Mechanical properties of the tracheal mucosal membrane in the rabbit. II. Morphometric analysis
1 Department of Chemical and Bio-Resource Engineering, University of British Columbia, Vancouver V6T 1Z4; and 2 Pulmonary Research Laboratory, St. Paul's Hospital, University of British Columbia, Vancouver, British Columbia, Canada V6Z 1Y6 Folding of the airway mucosal membrane provides a mec...
Gespeichert in:
| Veröffentlicht in: | Journal of applied physiology (1985) 2000-03, Vol.88 (3), p.1022-1028 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1028 |
|---|---|
| container_issue | 3 |
| container_start_page | 1022 |
| container_title | Journal of applied physiology (1985) |
| container_volume | 88 |
| creator | Wang, Lu Pinder, Kenneth L Bert, Joel L Okazawa, Mitsushi Pare, Peter D |
| description | 1 Department of Chemical and Bio-Resource
Engineering, University of British Columbia, Vancouver V6T 1Z4; and
2 Pulmonary Research Laboratory, St. Paul's
Hospital, University of British Columbia, Vancouver, British
Columbia, Canada V6Z 1Y6
Folding of the airway
mucosal membrane provides a mechanical load that impedes airway smooth
muscle contraction. Mechanical testing of rabbit tracheal mucosal
membrane showed that the membrane is stiffer in the longitudinal than
in the circumferential direction of the airway. To explain this
difference in the mechanical properties, we studied the morphological
structure of the rabbit tracheal mucosal membrane in both longitudinal
and circumferential directions. The collagen fibers were found to form
a random meshwork, which would not account for differences in stiffness
in the longitudinal and circumferential directions. The volume fraction
of the elastic fibers was measured using a point-counting technique.
The orientation of the elastic fibers in the tissue samples was
measured using a new method based on simple geometry and probability.
The results showed that the volume fraction of the elastic fibers in
the rabbit tracheal mucosal membrane was ~5% and that the elastic
fibers were mainly oriented in the longitudinal direction. Age had no statistically significant effect on either the volume fraction or the
orientation of the elastic fibers. Linear correlations were found
between the steady-state stiffness and the quantity of the elastic
fibers oriented in the direction of testing.
elastin; collagen; imaging analysis |
| doi_str_mv | 10.1152/jappl.2000.88.3.1022 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmed_primary_10710399</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70953018</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-46f3750d601cf878031124beab5b57b9e032fca9ec35596f52f85338b9d9dd823</originalsourceid><addsrcrecordid>eNp1kE1v1DAURS0EokPhHyAUsUDdJPgjHjtLVNEyUis2ZW3ZznPjkTMOdqIy_x6nU0GFxMqLd-_R9UHoPcENIZx-3utpCg3FGDdSNqwhmNIXaFNOtCZbTF6ijRQc14JLcYbe5LzHmLQtJ6_RGcGCYNZ1G6RuwQ764K0O1ZTiBGn2kKvoqnmAak7aDlBO42JjXl8YTdIHqPzhMZC0MX5uqt2uqW5jmoY4wpy8rfRBh2P2-S165XTI8O7pPUc_rr7eXX6rb75f7y6_3NS2Fe1ct1vHyti-7LZOCokZIbQ1oA03XJgOMKPO6g4s47zbOk6d5IxJ0_Vd30vKztGnE7d84ucCeVajzxZCKGPjkpXAHWeYyBL8-E9wH5dU1mZFKSUdp2KltaeQTTHnBE5NyY86HRXBarWvHu2r1b6SUjG12i-1D0_sxYzQPyuddP_lDv5-ePAJ1DQUSzHE-6O6WkK4g1_zyv7DVFPvSu3i_7WSfjbhNwl3okU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>222195272</pqid></control><display><type>article</type><title>Mechanical properties of the tracheal mucosal membrane in the rabbit. II. Morphometric analysis</title><source>MEDLINE</source><source>American Physiological Society</source><source>Alma/SFX Local Collection</source><source>EZB Electronic Journals Library</source><creator>Wang, Lu ; Pinder, Kenneth L ; Bert, Joel L ; Okazawa, Mitsushi ; Pare, Peter D</creator><creatorcontrib>Wang, Lu ; Pinder, Kenneth L ; Bert, Joel L ; Okazawa, Mitsushi ; Pare, Peter D</creatorcontrib><description>1 Department of Chemical and Bio-Resource
Engineering, University of British Columbia, Vancouver V6T 1Z4; and
2 Pulmonary Research Laboratory, St. Paul's
