Use of an E. coli pgi Knockout Strain as a Plasmid Producer

Cells were incubated overnight at 37 °C and 250 rpm and used to inoculate 250-mL baffled shake flasks containing 50 mL of complex medium (20 g/L glucose, 10 g/L bacto peptone, 10 g/L yeast extract, 3 g/L ammonium sulfate ((NH4)2SO4), 3.5 g/L potassium hydrogen phosphate (K2HPO4), 3.5 g/L potassium...

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Veröffentlicht in:	Biopharm International 2016-02, Vol.29 (2), p.38
Hauptverfasser:	Alves, Cláudia P A, Duarte, Sofia O D, Monteiro, Gabriel A, Prazeres, Duarte Miguel F
Format:	Magazinearticle
Sprache:	eng
Schlagworte:	Cell growth Chromatography Clinical trials Deoxyribonucleic acid DNA E coli Genes Glucose Manufacturing Metabolism Plasmids Potassium Proteins
Online-Zugang:	Volltext
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