RIC-8 (Synembryn): a novel conserved protein that is required for G(q)alpha signaling in the C. elegans nervous system
Recent studies describe a network of signaling proteins centered around G(o)alpha and G(q)alpha that regulates neurotransmitter secretion in C. elegans by controlling the production and consumption of diacylglycerol (DAG). We sought other components of the Goalpha-G(q)alpha signaling network by scre...
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| Veröffentlicht in: | Neuron (Cambridge, Mass.) Mass.), 2000-08, Vol.27 (2), p.289 |
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| creator | Miller, K G Emerson, M D McManus, J R Rand, J B |
| description | Recent studies describe a network of signaling proteins centered around G(o)alpha and G(q)alpha that regulates neurotransmitter secretion in C. elegans by controlling the production and consumption of diacylglycerol (DAG). We sought other components of the Goalpha-G(q)alpha signaling network by screening for aldicarb-resistant mutants with phenotypes similar to egl-30 (G(q)alpha) mutants. In so doing, we identified ric-8, which encodes a novel protein named RIC-8 (synembryn). Through cDNA analysis, we show that RIC-8 is conserved in vertebrates. Through immunostaining, we show that RIC-8 is concentrated in the cytoplasm of neurons. Exogenous application of phorbol esters or loss of DGK-1 (diacylglycerol kinase) rescues ric-8 mutant phenotypes. A genetic analysis suggests that RIC-8 functions upstream of, or in conjunction with, EGL-30 (G(q)alpha). |
| doi_str_mv | 10.1016/S0896-6273(00)00037-4 |
| format | Article |
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We sought other components of the Goalpha-G(q)alpha signaling network by screening for aldicarb-resistant mutants with phenotypes similar to egl-30 (G(q)alpha) mutants. In so doing, we identified ric-8, which encodes a novel protein named RIC-8 (synembryn). Through cDNA analysis, we show that RIC-8 is conserved in vertebrates. Through immunostaining, we show that RIC-8 is concentrated in the cytoplasm of neurons. Exogenous application of phorbol esters or loss of DGK-1 (diacylglycerol kinase) rescues ric-8 mutant phenotypes. A genetic analysis suggests that RIC-8 functions upstream of, or in conjunction with, EGL-30 (G(q)alpha).</description><identifier>ISSN: 0896-6273</identifier><identifier>DOI: 10.1016/S0896-6273(00)00037-4</identifier><identifier>PMID: 10985349</identifier><language>eng</language><publisher>United States</publisher><subject>Aging - metabolism ; Animals ; Caenorhabditis elegans - genetics ; Caenorhabditis elegans - metabolism ; Caenorhabditis elegans Proteins ; Conserved Sequence - genetics ; Diacylglycerol Kinase - deficiency ; Diacylglycerol Kinase - genetics ; Fluorescent Antibody Technique ; GTP-Binding Protein alpha Subunits, Gq-G11 ; GTP-Binding Proteins - metabolism ; Guanine Nucleotide Exchange Factors ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Nervous System - metabolism ; Nuclear Proteins - genetics ; Nuclear Proteins - metabolism ; Organ Specificity ; Phenotype ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Signal Transduction - physiology ; Synaptic Transmission - genetics ; Tetradecanoylphorbol Acetate - pharmacology</subject><ispartof>Neuron (Cambridge, Mass.), 2000-08, Vol.27 (2), p.289</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c259t-a01cd588c47a354952f5d1f19756305c2233e954f4d54cf3da506d980c9433283</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10985349$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miller, K G</creatorcontrib><creatorcontrib>Emerson, M D</creatorcontrib><creatorcontrib>McManus, J R</creatorcontrib><creatorcontrib>Rand, J B</creatorcontrib><title>RIC-8 (Synembryn): a novel conserved protein that is required for G(q)alpha signaling in the C. elegans nervous system</title><title>Neuron (Cambridge, Mass.)</title><addtitle>Neuron</addtitle><description>Recent studies describe a network of signaling proteins centered around G(o)alpha and G(q)alpha that regulates neurotransmitter secretion in C. elegans by controlling the production and consumption of diacylglycerol (DAG). We sought other components of the Goalpha-G(q)alpha signaling network by screening for aldicarb-resistant mutants with phenotypes similar to egl-30 (G(q)alpha) mutants. In so doing, we identified ric-8, which encodes a novel protein named RIC-8 (synembryn). Through cDNA analysis, we show that RIC-8 is conserved in vertebrates. Through immunostaining, we show that RIC-8 is concentrated in the cytoplasm of neurons. Exogenous application of phorbol esters or loss of DGK-1 (diacylglycerol kinase) rescues ric-8 mutant phenotypes. A genetic analysis suggests that RIC-8 functions upstream of, or in conjunction with, EGL-30 (G(q)alpha).