The effects of membrane cholesterol and simvastatin on red blood cell deformability and ATP release
It is known that deformation of red blood cells (RBCs) is linked to ATP release from the cells. Further, membrane cholesterol has been shown to alter properties of the cell membrane such as fluidity and bending stiffness. Membrane cholesterol content is increased in some cardiovascular diseases, for...
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| Veröffentlicht in: | Microvascular research 2012-05, Vol.83 (3), p.347-351 |
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| creator | Forsyth, Alison M. Braunmüller, Susanne Wan, Jiandi Franke, Thomas Stone, Howard A. |
| description | It is known that deformation of red blood cells (RBCs) is linked to ATP release from the cells. Further, membrane cholesterol has been shown to alter properties of the cell membrane such as fluidity and bending stiffness. Membrane cholesterol content is increased in some cardiovascular diseases, for example, in individuals with acute coronary syndromes and chronic stable angina, and therefore, because of the potential clinical relevance, we investigated the influence of altered RBC membrane cholesterol levels on ATP release. Because of the correlation between statins and reduced membrane cholesterol in vivo, we also investigated the effects of simvastatin on RBC deformation and ATP release. We found that reducing membrane cholesterol increases cell deformability and ATP release. We also found that simvastatin increases deformability by acting directly on the membrane in the absence of the liver, and that ATP release was increased for cells with enriched cholesterol after treatment with simvastatin.
► Red blood cell membrane cholesterol alters cell deformation and ATP release. ► Simvastatin directly affects the cell membrane in vitro. ► Simvastatin increased deformability and ATP release from cells with enriched cholesterol. |
| doi_str_mv | 10.1016/j.mvr.2012.02.004 |
| format | Article |
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► Red blood cell membrane cholesterol alters cell deformation and ATP release. ► Simvastatin directly affects the cell membrane in vitro. ► Simvastatin increased deformability and ATP release from cells with enriched cholesterol.</description><subject>Adenosine Triphosphate - chemistry</subject><subject>Anticholesteremic Agents - pharmacology</subject><subject>Cell Shape</subject><subject>Cell Size</subject><subject>Cholesterol - metabolism</subject><subject>Erythrocyte Count</subject><subject>Erythrocyte Deformability - drug effects</subject><subject>Erythrocyte Membrane - metabolism</subject><subject>Erythrocytes - cytology</subject><subject>Humans</subject><subject>Models, Statistical</subject><subject>Simvastatin - pharmacology</subject><issn>0026-2862</issn><issn>1095-9319</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKBDEQRYMoOj4-wI1k56rHStKv4ErEFwzoYlyHdFLBDN0dTXoG_HszjroUCmpz6lL3EHLOYM6A1Ver-bCJcw6MzyEPlHtkxkBWhRRM7pMZAK8L3tb8iByntAJgrJL8kBxxLkrJJZ8Rs3xDis6hmRINjg44dFGPSM1b6DFNGENP9Whp8sNGp0lPfqRhpBEt7foQLDXY99SiC3HQne_99PnN3yxfMtSjTnhKDpzuE5797BPyen-3vH0sFs8PT7c3i8KIik-FQQOtNF1dl5V2DbeiaVwNttOmZY3Qsjasw0rn5gaFbgFZU3LRNi2WFQgnTsjlLvc9ho91fl4NPm3fy33COikpRfbChMwk25EmhpQiOvUe_aDjp2KgtmrVSmW1aqtWQR4o883FT_q6G9D-Xfy6zMD1DsDcceMxqmQ8jgatj1mvssH_E_8FmgmKDA</recordid><startdate>201205</startdate><enddate>201205</enddate><creator>Forsyth, Alison M.</creator><creator>Braunmüller, Susanne</creator><creator>Wan, Jiandi</creator><creator>Franke, Thomas</creator><creator>Stone, Howard A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201205</creationdate><title>The effects of membrane cholesterol and simvastatin on red blood cell deformability and ATP release</title><author>Forsyth, Alison M. ; Braunmüller, Susanne ; Wan, Jiandi ; Franke, Thomas ; Stone, Howard A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-cec089cb6645af72d377f60dbac8173a96c1be5a016ce3a80e17423878e4503f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adenosine Triphosphate - chemistry</topic><topic>Anticholesteremic Agents - pharmacology</topic><topic>Cell Shape</topic><topic>Cell Size</topic><topic>Cholesterol - metabolism</topic><topic>Erythrocyte Count</topic><topic>Erythrocyte Deformability - drug effects</topic><topic>Erythrocyte Membrane - metabolism</topic><topic>Erythrocytes - cytology</topic><topic>Humans</topic><topic>Models, Statistical</topic><topic>Simvastatin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Forsyth, Alison M.</creatorcontrib><creatorcontrib>Braunmüller, Susanne</creatorcontrib><creatorcontrib>Wan, Jiandi</creatorcontrib><creatorcontrib>Franke, Thomas</creatorcontrib><creatorcontrib>Stone, Howard A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microvascular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Forsyth, Alison M.</au><au>Braunmüller, Susanne</au><au>Wan, Jiandi</au><au>Franke, Thomas</au><au>Stone, Howard A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effects of membrane cholesterol and simvastatin on red blood cell deformability and ATP release</atitle><jtitle>Microvascular research</jtitle><addtitle>Microvasc Res</addtitle><date>2012-05</date><risdate>2012</risdate><volume>83</volume><issue>3</issue><spage>347</spage><epage>351</epage><pages>347-351</pages><issn>0026-2862</issn><eissn>1095-9319</eissn><abstract>It is known that deformation of red blood cells (RBCs) is linked to ATP release from the cells. Further, membrane cholesterol has been shown to alter properties of the cell membrane such as fluidity and bending stiffness. Membrane cholesterol content is increased in some cardiovascular diseases, for example, in individuals with acute coronary syndromes and chronic stable angina, and therefore, because of the potential clinical relevance, we investigated the influence of altered RBC membrane cholesterol levels on ATP release. Because of the correlation between statins and reduced membrane cholesterol in vivo, we also investigated the effects of simvastatin on RBC deformation and ATP release. We found that reducing membrane cholesterol increases cell deformability and ATP release. We also found that simvastatin increases deformability by acting directly on the membrane in the absence of the liver, and that ATP release was increased for cells with enriched cholesterol after treatment with simvastatin.
► Red blood cell membrane cholesterol alters cell deformation and ATP release. ► Simvastatin directly affects the cell membrane in vitro. ► Simvastatin increased deformability and ATP release from cells with enriched cholesterol.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>22349292</pmid><doi>10.1016/j.mvr.2012.02.004</doi><tpages>5</tpages></addata></record> |
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| ispartof | Microvascular research, 2012-05, Vol.83 (3), p.347-351 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenosine Triphosphate - chemistry Anticholesteremic Agents - pharmacology Cell Shape Cell Size Cholesterol - metabolism Erythrocyte Count Erythrocyte Deformability - drug effects Erythrocyte Membrane - metabolism Erythrocytes - cytology Humans Models, Statistical Simvastatin - pharmacology |
| title | The effects of membrane cholesterol and simvastatin on red blood cell deformability and ATP release |
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