Induction of BGT-1 and amino acid System A transport activities in endothelial cells exposed to hyperosmolarity
1 Dipartimento di Medicina Sperimentale, Sezione di Patologia Molecolare e Immunologia, Università degli Studi di Parma, 43100 Parma; 2 Centro Substrati Cellulari, Istituto Zooprofilattico Sperimentale, 25125 Brescia, Italy; and 3 School of Biological Sciences, University of Sussex, Brighton BN1...
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| Veröffentlicht in: | American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 2000-11, Vol.279 (5), p.1580-R1589 |
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| container_issue | 5 |
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| container_title | American journal of physiology. Regulatory, integrative and comparative physiology |
| container_volume | 279 |
| creator | Petronini, Pier-Giorgio Alfieri, Roberta R Losio, M. Nadia Caccamo, Alessandro E Cavazzoni, Andrea Bonelli, Mara A Borghetti, Angelo F Wheeler, Kenneth P |
| description | 1 Dipartimento di Medicina Sperimentale, Sezione di
Patologia Molecolare e Immunologia, Università degli Studi di
Parma, 43100 Parma; 2 Centro Substrati Cellulari, Istituto
Zooprofilattico Sperimentale, 25125 Brescia, Italy; and 3 School
of Biological Sciences, University of Sussex, Brighton BN1 9QG,
United Kingdom
We studied the
responses to hypertonicity of cultured endothelial cells from swine
pulmonary arteries. In 0.5 osmol/kgH 2 O medium, initial cell
shrinkage was followed by a regulatory volume increase (RVI), complete
after 1 h, concomitant with an increase in cellular K +
content. Then the activity of amino acid transport System A increased, accompanied by an accumulation of ninhydrin-positive solutes (NPS), reaching a peak at ~6 h. The subsequent decline in System A activity was paralleled by an induction of the betaine-GABA transporter (BGT-1),
detected as increases of BGT-1 mRNA and of transport activity, which
peaked at ~24 h. Inhibitors of transcription or translation prevented
induction of both transport activities. The increased expression of
BGT-1, which involved activation of "tonicity-responsive enhancer,"
was inhibited by 5 mM extracellular betaine. Cellular K +
concentration gradually declined after the accumulation of NPS and
during the induction of BGT-1. This very effective adaptation to
hypertonicity suggests it has a physiological role.
membrane transport; osmolyte; regulatory volume increase; volume |
| doi_str_mv | 10.1152/ajpregu.2000.279.5.r1580 |
| format | Article |
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Patologia Molecolare e Immunologia, Università degli Studi di
Parma, 43100 Parma; 2 Centro Substrati Cellulari, Istituto
Zooprofilattico Sperimentale, 25125 Brescia, Italy; and 3 School
of Biological Sciences, University of Sussex, Brighton BN1 9QG,
United Kingdom
We studied the
responses to hypertonicity of cultured endothelial cells from swine
pulmonary arteries. In 0.5 osmol/kgH 2 O medium, initial cell
shrinkage was followed by a regulatory volume increase (RVI), complete
after 1 h, concomitant with an increase in cellular K +
content. Then the activity of amino acid transport System A increased, accompanied by an accumulation of ninhydrin-positive solutes (NPS), reaching a peak at ~6 h. The subsequent decline in System A activity was paralleled by an induction of the betaine-GABA transporter (BGT-1),
detected as increases of BGT-1 mRNA and of transport activity, which
peaked at ~24 h. Inhibitors of transcription or translation prevented
induction of both transport activities. The increased expression of
BGT-1, which involved activation of "tonicity-responsive enhancer,"
was inhibited by 5 mM extracellular betaine. Cellular K +
concentration gradually declined after the accumulation of NPS and
during the induction of BGT-1. This very effective adaptation to
hypertonicity suggests it has a physiological role.
