Role of leukocytes in uterine hypoperfusion and fetal growth retardation induced by ischemia-reperfusion
1 Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo 160-8582; and 2 Department of Clinical Research, National Ohkura Hospital, Tokyo 157-0063, Japan We investigated leukocyte involvement in uterine hypoperfusion and intrauterine fetal growth retardation (IUGR) indu...
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| Veröffentlicht in: | American journal of physiology. Heart and circulatory physiology 2001-03, Vol.280 (3), p.H1215-H1221 |
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| container_title | American journal of physiology. Heart and circulatory physiology |
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| creator | Miyakoshi, Kei Ishimoto, Hitoshi Nishimura, Osamu Tanigaki, Shinji Tanaka, Mamoru Miyazaki, Toyohiko Natori, Michiya Yoshimura, Yasunori |
| description | 1 Department of Obstetrics and Gynecology, Keio University
School of Medicine, Tokyo 160-8582; and 2 Department of
Clinical Research, National Ohkura Hospital, Tokyo 157-0063, Japan
We
investigated leukocyte involvement in uterine hypoperfusion and
intrauterine fetal growth retardation (IUGR) induced by ischemia-reperfusion (I/R) in Sprague-Dawley rats. On day
17 of gestation, leukocyte accumulation in the uterus and placenta
subjected to 30 min of ischemia, followed by reperfusion, was
assessed by measuring myeloperoxidase (MPO) activity. Uterine MPO
activity was significantly higher after 1 h of reperfusion than it
was before ischemia ( P |
| doi_str_mv | 10.1152/ajpheart.2001.280.3.h1215 |
| format | Article |
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School of Medicine, Tokyo 160-8582; and 2 Department of
Clinical Research, National Ohkura Hospital, Tokyo 157-0063, Japan
We
investigated leukocyte involvement in uterine hypoperfusion and
intrauterine fetal growth retardation (IUGR) induced by ischemia-reperfusion (I/R) in Sprague-Dawley rats. On day
17 of gestation, leukocyte accumulation in the uterus and placenta
subjected to 30 min of ischemia, followed by reperfusion, was
assessed by measuring myeloperoxidase (MPO) activity. Uterine MPO
activity was significantly higher after 1 h of reperfusion than it
was before ischemia ( P < 0.05), without any
increase in placental MPO activity. Immunohistochemical staining showed
leukocyte accumulation in the uterus subjected to I/R. The effects of
treatment with monoclonal antibodies against CD11a (WT1) and CD18 (WT3)
at a dose of 0.8 mg/kg on uterine blood flow and IUGR were
investigated. Laser-Doppler flowmetry demonstrated that uterine
hypoperfusion at 2 h after ischemia (blood flow,
51.7 ± 1.2%; P < 0.01) was inhibited by WT1 and
WT3 treatment. I/R-induced IUGR at full term ( P < 0.05 vs. nonischemic horn) was prevented by WT1 and WT3 treatment on
day 17 . These results indicate that leukocyte accumulation may play an important role in the pathogenesis of uterine hypoperfusion and IUGR induced by I/R in pregnant rats.
uterine blood flow; CD11/CD18 integrin; rat</description><identifier>ISSN: 0363-6135</identifier><identifier>EISSN: 1522-1539</identifier><identifier>DOI: 10.1152/ajpheart.2001.280.3.h1215</identifier><identifier>PMID: 11179066</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Antibodies, Monoclonal - pharmacology ; CD18 Antigens - immunology ; Female ; Fetal Growth Retardation - immunology ; Fetal Growth Retardation - pathology ; Fetal Growth Retardation - physiopathology ; Fetal Weight ; Leukocytes - physiology ; Lymphocyte Function-Associated Antigen-1 - immunology ; Organ Size ; Placenta - blood supply ; Placenta - immunology ; Placenta - pathology ; Pregnancy ; Rats ; Reperfusion Injury - immunology ; Reperfusion Injury - pathology ; Reperfusion Injury - physiopathology ; Uterus - blood supply ; Uterus - immunology ; Uterus - physiopathology</subject><ispartof>American journal of physiology. Heart and circulatory physiology, 2001-03, Vol.280 (3), p.H1215-H1221</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-f16bf7d36bffd1aebaf9284dd948f56092a51b36ea72f8b72c319f33c39178ef3</citedby><cites>FETCH-LOGICAL-c471t-f16bf7d36bffd1aebaf9284dd948f56092a51b36ea72f8b72c319f33c39178ef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3026,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11179066$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miyakoshi, Kei</creatorcontrib><creatorcontrib>Ishimoto, Hitoshi</creatorcontrib><creatorcontrib>Nishimura, Osamu</creatorcontrib><creatorcontrib>Tanigaki, Shinji</creatorcontrib><creatorcontrib>Tanaka, Mamoru</creatorcontrib><creatorcontrib>Miyazaki, Toyohiko</creatorcontrib><creatorcontrib>Natori, Michiya</creatorcontrib><creatorcontrib>Yoshimura, Yasunori</creatorcontrib><title>Role of leukocytes in uterine hypoperfusion and fetal growth retardation induced by ischemia-reperfusion</title><title>American journal of physiology. Heart and circulatory physiology</title><addtitle>Am J Physiol Heart Circ Physiol</addtitle><description>1 Department of Obstetrics and Gynecology, Keio University
