Patterning Cells and Their Environments Using Multiple Laminar Fluid Flows in Capillary Networks
This paper describes the use of laminar flow of liquids in capillary systems to pattern the cell culture substrate, to perform patterned cell deposition, and to pattern the cell culture media. We demonstrate the patterning of the cell culture substrate with different proteins, the patterning of diff...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1999-05, Vol.96 (10), p.5545-5548 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Takayama, Shuichi McDonald, J. Cooper Ostuni, Emanuele Liang, Michael N. Paul J. A. Kenis Ismagilov, Rustem F. Whitesides, George M. |
| description | This paper describes the use of laminar flow of liquids in capillary systems to pattern the cell culture substrate, to perform patterned cell deposition, and to pattern the cell culture media. We demonstrate the patterning of the cell culture substrate with different proteins, the patterning of different types of cells adjacent to each other, the patterned delivery of chemicals to adhered cells, and performing enzymatic reactions over select cells or over a portion of a cell. This method offers a way to simultaneously control the characteristics of the surface to which cells are attached, the type of cells that are in their vicinity, and the kind of media that cells or part of a cell are exposed to. The method is experimentally simple, highly adaptable, and requires no special equipment except for an elastomeric relief that can be readily prepared by rapid prototyping. |
| doi_str_mv | 10.1073/pnas.96.10.5545 |
| format | Article |
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Cooper ; Ostuni, Emanuele ; Liang, Michael N. ; Paul J. A. Kenis ; Ismagilov, Rustem F. ; Whitesides, George M.</creator><creatorcontrib>Takayama, Shuichi ; McDonald, J. Cooper ; Ostuni, Emanuele ; Liang, Michael N. ; Paul J. A. Kenis ; Ismagilov, Rustem F. ; Whitesides, George M.</creatorcontrib><description>This paper describes the use of laminar flow of liquids in capillary systems to pattern the cell culture substrate, to perform patterned cell deposition, and to pattern the cell culture media. We demonstrate the patterning of the cell culture substrate with different proteins, the patterning of different types of cells adjacent to each other, the patterned delivery of chemicals to adhered cells, and performing enzymatic reactions over select cells or over a portion of a cell. This method offers a way to simultaneously control the characteristics of the surface to which cells are attached, the type of cells that are in their vicinity, and the kind of media that cells or part of a cell are exposed to. The method is experimentally simple, highly adaptable, and requires no special equipment except for an elastomeric relief that can be readily prepared by rapid prototyping.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.96.10.5545</identifier><identifier>PMID: 10318920</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Biological Sciences ; Cattle ; Cell Adhesion ; Cell biology ; Cell culture techniques ; Cell Culture Techniques - methods ; Cells ; Cells, Cultured ; Cellular biology ; Chemical suspensions ; Culture media ; Culture Media - chemistry ; Endothelium, Vascular - metabolism ; Erythrocytes ; Erythrocytes - metabolism ; Escherichia coli - metabolism ; Flow velocity ; Fluid dynamics ; Fluid flow ; Fluorescence ; Inlets ; Laminar flow ; Liquids ; Physical Sciences ; Proteins - metabolism</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1999-05, Vol.96 (10), p.5545-5548</ispartof><rights>Copyright 1993-1999 The National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences May 11, 1999</rights><rights>Copyright © 1999, The National Academy of Sciences 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-6f12439a63acf93cb7b6a26806bab0e8ddc1330466983e40b11a8ab259c353e53</citedby><cites>FETCH-LOGICAL-c488t-6f12439a63acf93cb7b6a26806bab0e8ddc1330466983e40b11a8ab259c353e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/96/10.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/48153$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/48153$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27903,27904,53770,53772,57996,58229</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10318920$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takayama, Shuichi</creatorcontrib><creatorcontrib>McDonald, J. Cooper</creatorcontrib><creatorcontrib>Ostuni, Emanuele</creatorcontrib><creatorcontrib>Liang, Michael N.</creatorcontrib><creatorcontrib>Paul J. A. Kenis</creatorcontrib><creatorcontrib>Ismagilov, Rustem F.</creatorcontrib><creatorcontrib>Whitesides, George M.</creatorcontrib><title>Patterning Cells and Their Environments Using Multiple Laminar Fluid Flows in Capillary Networks</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>This paper describes the use of laminar flow of liquids in capillary systems to pattern the cell culture substrate, to perform patterned cell deposition, and to pattern the cell culture media. We demonstrate the patterning of the cell culture substrate with different proteins, the patterning of different types of cells adjacent to each other, the patterned delivery of chemicals to adhered cells, and performing enzymatic reactions over select cells or over a portion of a cell. This method offers a way to simultaneously control the characteristics of the surface to which cells are attached, the type of cells that are in their vicinity, and the kind of media that cells or part of a cell are exposed to. The method is experimentally simple, highly adaptable, and requires no special equipment except for an elastomeric relief that can be readily prepared by rapid prototyping.</description><subject>Animals</subject><subject>Biological Sciences</subject><subject>Cattle</subject><subject>Cell Adhesion</subject><subject>Cell biology</subject><subject>Cell culture techniques</subject><subject>Cell Culture Techniques - methods</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>Cellular biology</subject><subject>Chemical suspensions</subject><subject>Culture media</subject><subject>Culture Media - chemistry</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Erythrocytes</subject><subject>Erythrocytes - metabolism</subject><subject>Escherichia coli - metabolism</subject><subject>Flow velocity</subject><subject>Fluid dynamics</subject><subject>Fluid flow</subject><subject>Fluorescence</subject><subject>Inlets</subject><subject>Laminar flow</subject><subject>Liquids</subject><subject>Physical Sciences</subject><subject>Proteins - metabolism</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkc9vFCEUx4mxsdvq2cSDIT3U02xhYBhIvJhNq03WH4f2jMwM07IyMAWmrf-9THatq_ECeXmf7-PL-wLwGqMlRjU5G52KS8FysawqWj0DC4wELhgV6DlYIFTWBaclPQRHMW4QQqLi6AU4xIhgLkq0AN-_qZR0cMbdwJW2NkLlOnh1q02A5-7eBO8G7VKE13FGPk82mdFquFaDcSrACzuZLp_-IULj4EqNxloVfsIvOj348CO-BAe9slG_2t3H4Pri_Gr1qVh__Xi5-rAuWsp5KliPS0qEYkS1vSBtUzdMlYwj1qgGad51LSYEUcYEJ5qiBmPFVVNWoiUV0RU5Bu-3c8epGXTXZtNBWTkGM2Q70isj_-44cytv_L0s8yZYlp_u5MHfTTomOZjY5oUop_0UJRN1VdZifufkH3Djp-Dy12SJMGGcEp6hsy3UBh9j0P2TD4zkHJycg5OCzfUcXFa83be_x2-TysC7HTArf7f_TJD9ZG3Sj2lv1P_JDLzZApuYfHgiKMcVIb8AhlG2mA</recordid><startdate>19990511</startdate><enddate>19990511</enddate><creator>Takayama, Shuichi</creator><creator>McDonald, J. 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| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Biological Sciences Cattle Cell Adhesion Cell biology Cell culture techniques Cell Culture Techniques - methods Cells Cells, Cultured Cellular biology Chemical suspensions Culture media Culture Media - chemistry Endothelium, Vascular - metabolism Erythrocytes Erythrocytes - metabolism Escherichia coli - metabolism Flow velocity Fluid dynamics Fluid flow Fluorescence Inlets Laminar flow Liquids Physical Sciences Proteins - metabolism |
| title | Patterning Cells and Their Environments Using Multiple Laminar Fluid Flows in Capillary Networks |
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