membrane transport; osmolyte; regulatory volume increase; volume</description><identifier>ISSN: 0363-6119</identifier><identifier>EISSN: 1522-1490</identifier><identifier>DOI: 10.1152/ajpregu.2000.279.5.r1580</identifier><identifier>PMID: 11049839</identifier><language>eng</language><publisher>United States</publisher><subject>Amino Acid Transport Systems ; Animals ; Betaine - metabolism ; Carrier Proteins - biosynthesis ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Cations ; Cell Line ; Cell Size ; Cells, Cultured ; Dogs ; Endothelium, Vascular - metabolism ; gamma-Aminobutyric Acid - metabolism ; Hypertonic Solutions ; Kinetics ; Ninhydrin - analysis ; Osmolar Concentration ; Potassium - metabolism ; Pulmonary Artery ; RNA, Messenger - analysis ; Sodium - metabolism ; Sodium Chloride - administration & dosage ; Sucrose - administration & dosage ; Swine</subject><ispartof>American journal of physiology. Regulatory, integrative and comparative physiology, 2000-11, Vol.279 (5), p.1580-R1589</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-9e61be44f8289b37b0e3294118e44de23d8d7f1290a4e6ec8b24f87b343a35713</citedby><cites>FETCH-LOGICAL-c467t-9e61be44f8289b37b0e3294118e44de23d8d7f1290a4e6ec8b24f87b343a35713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3026,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11049839$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Petronini, Pier-Giorgio</creatorcontrib><creatorcontrib>Alfieri, Roberta R</creatorcontrib><creatorcontrib>Losio, M. Nadia</creatorcontrib><creatorcontrib>Caccamo, Alessandro E</creatorcontrib><creatorcontrib>Cavazzoni, Andrea</creatorcontrib><creatorcontrib>Bonelli, Mara A</creatorcontrib><creatorcontrib>Borghetti, Angelo F</creatorcontrib><creatorcontrib>Wheeler, Kenneth P</creatorcontrib><title>Induction of BGT-1 and amino acid System A transport activities in endothelial cells exposed to hyperosmolarity</title><title>American journal of physiology. Regulatory, integrative and comparative physiology</title><addtitle>Am J Physiol Regul Integr Comp Physiol</addtitle><description>1 Dipartimento di Medicina Sperimentale, Sezione di
Patologia Molecolare e Immunologia, Università degli Studi di
Parma, 43100 Parma; 2 Centro Substrati Cellulari, Istituto
Zooprofilattico Sperimentale, 25125 Brescia, Italy; and 3 School
of Biological Sciences, University of Sussex, Brighton BN1 9QG,
United Kingdom
We studied the
responses to hypertonicity of cultured endothelial cells from swine
pulmonary arteries. In 0.5 osmol/kgH 2 O medium, initial cell
shrinkage was followed by a regulatory volume increase (RVI), complete
after 1 h, concomitant with an increase in cellular K +
content. Then the activity of amino acid transport System A increased, accompanied by an accumulation of ninhydrin-positive solutes (NPS), reaching a peak at ~6 h. The subsequent decline in System A activity was paralleled by an induction of the betaine-GABA transporter (BGT-1),
detected as increases of BGT-1 mRNA and of transport activity, which
peaked at ~24 h. Inhibitors of transcription or translation prevented
induction of both transport activities. The increased expression of
BGT-1, which involved activation of "tonicity-responsive enhancer,"
was inhibited by 5 mM extracellular betaine. Cellular K +
concentration gradually declined after the accumulation of NPS and
during the induction of BGT-1. This very effective adaptation to
hypertonicity suggests it has a physiological role.
membrane transport; osmolyte; regulatory volume increase; volume</description><subject>Amino Acid Transport Systems</subject><subject>Animals</subject><subject>Betaine - metabolism</subject><subject>Carrier Proteins - biosynthesis</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Cations</subject><subject>Cell Line</subject><subject>Cell Size</subject><subject>Cells, Cultured</subject><subject>Dogs</subject><subject>Endothelium, Vascular - metabolism</subject><subject>gamma-Aminobutyric Acid - metabolism</subject><subject>Hypertonic Solutions</subject><subject>Kinetics</subject><subject>Ninhydrin - analysis</subject><subject>Osmolar Concentration</subject><subject>Potassium - metabolism</subject><subject>Pulmonary Artery</subject><subject>RNA, Messenger - analysis</subject><subject>Sodium - metabolism</subject><subject>Sodium Chloride - administration & dosage</subject><subject>Sucrose - administration & dosage</subject><subject>Swine</subject><issn>0363-6119</issn><issn>1522-1490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1u1DAUhS0EokPhFZBX7BL8lx-zKxUtlSohwbC2nPhm4sqJg-1A8_Z4NAN0w8rS9Xeujz-EMCUlpRV7rx-WAIe1ZISQkjWyrMpAq5Y8Q7t8zQoqJHmOdoTXvKgplRfoVYwPGRZc8JfoglIiZMvlDvm72ax9sn7GfsAfb_cFxXo2WE929lj31uBvW0ww4Sucgp7j4kPK82R_2mQhYjtjmI1PIzirHe7BuYjhcfERDE4ej9sCwcfJOx1s2l6jF4N2Ed6cz0v0_ebT_vpzcf_l9u766r7oRd2kQkJNOxBiaFkrO950BDiTgtI2Dw0wblrTDJRJogXU0Lcdy2zT5e9pXjWUX6J3p71L8D9WiElNNh7L6Rn8GlXDsptsIYPtCexzyxhgUEuwkw6bokQdZauzbHWUrbJsVamvR9k5-vb8xtpNYP4Fz3Yz8OEEjPYw_rIB1DJu0XrnD5u6WZ3bw2P6s__JZrWYIYfL_4f_dnpS5zfO5qQv</recordid><startdate>20001101</startdate><enddate>20001101</enddate><creator>Petronini, Pier-Giorgio</creator><creator>Alfieri, Roberta R</creator><creator>Losio, M. Nadia</creator><creator>Caccamo, Alessandro