Hospital, University of British Columbia, Vancouver, British
Columbia, Canada V6Z 1Y6
Folding of the airway
mucosal membrane provides a mechanical load that impedes airway smooth
muscle contraction. Mechanical testing of rabbit tracheal mucosal
membrane showed that the membrane is stiffer in the longitudinal than
in the circumferential direction of the airway. To explain this
difference in the mechanical properties, we studied the morphological
structure of the rabbit tracheal mucosal membrane in both longitudinal
and circumferential directions. The collagen fibers were found to form
a random meshwork, which would not account for differences in stiffness
in the longitudinal and circumferential directions. The volume fraction
of the elastic fibers was measured using a point-counting technique.
The orientation of the elastic fibers in the tissue samples was
measured using a new method based on simple geometry and probability.
The results showed that the volume fraction of the elastic fibers in
the rabbit tracheal mucosal membrane was ~5% and that the elastic
fibers were mainly oriented in the longitudinal direction. Age had no statistically significant effect on either the volume fraction or the
orientation of the elastic fibers. Linear correlations were found
between the steady-state stiffness and the quantity of the elastic
fibers oriented in the direction of testing.
elastin; collagen; imaging analysis</description><identifier>ISSN: 8750-7587</identifier><identifier>EISSN: 1522-1601</identifier><identifier>DOI: 10.1152/jappl.2000.88.3.1022</identifier><identifier>PMID: 10710399</identifier><language>eng</language><publisher>United States: Am Physiological Soc</publisher><subject>Animals ; Biomechanical Phenomena ; Biometry ; Elastic Tissue - anatomy & histology ; Elastic Tissue - physiology ; Female ; In Vitro Techniques ; Microscopy, Confocal ; Rabbits ; Respiratory Mechanics - physiology ; Respiratory Mucosa - anatomy & histology ; Respiratory Mucosa - physiology ; Space life sciences ; Trachea - anatomy & histology ; Trachea - physiology</subject><ispartof>Journal of applied physiology (1985), 2000-03, Vol.88 (3), p.1022-1028</ispartof><rights>Copyright American Physiological Society Mar 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-46f3750d601cf878031124beab5b57b9e032fca9ec35596f52f85338b9d9dd823</citedby><cites>FETCH-LOGICAL-c474t-46f3750d601cf878031124beab5b57b9e032fca9ec35596f52f85338b9d9dd823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3026,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10710399$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Lu</creatorcontrib><creatorcontrib>Pinder, Kenneth L</creatorcontrib><creatorcontrib>Bert, Joel L</creatorcontrib><creatorcontrib>Okazawa, Mitsushi</creatorcontrib><creatorcontrib>Pare, Peter D</creatorcontrib><title>Mechanical properties of the tracheal mucosal membrane in the rabbit. II. Morphometric analysis</title><title>Journal of applied physiology (1985)</title><addtitle>J Appl Physiol (1985)</addtitle><description>1 Department of Chemical and Bio-Resource
Engineering, University of British Columbia, Vancouver V6T 1Z4; and
2 Pulmonary Research Laboratory, St. Paul's
Hospital, University of British Columbia, Vancouver, British
Columbia, Canada V6Z 1Y6
Folding of the airway
mucosal membrane provides a mechanical load that impedes airway smooth
muscle contraction. Mechanical testing of rabbit tracheal mucosal
membrane showed that the membrane is stiffer in the longitudinal than
in the circumferential direction of the airway. To explain this
difference in the mechanical properties, we studied the morphological
structure of the rabbit tracheal mucosal membrane in both longitudinal
and circumferential directions. The collagen fibers were found to form
a random meshwork, which would not account for differences in stiffness
in the longitudinal and circumferential directions. The volume fraction
of the elastic fibers was measured using a point-counting technique.
