Evidence that neuroepithelial endocrine cells control the spontaneous tone in guinea pig tracheal preparations
Department of Physiology and Neuroscience, Lund University, S-223 62 Lund, Sweden The hypothesis that neuroepithelial endocrine (NEE) cells control spontaneous tone in isolated guinea pig tracheal preparations was examined. Epithelium-denuded preparations were unable to develop a normal oscillating...
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| Veröffentlicht in: | Journal of applied physiology (1985) 1999-03, Vol.86 (3), p.789-798 |
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| container_title | Journal of applied physiology (1985) |
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| creator | Skogvall, Staffan Korsgren, Magnus Grampp, Wolfgang |
| description | Department of Physiology and Neuroscience, Lund University, S-223 62 Lund, Sweden
The hypothesis that neuroepithelial endocrine (NEE) cells
control spontaneous tone in isolated guinea pig tracheal preparations was examined. Epithelium-denuded preparations were unable to develop a
normal oscillating tone in 12% oxygen (corresponding to systemic arterial oxygen levels) and, instead, developed a strong, smooth tone,
similar to the "classic" tone in 94% oxygen.
Inhibition of the hydrogen peroxide-producing NADPH oxidase in the NEE
cells by 20 µM diphenyleneiodonium chloride transformed, in intact
preparations in 94% oxygen, the tone from a strong, smooth type to an
oscillating tone of considerably less force. Similar experiments in
denuded preparations showed no change of tone and no oscillations.
After pretreatment with the catalase inhibitor 3-amino-1,2,4-triazole (1 mM), addition of 2 mM hydrogen peroxide to intact preparations displaying the oscillating tone caused a transformation to a strong, smooth type. These findings support the hypothesis that the spontaneous tone in this preparation is largely controlled by the oxygen-sensing NEE cells. For the first time, previous findings on isolated cells can
be linked to effects in intact tissue preparations. The results also
suggest that the regulation by the NEE cells involves the release of
powerful relaxing and contracting factors from the epithelium.
epithelium denudation; hydrogen peroxide; diphenyleneiodonium
chloride; oscillating spontaneous tone; epithelium-derived relaxing
factor |
| doi_str_mv | 10.1152/jappl.1999.86.3.789 |
| format | Article |
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The hypothesis that neuroepithelial endocrine (NEE) cells
control spontaneous tone in isolated guinea pig tracheal preparations was examined. Epithelium-denuded preparations were unable to develop a
normal oscillating tone in 12% oxygen (corresponding to systemic arterial oxygen levels) and, instead, developed a strong, smooth tone,
similar to the "classic" tone in 94% oxygen.
Inhibition of the hydrogen peroxide-producing NADPH oxidase in the NEE
cells by 20 µM diphenyleneiodonium chloride transformed, in intact
preparations in 94% oxygen, the tone from a strong, smooth type to an
oscillating tone of considerably less force. Similar experiments in
denuded preparations showed no change of tone and no oscillations.
After pretreatment with the catalase inhibitor 3-amino-1,2,4-triazole (1 mM), addition of 2 mM hydrogen peroxide to intact preparations displaying the oscillating tone caused a transformation to a strong, smooth type. These findings support the hypothesis that the spontaneous tone in this preparation is largely controlled by the oxygen-sensing NEE cells. For the first time, previous findings on isolated cells can
be linked to effects in intact tissue preparations. The results also
suggest that the regulation by the NEE cells involves the release of
powerful relaxing and contracting factors from the epithelium.
epithelium denudation; hydrogen peroxide; diphenyleneiodonium
chloride; oscillating spontaneous tone; epithelium-derived relaxing
factor</description><identifier>ISSN: 8750-7587</identifier><identifier>EISSN: 1522-1601</identifier><identifier>DOI: 10.1152/jappl.1999.86.3.789</identifier><identifier>PMID: 10066687</identifier><identifier>CODEN: JAPHEV</identifier><language>eng</language><publisher>Bethesda, MD: Am Physiological Soc</publisher><subject>Air breathing ; Animals ; Biological and medical sciences ; Carbon Dioxide - pharmacology ; Endocrine Glands - cytology ; Endocrine Glands - innervation ; Endocrine Glands - physiology ; Epithelial Cells - physiology ; Fundamental and applied biological sciences. Psychology ; Guinea Pigs ; Hydrogen Peroxide - metabolism ; Hydrogen-Ion Concentration ; Immunohistochemistry ; In Vitro Techniques ; Male ; Microscopy, Fluorescence ; Muscle Tonus - drug effects ; Muscle Tonus - physiology ; Muscle, Smooth - drug effects ; Muscle, Smooth - innervation ; Muscle, Smooth - physiology ; Oxygen - pharmacology ; Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics ; Signal Transduction - drug effects ; Signal Transduction - physiology ; Trachea - drug effects ; Trachea - innervation ; Trachea - physiology ; Vertebrates: respiratory system</subject><ispartof>Journal of applied physiology (1985), 1999-03, Vol.86 (3), p.789-798</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-b97c6a7050f6869ef559fdb8ba12e251425743507c548327b2076bc0782ea1a33</citedby><cites>FETCH-LOGICAL-c406t-b97c6a7050f6869ef559fdb8ba12e251425743507c548327b2076bc0782ea1a33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3039,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1728013$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10066687$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Skogvall, Staffan</creatorcontrib><creatorcontrib>Korsgren, Magnus</creatorcontrib><creatorcontrib>Grampp, Wolfgang</creatorcontrib><title>Evidence that neuroepithelial endocrine cells control the spontaneous tone in guinea pig tracheal preparations</title><title>Journal of applied physiology (1985)</title><addtitle>J Appl Physiol (1985)</addtitle><description>Department of Physiology and Neuroscience, Lund University, S-223 62 Lund, Sweden
The hypothesis that neuroepithelial endocrine (NEE) cells
control spontaneous tone in isolated guinea pig tracheal preparations was examined. Epithelium-denuded preparations were unable to develop a
normal oscillating tone in 12% oxygen (corresponding to systemic arterial oxygen levels) and, instead, developed a strong, smooth tone,
similar to the "classic" tone in 94% oxygen.
