An Endogenous Inhibitor of Ca++-ATPase from Human Placenta
Intracellular free calcium is regulated by Ca++-ATPase, one form present on the plasma membrane (PM Ca++-ATPase) and the other on sarcoplasmic (endoplasmic) reticulum (SR/ER Ca++-ATPase). An endogenous inhibitor of SR Ca++-ATPase from human placenta was shown to be present in normal placenta and the...
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| Veröffentlicht in: | Journal of enzyme inhibition 2000, Vol.15 (2), p.163-170 |
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| container_title | Journal of enzyme inhibition |
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| creator | Javed, Masood-Ul-Hassan Naru, Tahira Yasmeen Michelangeli, Francesco |
| description | Intracellular free calcium is regulated by Ca++-ATPase, one form present on the plasma membrane (PM Ca++-ATPase) and the other on sarcoplasmic (endoplasmic) reticulum (SR/ER Ca++-ATPase). An endogenous inhibitor of SR Ca++-ATPase from human placenta was shown to be present in normal placenta and the activity was not detectable in placenta from preeclamptic patients. The inhibitor was distributed in cytosol and microsomes. The inhibition of Ca++-ATPase by this inhibitor was concentration-and time-dependent. The inhibitor neither bound to DEAE-nor CM-sepharose resins at pH 7.5 and 8.5. Furthermore, it was heat stable for 15min up to 55°C and completely destroyed at 80°C in a few minutes. It was also observed to be stable at room temperature for at least 3 months. The purification and characterization of this inhibitor would be valuable in achieving an understanding of the normal regulation of Ca++-ATPase in the placenta during pregnancy. |
| doi_str_mv | 10.1080/14756360009030348 |
| format | Article |
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An endogenous inhibitor of SR Ca++-ATPase from human placenta was shown to be present in normal placenta and the activity was not detectable in placenta from preeclamptic patients. The inhibitor was distributed in cytosol and microsomes. The inhibition of Ca++-ATPase by this inhibitor was concentration-and time-dependent. The inhibitor neither bound to DEAE-nor CM-sepharose resins at pH 7.5 and 8.5. Furthermore, it was heat stable for 15min up to 55°C and completely destroyed at 80°C in a few minutes. It was also observed to be stable at room temperature for at least 3 months. 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An endogenous inhibitor of SR Ca++-ATPase from human placenta was shown to be present in normal placenta and the activity was not detectable in placenta from preeclamptic patients. The inhibitor was distributed in cytosol and microsomes. The inhibition of Ca++-ATPase by this inhibitor was concentration-and time-dependent. The inhibitor neither bound to DEAE-nor CM-sepharose resins at pH 7.5 and 8.5. Furthermore, it was heat stable for 15min up to 55°C and completely destroyed at 80°C in a few minutes. It was also observed to be stable at room temperature for at least 3 months. The purification and characterization of this inhibitor would be valuable in achieving an understanding of the normal regulation of Ca++-ATPase in the placenta during pregnancy.</description><subject>Animals</subject><subject>ATPase</subject><subject>Calcium-Transporting ATPases - antagonists & inhibitors</subject><subject>Cytosol - metabolism</subject><subject>Enzyme Inhibitors - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>Inhibitor</subject><subject>Microsomes - metabolism</subject><subject>Placenta</subject><subject>Placenta - metabolism</subject><subject>Pregnancy</subject><subject>Rabbits</subject><subject>Temperature</subject><issn>1475-6366</issn><issn>8755-5093</issn><issn>1475-6374</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1LwzAYhYMobk5_gDfSK29GNV_9Um_GmDoYuIt5Hd6mietok5m0yP69HR2iCLvKS3jO4fAgdE3wHcEpvic8iWIWY4wzzDDj6Qka7v_CmCX89OeO4wG68H6DMSWU8HM0IDhjacTJED1MTDAzhf1QxrY-mJt1mZeNdYHVwRTG43CyWoJXgXa2Dl7bGkywrEAq08AlOtNQeXV1eEfo_Xm2mr6Gi7eX-XSyCCXnUROyKJcU50WRywiwzBiFImFpN1qyiKRRRrSmHLK8UClRPFMk1VoRRqjiCSMxG6Hbvnfr7GerfCPq0ktVVWBUt1kkNKIZT3gHkh6UznrvlBZbV9bgdoJgsRcm_gnrMjeH8javVfEr0RvqgKceKI22roYv66pCNLCrrNMOjCy9YMf6H__E1wqqZi3BKbGxrTOduCPrvgGPg4hx</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>Javed, Masood-Ul-Hassan</creator><creator>Naru, Tahira Yasmeen</creator><creator>Michelangeli, Francesco</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2000</creationdate><title>An Endogenous Inhibitor of Ca++-ATPase from Human Placenta</title><author>Javed, Masood-Ul-Hassan ; Naru, Tahira Yasmeen ; Michelangeli, Francesco</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-35bc20bddbc5a0c932ad738000c3518591ff24a9bde81e49e18ffe1312e473163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>ATPase</topic><topic>Calcium-Transporting ATPases - antagonists & inhibitors</topic><topic>Cytosol - metabolism</topic><topic>Enzyme Inhibitors - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>Inhibitor</topic><topic>Microsomes - metabolism</topic><topic>Placenta</topic><topic>Placenta - metabolism</topic><topic>Pregnancy</topic><topic>Rabbits</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Javed, Masood-Ul-Hassan</creatorcontrib><creatorcontrib>Naru, Tahira Yasmeen</creatorcontrib><creatorcontrib>Michelangeli, Francesco</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of enzyme inhibition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Javed, Masood-Ul-Hassan</au><au>Naru, Tahira Yasmeen</au><au>Michelangeli, Francesco</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An Endogenous Inhibitor of Ca++-ATPase from Human Placenta</atitle><jtitle>Journal of enzyme inhibition</jtitle><addtitle>J Enzyme Inhib</addtitle><date>2000</date><risdate>2000</risdate><volume>15</volume><issue>2</issue><spage>163</spage><epage>170</epage><pages>163-170</pages><issn>1475-6366</issn><issn>8755-5093</issn><eissn>1475-6374</eissn><abstract>Intracellular free calcium is regulated by Ca++-ATPase, one form present on the plasma membrane (PM Ca++-ATPase) and the other on sarcoplasmic (endoplasmic) reticulum (SR/ER Ca++-ATPase). An endogenous inhibitor of SR Ca++-ATPase from human placenta was shown to be present in normal placenta and the activity was not detectable in placenta from preeclamptic patients. The inhibitor was distributed in cytosol and microsomes. The inhibition of Ca++-ATPase by this inhibitor was concentration-and time-dependent. The inhibitor neither bound to DEAE-nor CM-sepharose resins at pH 7.5 and 8.5. Furthermore, it was heat stable for 15min up to 55°C and completely destroyed at 80°C in a few minutes. It was also observed to be stable at room temperature for at least 3 months. The purification and characterization of this inhibitor would be valuable in achieving an understanding of the normal regulation of Ca++-ATPase in the placenta during pregnancy.</abstract><cop>Switzerland</cop><pub>Informa UK Ltd</pub><pmid>10938541</pmid><doi>10.1080/14756360009030348</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 1475-6366 8755-5093 1475-6374 |
| language | eng |
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| source | Taylor & Francis Open Access |
| subjects | Animals ATPase Calcium-Transporting ATPases - antagonists & inhibitors Cytosol - metabolism Enzyme Inhibitors - metabolism Female Humans Inhibitor Microsomes - metabolism Placenta Placenta - metabolism Pregnancy Rabbits Temperature |
| title | An Endogenous Inhibitor of Ca++-ATPase from Human Placenta |
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