Rapid sample preparation procedure for determination of retinol and α-tocopherol in human breast milk
► The LLE procedure for human breast milk investigation was developed and validated. ► Extracted vitamins, retinol and α-tocopherol, were separated by HPLC. ► Developed extraction procedure was applied on 120 human breast milk samples. The liposoluble vitamins (retinol and α-tocopherol) concentratio...
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| Veröffentlicht in: | Talanta (Oxford) 2012-05, Vol.93, p.147-152 |
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| creator | Kašparová, Markéta Plíšek, Jiří Solichová, Dagmar Krčmová, Lenka Kučerová, Barbora Hronek, Miloslav Solich, Petr |
| description | ► The LLE procedure for human breast milk investigation was developed and validated. ► Extracted vitamins, retinol and α-tocopherol, were separated by HPLC. ► Developed extraction procedure was applied on 120 human breast milk samples.
The liposoluble vitamins (retinol and α-tocopherol) concentration in human breast milk is of a cardinal knowledge especially for nutrition of prematurely born. It enables the feeding optimization of these important micronutrients for preterm infants.
The novel rapid liquid–liquid extraction procedure for human breast milk investigation was developed and validated according to FDA guidelines. The recovery of retinol was 82–90% measured at three concentration levels 1.0, 2.5 and 5.0μmol/L, for α-tocopherol 92–109% at concentration levels 2.5, 5.0 and 10.0μmol/L. The repeatability of extraction procedure expressed as relative standard deviation was 3.26% for retinol and 4.79% for α-tocopherol.
Developed extraction procedure was applied on 120 human breast milk samples. The separation of vitamins was completed using advantages of a monolithic column which accomplished demands of acceleration made by modern bio-analytical HPLC methodology. The analytes of interest were detected by diode-array detector at wavelengths 325nm for retinol and 290nm for α-tocopherol. |
| doi_str_mv | 10.1016/j.talanta.2012.01.065 |
| format | Article |
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The liposoluble vitamins (retinol and α-tocopherol) concentration in human breast milk is of a cardinal knowledge especially for nutrition of prematurely born. It enables the feeding optimization of these important micronutrients for preterm infants.
The novel rapid liquid–liquid extraction procedure for human breast milk investigation was developed and validated according to FDA guidelines. The recovery of retinol was 82–90% measured at three concentration levels 1.0, 2.5 and 5.0μmol/L, for α-tocopherol 92–109% at concentration levels 2.5, 5.0 and 10.0μmol/L. The repeatability of extraction procedure expressed as relative standard deviation was 3.26% for retinol and 4.79% for α-tocopherol.
Developed extraction procedure was applied on 120 human breast milk samples. The separation of vitamins was completed using advantages of a monolithic column which accomplished demands of acceleration made by modern bio-analytical HPLC methodology. The analytes of interest were detected by diode-array detector at wavelengths 325nm for retinol and 290nm for α-tocopherol.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2012.01.065</identifier><identifier>PMID: 22483891</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adult ; alpha-Tocopherol - analysis ; alpha-Tocopherol - isolation & purification ; Analytic Sample Preparation Methods - methods ; Analytical chemistry ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Exact sciences and technology ; Female ; Human breast milk ; Humans ; Indicators and Reagents - chemistry ; LLE ; Milk Proteins - chemistry ; Milk Proteins - isolation & purification ; Milk, Human - chemistry ; Monolithic column ; Other chromatographic methods ; Reproducibility of Results ; Retinol ; Sample preparation ; Time Factors ; Vitamin A - analysis ; Vitamin A - isolation & purification ; α-Tocopherol</subject><ispartof>Talanta (Oxford), 2012-05, Vol.93, p.147-152</ispartof><rights>2012 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-55b4b3fcb381c9fadbbef927cfbba211a58102a2a2de4ad9466d94234fd5fe1d3</citedby><cites>FETCH-LOGICAL-c394t-55b4b3fcb381c9fadbbef927cfbba211a58102a2a2de4ad9466d94234fd5fe1d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.talanta.2012.01.065$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25810969$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22483891$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kašparová, Markéta</creatorcontrib><creatorcontrib>Plíšek, Jiří</creatorcontrib><creatorcontrib>Solichová, Dagmar</creatorcontrib><creatorcontrib>Krčmová, Lenka</creatorcontrib><creatorcontrib>Kučerová, Barbora</creatorcontrib><creatorcontrib>Hronek, Miloslav</creatorcontrib><creatorcontrib>Solich, Petr</creatorcontrib><title>Rapid sample preparation procedure for determination of retinol and α-tocopherol in human breast milk</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>► The LLE procedure for human breast milk investigation was developed and validated. ► Extracted vitamins, retinol and α-tocopherol, were separated by HPLC. ► Developed extraction procedure was applied on 120 human breast milk samples.