Inhibition of the hydrogen peroxide-producing NADPH oxidase in the NEE
cells by 20 µM diphenyleneiodonium chloride transformed, in intact
preparations in 94% oxygen, the tone from a strong, smooth type to an
oscillating tone of considerably less force. Similar experiments in
denuded preparations showed no change of tone and no oscillations.
After pretreatment with the catalase inhibitor 3-amino-1,2,4-triazole (1 mM), addition of 2 mM hydrogen peroxide to intact preparations displaying the oscillating tone caused a transformation to a strong, smooth type. These findings support the hypothesis that the spontaneous tone in this preparation is largely controlled by the oxygen-sensing NEE cells. For the first time, previous findings on isolated cells can
be linked to effects in intact tissue preparations. The results also
suggest that the regulation by the NEE cells involves the release of
powerful relaxing and contracting factors from the epithelium.
epithelium denudation; hydrogen peroxide; diphenyleneiodonium
chloride; oscillating spontaneous tone; epithelium-derived relaxing
factor</description><subject>Air breathing</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carbon Dioxide - pharmacology</subject><subject>Endocrine Glands - cytology</subject><subject>Endocrine Glands - innervation</subject><subject>Endocrine Glands - physiology</subject><subject>Epithelial Cells - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guinea Pigs</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Hydrogen-Ion Concentration</subject><subject>Immunohistochemistry</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Microscopy, Fluorescence</subject><subject>Muscle Tonus - drug effects</subject><subject>Muscle Tonus - physiology</subject><subject>Muscle, Smooth - drug effects</subject><subject>Muscle, Smooth - innervation</subject><subject>Muscle, Smooth - physiology</subject><subject>Oxygen - pharmacology</subject><subject>Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics</subject><subject>Signal Transduction - drug effects</subject><subject>Signal Transduction - physiology</subject><subject>Trachea - drug effects</subject><subject>Trachea - innervation</subject><subject>Trachea - physiology</subject><subject>Vertebrates: respiratory system</subject><issn>8750-7587</issn><issn>1522-1601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1u1DAURi0EokPhCZBQFoiuJthx_JMlqtqCVIlNWVuOczNx5bGNnQDz9jhkEN2wsiyf77vXB6G3BNeEsObjo47R1aTrulrymtZCds_Qrrw0e8IxeY52UjC8F0yKC_Qq50eMSdsy8hJdEIw551LskL_5YQfwBqp50nPlYUkBop0ncFa7CvwQTLIeKgPO5coEP6fgCgxVjuWiPYQlV3MoiPXVYSmsrqI9VHPSZoLSERNEnfRsg8-v0YtRuwxvzucl-nZ783D9eX__9e7L9af7vWkxn_d9JwzXAjM8csk7GBnrxqGXvSYNNIy0DRMtZVgY1kraiL7BgvcGC9mAJprSS_Rh640pfF8gz-po8_qFbV_FO84xp7yAdANNCjknGFVM9qjTSRGsVs3qj2a1alaSK6qK5pJ6d65f-iMMTzKb1wK8PwM6G-3GpL2x-R8nGonJuubVhk32MP20CVScTtkGFw6ndfDTifT_5O3i3AP8mtfI34SKw0h_A4EbqYc</recordid><startdate>19990301</startdate><enddate>19990301</enddate><creator>Skogvall, Staffan</creator><creator>Korsgren, Magnus</creator><creator>Grampp, Wolfgang</creator><general>Am Physiological Soc</general><general>American Physiological Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990301</creationdate><title>Evidence that neuroepithelial endocrine cells control the spontaneous tone in guinea pig tracheal preparations</title><author>Skogvall, Staffan ; Korsgren, Magnus ; Grampp, Wolfgang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-b97c6a7050f6869ef559fdb8ba12e251425743507c548327b2076bc0782ea1a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Air breathing</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carbon Dioxide - pharmacology</topic><topic>Endocrine Glands - cytology</topic><topic>Endocrine Glands - innervation</topic><topic>Endocrine Glands - physiology</topic><topic>Epithelial Cells - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guinea Pigs</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Hydrogen-Ion Concentration</topic><topic>Immunohistochemistry</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Microscopy, Fluorescence</topic><topic>Muscle Tonus - drug effects</topic><topic>Muscle Tonus - physiology</topic><topic>Muscle, Smooth - drug effects</topic><topic>Muscle, Smooth - innervation</topic><topic>Muscle, Smooth - physiology</topic><topic>Oxygen - pharmacology</topic><topic>Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics</topic><topic>Signal Transduction - drug effects</topic><topic>Signal Transduction - physiology</topic><topic>Trachea - drug effects</topic><topic>Trachea - innervation</topic><topic>Trachea - physiology</topic><topic>Vertebrates: respiratory system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Skogvall, Staffan</creatorcontrib><creatorcontrib>Korsgren, Magnus</creatorcontrib><creatorcontrib>Grampp, Wolfgang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied physiology (1985)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Skogvall, Staffan</au><au>Korsgren, Magnus</au><au>Grampp, Wolfgang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence that neuroepithelial endocrine cells control the spontaneous tone in guinea pig tracheal preparations</atitle><jtitle>Journal of applied physiology (1985)</jtitle><addtitle>J Appl Physiol (1985)</addtitle><date>1999-03-01</date><risdate>1999</risdate><volume>86</volume><issue>3</issue><spage>789</spage><epage>798</epage><pages>789-798</pages><issn>8750-7587</issn><eissn>1522-1601</eissn><coden>JAPHEV</coden><abstract>Department of Physiology and Neuroscience, Lund University, S-223 62 Lund, Sweden
The hypothesis that neuroepithelial endocrine (NEE) cells
control spontaneous tone in isolated guinea pig tracheal preparations was examined. Epithelium-denuded preparations were unable to develop a
normal oscillating tone in 12% oxygen (corresponding to systemic arterial oxygen levels) and, instead, developed a strong, smooth tone,
similar to the "classic" tone in 94% oxygen.
Inhibition of the hydrogen peroxide-producing NADPH oxidase in the NEE
cells by 20 µM diphenyleneiodonium chloride transformed, in intact
preparations in 94% oxygen, the tone from a strong, smooth type to an
oscillating tone of considerably less force. Similar experiments in
denuded preparations showed no change of tone and no oscillations.
After pretreatment with the catalase inhibitor 3-amino-1,2,4-triazole (1 mM), addition of 2 mM hydrogen peroxide to intact preparations displaying the oscillating tone caused a transformation to a strong, smooth type. These findings support the hypothesis that the spontaneous tone in this preparation is largely controlled by the oxygen-sensing NEE cells. For the first time, previous findings on isolated cells can
be linked to effects in intact tissue preparations. The results also
suggest that the regulation by the NEE cells involves the release of
powerful relaxing and contracting factors from the epithelium.
epithelium denudation; hydrogen peroxide; diphenyleneiodonium
chloride; oscillating spontaneous tone; epithelium-derived relaxing
factor</abstract><cop>Bethesda, MD</cop><pub>Am Physiological Soc</pub><pmid>10066687</pmid><doi>10.1152/jappl.1999.86.3.789</doi><tpages>10</tpages></addata></record> |
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| ispartof | Journal of applied physiology (1985), 1999-03, Vol.86 (3), p.789-798 |
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| language | eng |
| recordid | cdi_pubmed_primary_10066687 |
| source | MEDLINE; American Physiological Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Air breathing Animals Biological and medical sciences Carbon Dioxide - pharmacology Endocrine Glands - cytology Endocrine Glands - innervation Endocrine Glands - physiology Epithelial Cells - physiology Fundamental and applied biological sciences. Psychology Guinea Pigs Hydrogen Peroxide - metabolism Hydrogen-Ion Concentration Immunohistochemistry In Vitro Techniques Male Microscopy, Fluorescence Muscle Tonus - drug effects Muscle Tonus - physiology Muscle, Smooth - drug effects Muscle, Smooth - innervation Muscle, Smooth - physiology Oxygen - pharmacology Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics Signal Transduction - drug effects Signal Transduction - physiology Trachea - drug effects Trachea - innervation Trachea - physiology Vertebrates: respiratory system |
| title | Evidence that neuroepithelial endocrine cells control the spontaneous tone in guinea pig tracheal preparations |
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