The orientation of the elastic fibers in the tissue samples was
measured using a new method based on simple geometry and probability.
The results showed that the volume fraction of the elastic fibers in
the rabbit tracheal mucosal membrane was ~5% and that the elastic
fibers were mainly oriented in the longitudinal direction. Age had no statistically significant effect on either the volume fraction or the
orientation of the elastic fibers. Linear correlations were found
between the steady-state stiffness and the quantity of the elastic
fibers oriented in the direction of testing.
elastin; collagen; imaging analysis</description><subject>Animals</subject><subject>Biomechanical Phenomena</subject><subject>Biometry</subject><subject>Elastic Tissue - anatomy & histology</subject><subject>Elastic Tissue - physiology</subject><subject>Female</subject><subject>In Vitro Techniques</subject><subject>Microscopy, Confocal</subject><subject>Rabbits</subject><subject>Respiratory Mechanics - physiology</subject><subject>Respiratory Mucosa - anatomy & histology</subject><subject>Respiratory Mucosa - physiology</subject><subject>Space life sciences</subject><subject>Trachea - anatomy & histology</subject><subject>Trachea - physiology</subject><issn>8750-7587</issn><issn>1522-1601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1v1DAURS0EokPhHyAUsUDdJPgjHjtLVNEyUis2ZW3ZznPjkTMOdqIy_x6nU0GFxMqLd-_R9UHoPcENIZx-3utpCg3FGDdSNqwhmNIXaFNOtCZbTF6ijRQc14JLcYbe5LzHmLQtJ6_RGcGCYNZ1G6RuwQ764K0O1ZTiBGn2kKvoqnmAak7aDlBO42JjXl8YTdIHqPzhMZC0MX5uqt2uqW5jmoY4wpy8rfRBh2P2-S165XTI8O7pPUc_rr7eXX6rb75f7y6_3NS2Fe1ct1vHyti-7LZOCokZIbQ1oA03XJgOMKPO6g4s47zbOk6d5IxJ0_Vd30vKztGnE7d84ucCeVajzxZCKGPjkpXAHWeYyBL8-E9wH5dU1mZFKSUdp2KltaeQTTHnBE5NyY86HRXBarWvHu2r1b6SUjG12i-1D0_sxYzQPyuddP_lDv5-ePAJ1DQUSzHE-6O6WkK4g1_zyv7DVFPvSu3i_7WSfjbhNwl3okU</recordid><startdate>20000301</startdate><enddate>20000301</enddate><creator>Wang, Lu</creator><creator>Pinder, Kenneth L</creator><creator>Bert, Joel L</creator><creator>Okazawa, Mitsushi</creator><creator>Pare, Peter D</creator><general>Am Physiological Soc</general><general>American Physiological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TS</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20000301</creationdate><title>Mechanical properties of the tracheal mucosal membrane in the rabbit. II. Morphometric analysis</title><author>Wang, Lu ; Pinder, Kenneth L ; Bert, Joel L ; Okazawa, Mitsushi ; Pare, Peter D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-46f3750d601cf878031124beab5b57b9e032fca9ec35596f52f85338b9d9dd823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biomechanical Phenomena</topic><topic>Biometry</topic><topic>Elastic Tissue - anatomy & histology</topic><topic>Elastic Tissue - physiology</topic><topic>Female</topic><topic>In Vitro Techniques</topic><topic>Microscopy, Confocal</topic><topic>Rabbits</topic><topic>Respiratory Mechanics - physiology</topic><topic>Respiratory Mucosa - anatomy & histology</topic><topic>Respiratory Mucosa - physiology</topic><topic>Space life sciences</topic><topic>Trachea - anatomy & histology</topic><topic>Trachea - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Lu</creatorcontrib><creatorcontrib>Pinder, Kenneth L</creatorcontrib><creatorcontrib>Bert, Joel L</creatorcontrib><creatorcontrib>Okazawa, Mitsushi</creatorcontrib><creatorcontrib>Pare, Peter D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied physiology (1985)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Lu</au><au>Pinder, Kenneth L</au><au>Bert, Joel L</au><au>Okazawa, Mitsushi</au><au>Pare, Peter D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanical properties of the tracheal mucosal membrane in the rabbit. II. Morphometric analysis</atitle><jtitle>Journal of applied physiology (1985)</jtitle><addtitle>J Appl Physiol (1985)</addtitle><date>2000-03-01</date><risdate>2000</risdate><volume>88</volume><issue>3</issue><spage>1022</spage><epage>1028</epage><pages>1022-1028</pages><issn>8750-7587</issn><eissn>1522-1601</eissn><abstract>1 Department of Chemical and Bio-Resource
