Human Xp/Yp telomere analysis by Southern-STELA
Telomeres are specialized structures designed to protect the ends of linear chromosomes. They are dynamic structures such that in normal somatic cells they constantly shorten as cell division progresses. There is compelling evidence that telomere shortening leads to cell senescence, a process percei...
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| Veröffentlicht in: | Biogerontology (Dordrecht) 2012-04, Vol.13 (2), p.203-213, Article 203 |
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| creator | Ivanković, Milena Ćukušić Kalajžić, Andrea Škrobot Vidaček, Nikolina Franić Šimić, Ivana Davidović Mrsić, Sanja Rubelj, Ivica |
| description | Telomeres are specialized structures designed to protect the ends of linear chromosomes. They are dynamic structures such that in normal somatic cells they constantly shorten as cell division progresses. There is compelling evidence that telomere shortening leads to cell senescence, a process perceived as the main cause of aging in higher mammals. Therefore, the features of telomere shortening are of great importance in understanding cell senescence and aging in general. By identifying unique subtelomeric regions, large enough to produce strong chemiluminescent signals, we have provided a new tool for Southern blot analysis of individual human Xp/Yp telomeres. We extend these results with quantitative fluorescence in situ hybridization using peptide nucleic acid probe (PNA Q-FISH) analysis of telomeres on the Y chromosome. Our results demonstrates unequal shortening dynamics between the p and q telomeres. |
| doi_str_mv | 10.1007/s10522-011-9368-x |
| format | Article |
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They are dynamic structures such that in normal somatic cells they constantly shorten as cell division progresses. There is compelling evidence that telomere shortening leads to cell senescence, a process perceived as the main cause of aging in higher mammals. Therefore, the features of telomere shortening are of great importance in understanding cell senescence and aging in general. By identifying unique subtelomeric regions, large enough to produce strong chemiluminescent signals, we have provided a new tool for Southern blot analysis of individual human Xp/Yp telomeres. We extend these results with quantitative fluorescence in situ hybridization using peptide nucleic acid probe (PNA Q-FISH) analysis of telomeres on the Y chromosome. Our results demonstrates unequal shortening dynamics between the p and q telomeres.</description><identifier>ISSN: 1389-5729</identifier><identifier>EISSN: 1573-6768</identifier><identifier>DOI: 10.1007/s10522-011-9368-x</identifier><identifier>PMID: 22143823</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Aging ; Biological and medical sciences ; Biomedical and Life Sciences ; Blotting, Southern - methods ; Cell Biology ; Cell culture ; Cell cycle ; Cell division ; Cells, Cultured ; Chromosomes ; Chromosomes, Human, X ; Chromosomes, Human, Y ; Development. Metamorphosis. Moult. Ageing ; Developmental Biology ; Fibroblasts - metabolism ; Fluorescence in situ hybridization ; Fluorescent indicators ; Fundamental and applied biological sciences. Psychology ; Geriatrics/Gerontology ; Humans ; In Situ Hybridization, Fluorescence ; Life Sciences ; Metaphase ; Method ; Nucleic Acid Probes ; Peptide Nucleic Acids ; Senescence ; Somatic cells ; Telomerase ; Telomere - metabolism ; Telomere Shortening ; Telomeres ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; Y chromosome ; Y chromosomes ; Yeast</subject><ispartof>Biogerontology (Dordrecht), 2012-04, Vol.13 (2), p.203-213, Article 203</ispartof><rights>Springer Science+Business Media B.V. 2011</rights><rights>2015 INIST-CNRS</rights><rights>Springer Science+Business Media B.V. 