Store‐Operated Ca2+ Influx and Voltage‐Gated Ca2+ Channels Coupled to Exocytosis in Pheochromocytoma (PC12) Cells
: Microamperometry was used to monitor quantal catecholamine release from individual PC12 cells in response to raised extracellular K+ and caffeine. K+‐evoked exocytosis was entirely dependent on Ca2+ influx through voltage‐gated Ca2+ channels, and of the subtypes of such channels present in these c...
Gespeichert in:
| Veröffentlicht in: | Journal of neurochemistry 1999-08, Vol.73 (2), p.874-880 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 880 |
|---|---|
| container_issue | 2 |
| container_start_page | 874 |
| container_title | Journal of neurochemistry |
| container_volume | 73 |
| creator | Taylor, S. C. Peers, C. |
| description | : Microamperometry was used to monitor quantal
catecholamine release from individual PC12 cells in response to raised
extracellular K+ and caffeine. K+‐evoked exocytosis was
entirely dependent on Ca2+ influx through voltage‐gated
Ca2+ channels, and of the subtypes of such channels present in
these cells, influx through N‐type was primarily responsible for triggering
exocytosis. L‐type channels played a minor role in mediating
K+‐evoked secretion, whereas P/Q‐type channels did not appear to be
involved in secretion at all. Caffeine also evoked catecholamine release from
PC12 cells, but only in the presence of extracellular Ca2+.
Application of caffeine in Ca2+‐free solutions evoked large,
transient rises of [Ca2+]i, but did not trigger
exocytosis. When Ca2+ was restored to the extracellular solution
(in the absence of caffeine), store‐operated Ca2+ influx was
observed, which evoked exocytosis. The amount of secretion evoked by this
influx pathway was far greater than release triggered by influx through L‐type
Ca2+ channels, but less than that caused by Ca2+ influx
through N‐type channels. Our results indicate that exocytosis may be regulated
even in excitable cells by Ca2+ influx through pathways other than
voltage‐gated Ca2+ channels. |
| doi_str_mv | 10.1046/j.1471-4159.1999.0730874.x |
| format | Article |
| fullrecord | <record><control><sourceid>wiley_pubme</sourceid><recordid>TN_cdi_pubmed_primary_10428087</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>JNC0730874</sourcerecordid><originalsourceid>FETCH-LOGICAL-j3454-37d19b38a74c09632ecc8dbdda374b6628ad7199b094bb7d87310e1af4d6951f3</originalsourceid><addsrcrecordid>eNpNkU1OwzAQhS0EoqVwBWQhFiCUYMduHC8hKqWoopX42VpO7NBEblLFqUh3HIEzchISGiir0cz75knzBoAzjFyMqH-duZgy7FA85C7mnLuIERQw6tZ7oP8n7YM-Qp7nEES9HjiyNkMI-9THh6DX2HhBs9IH66eqKPXXx-dspUtZaQVD6V3BSZ6YdQ1lruBrYSr51iLjnR4uZJ5rY2FYrFemmVYFHNVFvKkKm1qY5nC-0EW8KIvlz3Ap4cU8xN4lDLUx9hgcJNJYfdLVAXi5Gz2H9850Np6EN1MnI3RIHcIU5hEJJKMx4j7xdBwHKlJKEkYj3_cCqViTQIQ4jSKmAkYw0lgmVPl8iBMyAKdb39U6WmolVmW6lOVG_N7fAOcdIG0sTVLKPE7tjuMENYk32O0We0-N3vyzaZ18kYk2ddGmLtqHiO4hohYPj2HXkG8ht3_g</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Store‐Operated Ca2+ Influx and Voltage‐Gated Ca2+ Channels Coupled to Exocytosis in Pheochromocytoma (PC12) Cells</title><source>MEDLINE</source><source>Wiley Online Library Free Content</source><source>Access via Wiley Online Library</source><source>IngentaConnect Free/Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Free Full-Text Journals in Chemistry</source><creator>Taylor, S. C. ; Peers, C.</creator><creatorcontrib>Taylor, S. C. ; Peers, C.</creatorcontrib><description>: Microamperometry was used to monitor quantal
catecholamine release from individual PC12 cells in response to raised
extracellular K+ and caffeine. K+‐evoked exocytosis was
entirely dependent on Ca2+ influx through voltage‐gated
Ca2+ channels, and of the subtypes of such channels present in
these cells, influx through N‐type was primarily responsible for triggering
exocytosis. L‐type channels played a minor role in mediating
K+‐evoked secretion, whereas P/Q‐type channels did not appear to be
involved in secretion at all. Caffeine also evoked catecholamine release from
PC12 cells, but only in the presence of extracellular Ca2+.
