Surface plasmon resonance detection of silver ions and cysteine using DNA intercalator-based amplification
We report the development of a surface plasmon resonance sensor based on the silver ion (Ag + )-induced conformational change of a cytosine-rich, single-stranded DNA for the detection of Ag + and cysteine (Cys) in aqueous solutions. In the free state, single-stranded oligonucleotides fold into doubl...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2012-03, Vol.402 (9), p.2827-2835 |
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| container_title | Analytical and bioanalytical chemistry |
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| creator | Chang, Chia-Chen Lin, Shenhsiung Wei, Shih-Chung Chu-Su, Yu Lin, Chii-Wann |
| description | We report the development of a surface plasmon resonance sensor based on the silver ion (Ag
+
)-induced conformational change of a cytosine-rich, single-stranded DNA for the detection of Ag
+
and cysteine (Cys) in aqueous solutions. In the free state, single-stranded oligonucleotides fold into double-helical structures through the addition of Ag
+
to cytosine–cytosine (C–C) mismatches. However, in the presence of Cys, which competitively binds to Ag
+
, the formation of the C–Ag
+
–C assembly is inhibited, resulting in free-state, single-stranded oligonucleotides. To enhance sensitivity, the DNA intercalator, daunorubicin, was employed to achieve signal enhancement. The detection limit for Ag
+
was 10 nM with a measurement range of 50–2,000 nM, and the detection limit for Cys was 50 nM with a measurement range of 50–2,000 nM. This simple assay was also used to individually determine the spiked Ag
+
concentration in water samples and Cys concentrations in biological fluid samples. |
| doi_str_mv | 10.1007/s00216-012-5720-5 |
| format | Article |
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+
)-induced conformational change of a cytosine-rich, single-stranded DNA for the detection of Ag
+
and cysteine (Cys) in aqueous solutions. In the free state, single-stranded oligonucleotides fold into double-helical structures through the addition of Ag
+
to cytosine–cytosine (C–C) mismatches. However, in the presence of Cys, which competitively binds to Ag
+
, the formation of the C–Ag
+
–C assembly is inhibited, resulting in free-state, single-stranded oligonucleotides. To enhance sensitivity, the DNA intercalator, daunorubicin, was employed to achieve signal enhancement. The detection limit for Ag
+
was 10 nM with a measurement range of 50–2,000 nM, and the detection limit for Cys was 50 nM with a measurement range of 50–2,000 nM. This simple assay was also used to individually determine the spiked Ag
+
concentration in water samples and Cys concentrations in biological fluid samples.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-012-5720-5</identifier><identifier>PMID: 22287048</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Analytical Chemistry ; Anthracyclines ; Assembly ; Biochemistry ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Cysteine ; Cysteine - chemistry ; Cystine ; Deoxyribonucleic acid ; DNA ; DNA, Single-Stranded - chemistry ; DNA, Single-Stranded - genetics ; Fluid dynamics ; Food Science ; Intercalating Agents - chemistry ; Ions - chemistry ; Laboratory Medicine ; Mathematical analysis ; Monitoring/Environmental Analysis ; Oligonucleotides ; Oligonucleotides - chemistry ; Oligonucleotides - genetics ; Original Paper ; Plasmons ; Sensors ; Silver ; Silver - chemistry ; Surface Plasmon Resonance - methods</subject><ispartof>Analytical and bioanalytical chemistry, 2012-03, Vol.402 (9), p.2827-2835</ispartof><rights>Springer-Verlag 2012</rights><rights>COPYRIGHT 2012 