Nucleus raphe obscurus modulates hypoglossal output of neonatal rat in vitro transverse brain stem slices
Respiratory Neuroscience Laboratory, Departments of Physiology and Anaesthesia, University of Toronto, Toronto, Ontario, Canada M5S 1A8 Nucleus raphé obscurus (NRo) modulates hypoglossal (XII) nerve motor output in the in vitro transverse brain stem slice of neonatal rats (1-5 days old); chemical ab...
Gespeichert in:
| Veröffentlicht in: | Journal of applied physiology (1985) 2001-01, Vol.90 (1), p.269-279 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 279 |
|---|---|
| container_issue | 1 |
| container_start_page | 269 |
| container_title | Journal of applied physiology (1985) |
| container_volume | 90 |
| creator | Peever, John H Necakov, Aleksandar Duffin, James |
| description | Respiratory Neuroscience Laboratory, Departments of Physiology and
Anaesthesia, University of Toronto, Toronto, Ontario, Canada M5S
1A8
Nucleus raphé
obscurus (NRo) modulates hypoglossal (XII) nerve motor output in the in
vitro transverse brain stem slice of neonatal rats (1-5 days old);
chemical ablation of NRo and its focal CO 2 acidification
modulated the bursting rhythm of XII nerves. We microinjected a 4.5 mM
solution of kainic acid into the NRo to disrupt cellular activity and
observed that XII nerve activity was temporarily abolished
( n = 10). We also microinjected CO 2 -acidified (pH = 6.00 ± 0.01) artificial
cerebrospinal fluid (aCSF) into the NRo ( n = 6), the
pre-Bötzinger complex (PBC) ( n = 6), as well as a
control region in the lateral tegmental field equidistant to NRo, PBC,
and the XII motor nuclei ( n = 12). CO 2
acidification of the control region had no effect on XII motor output.
CO 2 acidification of the NRo significantly
( P |
| doi_str_mv | 10.1152/jappl.2001.90.1.269 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmed_primary_11133919</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>82111111</sourcerecordid><originalsourceid>FETCH-LOGICAL-c432t-7ff8b76311f0d60527ae699acfb45fd832fa3ce01c4ddfa5723e89ddbfa551d63</originalsourceid><addsrcrecordid>eNp1kUmPEzEQhS0EYkLgFyAhCyQ4dfDS63E0YmaQRnAZzpbbS9qRO268DOTfUyFhERIn28_fq7LrIfSSkg2lDXu_k8viN4wQuhlA2rB2eIRWcMMq2hL6GK36riFV1_TdBXqW0g7Ium7oU3RBKeV8oMMKuU9FeVMSjnKZDA5jUiXCcQ66eJlNwtNhCVsfUpIeh5KXknGweG_CXmaQoszY7fGDyzHgHOU-PZiYDB6jBDllM-PknTLpOXpipU_mxXldoy_XH-6vbqu7zzcfry7vKlVzlqvO2n7sWk6pJbolDeukaYdBKjvWjdU9Z1ZyZQhVtdZWNh3jph-0HmHfUN3yNXp7qrvE8LWYlMXskjLeS3hzSaIjwEFZAF__A-5CiXt4m2CM0bZv-x4gfoJUhBFEY8US3SzjQVAijjGInzGIYwxiAElADOB6dS5dxtnoP57z3AF4cwZkUtJbmJty6Tc3NAODj63RuxM1ue30zUUjlumQXPBhezj2_bsh_z95Xby_N9_z0fLLIRZt-Q_IIrR_</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>222168688</pqid></control><display><type>article</type><title>Nucleus raphe obscurus modulates hypoglossal output of neonatal rat in vitro transverse brain stem slices</title><source>MEDLINE</source><source>American Physiological Society</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Peever, John H ; Necakov, Aleksandar ; Duffin, James</creator><creatorcontrib>Peever, John H ; Necakov, Aleksandar ; Duffin, James</creatorcontrib><description>Respiratory Neuroscience Laboratory, Departments of Physiology and
Anaesthesia, University of Toronto, Toronto, Ontario, Canada M5S
1A8
Nucleus raphé
obscurus (NRo) modulates hypoglossal (XII) nerve motor output in the in
vitro transverse brain stem slice of neonatal rats (1-5 days old);
chemical ablation of NRo and its focal CO 2 acidification
modulated the bursting rhythm of XII nerves. We microinjected a 4.5 mM
solution of kainic acid into the NRo to disrupt cellular activity and
observed that XII nerve activity was temporarily abolished
( n = 10). We also microinjected CO 2 -acidified (pH = 6.00 ± 0.01) artificial
cerebrospinal fluid (aCSF) into the NRo ( n = 6), the
pre-Bötzinger complex (PBC) ( n = 6), as well as a
control region in the lateral tegmental field equidistant to NRo, PBC,
and the XII motor nuclei ( n = 12). CO 2
acidification of the control region had no effect on XII motor output.
