Screening for yeast mutants defective in recipient ability for transkingdom conjugation with Escherichia coli revealed importance of vacuolar ATPase activity in the horizontal DNA transfer phenomenon
Proteobacterium Escherichia coli strains harboring wide-transfer-range conjugative plasmids are able to transfer these plasmids to several yeast species. Whole plasmid DNA is mobilizable in the transkingdom conjugation phenomenon. Owing to the availability of various conjugative plasmids in bacteria...
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| Veröffentlicht in: | Microbiological research 2012-05, Vol.167 (5), p.311-316 |
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| creator | Mizuta, Mami Satoh, Emi Katoh, Chika Tanaka, Katsuyuki Moriguchi, Kazuki Suzuki, Katsunori |
| description | Proteobacterium Escherichia coli strains harboring wide-transfer-range conjugative plasmids are able to transfer these plasmids to several yeast species. Whole plasmid DNA is mobilizable in the transkingdom conjugation phenomenon. Owing to the availability of various conjugative plasmids in bacteria, the horizontal DNA transfer has potential to occur between various bacteria and eukaryotes. In order to know host factor genes involved in such conjugation, we systematically tested the conjugability of strains among a yeast library comprising single-gene-knockout mutants in this study. This genome-wide screen identified 26 host chromosomal genes whose absence reduced the efficiency of the transkingdom conjugation. Among the 26 genes, 20 are responsible for vacuolar ATPase activity, while 5 genes (SHP1, CSG2, CCR4, NOT5, and HOF1) seem to play a role in maintaining the cell surface. Lack of either ZUO1 gene or SSZ1 gene, each of which encodes a component of the ribosome-associated cytoplasmic molecular chaperone, also strongly affected transkingdom conjugation. |
| doi_str_mv | 10.1016/j.micres.2011.10.001 |
| format | Article |
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Whole plasmid DNA is mobilizable in the transkingdom conjugation phenomenon. Owing to the availability of various conjugative plasmids in bacteria, the horizontal DNA transfer has potential to occur between various bacteria and eukaryotes. In order to know host factor genes involved in such conjugation, we systematically tested the conjugability of strains among a yeast library comprising single-gene-knockout mutants in this study. This genome-wide screen identified 26 host chromosomal genes whose absence reduced the efficiency of the transkingdom conjugation. Among the 26 genes, 20 are responsible for vacuolar ATPase activity, while 5 genes (SHP1, CSG2, CCR4, NOT5, and HOF1) seem to play a role in maintaining the cell surface. Lack of either ZUO1 gene or SSZ1 gene, each of which encodes a component of the ribosome-associated cytoplasmic molecular chaperone, also strongly affected transkingdom conjugation.</description><identifier>ISSN: 0944-5013</identifier><identifier>EISSN: 1618-0623</identifier><identifier>DOI: 10.1016/j.micres.2011.10.001</identifier><identifier>PMID: 22169356</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>bacteria ; Broad host range plasmid ; CCR4 receptor ; Conjugation ; Conjugation, Genetic ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - metabolism ; eukaryotic cells ; Gene transfer from bacteria to eukarya ; Gene Transfer, Horizontal ; genes ; H-transporting ATP synthase ; Horizontal gene transfer ; Interaction between bacteria and eukarya ; molecular chaperones ; mutants ; plasmids ; Plasmids - genetics ; Plasmids - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; screening ; transfer DNA ; Transformation ; Transkingdom sex ; Vacuolar Proton-Translocating ATPases - genetics ; Vacuolar Proton-Translocating ATPases - metabolism ; yeasts</subject><ispartof>Microbiological research, 2012-05, Vol.167 (5), p.311-316</ispartof><rights>2011 Elsevier GmbH</rights><rights>Copyright © 2011 