Modified alkaline elution allows the measurement of intact apurinic sites in mammalian genomic DNA
The presence of apurinic/apyrimidinic (AP) sites in cell genomes is known to be toxic and mutagenic. These lesions are therefore repaired in cells by efficient enzymatic systems. However, a report (Nakamura and Swenberg, Cancer Res. 59 (1999) 2522-2526) indicates an unexpected high rate of endogenou...
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| Veröffentlicht in: | Biochimie 2000-08, Vol.82 (8), p.717 |
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| creator | Malvy, C Lefrançois, M Bertrand, J R Markovits, J |
| description | The presence of apurinic/apyrimidinic (AP) sites in cell genomes is known to be toxic and mutagenic. These lesions are therefore repaired in cells by efficient enzymatic systems. However, a report (Nakamura and Swenberg, Cancer Res. 59 (1999) 2522-2526) indicates an unexpected high rate of endogenous apurinic/apyrimidinic (AP) sites in genomic DNA in mammalian tissues. The technology used does not allow the authors to distinguish between intact AP sites and 3'cleaved AP sites. The corresponding values range between 2 and 4 sites per million of nucleotides in various human and rat tissues. Using a modified alkaline elution method we show here that the stationary level of intact AP sites is about 0.16 per million of nucleotides in leukemic mouse L1210 cells. |
| doi_str_mv | 10.1016/S0300-9084(00)01152-4 |
| format | Article |
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These lesions are therefore repaired in cells by efficient enzymatic systems. However, a report (Nakamura and Swenberg, Cancer Res. 59 (1999) 2522-2526) indicates an unexpected high rate of endogenous apurinic/apyrimidinic (AP) sites in genomic DNA in mammalian tissues. The technology used does not allow the authors to distinguish between intact AP sites and 3'cleaved AP sites. The corresponding values range between 2 and 4 sites per million of nucleotides in various human and rat tissues. Using a modified alkaline elution method we show here that the stationary level of intact AP sites is about 0.16 per million of nucleotides in leukemic mouse L1210 cells.</description><identifier>ISSN: 0300-9084</identifier><identifier>DOI: 10.1016/S0300-9084(00)01152-4</identifier><identifier>PMID: 11018287</identifier><language>eng</language><publisher>France</publisher><subject>Animals ; Apurinic Acid - chemistry ; Apurinic Acid - isolation & purification ; DNA - chemistry ; DNA - isolation & purification ; DNA Damage ; DNA, Neoplasm - chemistry ; DNA, Neoplasm - isolation & purification ; Humans ; Hydrogen-Ion Concentration ; Kinetics ; Leukemia L1210 - metabolism ; Leukemia L1210 - pathology ; Mammals ; Mice ; Oligopeptides ; Rats ; Tumor Cells, Cultured</subject><ispartof>Biochimie, 2000-08, Vol.82 (8), p.717</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11018287$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Malvy, C</creatorcontrib><creatorcontrib>Lefrançois, M</creatorcontrib><creatorcontrib>Bertrand, J R</creatorcontrib><creatorcontrib>Markovits, J</creatorcontrib><title>Modified alkaline elution allows the measurement of intact apurinic sites in mammalian genomic DNA</title><title>Biochimie</title><addtitle>Biochimie</addtitle><description>The presence of apurinic/apyrimidinic (AP) sites in cell genomes is known to be toxic and mutagenic. These lesions are therefore repaired in cells by efficient enzymatic systems. However, a report (Nakamura and Swenberg, Cancer Res. 59 (1999) 2522-2526) indicates an unexpected high rate of endogenous apurinic/apyrimidinic (AP) sites in genomic DNA in mammalian tissues. The technology used does not allow the authors to distinguish between intact AP sites and 3'cleaved AP sites. The corresponding values range between 2 and 4 sites per million of nucleotides in various human and rat tissues. Using a modified alkaline elution method we show here that the stationary level of intact AP sites is about 0.16 per million of nucleotides in leukemic mouse L1210 cells.