DRM/GREMLIN (CKTSF1B1) maps to human chromosome 15 and is highly expressed in adult and fetal brain
Abstract. We have mapped and characterized the human homolog of Drm/Gremlin (CKTFS1B1), a member of a family of BMP antagonists that have been linked to both developmental and transformation-related functions. By screening a human cDNA library, we isolated a 3.3-kb cDNA containing the 552-bp region...
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| Veröffentlicht in: | Cytogenetic and genome research 2000-01, Vol.89 (1-2), p.79-84 |
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| creator | Topol, L.Z. Modi, W.S. Koochekpour, S. Blair, D.G. |
| description | Abstract. We have mapped and characterized the human homolog of Drm/Gremlin (CKTFS1B1), a member of a family of BMP antagonists that have been linked to both developmental and transformation-related functions. By screening a human cDNA library, we isolated a 3.3-kb cDNA containing the 552-bp region encoding the human DRM protein. CKTFS1B1 was localized on human chromosome 15q13→ q15 by somatic cell hybrid analysis and, more precisely, using radiation hybrids, to a region of markers linked to SGNE1, secretory granule neuroendocrine protein 1 and RYR3, the ryanodyne receptor 3. Northern blot analysis showed the presence of a single DRM-specific mRNA expressed in different human tissues, including brain, ovary, intestine and colon. In the brain, DRM expression is associated with the region localized around the internal capsule in the large subcortical nuclei. DRM appears to be predominantly expressed in normal cells and tissues, including normal neurons, astrocytes and fibroblasts. Interestingly, we detected DRM expression in normal cells obtained from several patients, but not in tumor cell lines established from the same patients. The data suggest that down-regulation of DRM is associated with tumor progression, and support the hypothesis that human DRM may play an important role during both neuroembryological development and carcinogenesis. |
| doi_str_mv | 10.1159/000015568 |
| format | Article |
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We have mapped and characterized the human homolog of Drm/Gremlin (CKTFS1B1), a member of a family of BMP antagonists that have been linked to both developmental and transformation-related functions. By screening a human cDNA library, we isolated a 3.3-kb cDNA containing the 552-bp region encoding the human DRM protein. CKTFS1B1 was localized on human chromosome 15q13→ q15 by somatic cell hybrid analysis and, more precisely, using radiation hybrids, to a region of markers linked to SGNE1, secretory granule neuroendocrine protein 1 and RYR3, the ryanodyne receptor 3. Northern blot analysis showed the presence of a single DRM-specific mRNA expressed in different human tissues, including brain, ovary, intestine and colon. In the brain, DRM expression is associated with the region localized around the internal capsule in the large subcortical nuclei. DRM appears to be predominantly expressed in normal cells and tissues, including normal neurons, astrocytes and fibroblasts. Interestingly, we detected DRM expression in normal cells obtained from several patients, but not in tumor cell lines established from the same patients. The data suggest that down-regulation of DRM is associated with tumor progression, and support the hypothesis that human DRM may play an important role during both neuroembryological development and carcinogenesis. </description><identifier>ISSN: 1424-8581</identifier><identifier>ISSN: 0301-0171</identifier><identifier>EISSN: 1424-859X</identifier><identifier>DOI: 10.1159/000015568</identifier><identifier>PMID: 10894942</identifier><identifier>CODEN: CGCGBR</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Aging - genetics ; Aging - metabolism ; Biological and medical sciences ; Bone Morphogenetic Proteins - metabolism ; Brain - cytology ; Brain - embryology ; Brain - growth & development ; Brain - metabolism ; Cell Line ; chromosome 15 ; Chromosomes, Human, Pair 15 - genetics ; CKTSF1B1 gene ; Cloning, Molecular ; DRM/GREMLIN protein ; Fundamental and applied biological sciences. Psychology ; Gene Expression Profiling ; Genes. Genome ; Genetic Linkage - genetics ; Humans ; Hybrid Cells ; Intercellular Signaling Peptides and Proteins ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Neoplasms - genetics ; Neoplasms - metabolism ; Neoplasms - pathology ; Physical Chromosome Mapping ; Proteins - genetics ; Proteins - metabolism ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; Tumor Cells, Cultured</subject><ispartof>Cytogenetic and genome research, 2000-01, Vol.89 (1-2), p.79-84</ispartof><rights>2000 S. Karger AG, Basel</rights><rights>2000 INIST-CNRS</rights><rights>Copyright 2000 S. Karger AG, Basel.