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-3c733801143e14a10654022d266a268be8637cf0c74d5918e31b49a4fcd2062c3</citedby><cites>FETCH-LOGICAL-c431t-3c733801143e14a10654022d266a268be8637cf0c74d5918e31b49a4fcd2062c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micres.2011.10.001$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22169356$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mizuta, Mami</creatorcontrib><creatorcontrib>Satoh, Emi</creatorcontrib><creatorcontrib>Katoh, Chika</creatorcontrib><creatorcontrib>Tanaka, Katsuyuki</creatorcontrib><creatorcontrib>Moriguchi, Kazuki</creatorcontrib><creatorcontrib>Suzuki, Katsunori</creatorcontrib><title>Screening for yeast mutants defective in recipient ability for transkingdom conjugation with Escherichia coli revealed importance of vacuolar ATPase activity in the horizontal DNA transfer phenomenon</title><title>Microbiological research</title><addtitle>Microbiol Res</addtitle><description>Proteobacterium Escherichia coli strains harboring wide-transfer-range conjugative plasmids are able to transfer these plasmids to several yeast species. Whole plasmid DNA is mobilizable in the transkingdom conjugation phenomenon. Owing to the availability of various conjugative plasmids in bacteria, the horizontal DNA transfer has potential to occur between various bacteria and eukaryotes. In order to know host factor genes involved in such conjugation, we systematically tested the conjugability of strains among a yeast library comprising single-gene-knockout mutants in this study. This genome-wide screen identified 26 host chromosomal genes whose absence reduced the efficiency of the transkingdom conjugation. Among the 26 genes, 20 are responsible for vacuolar ATPase activity, while 5 genes (SHP1, CSG2, CCR4, NOT5, and HOF1) seem to play a role in maintaining the cell surface. Lack of either ZUO1 gene or SSZ1 gene, each of which encodes a component of the ribosome-associated cytoplasmic molecular chaperone, also strongly affected transkingdom conjugation.</description><subject>bacteria</subject><subject>Broad host range plasmid</subject><subject>CCR4 receptor</subject><subject>Conjugation</subject><subject>Conjugation, Genetic</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>eukaryotic cells</subject><subject>Gene transfer from bacteria to eukarya</subject><subject>Gene Transfer, Horizontal</subject><subject>genes</subject><subject>H-transporting ATP synthase</subject><subject>Horizontal gene transfer</subject><subject>Interaction between bacteria and eukarya</subject><subject>molecular chaperones</subject><subject>mutants</subject><subject>plasmids</subject><subject>Plasmids - genetics</subject><subject>Plasmids - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>screening</subject><subject>transfer DNA</subject><subject>Transformation</subject><subject>Transkingdom sex</subject><subject>Vacuolar Proton-Translocating ATPases - genetics</subject><subject>Vacuolar Proton-Translocating ATPases - metabolism</subject><subject>yeasts</subject><issn>0944-5013</issn><issn>1618-0623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAUhSMEokPhDRB4x2oGO_Z4kg1S1ZYfqQKktmvL49xM7pDYg-0MGl6Q1-KGFJYsLEvX3znHuqcoXgq-Elzot_vVgC5CWpVcCBqtOBePioXQolpyXcrHxYLXSi3XXMiz4llKewJUXZVPi7OyFLqWa70oft2SB3j0O9aGyE5gU2bDmK3PiTXQgst4BIaeRXB4QPCZ2S32mE9_BDlan76RvAkDc8Hvx53NGDz7gblj18l1ENF1aOmxRzI5gu2hYTgcQqQUByy07GjdGHob2cXdV5uA2Sl1iqDc3AHrQsSfwWfbs6vPF3NoC5EdOvBhoOOfF09a2yd48XCfF_fvr-8uPy5vvnz4dHlxs3RKiryUbiNlRQtTEoSyguu14mXZlFrbUldbqLTcuJa7jWrWtahAiq2qrWpdU9JSnTwv3sy-hxi-j5CyGTA56HvrIYzJ1LWsec3Xkkg1ky6GlCK05hBxsPFkBDdTg2Zv5gbN1OA0pYJI9uohYNwO0PwT_a2MgNcz0Npg7C5iMve35KBJTUw1Jb-bCaBFHBGiSY6Kc9AglZhNE_D_f_gNDDa8VQ</recordid><startdate>20120520</startdate><enddate>20120520</enddate><creator>Mizuta, Mami</creator><creator>Satoh, Emi</creator><creator>Katoh, Chika</creator><creator>Tanaka, Katsuyuki</creator><creator>Moriguchi, Kazuki</creator><creator>Suzuki, Katsunori</creator><general>Elsevier GmbH</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120520</creationdate><title>Screening for yeast mutants defective in recipient ability for transkingdom conjugation with Escherichia coli revealed importance