The liposoluble vitamins (retinol and α-tocopherol) concentration in human breast milk is of a cardinal knowledge especially for nutrition of prematurely born. It enables the feeding optimization of these important micronutrients for preterm infants.
The novel rapid liquid–liquid extraction procedure for human breast milk investigation was developed and validated according to FDA guidelines. The recovery of retinol was 82–90% measured at three concentration levels 1.0, 2.5 and 5.0μmol/L, for α-tocopherol 92–109% at concentration levels 2.5, 5.0 and 10.0μmol/L. The repeatability of extraction procedure expressed as relative standard deviation was 3.26% for retinol and 4.79% for α-tocopherol.
Developed extraction procedure was applied on 120 human breast milk samples. The separation of vitamins was completed using advantages of a monolithic column which accomplished demands of acceleration made by modern bio-analytical HPLC methodology. The analytes of interest were detected by diode-array detector at wavelengths 325nm for retinol and 290nm for α-tocopherol.</description><subject>Adult</subject><subject>alpha-Tocopherol - analysis</subject><subject>alpha-Tocopherol - isolation & purification</subject><subject>Analytic Sample Preparation Methods - methods</subject><subject>Analytical chemistry</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Exact sciences and technology</subject><subject>Female</subject><subject>Human breast milk</subject><subject>Humans</subject><subject>Indicators and Reagents - chemistry</subject><subject>LLE</subject><subject>Milk Proteins - chemistry</subject><subject>Milk Proteins - isolation & purification</subject><subject>Milk, Human - chemistry</subject><subject>Monolithic column</subject><subject>Other chromatographic methods</subject><subject>Reproducibility of Results</subject><subject>Retinol</subject><subject>Sample preparation</subject><subject>Time Factors</subject><subject>Vitamin A - analysis</subject><subject>Vitamin A - isolation & purification</subject><subject>α-Tocopherol</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMGKFDEQQIO4uOPqJyi5yJ66TTrdPZ2TyOKqsCCInkMlqbAZu5M2SQt-lj-y32SGGfUogUqovKpUHiEvOGs54-PrQ1tghlCg7RjvWsZbNg6PyI5Pe9GIYS8ekx1jQjaS9-ySPM35wBjrBBNPyGXX9ZOYJN8R9xlWb2mGZZ2RrglXSFB8DPUcDdotIXUxUYsF0-LD6S46mrD4EGcKwdKHX02JJq73mGrGB3q_LRCoTgi50MXP356RCwdzxufn_Yp8vX335eZDc_fp_cebt3eNEbIvzTDoXgtntJi4kQ6s1uhktzdOa-g4h2HirIO6LPZgZT-ONXSid3ZwyK24ItenvnX67xvmohafDc7VFMYtKykFZ6Ifp0oOJ9KkmHNCp9bkF0g_FWfqaFgd1NmwOhpWjKtquNa9PL-w6QXt36o_Sivw6gxANjC7BMH4_I87fkGOsnJvThxWHz88JpWNx1Cd-4SmKBv9f0b5DSv6n40</recordid><startdate>20120515</startdate><enddate>20120515</enddate><creator>Kašparová, Markéta</creator><creator>Plíšek, Jiří</creator><creator>Solichová, Dagmar</creator><creator>Krčmová, Lenka</creator><creator>Kučerová, Barbora</creator><creator>Hronek, Miloslav</creator><creator>Solich, Petr</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120515</creationdate><title>Rapid sample preparation procedure for determination of retinol and α-tocopherol in human breast milk</title><author>Kašparová, Markéta ; Plíšek, Jiří ; Solichová, Dagmar ; Krčmová, Lenka ; Kučerová, Barbora ; Hronek, Miloslav ; Solich, Petr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-55b4b3fcb381c9fadbbef927cfbba211a58102a2a2de4ad9466d94234fd5fe1d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>alpha-Tocopherol - analysis</topic><topic>alpha-Tocopherol - isolation & purification</topic><topic>Analytic Sample Preparation Methods - methods</topic><topic>Analytical chemistry</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Exact sciences and technology</topic><topic>Female</topic><topic>Human breast milk</topic><topic>Humans</topic><topic>Indicators and Reagents - chemistry</topic><topic>LLE</topic><topic>Milk