CO 2 acidification of the NRo significantly
( P < 0.05) increased the burst discharge frequency of
XII nerves by 77%; integrated burst amplitude and burst duration
increased by 64% and 52%, respectively. CO 2 acidification
of the PBC significantly ( P < 0.05) increased the
burst discharge frequency of XII nerves by 65%, but neither integrated
burst amplitude nor burst duration changed. These results demonstrate
that chemical ablation of the NRo can abolish XII nerve bursting rhythm
and that stimulation of the NRo with CO 2 -acidified aCSF can
excite XII nerve bursting activity. From these observations, we
conclude that, in transverse brain stem slices, the NRo contains
pH/CO 2 -sensitive cells that modulate XII motor output.
respiratory neurons; brain stem; pH/carbon dioxide</description><identifier>ISSN: 8750-7587</identifier><identifier>EISSN: 1522-1601</identifier><identifier>DOI: 10.1152/jappl.2001.90.1.269</identifier><identifier>PMID: 11133919</identifier><identifier>CODEN: JAPHEV</identifier><language>eng</language><publisher>Bethesda, MD: Am Physiological Soc</publisher><subject>Acids - metabolism ; Afferent Pathways - physiology ; Animals ; Animals, Newborn - physiology ; Biological and medical sciences ; Brain ; Brain Stem - physiology ; Carbon dioxide ; Carbon Dioxide - metabolism ; Fundamental and applied biological sciences. Psychology ; Hypoglossal Nerve - physiology ; In Vitro Techniques ; Kainic Acid - pharmacology ; Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration ; Neurons ; Raphe Nuclei - drug effects ; Raphe Nuclei - physiology ; Rats ; Rats, Sprague-Dawley ; Respiratory system ; Rodents ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of applied physiology (1985), 2001-01, Vol.90 (1), p.269-279</ispartof><rights>2001 INIST-CNRS</rights><rights>Copyright American Physiological Society Jan 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-7ff8b76311f0d60527ae699acfb45fd832fa3ce01c4ddfa5723e89ddbfa551d63</citedby><cites>FETCH-LOGICAL-c432t-7ff8b76311f0d60527ae699acfb45fd832fa3ce01c4ddfa5723e89ddbfa551d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3039,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=959272$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11133919$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peever, John H</creatorcontrib><creatorcontrib>Necakov, Aleksandar</creatorcontrib><creatorcontrib>Duffin, James</creatorcontrib><title>Nucleus raphe obscurus modulates hypoglossal output of neonatal rat in vitro transverse brain stem slices</title><title>Journal of applied physiology (1985)</title><addtitle>J Appl Physiol (1985)</addtitle><description>Respiratory Neuroscience Laboratory, Departments of Physiology and
Anaesthesia, University of Toronto, Toronto, Ontario, Canada M5S
1A8
Nucleus raphé
obscurus (NRo) modulates hypoglossal (XII) nerve motor output in the in
vitro transverse brain stem slice of neonatal rats (1-5 days old);
chemical ablation of NRo and its focal CO 2 acidification
modulated the bursting rhythm of XII nerves. We microinjected a 4.5 mM
solution of kainic acid into the NRo to disrupt cellular activity and
observed that XII nerve activity was temporarily abolished
( n = 10). We also microinjected CO 2 -acidified (pH = 6.00 ± 0.01) artificial
cerebrospinal fluid (aCSF) into the NRo ( n = 6), the
pre-Bötzinger complex (PBC) ( n = 6), as well as a
control region in the lateral tegmental field equidistant to NRo, PBC,
and the XII motor nuclei ( n = 12). CO 2
acidification of the control region had no effect on XII motor output.