of vacuolar ATPase activity in the horizontal DNA transfer phenomenon</title><author>Mizuta, Mami ; Satoh, Emi ; Katoh, Chika ; Tanaka, Katsuyuki ; Moriguchi, Kazuki ; Suzuki, Katsunori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-3c733801143e14a10654022d266a268be8637cf0c74d5918e31b49a4fcd2062c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>bacteria</topic><topic>Broad host range plasmid</topic><topic>CCR4 receptor</topic><topic>Conjugation</topic><topic>Conjugation, Genetic</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>eukaryotic cells</topic><topic>Gene transfer from bacteria to eukarya</topic><topic>Gene Transfer, Horizontal</topic><topic>genes</topic><topic>H-transporting ATP synthase</topic><topic>Horizontal gene transfer</topic><topic>Interaction between bacteria and eukarya</topic><topic>molecular chaperones</topic><topic>mutants</topic><topic>plasmids</topic><topic>Plasmids - genetics</topic><topic>Plasmids - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>screening</topic><topic>transfer DNA</topic><topic>Transformation</topic><topic>Transkingdom sex</topic><topic>Vacuolar Proton-Translocating ATPases - genetics</topic><topic>Vacuolar Proton-Translocating ATPases - metabolism</topic><topic>yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mizuta, Mami</creatorcontrib><creatorcontrib>Satoh, Emi</creatorcontrib><creatorcontrib>Katoh, Chika</creatorcontrib><creatorcontrib>Tanaka, Katsuyuki</creatorcontrib><creatorcontrib>Moriguchi, Kazuki</creatorcontrib><creatorcontrib>Suzuki, Katsunori</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mizuta, Mami</au><au>Satoh, Emi</au><au>Katoh, Chika</au><au>Tanaka, Katsuyuki</au><au>Moriguchi, Kazuki</au><au>Suzuki, Katsunori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening for yeast mutants defective in recipient ability for transkingdom conjugation with Escherichia coli revealed importance of vacuolar ATPase activity in the horizontal DNA transfer phenomenon</atitle><jtitle>Microbiological research</jtitle><addtitle>Microbiol Res</addtitle><date>2012-05-20</date><risdate>2012</risdate><volume>167</volume><issue>5</issue><spage>311</spage><epage>316</epage><pages>311-316</pages><issn>0944-5013</issn><eissn>1618-0623</eissn><abstract>Proteobacterium Escherichia coli strains harboring wide-transfer-range conjugative plasmids are able to transfer these plasmids to several yeast species. Whole plasmid DNA is mobilizable in the transkingdom conjugation phenomenon. Owing to the availability of various conjugative plasmids in bacteria, the horizontal DNA transfer has potential to occur between various bacteria and eukaryotes. In order to know host factor genes involved in such conjugation, we systematically tested the conjugability of strains among a yeast library comprising single-gene-knockout mutants in this study. This genome-wide screen identified 26 host chromosomal genes whose absence reduced the efficiency of the transkingdom conjugation. Among the 26 genes, 20 are responsible for vacuolar ATPase activity, while 5 genes (SHP1, CSG2, CCR4, NOT5, and HOF1) seem to play a role in maintaining the cell surface. 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| subjects | bacteria Broad host range plasmid CCR4 receptor Conjugation Conjugation, Genetic Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism eukaryotic cells Gene transfer from bacteria to eukarya Gene Transfer, Horizontal genes H-transporting ATP synthase Horizontal gene transfer Interaction between bacteria and eukarya molecular chaperones mutants plasmids Plasmids - genetics Plasmids - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism screening transfer DNA Transformation Transkingdom sex Vacuolar Proton-Translocating ATPases - genetics Vacuolar Proton-Translocating ATPases - metabolism yeasts |
| title | Screening for yeast mutants defective in recipient ability for transkingdom conjugation with Escherichia coli revealed importance of vacuolar ATPase activity in the horizontal DNA transfer phenomenon |
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