</rights><rights>Copyright S. Karger AG 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-113b70478855c7988ed84b58bbb6856ec3c212961cce8457dcfaa2ccf4f85de53</citedby><cites>FETCH-LOGICAL-c415t-113b70478855c7988ed84b58bbb6856ec3c212961cce8457dcfaa2ccf4f85de53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2423,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1422587$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10894942$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Topol, L.Z.</creatorcontrib><creatorcontrib>Modi, W.S.</creatorcontrib><creatorcontrib>Koochekpour, S.</creatorcontrib><creatorcontrib>Blair, D.G.</creatorcontrib><title>DRM/GREMLIN (CKTSF1B1) maps to human chromosome 15 and is highly expressed in adult and fetal brain</title><title>Cytogenetic and genome research</title><addtitle>Cytogenet Genome Res</addtitle><description>Abstract. We have mapped and characterized the human homolog of Drm/Gremlin (CKTFS1B1), a member of a family of BMP antagonists that have been linked to both developmental and transformation-related functions. By screening a human cDNA library, we isolated a 3.3-kb cDNA containing the 552-bp region encoding the human DRM protein. CKTFS1B1 was localized on human chromosome 15q13→ q15 by somatic cell hybrid analysis and, more precisely, using radiation hybrids, to a region of markers linked to SGNE1, secretory granule neuroendocrine protein 1 and RYR3, the ryanodyne receptor 3. Northern blot analysis showed the presence of a single DRM-specific mRNA expressed in different human tissues, including brain, ovary, intestine and colon. In the brain, DRM expression is associated with the region localized around the internal capsule in the large subcortical nuclei. DRM appears to be predominantly expressed in normal cells and tissues, including normal neurons, astrocytes and fibroblasts. Interestingly, we detected DRM expression in normal cells obtained from several patients, but not in tumor cell lines established from the same patients. The data suggest that down-regulation of DRM is associated with tumor progression, and support the hypothesis that human DRM may play an important role during both neuroembryological development and carcinogenesis. </description><subject>Aging - genetics</subject><subject>Aging - metabolism</subject><subject>Biological and medical sciences</subject><subject>Bone Morphogenetic Proteins - metabolism</subject><subject>Brain - cytology</subject><subject>Brain - embryology</subject><subject>Brain - growth & development</subject><subject>Brain - metabolism</subject><subject>Cell Line</subject><subject>chromosome 15</subject><subject>Chromosomes, Human, Pair 15 - genetics</subject><subject>CKTSF1B1 gene</subject><subject>Cloning, Molecular</subject><subject>DRM/GREMLIN protein</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Profiling</subject><subject>Genes. Genome</subject><subject>Genetic Linkage - genetics</subject><subject>Humans</subject><subject>Hybrid Cells</subject><subject>Intercellular Signaling Peptides and Proteins</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Neoplasms - genetics</subject><subject>Neoplasms - metabolism</subject><subject>Neoplasms - pathology</subject><subject>Physical Chromosome Mapping</subject><subject>Proteins - genetics</subject><subject>Proteins - metabolism</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - genetics</subject><subject>Tumor Cells, Cultured</subject><issn>1424-8581</issn><issn>0301-0171</issn><issn>1424-859X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpt0FtLwzAUB_Agipfpg8-CBBHRh7meXNr0UatOcVOYE3wraZq6am8mLbhvb3RDRcxDEnJ-nCR_hHbBOwXg4cBzAzj3xQraBEZYX_DwafV7L2ADbVn74pBg3F9HG-CJkIWMbCJ1MRkPhpPL8ejmDh9Ht9OHKziHE1zKxuK2xrOulBVWM1OXta1LjYFjWaU4t3iWP8-KOdbvjdHWandWYZl2RfsFMt3KAidG5tU2WstkYfXOcu2hx6vLaXTdH90Pb6KzUV8x4G0fgCaBxwIhOFdBKIROBUu4SJLEF9zXiioCJPRBKe3-EaQqk5IolbFM8FRz2kNHi76Nqd86bdu4zK3SRSErXXc2hiCgNPCpgwd_4Evdmcq9LSaEEUrd7NDJAilTW2t0FjcmL6WZx-DFn7HH37E7u79s2CWlTn_JRc4OHC6BtEoWmZGVyu2PY4RwETi2t2Cv0jxr81Nf3nLwbzWKhl8gbtKMfgDkn5ph</recordid><startdate>20000101</startdate><enddate>20000101</enddate><creator>Topol, L.Z.</creator><creator>Modi, W.S.