</description><subject>Animals</subject><subject>Apurinic Acid - chemistry</subject><subject>Apurinic Acid - isolation & purification</subject><subject>DNA - chemistry</subject><subject>DNA - isolation & purification</subject><subject>DNA Damage</subject><subject>DNA, Neoplasm - chemistry</subject><subject>DNA, Neoplasm - isolation & purification</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kinetics</subject><subject>Leukemia L1210 - metabolism</subject><subject>Leukemia L1210 - pathology</subject><subject>Mammals</subject><subject>Mice</subject><subject>Oligopeptides</subject><subject>Rats</subject><subject>Tumor Cells, Cultured</subject><issn>0300-9084</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9j81KxDAYRbNQnHH0EZQsdVH9krRNshz8h1EX6npIk68abdLSpIhvb8Gf1YVzuQcuIUcMzhiw-vwJBEChQZUnAKfAWMWLcocs__GC7Kf0DgAVcL1HFmyeKa7kkjT3vfOtR0dN92E6H5FiN2Xfxxl0_Wei-Q1pQJOmEQPGTPuW-piNzdQM0-ijtzT5jGmmNJgQZomJ9BVjH-bq8mF9QHZb0yU8_M0Vebm-er64LTaPN3cX600xcJC5ENxpCQqNrrmTVhsm6tqAlI2EphJQ8lpYUSGTDq2qFRitoOLOtbashNNiRY5_vMPUBHTbYfTBjF_bv7PiG3nZVdE</recordid><startdate>20000801</startdate><enddate>20000801</enddate><creator>Malvy, C</creator><creator>Lefrançois, M</creator><creator>Bertrand, J R</creator><creator>Markovits, J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20000801</creationdate><title>Modified alkaline elution allows the measurement of intact apurinic sites in mammalian genomic DNA</title><author>Malvy, C ; Lefrançois, M ; Bertrand, J R ; Markovits, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p207t-32d9708ea962d7c9a1366a077b70b5304263c35e17dec8680a98052ddfc453d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Apurinic Acid - chemistry</topic><topic>Apurinic Acid - isolation & purification</topic><topic>DNA - chemistry</topic><topic>DNA - isolation & purification</topic><topic>DNA Damage</topic><topic>DNA, Neoplasm - chemistry</topic><topic>DNA, Neoplasm - isolation & purification</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Kinetics</topic><topic>Leukemia L1210 - metabolism</topic><topic>Leukemia L1210 - pathology</topic><topic>Mammals</topic><topic>Mice</topic><topic>Oligopeptides</topic><topic>Rats</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Malvy, C</creatorcontrib><creatorcontrib>Lefrançois, M</creatorcontrib><creatorcontrib>Bertrand, J R</creatorcontrib><creatorcontrib>Markovits, J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Biochimie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Malvy, C</au><au>Lefrançois, M</au><au>Bertrand, J R</au><au>Markovits, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modified alkaline elution allows the measurement of intact apurinic sites in mammalian genomic DNA</atitle><jtitle>Biochimie</jtitle><addtitle>Biochimie</addtitle><date>2000-08-01</date><risdate>2000</risdate><volume>82</volume><issue>8</issue><spage>717</spage><pages>717-</pages><issn>0300-9084</issn><abstract>The presence of apurinic/apyrimidinic (AP) sites in cell genomes is known to be toxic and mutagenic. 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| ispartof | Biochimie, 2000-08, Vol.82 (8), p.717 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Apurinic Acid - chemistry Apurinic Acid - isolation & purification DNA - chemistry DNA - isolation & purification DNA Damage DNA, Neoplasm - chemistry DNA, Neoplasm - isolation & purification Humans Hydrogen-Ion Concentration Kinetics Leukemia L1210 - metabolism Leukemia L1210 - pathology Mammals Mice Oligopeptides Rats Tumor Cells, Cultured |
| title | Modified alkaline elution allows the measurement of intact apurinic sites in mammalian genomic DNA |
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