Application of caffeine in Ca2+‐free solutions evoked large,
transient rises of [Ca2+]i, but did not trigger
exocytosis. When Ca2+ was restored to the extracellular solution
(in the absence of caffeine), store‐operated Ca2+ influx was
observed, which evoked exocytosis. The amount of secretion evoked by this
influx pathway was far greater than release triggered by influx through L‐type
Ca2+ channels, but less than that caused by Ca2+ influx
through N‐type channels. Our results indicate that exocytosis may be regulated
even in excitable cells by Ca2+ influx through pathways other than
voltage‐gated Ca2+ channels.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1046/j.1471-4159.1999.0730874.x</identifier><identifier>PMID: 10428087</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford UK: Blackwell Science Ltd</publisher><subject>Animals ; Biological and medical sciences ; Biological Transport - drug effects ; Biological Transport - physiology ; Ca2+ channels ; Ca2+ stores ; Caffeine - pharmacology ; Calcium - metabolism ; Calcium Channel Blockers - pharmacology ; Calcium Channels - metabolism ; Calcium Channels, L-Type ; Calcium Channels, N-Type ; Catecholamines ; Catecholamines - metabolism ; Cell physiology ; Central Nervous System Stimulants - pharmacology ; Electrophysiology ; Exocytosis ; Exocytosis - physiology ; Fundamental and applied biological sciences. Psychology ; Molecular and cellular biology ; Neurons - chemistry ; Neurons - cytology ; Neurons - metabolism ; Nifedipine - pharmacology ; omega-Agatoxin IVA ; omega-Conotoxin GVIA ; PC12 Cells ; Peptides - pharmacology ; Potassium - pharmacology ; Rats ; Secretion. Exocytosis ; Spider Venoms - pharmacology</subject><ispartof>Journal of neurochemistry, 1999-08, Vol.73 (2), p.874-880</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1471-4159.1999.0730874.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1471-4159.1999.0730874.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1930046$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10428087$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Taylor, S. C.</creatorcontrib><creatorcontrib>Peers, C.</creatorcontrib><title>Store‐Operated Ca2+ Influx and Voltage‐Gated Ca2+ Channels Coupled to Exocytosis in Pheochromocytoma (PC12) Cells</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>: Microamperometry was used to monitor quantal
catecholamine release from individual PC12 cells in response to raised
extracellular K+ and caffeine. K+‐evoked exocytosis was
entirely dependent on Ca2+ influx through voltage‐gated
Ca2+ channels, and of the subtypes of such channels present in
these cells, influx through N‐type was primarily responsible for triggering
exocytosis. L‐type channels played a minor role in mediating
K+‐evoked secretion, whereas P/Q‐type channels did not appear to be
involved in secretion at all. Caffeine also evoked catecholamine release from
PC12 cells, but only in the presence of extracellular Ca2+.