</description><subject>Aging - metabolism</subject><subject>Animals</subject><subject>Caenorhabditis elegans - genetics</subject><subject>Caenorhabditis elegans - metabolism</subject><subject>Caenorhabditis elegans Proteins</subject><subject>Conserved Sequence - genetics</subject><subject>Diacylglycerol Kinase - deficiency</subject><subject>Diacylglycerol Kinase - genetics</subject><subject>Fluorescent Antibody Technique</subject><subject>GTP-Binding Protein alpha Subunits, Gq-G11</subject><subject>GTP-Binding Proteins - metabolism</subject><subject>Guanine Nucleotide Exchange Factors</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis, Site-Directed</subject><subject>Nervous System - metabolism</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>Organ Specificity</subject><subject>Phenotype</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Signal Transduction - physiology</subject><subject>Synaptic Transmission - genetics</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><issn>0896-6273</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtOwzAURL0A0fL4BNBdtouU69hObHYoglKpEhKFdeXaThuUOKmdVsrfU_FajTRzdBZDyC3FGUWa3a9QqizJ0pxNEKeIyPKEn5Hxfz0ilzF-IlIuFL0gI4pKCsbVmBzfFkUiYbIavGs2YfDTB9Dg26OrwbQ-unB0FrrQ9q7y0O90D1WE4PaHKpyGsg0wn-ynuu52GmK19bqu_Ba-WQfFDFztttpH8CdTe4gQh9i75pqcl7qO7uY3r8jH89N78ZIsX-eL4nGZmFSoPtFIjRVSGp5rJrgSaSksLanKRcZQmDRlzCnBS24FNyWzWmBmlUSjOGOpZFfk7sfbHTaNs-suVI0Ow_rvAPYFFUxbUQ</recordid><startdate>20000801</startdate><enddate>20000801</enddate><creator>Miller, K G</creator><creator>Emerson, M D</creator><creator>McManus, J R</creator><creator>Rand, J B</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20000801</creationdate><title>RIC-8 (Synembryn): a novel conserved protein that is required for G(q)alpha signaling in the C. elegans nervous system</title><author>Miller, K G ; Emerson, M D ; McManus, J R ; Rand, J B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c259t-a01cd588c47a354952f5d1f19756305c2233e954f4d54cf3da506d980c9433283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Aging - metabolism</topic><topic>Animals</topic><topic>Caenorhabditis elegans - genetics</topic><topic>Caenorhabditis elegans - metabolism</topic><topic>Caenorhabditis elegans Proteins</topic><topic>Conserved Sequence - genetics</topic><topic>Diacylglycerol Kinase - deficiency</topic><topic>Diacylglycerol Kinase - genetics</topic><topic>Fluorescent Antibody Technique</topic><topic>GTP-Binding Protein alpha Subunits, Gq-G11</topic><topic>GTP-Binding Proteins - metabolism</topic><topic>Guanine Nucleotide Exchange Factors</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis, Site-Directed</topic><topic>Nervous System - metabolism</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>Organ Specificity</topic><topic>Phenotype</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Signal Transduction - physiology</topic><topic>Synaptic Transmission - genetics</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miller, K G</creatorcontrib><creatorcontrib>Emerson, M D</creatorcontrib><creatorcontrib>McManus, J R</creatorcontrib><creatorcontrib>Rand, J B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Neuron (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miller, K G</au><au>Emerson, M D</au><au>McManus, J R</au><au>Rand, J B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RIC-8 (Synembryn): a novel conserved protein that is required for G(q)alpha signaling in the C. elegans nervous system</atitle><jtitle>Neuron (Cambridge, Mass.)</jtitle><addtitle>Neuron</addtitle><date>2000-08-01</date><risdate>2000</risdate><volume>27</volume><issue>2</issue><spage>289</spage><pages>289-</pages><issn>0896-6273</issn><abstract>Recent studies describe a network of signaling proteins centered around G(o)alpha and G(q)alpha that regulates neurotransmitter secretion in C. elegans by controlling the production and consumption of diacylglycerol (DAG). We sought other components of the Goalpha-G(q)alpha signaling network by screening for aldicarb-resistant mutants with phenotypes similar to egl-30 (G(q)alpha) mutants. In so doing, we identified ric-8, which encodes a novel protein named RIC-8 (synembryn). Through cDNA analysis, we show that RIC-8 is conserved in vertebrates. Through immunostaining, we show that RIC-8 is concentrated in the cytoplasm of neurons. Exogenous application of phorbol esters or loss of DGK-1 (diacylglycerol kinase) rescues ric-8 mutant phenotypes. A genetic analysis suggests that RIC-8 functions upstream of, or in conjunction with, EGL-30 (G(q)alpha).</abstract><cop>United States</cop><pmid>10985349</pmid><doi>10.1016/S0896-6273(00)00037-4</doi></addata></record> |
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Aging - metabolism Animals Caenorhabditis elegans - genetics Caenorhabditis elegans - metabolism Caenorhabditis elegans Proteins Conserved Sequence - genetics Diacylglycerol Kinase - deficiency Diacylglycerol Kinase - genetics Fluorescent Antibody Technique GTP-Binding Protein alpha Subunits, Gq-G11 GTP-Binding Proteins - metabolism Guanine Nucleotide Exchange Factors Molecular Sequence Data Mutagenesis, Site-Directed Nervous System - metabolism Nuclear Proteins - genetics Nuclear Proteins - metabolism Organ Specificity Phenotype Sequence Analysis, DNA Sequence Homology, Amino Acid Signal Transduction - physiology Synaptic Transmission - genetics Tetradecanoylphorbol Acetate - pharmacology |
| title | RIC-8 (Synembryn): a novel conserved protein that is required for G(q)alpha signaling in the C. elegans nervous system |
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