Engineering, University of British Columbia, Vancouver V6T 1Z4; and
2 Pulmonary Research Laboratory, St. Paul's
Hospital, University of British Columbia, Vancouver, British
Columbia, Canada V6Z 1Y6
Folding of the airway
mucosal membrane provides a mechanical load that impedes airway smooth
muscle contraction. Mechanical testing of rabbit tracheal mucosal
membrane showed that the membrane is stiffer in the longitudinal than
in the circumferential direction of the airway. To explain this
difference in the mechanical properties, we studied the morphological
structure of the rabbit tracheal mucosal membrane in both longitudinal
and circumferential directions. The collagen fibers were found to form
a random meshwork, which would not account for differences in stiffness
in the longitudinal and circumferential directions. The volume fraction
of the elastic fibers was measured using a point-counting technique.
The orientation of the elastic fibers in the tissue samples was
measured using a new method based on simple geometry and probability.
The results showed that the volume fraction of the elastic fibers in
the rabbit tracheal mucosal membrane was ~5% and that the elastic
fibers were mainly oriented in the longitudinal direction. Age had no statistically significant effect on either the volume fraction or the
orientation of the elastic fibers. Linear correlations were found
between the steady-state stiffness and the quantity of the elastic
fibers oriented in the direction of testing.
elastin; collagen; imaging analysis</abstract><cop>United States</cop><pub>Am Physiological Soc</pub><pmid>10710399</pmid><doi>10.1152/jappl.2000.88.3.1022</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 8750-7587 |
| ispartof | Journal of applied physiology (1985), 2000-03, Vol.88 (3), p.1022-1028 |
| issn | 8750-7587 1522-1601 |
| language | eng |
| recordid | cdi_pubmed_primary_10710399 |
| source | MEDLINE; American Physiological Society; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Animals Biomechanical Phenomena Biometry Elastic Tissue - anatomy & histology Elastic Tissue - physiology Female In Vitro Techniques Microscopy, Confocal Rabbits Respiratory Mechanics - physiology Respiratory Mucosa - anatomy & histology Respiratory Mucosa - physiology Space life sciences Trachea - anatomy & histology Trachea - physiology |
| title | Mechanical properties of the tracheal mucosal membrane in the rabbit. II. Morphometric analysis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T13%3A43%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mechanical%20properties%20of%20the%20tracheal%20mucosal%20membrane%20in%20the%20rabbit.%20II.%20Morphometric%20analysis&rft.jtitle=Journal%20of%20applied%20physiology%20(1985)&rft.au=Wang,%20Lu&rft.date=2000-03-01&rft.volume=88&rft.issue=3&rft.spage=1022&rft.epage=1028&rft.pages=1022-1028&rft.issn=8750-7587&rft.eissn=1522-1601&rft_id=info:doi/10.1152/jappl.2000.88.3.1022&rft_dat=%3Cproquest_pubme%3E70953018%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=222195272&rft_id=info:pmid/10710399&rfr_iscdi=true |