECIFIC HYBRIDIZATION</topic><topic>INTROGRESION</topic><topic>INTROGRESSION</topic><topic>IRIS</topic><topic>LOUISIANA</topic><topic>LOUISIANE</topic><topic>LUISIANA</topic><topic>MARCADORES GENETICOS</topic><topic>MARQUEUR GENETIQUE</topic><topic>POLIMORFISMO</topic><topic>Pollen</topic><topic>POLYMERASE CHAIN REACTION</topic><topic>POLYMORPHISM</topic><topic>POLYMORPHISME</topic><topic>Pteridophyta, spermatophyta</topic><topic>RESTRICTION FRAGMENT LENGTH POLYMORPHISM</topic><topic>Vegetals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arnold, M.L. (University of Georgia, Athens, GA)</creatorcontrib><creatorcontrib>Buckner, C.M</creatorcontrib><creatorcontrib>Robinson, J.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arnold, M.L. (University of Georgia, Athens, GA)</au><au>Buckner, C.M</au><au>Robinson, J.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pollen-mediated introgression and hybrid speciation in Louisiana irises</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1991-02-15</date><risdate>1991</risdate><volume>88</volume><issue>4</issue><spage>1398</spage><epage>1402</epage><pages>1398-1402</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Populations of the "Louisiana iris" species Iris fulva, I. hexagona, and I. nelsonii were examined genetically to test for interspecific gene flow between I. fulva and I. hexagona, for pollen- versus seed-mediated introgression between these species, and for the presumed hybrid origin of I. nelsonii. Genetic markers were identified by using both a polymerase chain reaction-like method that allows the identification of random, nuclear markers and standard polymerase chain reaction experiments involving specific chloroplast DNA (cpDNA) oligonucleotides. Restriction endonuclease digestions of the cpDNA amplification products resolved diagnostic restriction site differences for I. fulva and I. hexagona. The distribution of the species-specific nuclear markers supports a hypothesis of bidirectional introgression between I. fulva and I. hexagona. Thus, individuals analyzed from a contemporary hybrid population demonstrate multilocus genotypes that are indicative of advanced-generation hybrid individuals. Furthermore, several markers from the alternate species were present in low frequency in one allopatric population each of I. fulva and I. hexagona. Data from the nuclear analysis also support the hypothesized hybrid origin of I. nelsonii from the interaction of I. fulva and I. hexagona. Finally, cpDNA data support the hypothesis that the localized and the dispersed introgression are largely due to pollen transfer. In addition to the biological implications, this study demonstrates the power of the polymerase chain reaction methodology for the rapid identification of random and specific genetic markers for testing evolutionary genetic hypotheses.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>11607153</pmid><doi>10.1073/pnas.88.4.1398</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1991-02, Vol.88 (4), p.1398-1402 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Bayous BIOCHEMISTRY BIOCHIMIE Biological and medical sciences BIOQUIMICA Classical genetics, quantitative genetics, hybrids DNA Evolution Fundamental and applied biological sciences. Psychology GENE GENE FLOW GENES Genetic hybridization GENETIC MARKERS Genetics of eukaryotes. Biological and molecular evolution Genomics Haplotypes HIBRIDACION INTERESPECIFICA HYBRIDATION INTERSPECIFIQUE Hybridity INTERSPECIFIC HYBRIDIZATION INTROGRESION INTROGRESSION IRIS LOUISIANA LOUISIANE LUISIANA MARCADORES GENETICOS MARQUEUR GENETIQUE POLIMORFISMO Pollen POLYMERASE CHAIN REACTION POLYMORPHISM POLYMORPHISME Pteridophyta, spermatophyta RESTRICTION FRAGMENT LENGTH POLYMORPHISM Vegetals |
| title | Pollen-mediated introgression and hybrid speciation in Louisiana irises |
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