Molecular cloning and characterization of the human protein kinase D2. A novel member of the protein kinase D family of serine threonine kinases

We have isolated the full-length cDNA of a novel human serine threonine protein kinase gene. The deduced protein sequence contains two cysteine-rich motifs at the N terminus, a pleckstrin homology domain, and a catalytic domain containing all the characteristic sequence motifs of serine protein kina...

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Veröffentlicht in:The Journal of biological chemistry 2001-02, Vol.276 (5), p.3310
Hauptverfasser: Sturany, S, Van Lint, J, Muller, F, Wilda, M, Hameister, H, Hocker, M, Brey, A, Gern, U, Vandenheede, J, Gress, T, Adler, G, Seufferlein, T
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container_issue 5
container_start_page 3310
container_title The Journal of biological chemistry
container_volume 276
creator Sturany, S
Van Lint, J
Muller, F
Wilda, M
Hameister, H
Hocker, M
Brey, A
Gern, U
Vandenheede, J
Gress, T
Adler, G
Seufferlein, T
description We have isolated the full-length cDNA of a novel human serine threonine protein kinase gene. The deduced protein sequence contains two cysteine-rich motifs at the N terminus, a pleckstrin homology domain, and a catalytic domain containing all the characteristic sequence motifs of serine protein kinases. It exhibits the strongest homology to the serine threonine protein kinases PKD/PKCmicro and PKCnu, particularly in the duplex zinc finger-like cysteine-rich motif, in the pleckstrin homology domain and in the protein kinase domain. In contrast, it shows only a low degree of sequence similarity to other members of the PKC family. Therefore, the new protein has been termed protein kinase D2 (PKD2). The mRNA of PKD2 is widely expressed in human and murine tissues. It encodes a protein with a molecular mass of 105 kDa in SDS-polyacrylamide gel electrophoresis, which is expressed in various human cell lines, including HL60 cells, which do not express PKCmicro. In vivo phorbol ester binding studies demonstrated a concentration-dependent binding of [(3)H]phorbol 12,13-dibutyrate to PKD2. The addition of phorbol 12,13-dibutyrate in the presence of dioleoylphosphatidylserine stimulated the autophosphorylation of PKD2 in a synergistic fashion. Phorbol esters also stimulated autophosphorylation of PKD2 in intact cells. PKD2 activated by phorbol esters efficiently phosphorylated the exogenous substrate histone H1. In addition, we could identify the C-terminal Ser(876) residue as an in vivo phosphorylation site within PKD2. Phosphorylation of Ser(876) of PKD2 correlated with the activation status of the kinase. Finally, gastrin was found to be a physiological activator of PKD2 in human AGS-B cells stably transfected with the CCK(B)/gastrin receptor. Thus, PKD2 is a novel phorbol ester- and growth factor-stimulated protein kinase.
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Phorbol esters also stimulated autophosphorylation of PKD2 in intact cells. PKD2 activated by phorbol esters efficiently phosphorylated the exogenous substrate histone H1. In addition, we could identify the C-terminal Ser(876) residue as an in vivo phosphorylation site within PKD2. Phosphorylation of Ser(876) of PKD2 correlated with the activation status of the kinase. Finally, gastrin was found to be a physiological activator of PKD2 in human AGS-B cells stably transfected with the CCK(B)/gastrin receptor. 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A novel member of the protein kinase D family of serine threonine kinases</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2001-02-02</date><risdate>2001</risdate><volume>276</volume><issue>5</issue><spage>3310</spage><pages>3310-</pages><issn>0021-9258</issn><abstract>We have isolated the full-length cDNA of a novel human serine threonine protein kinase gene. The deduced protein sequence contains two cysteine-rich motifs at the N terminus, a pleckstrin homology domain, and a catalytic domain containing all the characteristic sequence motifs of serine protein kinases. It exhibits the strongest homology to the serine threonine protein kinases PKD/PKCmicro and PKCnu, particularly in the duplex zinc finger-like cysteine-rich motif, in the pleckstrin homology domain and in the protein kinase domain. In contrast, it shows only a low degree of sequence similarity to other members of the PKC family. 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source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection
subjects Amino Acid Sequence
Carcinogens - pharmacology
Cells, Cultured
Cloning, Molecular
DNA, Complementary - analysis
DNA, Complementary - genetics
Enzyme Activation
Growth Substances - pharmacology
HL-60 Cells
Humans
Molecular Sequence Data
Molecular Weight
Phorbol 12,13-Dibutyrate - pharmacology
Phorbol Esters - pharmacology
Phosphorylation
Protein Kinases - chemistry
Protein Kinases - drug effects
Protein Kinases - genetics
Protein Kinases - metabolism
Sequence Homology, Amino Acid
Serine - metabolism
Signal Transduction - physiology
Transfection
Tritium
title Molecular cloning and characterization of the human protein kinase D2. A novel member of the protein kinase D family of serine threonine kinases
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