</creator><creator>Koochekpour, S.</creator><creator>Blair, D.G.</creator><general>Karger</general><general>S. 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Psychology</topic><topic>Gene Expression Profiling</topic><topic>Genes. Genome</topic><topic>Genetic Linkage - genetics</topic><topic>Humans</topic><topic>Hybrid Cells</topic><topic>Intercellular Signaling Peptides and Proteins</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Neoplasms - genetics</topic><topic>Neoplasms - metabolism</topic><topic>Neoplasms - pathology</topic><topic>Physical Chromosome Mapping</topic><topic>Proteins - genetics</topic><topic>Proteins - metabolism</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - genetics</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Topol, L.Z.</creatorcontrib><creatorcontrib>Modi, W.S.</creatorcontrib><creatorcontrib>Koochekpour, S.</creatorcontrib><creatorcontrib>Blair, D.G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Science Journals</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>SIRS Editorial</collection><jtitle>Cytogenetic and genome research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Topol, L.Z.</au><au>Modi, W.S.</au><au>Koochekpour, S.</au><au>Blair, D.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DRM/GREMLIN (CKTSF1B1) maps to human chromosome 15 and is highly expressed in adult and fetal brain</atitle><jtitle>Cytogenetic and genome research</jtitle><addtitle>Cytogenet Genome Res</addtitle><date>2000-01-01</date><risdate>2000</risdate><volume>89</volume><issue>1-2</issue><spage>79</spage><epage>84</epage><pages>79-84</pages><issn>1424-8581</issn><issn>0301-0171</issn><eissn>1424-859X</eissn><coden>CGCGBR</coden><abstract>Abstract. We have mapped and characterized the human homolog of Drm/Gremlin (CKTFS1B1), a member of a family of BMP antagonists that have been linked to both developmental and transformation-related functions. By screening a human cDNA library, we isolated a 3.3-kb cDNA containing the 552-bp region encoding the human DRM protein. CKTFS1B1 was localized on human chromosome 15q13→ q15 by somatic cell hybrid analysis and, more precisely, using radiation hybrids, to a region of markers linked to SGNE1, secretory granule neuroendocrine protein 1 and RYR3, the ryanodyne receptor 3. Northern blot analysis showed the presence of a single DRM-specific mRNA expressed in different human tissues, including brain, ovary, intestine and colon. In the brain, DRM expression is associated with the region localized around the internal capsule in the large subcortical nuclei. DRM appears to be predominantly expressed in normal cells and tissues, including normal neurons, astrocytes and fibroblasts. Interestingly, we detected DRM expression in normal cells obtained from several patients, but not in tumor cell lines established from the same patients. The data suggest that down-regulation of DRM is associated with tumor progression, and support the hypothesis that human DRM may play an important role during both neuroembryological development and carcinogenesis. </abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>10894942</pmid><doi>10.1159/000015568</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1424-8581 |
| ispartof | Cytogenetic and genome research, 2000-01, Vol.89 (1-2), p.79-84 |
| issn | 1424-8581 0301-0171 1424-859X |
| language | eng |
| recordid | cdi_pubmed_primary_10894942 |
| source | Karger Journals; MEDLINE |
| subjects | Aging - genetics Aging - metabolism Biological and medical sciences Bone Morphogenetic Proteins - metabolism Brain - cytology Brain - embryology Brain - growth & development Brain - metabolism Cell Line chromosome 15 Chromosomes, Human, Pair 15 - genetics CKTSF1B1 gene Cloning, Molecular DRM/GREMLIN protein Fundamental and applied biological sciences. Psychology Gene Expression Profiling Genes. Genome Genetic Linkage - genetics Humans Hybrid Cells Intercellular Signaling Peptides and Proteins Molecular and cellular biology Molecular genetics Molecular Sequence Data Neoplasms - genetics Neoplasms - metabolism Neoplasms - pathology Physical Chromosome Mapping Proteins - genetics Proteins - metabolism Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism RNA, Messenger - analysis RNA, Messenger - genetics Tumor Cells, Cultured |
| title | DRM/GREMLIN (CKTSF1B1) maps to human chromosome 15 and is highly expressed in adult and fetal brain |
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