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-276810e25a45b243af73a1edc9c476bf4637a1c301c3d3d5620f07546e9643073</citedby><cites>FETCH-LOGICAL-c466t-276810e25a45b243af73a1edc9c476bf4637a1c301c3d3d5620f07546e9643073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10522-011-9368-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10522-011-9368-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25801812$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22143823$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ivanković, Milena</creatorcontrib><creatorcontrib>Ćukušić Kalajžić, Andrea</creatorcontrib><creatorcontrib>Škrobot Vidaček, Nikolina</creatorcontrib><creatorcontrib>Franić Šimić, Ivana</creatorcontrib><creatorcontrib>Davidović Mrsić, Sanja</creatorcontrib><creatorcontrib>Rubelj, Ivica</creatorcontrib><title>Human Xp/Yp telomere analysis by Southern-STELA</title><title>Biogerontology (Dordrecht)</title><addtitle>Biogerontology</addtitle><addtitle>Biogerontology</addtitle><description>Telomeres are specialized structures designed to protect the ends of linear chromosomes. They are dynamic structures such that in normal somatic cells they constantly shorten as cell division progresses. There is compelling evidence that telomere shortening leads to cell senescence, a process perceived as the main cause of aging in higher mammals. Therefore, the features of telomere shortening are of great importance in understanding cell senescence and aging in general. By identifying unique subtelomeric regions, large enough to produce strong chemiluminescent signals, we have provided a new tool for Southern blot analysis of individual human Xp/Yp telomeres. We extend these results with quantitative fluorescence in situ hybridization using peptide nucleic acid probe (PNA Q-FISH) analysis of telomeres on the Y chromosome. Our results demonstrates unequal shortening dynamics between the p and q telomeres.</description><subject>Aging</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Blotting, Southern - methods</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Cell cycle</subject><subject>Cell division</subject><subject>Cells, Cultured</subject><subject>Chromosomes</subject><subject>Chromosomes, Human, X</subject><subject>Chromosomes, Human, Y</subject><subject>Development. Metamorphosis. Moult. Ageing</subject><subject>Developmental Biology</subject><subject>Fibroblasts - metabolism</subject><subject>Fluorescence in situ hybridization</subject><subject>Fluorescent indicators</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Geriatrics/Gerontology</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Life Sciences</subject><subject>Metaphase</subject><subject>Method</subject><subject>Nucleic Acid Probes</subject><subject>Peptide Nucleic Acids</subject><subject>Senescence</subject><subject>Somatic cells</subject><subject>Telomerase</subject><subject>Telomere - metabolism</subject><subject>Telomere Shortening</subject><subject>Telomeres</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>Y chromosome</subject><subject>Y chromosomes</subject><subject>Yeast</subject><issn>1389-5729</issn><issn>1573-6768</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kMtKAzEUhoMoWi8P4EYGQXQTe3JPliJqhYILFXQ1pGlGp8ylJjNg396UVguCLkKy-P4v5_wIHRO4JABqGAkISjEQgg2TGn9uoQERimGppN5Ob6YNFoqaPbQf4wyASCrFLtqjlHCmKRug4aivbZO9zIev86zzVVv74DPb2GoRy5hNFtlj23fvPjT48elmfHWIdgpbRX-0vg_Q8-3N0_UIjx_u7q-vxthxKTtM0wAEPBWWiwnlzBaKWeKnzjiu5KTgkilLHIN0pmwqJIUClODSG8kZKHaAzlfeeWg_eh-7vC6j81VlG9_2MTeGMaKZMIm8-JckQEEzBWIpPf2Fzto-pF2XPmpAcKUTRFaQC22MwRf5PJS1DYtkype156va81R7vqw9_0yZk7W4n9R--pP47jkBZ2vARmerItjGlXHDCQ1EE5o49etzV3a2K9umC7as_h2BrpIxSZs3Hza7_R36Am0vqE8</recordid><startdate>20120401</startdate><enddate>20120401</enddate><creator>Ivanković, Milena</creator><creator>Ćukušić Kalajžić, Andrea</creator><creator>Škrobot Vidaček, Nikolina</creator><creator>Franić Šimić, Ivana</creator><creator>Davidović Mrsić, Sanja</creator><creator>Rubelj, Ivica</creator><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>0-V</scope><scope>3V.</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88J</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ALSLI</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2R</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20120401</creationdate><title>Human Xp/Yp telomere analysis by Southern-STELA</title><author>Ivanković, Milena ; Ćukušić Kalajžić, Andrea ; Škrobot Vidaček, Nikolina ; Franić Šimić, Ivana ; Davidović Mrsić, Sanja ; Rubelj, Ivica</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-276810e25a45b243af73a1edc9c476bf4637a1c301c3d3d5620f07546e9643073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Aging</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Blotting, Southern - methods</topic><topic>Cell