School of Medicine, Tokyo 160-8582; and 2 Department of
Clinical Research, National Ohkura Hospital, Tokyo 157-0063, Japan
We
investigated leukocyte involvement in uterine hypoperfusion and
intrauterine fetal growth retardation (IUGR) induced by ischemia-reperfusion (I/R) in Sprague-Dawley rats. On day
17 of gestation, leukocyte accumulation in the uterus and placenta
subjected to 30 min of ischemia, followed by reperfusion, was
assessed by measuring myeloperoxidase (MPO) activity. Uterine MPO
activity was significantly higher after 1 h of reperfusion than it
was before ischemia ( P < 0.05), without any
increase in placental MPO activity. Immunohistochemical staining showed
leukocyte accumulation in the uterus subjected to I/R. The effects of
treatment with monoclonal antibodies against CD11a (WT1) and CD18 (WT3)
at a dose of 0.8 mg/kg on uterine blood flow and IUGR were
investigated. Laser-Doppler flowmetry demonstrated that uterine
hypoperfusion at 2 h after ischemia (blood flow,
51.7 ± 1.2%; P < 0.01) was inhibited by WT1 and
WT3 treatment. I/R-induced IUGR at full term ( P < 0.05 vs. nonischemic horn) was prevented by WT1 and WT3 treatment on
day 17 . These results indicate that leukocyte accumulation may play an important role in the pathogenesis of uterine hypoperfusion and IUGR induced by I/R in pregnant rats.
uterine blood flow; CD11/CD18 integrin; rat</description><subject>Animals</subject><subject>Antibodies, Monoclonal - pharmacology</subject><subject>CD18 Antigens - immunology</subject><subject>Female</subject><subject>Fetal Growth Retardation - immunology</subject><subject>Fetal Growth Retardation - pathology</subject><subject>Fetal Growth Retardation - physiopathology</subject><subject>Fetal Weight</subject><subject>Leukocytes - physiology</subject><subject>Lymphocyte Function-Associated Antigen-1 - immunology</subject><subject>Organ Size</subject><subject>Placenta - blood supply</subject><subject>Placenta - immunology</subject><subject>Placenta - pathology</subject><subject>Pregnancy</subject><subject>Rats</subject><subject>Reperfusion Injury - immunology</subject><subject>Reperfusion Injury - pathology</subject><subject>Reperfusion Injury - physiopathology</subject><subject>Uterus - blood supply</subject><subject>Uterus - immunology</subject><subject>Uterus - physiopathology</subject><issn>0363-6135</issn><issn>1522-1539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1r3DAQhkVpaTYff6Gol97saKy1ZNFTCU1SCBRKehayNVor9VquZJP638dmNx-XXkYM874zrx5CPgPLAcri0jwMLZo45gVjkBcVy3neQgHlO7JZ5kUGJVfvyYZxwTMBvDwhpyk9MMZKKfhHcgIAUjEhNqT9FTqkwdEOpz-hmUdM1Pd0GjH6Hmk7D2HA6KbkQ09Nb6nD0XR0F8Pj2NK4NNGacR363k4NWlrP1Kemxb03WcQX8zn54EyX8OL4npHf19_vr26zu583P66-3WXNVsKYORC1k5Yv1VkwWBunimprrdpWrhRMFaaEmgs0snBVLYuGg3KcN1yBrNDxM_LlsHeI4e-EadT7JQ52nekxTElLJrZScbUI1UHYxJBSRKeH6PcmzhqYXjHrZ8x6xawXzJrr2xXz4v10PDLVe7SvziPXRfD1IGj9rn30EfXQzguGLuxmfT113T3-G18OvFmtB7v-4fL_7tdUbwI9AQBNpU0</recordid><startdate>20010301</startdate><enddate>20010301</enddate><creator>Miyakoshi, Kei</creator><creator>Ishimoto, Hitoshi</creator><creator>Nishimura, Osamu</creator><creator>Tanigaki, Shinji</creator><creator>Tanaka, Mamoru</creator><creator>Miyazaki, Toyohiko</creator><creator>Natori, Michiya</creator><creator>Yoshimura, Yasunori</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010301</creationdate><title>Role of leukocytes in uterine hypoperfusion and fetal growth retardation induced by ischemia-reperfusion</title><author>Miyakoshi, Kei ; Ishimoto, Hitoshi ; Nishimura, Osamu ; Tanigaki, Shinji ; Tanaka, Mamoru ; Miyazaki, Toyohiko ; Natori, Michiya ; Yoshimura, Yasunori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-f16bf7d36bffd1aebaf9284dd948f56092a51b36ea72f8b72c319f33c39178ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - pharmacology</topic><topic>CD18 Antigens - immunology</topic><topic>Female</topic><topic>Fetal Growth Retardation - immunology</topic><topic>Fetal