CO 2 acidification of the NRo significantly
( P < 0.05) increased the burst discharge frequency of
XII nerves by 77%; integrated burst amplitude and burst duration
increased by 64% and 52%, respectively. CO 2 acidification
of the PBC significantly ( P < 0.05) increased the
burst discharge frequency of XII nerves by 65%, but neither integrated
burst amplitude nor burst duration changed. These results demonstrate
that chemical ablation of the NRo can abolish XII nerve bursting rhythm
and that stimulation of the NRo with CO 2 -acidified aCSF can
excite XII nerve bursting activity. From these observations, we
conclude that, in transverse brain stem slices, the NRo contains
pH/CO 2 -sensitive cells that modulate XII motor output.
respiratory neurons; brain stem; pH/carbon dioxide</description><subject>Acids - metabolism</subject><subject>Afferent Pathways - physiology</subject><subject>Animals</subject><subject>Animals, Newborn - physiology</subject><subject>Biological and medical sciences</subject><subject>Brain</subject><subject>Brain Stem - physiology</subject><subject>Carbon dioxide</subject><subject>Carbon Dioxide - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hypoglossal Nerve - physiology</subject><subject>In Vitro Techniques</subject><subject>Kainic Acid - pharmacology</subject><subject>Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration</subject><subject>Neurons</subject><subject>Raphe Nuclei - drug effects</subject><subject>Raphe Nuclei - physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Respiratory system</subject><subject>Rodents</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>8750-7587</issn><issn>1522-1601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUmPEzEQhS0EYkLgFyAhCyQ4dfDS63E0YmaQRnAZzpbbS9qRO268DOTfUyFhERIn28_fq7LrIfSSkg2lDXu_k8viN4wQuhlA2rB2eIRWcMMq2hL6GK36riFV1_TdBXqW0g7Ium7oU3RBKeV8oMMKuU9FeVMSjnKZDA5jUiXCcQ66eJlNwtNhCVsfUpIeh5KXknGweG_CXmaQoszY7fGDyzHgHOU-PZiYDB6jBDllM-PknTLpOXpipU_mxXldoy_XH-6vbqu7zzcfry7vKlVzlqvO2n7sWk6pJbolDeukaYdBKjvWjdU9Z1ZyZQhVtdZWNh3jph-0HmHfUN3yNXp7qrvE8LWYlMXskjLeS3hzSaIjwEFZAF__A-5CiXt4m2CM0bZv-x4gfoJUhBFEY8US3SzjQVAijjGInzGIYwxiAElADOB6dS5dxtnoP57z3AF4cwZkUtJbmJty6Tc3NAODj63RuxM1ue30zUUjlumQXPBhezj2_bsh_z95Xby_N9_z0fLLIRZt-Q_IIrR_</recordid><startdate>20010101</startdate><enddate>20010101</enddate><creator>Peever, John H</creator><creator>Necakov, Aleksandar</creator><creator>Duffin, James</creator><general>Am Physiological Soc</general><general>American Physiological Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TS</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20010101</creationdate><title>Nucleus raphe obscurus modulates hypoglossal output of neonatal rat in vitro transverse brain stem slices</title><author>Peever, John H ; Necakov, Aleksandar ; Duffin, James</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-7ff8b76311f0d60527ae699acfb45fd832fa3ce01c4ddfa5723e89ddbfa551d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Acids - metabolism</topic><topic>Afferent Pathways - physiology</topic><topic>Animals</topic><topic>Animals, Newborn - physiology</topic><topic>Biological and medical sciences</topic><topic>Brain</topic><topic>Brain Stem - physiology</topic><topic>Carbon dioxide</topic><topic>Carbon Dioxide - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hypoglossal Nerve - physiology</topic><topic>In Vitro Techniques</topic><topic>Kainic Acid - pharmacology</topic><topic>Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration</topic><topic>Neurons</topic><topic>Raphe Nuclei - drug effects</topic><topic>Raphe Nuclei - physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Respiratory system</topic><topic>Rodents</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peever, John H</creatorcontrib><creatorcontrib>Necakov, Aleksandar</creatorcontrib><creatorcontrib>Duffin, James</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied physiology (1985)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peever, John