Biology</topic><topic>Cell culture</topic><topic>Cell cycle</topic><topic>Cell division</topic><topic>Cells, Cultured</topic><topic>Chromosomes</topic><topic>Chromosomes, Human, X</topic><topic>Chromosomes, Human, Y</topic><topic>Development. Metamorphosis. Moult. Ageing</topic><topic>Developmental Biology</topic><topic>Fibroblasts - metabolism</topic><topic>Fluorescence in situ hybridization</topic><topic>Fluorescent indicators</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Geriatrics/Gerontology</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Life Sciences</topic><topic>Metaphase</topic><topic>Method</topic><topic>Nucleic Acid Probes</topic><topic>Peptide Nucleic Acids</topic><topic>Senescence</topic><topic>Somatic cells</topic><topic>Telomerase</topic><topic>Telomere - metabolism</topic><topic>Telomere Shortening</topic><topic>Telomeres</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>Y chromosome</topic><topic>Y chromosomes</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ivanković, Milena</creatorcontrib><creatorcontrib>Ćukušić Kalajžić, Andrea</creatorcontrib><creatorcontrib>Škrobot Vidaček, Nikolina</creatorcontrib><creatorcontrib>Franić Šimić, Ivana</creatorcontrib><creatorcontrib>Davidović Mrsić, Sanja</creatorcontrib><creatorcontrib>Rubelj, Ivica</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Social Sciences Premium Collection</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Social Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Social Science Premium Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Social Science Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biogerontology (Dordrecht)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ivanković, Milena</au><au>Ćukušić Kalajžić, Andrea</au><au>Škrobot Vidaček, Nikolina</au><au>Franić Šimić, Ivana</au><au>Davidović Mrsić, Sanja</au><au>Rubelj, Ivica</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Xp/Yp telomere analysis by Southern-STELA</atitle><jtitle>Biogerontology (Dordrecht)</jtitle><stitle>Biogerontology</stitle><addtitle>Biogerontology</addtitle><date>2012-04-01</date><risdate>2012</risdate><volume>13</volume><issue>2</issue><spage>203</spage><epage>213</epage><pages>203-213</pages><artnum>203</artnum><issn>1389-5729</issn><eissn>1573-6768</eissn><abstract>Telomeres are specialized structures designed to protect the ends of linear chromosomes. They are dynamic structures such that in normal somatic cells they constantly shorten as cell division progresses. There is compelling evidence that telomere shortening leads to cell senescence, a process perceived as the main cause of aging in higher mammals. Therefore, the features of telomere shortening are of great importance in understanding cell senescence and aging in general. By identifying unique subtelomeric regions, large enough to produce strong chemiluminescent signals, we have provided a new tool for Southern blot analysis of individual human Xp/Yp telomeres. We extend these results with quantitative fluorescence in situ hybridization using peptide nucleic acid probe (PNA Q-FISH) analysis of telomeres on the Y chromosome. Our results demonstrates unequal shortening dynamics between the p and q telomeres.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>22143823</pmid><doi>10.1007/s10522-011-9368-x</doi><tpages>11</tpages></addata></record> |
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| subjects | Aging Biological and medical sciences Biomedical and Life Sciences Blotting, Southern - methods Cell Biology Cell culture Cell cycle Cell division Cells, Cultured Chromosomes Chromosomes, Human, X Chromosomes, Human, Y Development. Metamorphosis. Moult. Ageing Developmental Biology Fibroblasts - metabolism Fluorescence in situ hybridization Fluorescent indicators Fundamental and applied biological sciences. Psychology Geriatrics/Gerontology Humans In Situ Hybridization, Fluorescence Life Sciences Metaphase Method Nucleic Acid Probes Peptide Nucleic Acids Senescence Somatic cells Telomerase Telomere - metabolism Telomere Shortening Telomeres Vertebrates: anatomy and physiology, studies on body, several organs or systems Y chromosome Y chromosomes Yeast |
| title | Human Xp/Yp telomere analysis by Southern-STELA |
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