Growth Retardation - pathology</topic><topic>Fetal Growth Retardation - physiopathology</topic><topic>Fetal Weight</topic><topic>Leukocytes - physiology</topic><topic>Lymphocyte Function-Associated Antigen-1 - immunology</topic><topic>Organ Size</topic><topic>Placenta - blood supply</topic><topic>Placenta - immunology</topic><topic>Placenta - pathology</topic><topic>Pregnancy</topic><topic>Rats</topic><topic>Reperfusion Injury - immunology</topic><topic>Reperfusion Injury - pathology</topic><topic>Reperfusion Injury - physiopathology</topic><topic>Uterus - blood supply</topic><topic>Uterus - immunology</topic><topic>Uterus - physiopathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miyakoshi, Kei</creatorcontrib><creatorcontrib>Ishimoto, Hitoshi</creatorcontrib><creatorcontrib>Nishimura, Osamu</creatorcontrib><creatorcontrib>Tanigaki, Shinji</creatorcontrib><creatorcontrib>Tanaka, Mamoru</creatorcontrib><creatorcontrib>Miyazaki, Toyohiko</creatorcontrib><creatorcontrib>Natori, Michiya</creatorcontrib><creatorcontrib>Yoshimura, Yasunori</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. Heart and circulatory physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miyakoshi, Kei</au><au>Ishimoto, Hitoshi</au><au>Nishimura, Osamu</au><au>Tanigaki, Shinji</au><au>Tanaka, Mamoru</au><au>Miyazaki, Toyohiko</au><au>Natori, Michiya</au><au>Yoshimura, Yasunori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of leukocytes in uterine hypoperfusion and fetal growth retardation induced by ischemia-reperfusion</atitle><jtitle>American journal of physiology. Heart and circulatory physiology</jtitle><addtitle>Am J Physiol Heart Circ Physiol</addtitle><date>2001-03-01</date><risdate>2001</risdate><volume>280</volume><issue>3</issue><spage>H1215</spage><epage>H1221</epage><pages>H1215-H1221</pages><issn>0363-6135</issn><eissn>1522-1539</eissn><abstract>1 Department of Obstetrics and Gynecology, Keio University
School of Medicine, Tokyo 160-8582; and 2 Department of
Clinical Research, National Ohkura Hospital, Tokyo 157-0063, Japan
We
investigated leukocyte involvement in uterine hypoperfusion and
intrauterine fetal growth retardation (IUGR) induced by ischemia-reperfusion (I/R) in Sprague-Dawley rats. On day
17 of gestation, leukocyte accumulation in the uterus and placenta
subjected to 30 min of ischemia, followed by reperfusion, was
assessed by measuring myeloperoxidase (MPO) activity. Uterine MPO
activity was significantly higher after 1 h of reperfusion than it
was before ischemia ( P < 0.05), without any
increase in placental MPO activity. Immunohistochemical staining showed
leukocyte accumulation in the uterus subjected to I/R. The effects of
treatment with monoclonal antibodies against CD11a (WT1) and CD18 (WT3)
at a dose of 0.8 mg/kg on uterine blood flow and IUGR were
investigated. Laser-Doppler flowmetry demonstrated that uterine
hypoperfusion at 2 h after ischemia (blood flow,
51.7 ± 1.2%; P < 0.01) was inhibited by WT1 and
WT3 treatment. I/R-induced IUGR at full term ( P < 0.05 vs. nonischemic horn) was prevented by WT1 and WT3 treatment on
day 17 . These results indicate that leukocyte accumulation may play an important role in the pathogenesis of uterine hypoperfusion and IUGR induced by I/R in pregnant rats.
uterine blood flow; CD11/CD18 integrin; rat</abstract><cop>United States</cop><pmid>11179066</pmid><doi>10.1152/ajpheart.2001.280.3.h1215</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0363-6135 |
| ispartof | American journal of physiology. Heart and circulatory physiology, 2001-03, Vol.280 (3), p.H1215-H1221 |
| issn | 0363-6135 1522-1539 |
| language | eng |
| recordid | cdi_pubmed_primary_11179066 |
| source | MEDLINE; American Physiological Society; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Antibodies, Monoclonal - pharmacology CD18 Antigens - immunology Female Fetal Growth Retardation - immunology Fetal Growth Retardation - pathology Fetal Growth Retardation - physiopathology Fetal Weight Leukocytes - physiology Lymphocyte Function-Associated Antigen-1 - immunology Organ Size Placenta - blood supply Placenta - immunology Placenta - pathology Pregnancy Rats Reperfusion Injury - immunology Reperfusion Injury - pathology Reperfusion Injury - physiopathology Uterus - blood supply Uterus - immunology Uterus - physiopathology |
| title | Role of leukocytes in uterine hypoperfusion and fetal growth retardation induced by ischemia-reperfusion |
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