Application of caffeine in Ca2+‐free solutions evoked large,
transient rises of [Ca2+]i, but did not trigger
exocytosis. When Ca2+ was restored to the extracellular solution
(in the absence of caffeine), store‐operated Ca2+ influx was
observed, which evoked exocytosis. The amount of secretion evoked by this
influx pathway was far greater than release triggered by influx through L‐type
Ca2+ channels, but less than that caused by Ca2+ influx
through N‐type channels. Our results indicate that exocytosis may be regulated
even in excitable cells by Ca2+ influx through pathways other than
voltage‐gated Ca2+ channels.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Transport - drug effects</subject><subject>Biological Transport - physiology</subject><subject>Ca2+ channels</subject><subject>Ca2+ stores</subject><subject>Caffeine - pharmacology</subject><subject>Calcium - metabolism</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Calcium Channels - metabolism</subject><subject>Calcium Channels, L-Type</subject><subject>Calcium Channels, N-Type</subject><subject>Catecholamines</subject><subject>Catecholamines - metabolism</subject><subject>Cell physiology</subject><subject>Central Nervous System Stimulants - pharmacology</subject><subject>Electrophysiology</subject><subject>Exocytosis</subject><subject>Exocytosis - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Molecular and cellular biology</subject><subject>Neurons - chemistry</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Nifedipine - pharmacology</subject><subject>omega-Agatoxin IVA</subject><subject>omega-Conotoxin GVIA</subject><subject>PC12 Cells</subject><subject>Peptides - pharmacology</subject><subject>Potassium - pharmacology</subject><subject>Rats</subject><subject>Secretion. Exocytosis</subject><subject>Spider Venoms - pharmacology</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkU1OwzAQhS0EoqVwBWQhFiCUYMduHC8hKqWoopX42VpO7NBEblLFqUh3HIEzchISGiir0cz75knzBoAzjFyMqH-duZgy7FA85C7mnLuIERQw6tZ7oP8n7YM-Qp7nEES9HjiyNkMI-9THh6DX2HhBs9IH66eqKPXXx-dspUtZaQVD6V3BSZ6YdQ1lruBrYSr51iLjnR4uZJ5rY2FYrFemmVYFHNVFvKkKm1qY5nC-0EW8KIvlz3Ap4cU8xN4lDLUx9hgcJNJYfdLVAXi5Gz2H9850Np6EN1MnI3RIHcIU5hEJJKMx4j7xdBwHKlJKEkYj3_cCqViTQIQ4jSKmAkYw0lgmVPl8iBMyAKdb39U6WmolVmW6lOVG_N7fAOcdIG0sTVLKPE7tjuMENYk32O0We0-N3vyzaZ18kYk2ddGmLtqHiO4hohYPj2HXkG8ht3_g</recordid><startdate>199908</startdate><enddate>199908</enddate><creator>Taylor, S. C.</creator><creator>Peers, C.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>199908</creationdate><title>Store‐Operated Ca2+ Influx and Voltage‐Gated Ca2+ Channels Coupled to Exocytosis in Pheochromocytoma (PC12) Cells</title><author>Taylor, S. C. ; Peers, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j3454-37d19b38a74c09632ecc8dbdda374b6628ad7199b094bb7d87310e1af4d6951f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Transport - drug effects</topic><topic>Biological Transport - physiology</topic><topic>Ca2+ channels</topic><topic>Ca2+ stores</topic><topic>Caffeine - pharmacology</topic><topic>Calcium - metabolism</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Calcium Channels - metabolism</topic><topic>Calcium Channels, L-Type</topic><topic>Calcium Channels, N-Type</topic><topic>Catecholamines</topic><topic>Catecholamines - metabolism</topic><topic>Cell physiology</topic><topic>Central Nervous System Stimulants - pharmacology</topic><topic>Electrophysiology</topic><topic>Exocytosis</topic><topic>Exocytosis - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Molecular and cellular biology</topic><topic>Neurons - chemistry</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Nifedipine - pharmacology</topic><topic>omega-Agatoxin IVA</topic><topic>omega-Conotoxin GVIA</topic><topic>PC12 Cells</topic><topic>Peptides - pharmacology</topic><topic>Potassium - pharmacology</topic><topic>Rats</topic><topic>Secretion. Exocytosis</topic><topic>Spider Venoms - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Taylor, S. C.</creatorcontrib><creatorcontrib>Peers, C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Taylor, S. C.</au><au>Peers, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Store‐Operated Ca2+ Influx and Voltage‐Gated Ca2+ Channels Coupled to Exocytosis in Pheochromocytoma (PC12) Cells</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1999-08</date><risdate>1999</risdate><volume>73</volume><issue>2</issue><spage>874</spage><epage>880</epage><pages>874-880</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>: Microamperometry was used to monitor quantal
catecholamine release from individual PC12 cells in response to raised
extracellular K+ and caffeine. K+‐evoked exocytosis was
entirely dependent on Ca2+ influx through voltage‐gated
Ca2+ channels, and of the subtypes of such channels present in
these cells, influx through N‐type was primarily responsible for triggering
exocytosis. L‐type channels played a minor role in mediating
K+‐evoked secretion, whereas P/Q‐type channels did not appear to be
involved in secretion at all. Caffeine also evoked catecholamine release from
PC12 cells, but only in the presence of extracellular Ca2+.