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c514t-65aacad3cd25c6d72e8dc72502f7ef0d920ce09731e56fedbd214933d17318173</citedby><cites>FETCH-LOGICAL-c514t-65aacad3cd25c6d72e8dc72502f7ef0d920ce09731e56fedbd214933d17318173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-012-5720-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-012-5720-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27915,27916,41479,42548,51310</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22287048$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, Chia-Chen</creatorcontrib><creatorcontrib>Lin, Shenhsiung</creatorcontrib><creatorcontrib>Wei, Shih-Chung</creatorcontrib><creatorcontrib>Chu-Su, Yu</creatorcontrib><creatorcontrib>Lin, Chii-Wann</creatorcontrib><title>Surface plasmon resonance detection of silver ions and cysteine using DNA intercalator-based amplification</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>We report the development of a surface plasmon resonance sensor based on the silver ion (Ag
+
)-induced conformational change of a cytosine-rich, single-stranded DNA for the detection of Ag
+
and cysteine (Cys) in aqueous solutions. In the free state, single-stranded oligonucleotides fold into double-helical structures through the addition of Ag
+
to cytosine–cytosine (C–C) mismatches. However, in the presence of Cys, which competitively binds to Ag
+
, the formation of the C–Ag
+
–C assembly is inhibited, resulting in free-state, single-stranded oligonucleotides. To enhance sensitivity, the DNA intercalator, daunorubicin, was employed to achieve signal enhancement. The detection limit for Ag
+
was 10 nM with a measurement range of 50–2,000 nM, and the detection limit for Cys was 50 nM with a measurement range of 50–2,000 nM. This simple assay was also used to individually determine the spiked Ag
+
concentration in water samples and Cys concentrations in biological fluid samples.</description><subject>Analytical Chemistry</subject><subject>Anthracyclines</subject><subject>Assembly</subject><subject>Biochemistry</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cysteine</subject><subject>Cysteine - chemistry</subject><subject>Cystine</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Single-Stranded - chemistry</subject><subject>DNA, Single-Stranded - genetics</subject><subject>Fluid dynamics</subject><subject>Food Science</subject><subject>Intercalating Agents - chemistry</subject><subject>Ions - chemistry</subject><subject>Laboratory Medicine</subject><subject>Mathematical analysis</subject><subject>Monitoring/Environmental Analysis</subject><subject>Oligonucleotides</subject><subject>Oligonucleotides - chemistry</subject><subject>Oligonucleotides - genetics</subject><subject>Original Paper</subject><subject>Plasmons</subject><subject>Sensors</subject><subject>Silver</subject><subject>Silver - chemistry</subject><subject>Surface Plasmon Resonance - methods</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkktv1TAQhSMEog_4AWyQd7BJGTvxI8urAgWpggWwtnzt8ZWvEvtiJ0j99zhK6bJl48fxN0ej8WmaNxSuKID8UAAYFS1Q1nLJoOXPmnMqqGqZ4PD84dyzs-ailCMA5YqKl80ZY0xJ6NV5c_yxZG8sktNoypQiyVhSNLEqDme0c6ha8qSE8Q9mUm-FmOiIvSszhohkKSEeyMdvOxLijNma0cwpt3tT0BEzncbggzWrzavmhTdjwdf3-2Xz6_Onn9df2tvvN1-vd7et5bSfW8GNscZ11jFuhZMMlbOScWBeogc3MLAIg-wocuHR7R2j_dB1jlZJ1eWyebf5nnL6vWCZ9RSKxXE0EdNS9FBhKjlTlXz_KEmFAOg5F8N_oz1_GqWSKimEkk-j0CkBSgha0asNPZgRdYg-zbnOqU4Kp2BTRB-qvuu72kbXqbWAbgU2p1Iyen3KYTL5rrrqNT56i4-u8dFrfPTa-tv7fpb9hO6h4l9eKsA2oNSneMCsj2nJsX7nI65_Ab_9zo4</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Chang, Chia-Chen</creator><creator>Lin, Shenhsiung</creator><creator>Wei, Shih-Chung</creator><creator>Chu-Su, Yu</creator><creator>Lin, Chii-Wann</creator><general>Springer-Verlag</general><general>Springer</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7TM</scope><scope>7UA</scope><scope>C1K</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20120301</creationdate><title>Surface plasmon resonance detection of silver ions and cysteine using DNA intercalator-based amplification</title><author>Chang, Chia-Chen ; Lin, Shenhsiung ; Wei, Shih-Chung ; Chu-Su, Yu ; Lin, Chii-Wann</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c514t-65aacad3cd25c6d72e8dc72502f7ef0d920ce09731e56fedbd214933d17318173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analytical Chemistry</topic><topic>Anthracyclines</topic><topic>Assembly</topic><topic>Biochemistry</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cysteine</topic><topic>Cysteine - chemistry</topic><topic>Cystine</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Single-Stranded - chemistry</topic><topic>DNA, Single-Stranded - genetics</topic><topic>Fluid dynamics</topic><topic>Food Science</topic><topic>Intercalating Agents - chemistry</topic><topic>Ions - chemistry</topic><topic>Laboratory Medicine</topic><topic>Mathematical analysis</topic><topic>Monitoring/Environmental Analysis</topic><topic>Oligonucleotides</topic><topic>Oligonucleotides - chemistry</topic><topic>Oligonucleotides - genetics</topic><topic>Original Paper</topic><topic>Plasmons</topic><topic>Sensors</topic><topic>Silver</topic><topic>Silver - chemistry</topic><topic>Surface Plasmon Resonance - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, Chia-Chen</creatorcontrib><creatorcontrib>Lin, Shenhsiung</creatorcontrib><creatorcontrib>Wei, Shih-Chung</creatorcontrib><creatorcontrib>Chu-Su, Yu</creatorcontrib><creatorcontrib>Lin, Chii-Wann</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Nucleic Acids Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, Chia-Chen</au><au>Lin, Shenhsiung</au><au>Wei, Shih-Chung</au><au>Chu-Su, Yu</au><au>Lin, Chii-Wann</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surface plasmon resonance detection of silver ions and cysteine using DNA intercalator-based amplification</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>402</volume><issue>9</issue><spage>2827</spage><epage>2835</epage><pages>2827-2835</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>We report the development of a surface plasmon resonance sensor based on the silver ion (Ag
+
)-induced conformational change of a cytosine-rich, single-stranded DNA for the detection of Ag
+
and cysteine (Cys) in aqueous solutions. In the free state, single-stranded oligonucleotides fold into double-helical structures through the addition of Ag
+
to cytosine–cytosine (C–C) mismatches. However, in the presence of Cys, which competitively binds to Ag
+
, the formation of the C–Ag
+
–C assembly is inhibited, resulting in free-state, single-stranded oligonucleotides. To enhance sensitivity, the DNA intercalator, daunorubicin, was employed to achieve signal enhancement. The detection limit for Ag
+
was 10 nM with a measurement range of 50–2,000 nM, and the detection limit for Cys was 50 nM with a measurement range of 50–2,000 nM. This simple assay was also used to individually determine the spiked Ag
+
concentration in water samples and Cys concentrations in biological fluid samples.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>22287048</pmid><doi>10.1007/s00216-012-5720-5</doi><tpages>9</tpages></addata></record> |
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| ispartof | Analytical and bioanalytical chemistry, 2012-03, Vol.402 (9), p.2827-2835 |
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| subjects | Analytical Chemistry Anthracyclines Assembly Biochemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Cysteine Cysteine - chemistry Cystine Deoxyribonucleic acid DNA DNA, Single-Stranded - chemistry DNA, Single-Stranded - genetics Fluid dynamics Food Science Intercalating Agents - chemistry Ions - chemistry Laboratory Medicine Mathematical analysis Monitoring/Environmental Analysis Oligonucleotides Oligonucleotides - chemistry Oligonucleotides - genetics Original Paper Plasmons Sensors Silver Silver - chemistry Surface Plasmon Resonance - methods |
| title | Surface plasmon resonance detection of silver ions and cysteine using DNA intercalator-based amplification |
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