H</au><au>Necakov, Aleksandar</au><au>Duffin, James</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nucleus raphe obscurus modulates hypoglossal output of neonatal rat in vitro transverse brain stem slices</atitle><jtitle>Journal of applied physiology (1985)</jtitle><addtitle>J Appl Physiol (1985)</addtitle><date>2001-01-01</date><risdate>2001</risdate><volume>90</volume><issue>1</issue><spage>269</spage><epage>279</epage><pages>269-279</pages><issn>8750-7587</issn><eissn>1522-1601</eissn><coden>JAPHEV</coden><abstract>Respiratory Neuroscience Laboratory, Departments of Physiology and
Anaesthesia, University of Toronto, Toronto, Ontario, Canada M5S
1A8
Nucleus raphé
obscurus (NRo) modulates hypoglossal (XII) nerve motor output in the in
vitro transverse brain stem slice of neonatal rats (1-5 days old);
chemical ablation of NRo and its focal CO 2 acidification
modulated the bursting rhythm of XII nerves. We microinjected a 4.5 mM
solution of kainic acid into the NRo to disrupt cellular activity and
observed that XII nerve activity was temporarily abolished
( n = 10). We also microinjected CO 2 -acidified (pH = 6.00 ± 0.01) artificial
cerebrospinal fluid (aCSF) into the NRo ( n = 6), the
pre-Bötzinger complex (PBC) ( n = 6), as well as a
control region in the lateral tegmental field equidistant to NRo, PBC,
and the XII motor nuclei ( n = 12). CO 2
acidification of the control region had no effect on XII motor output.
CO 2 acidification of the NRo significantly
( P < 0.05) increased the burst discharge frequency of
XII nerves by 77%; integrated burst amplitude and burst duration
increased by 64% and 52%, respectively. CO 2 acidification
of the PBC significantly ( P < 0.05) increased the
burst discharge frequency of XII nerves by 65%, but neither integrated
burst amplitude nor burst duration changed. These results demonstrate
that chemical ablation of the NRo can abolish XII nerve bursting rhythm
and that stimulation of the NRo with CO 2 -acidified aCSF can
excite XII nerve bursting activity. From these observations, we
conclude that, in transverse brain stem slices, the NRo contains
pH/CO 2 -sensitive cells that modulate XII motor output.
respiratory neurons; brain stem; pH/carbon dioxide</abstract><cop>Bethesda, MD</cop><pub>Am Physiological Soc</pub><pmid>11133919</pmid><doi>10.1152/jappl.2001.90.1.269</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 8750-7587 |
| ispartof | Journal of applied physiology (1985), 2001-01, Vol.90 (1), p.269-279 |
| issn | 8750-7587 1522-1601 |
| language | eng |
| recordid | cdi_pubmed_primary_11133919 |
| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Acids - metabolism Afferent Pathways - physiology Animals Animals, Newborn - physiology Biological and medical sciences Brain Brain Stem - physiology Carbon dioxide Carbon Dioxide - metabolism Fundamental and applied biological sciences. Psychology Hypoglossal Nerve - physiology In Vitro Techniques Kainic Acid - pharmacology Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration Neurons Raphe Nuclei - drug effects Raphe Nuclei - physiology Rats Rats, Sprague-Dawley Respiratory system Rodents Vertebrates: nervous system and sense organs |
| title | Nucleus raphe obscurus modulates hypoglossal output of neonatal rat in vitro transverse brain stem slices |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T19%3A27%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nucleus%20raphe%20obscurus%20modulates%20hypoglossal%20output%20of%20neonatal%20rat%20in%20vitro%20transverse%20brain%20stem%20slices&rft.jtitle=Journal%20of%20applied%20physiology%20(1985)&rft.au=Peever,%20John%20H&rft.date=2001-01-01&rft.volume=90&rft.issue=1&rft.spage=269&rft.epage=279&rft.pages=269-279&rft.issn=8750-7587&rft.eissn=1522-1601&rft.coden=JAPHEV&rft_id=info:doi/10.1152/jappl.2001.90.1.269&rft_dat=%3Cproquest_pubme%3E82111111%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=222168688&rft_id=info:pmid/11133919&rfr_iscdi=true |