Genetic engineering to enhance the Ehrlich pathway and alter carbon flux for increased isobutanol production from glucose by Saccharomyces cerevisiae

► We engineered Saccharomyces cerevisiae for increased isobutanol production. ► Ehrlich pathway was enhanced by overexpressing suitable KDC and ADH enzymes. ► Ethanol flux was altered by combining ILV2 overexpression and PDC1 disruption. The production of higher alcohols by engineered bacteria has r...

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Veröffentlicht in:	Journal of biotechnology 2012-05, Vol.159 (1-2), p.32-37
Hauptverfasser:	Kondo, Takashi, Tezuka, Hironori, Ishii, Jun, Matsuda, Fumio, Ogino, Chiaki, Kondo, Akihiko
Format:	Artikel
Sprache:	eng
Schlagworte:	acid tolerance Aerobiosis alcohol dehydrogenase Alcohol Dehydrogenase - genetics Alcohol Dehydrogenase - metabolism biosynthesis biotechnology Butanols - analysis Butanols - metabolism carbon Carbon - metabolism Carbon flux Carboxy-Lyases - genetics Carboxy-Lyases - metabolism Ehrlich pathway engineering ethanol Ethanol - analysis Ethanol - metabolism Fermentation gene overexpression genes genetic engineering Genetic Engineering - methods genetically engineered microorganisms glucose Glucose - metabolism Hydro-Lyases - genetics Hydro-Lyases - metabolism Isobutanol Metabolic Networks and Pathways - genetics pyruvate decarboxylase Pyruvate Decarboxylase - genetics Pyruvate Decarboxylase - metabolism pyruvic acid Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism valine yeasts
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