Synaptophysin Regulates Clathrin-Independent Endocytosis of Synaptic Vesicles
The GTPase dynamin I is required for synaptic vesicle (SV) endocytosis. Our observation that dynamin binds to the SV protein synaptophysin in a Ca2+-dependent fashion suggested the possibility that a dynamin/synaptophysin complex functions in SV recycling. In this paper we show that disruption of th...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2000-05, Vol.97 (11), p.6120-6125 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Daly, Christopher Sugimori, Mutsuyuki Moreira, Jorge E. Ziff, Edward B. Llinas, Rodolfo |
| description | The GTPase dynamin I is required for synaptic vesicle (SV) endocytosis. Our observation that dynamin binds to the SV protein synaptophysin in a Ca2+-dependent fashion suggested the possibility that a dynamin/synaptophysin complex functions in SV recycling. In this paper we show that disruption of the dynamin/synaptophysin interaction by peptide injection into the squid giant synapse preterminal results in a decrease in transmitter release during high-frequency stimulation, indicating an inhibition of SV recycling. Electron microscopy of these synapses reveals a depletion of SVs, demonstrating a block of vesicle retrieval after fusion. In addition, we observed an increase in clathrin-coated vesicles, indicating that the peptide does not block clathrin-dependent endocytosis. We conclude that the dynamin/synaptophysin complex functions in a clathrin-independent mechanism of SV endocytosis that is required for efficient synaptic transmission. |
| doi_str_mv | 10.1073/pnas.97.11.6120 |
| format | Article |
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Our observation that dynamin binds to the SV protein synaptophysin in a Ca2+-dependent fashion suggested the possibility that a dynamin/synaptophysin complex functions in SV recycling. In this paper we show that disruption of the dynamin/synaptophysin interaction by peptide injection into the squid giant synapse preterminal results in a decrease in transmitter release during high-frequency stimulation, indicating an inhibition of SV recycling. Electron microscopy of these synapses reveals a depletion of SVs, demonstrating a block of vesicle retrieval after fusion. In addition, we observed an increase in clathrin-coated vesicles, indicating that the peptide does not block clathrin-dependent endocytosis. 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Our observation that dynamin binds to the SV protein synaptophysin in a Ca2+-dependent fashion suggested the possibility that a dynamin/synaptophysin complex functions in SV recycling. In this paper we show that disruption of the dynamin/synaptophysin interaction by peptide injection into the squid giant synapse preterminal results in a decrease in transmitter release during high-frequency stimulation, indicating an inhibition of SV recycling. Electron microscopy of these synapses reveals a depletion of SVs, demonstrating a block of vesicle retrieval after fusion. In addition, we observed an increase in clathrin-coated vesicles, indicating that the peptide does not block clathrin-dependent endocytosis. We conclude that the dynamin/synaptophysin complex functions in a clathrin-independent mechanism of SV endocytosis that is required for efficient synaptic transmission.</description><subject>Action potentials</subject><subject>Animals</subject><subject>Biological Sciences</subject><subject>Calcium - physiology</subject><subject>Cell membranes</subject><subject>Clathrin - physiology</subject><subject>Decapodiformes</subject><subject>dynamin</subject><subject>Dynamin I</subject><subject>Dynamins</subject><subject>Endocytosis</subject><subject>Endocytosis - physiology</subject><subject>GTP Phosphohydrolases - physiology</subject><subject>Macromolecular Substances</subject><subject>Membrane Fusion</subject><subject>Nerve Tissue Proteins - physiology</subject><subject>Neurology</subject><subject>Neuroscience</subject><subject>Peptides</subject><subject>Proteins</subject><subject>Radio transmitters</subject><subject>Recombinant Fusion Proteins - physiology</subject><subject>Recycling</subject><subject>Squid</subject><subject>Stellate Ganglion - cytology</subject><subject>Synapses</subject><subject>Synaptic Transmission - physiology</subject><subject>Synaptic Vesicles - physiology</subject><subject>Synaptophysin - physiology</subject><subject>Transmitters</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS1ERZfCmQMSijjAKdsZxx-xxAWtCq1UhMTX1XIcp5tV1k7jBLH_PV5labcIicvMYX7vzdiPkBcISwRZnPfexKWSS8SlQAqPyAJBYS6YgsdkAUBlXjLKTsnTGDcAoHgJT8gpQkkLxfmCfPq686YfQ7_exdZnX9zN1JnRxWyV2npofX7la9e7VPyYXfg62N0YYhuz0GSztrXZDxdb27n4jJw0povu-aGfke8fLr6tLvPrzx-vVu-vc8tLMeZOlaJpjC0EYyBcwepCAW24pVxZyTnUVdWImqITrALOaC2ooOCcZKZiihdn5N3s20_V1tU23TaYTvdDuzXDTgfT6ocT3671TfipseSiTPI3B_kQbicXR71to3VdZ7wLU9QSkfJCqv-CKDmVrMQEvv4L3IRp8OkPNAUsEqZEgs5nyA4hxsE1dwcj6H2ceh-nVlIj6n2cSfHq-J1H_JxfAt4egL3yz_jeQTdT143u13hk9W8yAS9nYBPHMNyvoikULH4DioG92A</recordid><startdate>20000523</startdate><enddate>20000523</enddate><creator>Daly, Christopher</creator><creator>Sugimori, Mutsuyuki</creator><creator>Moreira, Jorge E.</creator><creator>Ziff, Edward B.</creator><creator>Llinas, Rodolfo</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><general>The National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20000523</creationdate><title>Synaptophysin Regulates Clathrin-Independent Endocytosis of Synaptic Vesicles</title><author>Daly, Christopher ; 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| subjects | Action potentials Animals Biological Sciences Calcium - physiology Cell membranes Clathrin - physiology Decapodiformes dynamin Dynamin I Dynamins Endocytosis Endocytosis - physiology GTP Phosphohydrolases - physiology Macromolecular Substances Membrane Fusion Nerve Tissue Proteins - physiology Neurology Neuroscience Peptides Proteins Radio transmitters Recombinant Fusion Proteins - physiology Recycling Squid Stellate Ganglion - cytology Synapses Synaptic Transmission - physiology Synaptic Vesicles - physiology Synaptophysin - physiology Transmitters |
| title | Synaptophysin Regulates Clathrin-Independent Endocytosis of Synaptic Vesicles |
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