Application of caffeine in Ca2+‐free solutions evoked large,
transient rises of [Ca2+]i, but did not trigger
exocytosis. When Ca2+ was restored to the extracellular solution
(in the absence of caffeine), store‐operated Ca2+ influx was
observed, which evoked exocytosis. The amount of secretion evoked by this
influx pathway was far greater than release triggered by influx through L‐type
Ca2+ channels, but less than that caused by Ca2+ influx
through N‐type channels. Our results indicate that exocytosis may be regulated
even in excitable cells by Ca2+ influx through pathways other than
voltage‐gated Ca2+ channels.</abstract><cop>Oxford UK</cop><pub>Blackwell Science Ltd</pub><pmid>10428087</pmid><doi>10.1046/j.1471-4159.1999.0730874.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3042 |
| ispartof | Journal of neurochemistry, 1999-08, Vol.73 (2), p.874-880 |
| issn | 0022-3042 1471-4159 |
| language | eng |
| recordid | cdi_pubmed_primary_10428087 |
| source | MEDLINE; Wiley Online Library Free Content; Access via Wiley Online Library; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Animals Biological and medical sciences Biological Transport - drug effects Biological Transport - physiology Ca2+ channels Ca2+ stores Caffeine - pharmacology Calcium - metabolism Calcium Channel Blockers - pharmacology Calcium Channels - metabolism Calcium Channels, L-Type Calcium Channels, N-Type Catecholamines Catecholamines - metabolism Cell physiology Central Nervous System Stimulants - pharmacology Electrophysiology Exocytosis Exocytosis - physiology Fundamental and applied biological sciences. Psychology Molecular and cellular biology Neurons - chemistry Neurons - cytology Neurons - metabolism Nifedipine - pharmacology omega-Agatoxin IVA omega-Conotoxin GVIA PC12 Cells Peptides - pharmacology Potassium - pharmacology Rats Secretion. Exocytosis Spider Venoms - pharmacology |
| title | Store‐Operated Ca2+ Influx and Voltage‐Gated Ca2+ Channels Coupled to Exocytosis in Pheochromocytoma (PC12) Cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T11%3A55%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wiley_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Store%E2%80%90Operated%20Ca2+%20Influx%20and%20Voltage%E2%80%90Gated%20Ca2+%20Channels%20Coupled%20to%20Exocytosis%20in%20Pheochromocytoma%20(PC12)%20Cells&rft.jtitle=Journal%20of%20neurochemistry&rft.au=Taylor,%20S.%20C.&rft.date=1999-08&rft.volume=73&rft.issue=2&rft.spage=874&rft.epage=880&rft.pages=874-880&rft.issn=0022-3042&rft.eissn=1471-4159&rft.coden=JONRA9&rft_id=info:doi/10.1046/j.1471-4159.1999.0730874.x&rft_dat=%3Cwiley_pubme%3EJNC0730874%3C/wiley_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/10428087&rfr_iscdi=true |