Proteins - chemistry</topic><topic>Milk Proteins - isolation & purification</topic><topic>Milk, Human - chemistry</topic><topic>Monolithic column</topic><topic>Other chromatographic methods</topic><topic>Reproducibility of Results</topic><topic>Retinol</topic><topic>Sample preparation</topic><topic>Time Factors</topic><topic>Vitamin A - analysis</topic><topic>Vitamin A - isolation & purification</topic><topic>α-Tocopherol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kašparová, Markéta</creatorcontrib><creatorcontrib>Plíšek, Jiří</creatorcontrib><creatorcontrib>Solichová, Dagmar</creatorcontrib><creatorcontrib>Krčmová, Lenka</creatorcontrib><creatorcontrib>Kučerová, Barbora</creatorcontrib><creatorcontrib>Hronek, Miloslav</creatorcontrib><creatorcontrib>Solich, Petr</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kašparová, Markéta</au><au>Plíšek, Jiří</au><au>Solichová, Dagmar</au><au>Krčmová, Lenka</au><au>Kučerová, Barbora</au><au>Hronek, Miloslav</au><au>Solich, Petr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid sample preparation procedure for determination of retinol and α-tocopherol in human breast milk</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2012-05-15</date><risdate>2012</risdate><volume>93</volume><spage>147</spage><epage>152</epage><pages>147-152</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>► The LLE procedure for human breast milk investigation was developed and validated. ► Extracted vitamins, retinol and α-tocopherol, were separated by HPLC. ► Developed extraction procedure was applied on 120 human breast milk samples.
The liposoluble vitamins (retinol and α-tocopherol) concentration in human breast milk is of a cardinal knowledge especially for nutrition of prematurely born. It enables the feeding optimization of these important micronutrients for preterm infants.
The novel rapid liquid–liquid extraction procedure for human breast milk investigation was developed and validated according to FDA guidelines. The recovery of retinol was 82–90% measured at three concentration levels 1.0, 2.5 and 5.0μmol/L, for α-tocopherol 92–109% at concentration levels 2.5, 5.0 and 10.0μmol/L. The repeatability of extraction procedure expressed as relative standard deviation was 3.26% for retinol and 4.79% for α-tocopherol.
Developed extraction procedure was applied on 120 human breast milk samples. The separation of vitamins was completed using advantages of a monolithic column which accomplished demands of acceleration made by modern bio-analytical HPLC methodology. The analytes of interest were detected by diode-array detector at wavelengths 325nm for retinol and 290nm for α-tocopherol.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>22483891</pmid><doi>10.1016/j.talanta.2012.01.065</doi><tpages>6</tpages></addata></record> |
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| ispartof | Talanta (Oxford), 2012-05, Vol.93, p.147-152 |
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| subjects | Adult alpha-Tocopherol - analysis alpha-Tocopherol - isolation & purification Analytic Sample Preparation Methods - methods Analytical chemistry Chemistry Chromatographic methods and physical methods associated with chromatography Exact sciences and technology Female Human breast milk Humans Indicators and Reagents - chemistry LLE Milk Proteins - chemistry Milk Proteins - isolation & purification Milk, Human - chemistry Monolithic column Other chromatographic methods Reproducibility of Results Retinol Sample preparation Time Factors Vitamin A - analysis Vitamin A - isolation & purification α-Tocopherol |
| title | Rapid sample preparation procedure for determination of retinol and α-tocopherol in human breast milk |
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