E</creator><creator>Cavazzoni, Andrea</creator><creator>Bonelli, Mara A</creator><creator>Borghetti, Angelo F</creator><creator>Wheeler, Kenneth P</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20001101</creationdate><title>Induction of BGT-1 and amino acid System A transport activities in endothelial cells exposed to hyperosmolarity</title><author>Petronini, Pier-Giorgio ; Alfieri, Roberta R ; Losio, M. Nadia ; Caccamo, Alessandro E ; Cavazzoni, Andrea ; Bonelli, Mara A ; Borghetti, Angelo F ; Wheeler, Kenneth P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-9e61be44f8289b37b0e3294118e44de23d8d7f1290a4e6ec8b24f87b343a35713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Transport Systems</topic><topic>Animals</topic><topic>Betaine - metabolism</topic><topic>Carrier Proteins - biosynthesis</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Cations</topic><topic>Cell Line</topic><topic>Cell Size</topic><topic>Cells, Cultured</topic><topic>Dogs</topic><topic>Endothelium, Vascular - metabolism</topic><topic>gamma-Aminobutyric Acid - metabolism</topic><topic>Hypertonic Solutions</topic><topic>Kinetics</topic><topic>Ninhydrin - analysis</topic><topic>Osmolar Concentration</topic><topic>Potassium - metabolism</topic><topic>Pulmonary Artery</topic><topic>RNA, Messenger - analysis</topic><topic>Sodium - metabolism</topic><topic>Sodium Chloride - administration & dosage</topic><topic>Sucrose - administration & dosage</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Petronini, Pier-Giorgio</creatorcontrib><creatorcontrib>Alfieri, Roberta R</creatorcontrib><creatorcontrib>Losio, M. Nadia</creatorcontrib><creatorcontrib>Caccamo, Alessandro E</creatorcontrib><creatorcontrib>Cavazzoni, Andrea</creatorcontrib><creatorcontrib>Bonelli, Mara A</creatorcontrib><creatorcontrib>Borghetti, Angelo F</creatorcontrib><creatorcontrib>Wheeler, Kenneth P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Petronini, Pier-Giorgio</au><au>Alfieri, Roberta R</au><au>Losio, M. Nadia</au><au>Caccamo, Alessandro E</au><au>Cavazzoni, Andrea</au><au>Bonelli, Mara A</au><au>Borghetti, Angelo F</au><au>Wheeler, Kenneth P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of BGT-1 and amino acid System A transport activities in endothelial cells exposed to hyperosmolarity</atitle><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle><addtitle>Am J Physiol Regul Integr Comp Physiol</addtitle><date>2000-11-01</date><risdate>2000</risdate><volume>279</volume><issue>5</issue><spage>1580</spage><epage>R1589</epage><pages>1580-R1589</pages><issn>0363-6119</issn><eissn>1522-1490</eissn><abstract>1 Dipartimento di Medicina Sperimentale, Sezione di
Patologia Molecolare e Immunologia, Università degli Studi di
Parma, 43100 Parma; 2 Centro Substrati Cellulari, Istituto
Zooprofilattico Sperimentale, 25125 Brescia, Italy; and 3 School
of Biological Sciences, University of Sussex, Brighton BN1 9QG,
United Kingdom
We studied the
responses to hypertonicity of cultured endothelial cells from swine
pulmonary arteries. In 0.5 osmol/kgH 2 O medium, initial cell
shrinkage was followed by a regulatory volume increase (RVI), complete
after 1 h, concomitant with an increase in cellular K +
content. Then the activity of amino acid transport System A increased, accompanied by an accumulation of ninhydrin-positive solutes (NPS), reaching a peak at ~6 h. The subsequent decline in System A activity was paralleled by an induction of the betaine-GABA transporter (BGT-1),
detected as increases of BGT-1 mRNA and of transport activity, which
peaked at ~24 h. Inhibitors of transcription or translation prevented
induction of both transport activities. The increased expression of
BGT-1, which involved activation of "tonicity-responsive enhancer,"
was inhibited by 5 mM extracellular betaine. Cellular K +
concentration gradually declined after the accumulation of NPS and
during the induction of BGT-1. This very effective adaptation to
hypertonicity suggests it has a physiological role.
membrane transport; osmolyte; regulatory volume increase; volume</abstract><cop>United States</cop><pmid>11049839</pmid><doi>10.1152/ajpregu.2000.279.5.r1580</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0363-6119 |
| ispartof | American journal of physiology. Regulatory, integrative and comparative physiology, 2000-11, Vol.279 (5), p.1580-R1589 |
| issn | 0363-6119 1522-1490 |
| language | eng |
| recordid | cdi_pubmed_primary_11049839 |
| source | MEDLINE; American Physiological Society; EZB-FREE-00999 freely available EZB journals |
| subjects | Amino Acid Transport Systems Animals Betaine - metabolism Carrier Proteins - biosynthesis Carrier Proteins - genetics Carrier Proteins - metabolism Cations Cell Line Cell Size Cells, Cultured Dogs Endothelium, Vascular - metabolism gamma-Aminobutyric Acid - metabolism Hypertonic Solutions Kinetics Ninhydrin - analysis Osmolar Concentration Potassium - metabolism Pulmonary Artery RNA, Messenger - analysis Sodium - metabolism Sodium Chloride - administration & dosage Sucrose - administration & dosage Swine |
| title | Induction of BGT-1 and amino acid System A transport activities in endothelial cells exposed to hyperosmolarity |
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