Purification of a 28 kDa Babesia (Theileria) equi antigen and a 29 kDa spurious erythrocyte antigen from in vitro culture through ion exchange chromatography

An extract of in vitro cultivated Babesia equi was fractionated using a MonoQ anion exchange column. Separation of a 28 kDa B. equi antigen from a 29 kDa spurious erythrocyte antigen, both of which were intensely immunoreactive, was achieved by chromatography of the infected erythrocyte proteins. Us...

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Veröffentlicht in:	Veterinary parasitology 1999-09, Vol.86 (1), p.63-70
Hauptverfasser:	Samuel, Temesgen, Böse, Reinhard, Schelp, Christian
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Anion exchange chromatography ANTIGENE ANTIGENOS ANTIGENS Antigens, Protozoan - blood Antigens, Protozoan - immunology Antigens, Protozoan - isolation & purification Babesia (Theileria) equi Babesia - immunology BABESIA EQUI Babesiosis - diagnosis Babesiosis - immunology Blotting, Western - veterinary CHROMATOGRAPHIE D'ECHANGE IONIQUE Chromatography, Ion Exchange - veterinary CROMATOGRAFIA DE CAMBIO IONICO Electrophoresis, Polyacrylamide Gel - veterinary Erythrocytes - immunology Erythrocytes - parasitology Glycine-SDS-PAGE Horses ION EXCHANGE CHROMATOGRAPHY Isoelectric Focusing - veterinary Sensitivity and Specificity Tricine-SDS-PAGE
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